Study on integrin-mediated albumin microbubbles adherence to activated leukocytes     

ZHOU Ying  SHAN Jiang  LIU Yi-li  YOU Xiang-dong  LV Qing-hua 《园艺学报》2002,18(11):1415-1419

AIM:To investigate the mechanism responsible for albumin microbubbles adherence to activated leukocytes. METHODS: In vitro studies were performed in which activated or nonactivated leukocytes were incubated with albumin microbubbles and observed under microscopy. The suspensions of leukocytes and microbubbles which contained or absented of integrins were analyzed with flow cytometry.RESULTS: A minimum of 50cells were identified under transillumination. 5 min after microbubbles were incubated with leukocytes, the number of cells interacting with microbubbles was greater for activated cells than for nonactivated cells(20.30±2.67 vs 4.50±1.43, P <0.01).Microbubbles attached to the surface of activated leukocytes were phagocytosed and remained intact for up to 30min. Microbubble attachment was inhibited notably by blocking the leukocyte β₂-integrin Mac-1(P <0.01) and by VLA-4mAb slightly(P <0.05) CONCLUSION: The mechanism of albumin microbubbles attaching to and phagocytosed by leukocytes was due to β₂-integrin and VLA-4 mediation. Phagocytosed microbubbles can remain at the regions of inflammation for15 min, also responsible to ultrasound.  相似文献   

狂犬病毒中和抗体检测试验中病毒回归试验的重要性     

陈先国  支海兵  滕颖  吴华伟  刘金玲 《中国兽药杂志》2004,38(4):22-24

狂犬病毒中和试验的病毒回归试验表明,病毒攻毒液的实际剂量与理论剂量不完全一致.建议在新版<中华人民共和国兽用生物制品质量标准>中增加对病毒攻毒实际剂量范围的规定,以使结果的判定更为合理.  相似文献   

国产克洛素隆尿药排泄数据分析     

胡振英  史彦斌  张新国  罗永江  罗超应  尚若峰  梁纪兰  崔颖  郑继方 《中兽医医药杂志》2004,23(4):9-11

应用Kromasil-C18色谱柱(250 mm×4.6 mm,5 μm),WatersTM480型可调波长紫外检测器,0.01M磷酸钾(pH=7):乙腈(3:1)为流动相,检测波长265 nm,含量测定采用标准曲线法,建立了RP-HPLC法检测绵羊尿中克洛素隆含量的方法.方法有效性评价结果表明,尿药含量在0.01～5.0μg/ml及5.0～30.0μg/ml范围呈良好线形关系(r=0.9993、0.9995),方法平均回收率99.32%,日内、日间变异系数分别为3.91%、6.28%.尿药最低检测限0.005μg/ml.试验绵羊以7 mg/kg单剂量经静脉、肌肉及口服三种途径给药后,尿中药物浓度分析表明,克洛素隆经体内处理后主要经肾脏排泄,给药96 h内,静注经尿排泄原药为81.76%,肌注为64.03%,口服为48.50%.  相似文献   

Characteristics in the secretion of liver TNF-α induced by infusion of LPS into veins in goats     

LIU Wei-min  WU Feng-lin  MAO Xin-zhi  CHEN Wei-hua 《园艺学报》2004,20(9):1706-1709

AIM: To study the role of liver in immune regulation in experimental endotoxemia. METHODS: 17 castrated male goats were subjected to simultaneously installing catheters in jugular, hepatic and portal veins by surgery. Four days later, lipopolysaccharide (LPS) was infused in term of three groups as followings: In group ①, LPS of 20 EU (endotoxin unit, EU)·kg^-1 was infused into portal vein; In group ②, LPS of 20 EU·kg^-1 was infused into jugular vein and LPS of 1 500 EU·kg^-1 infused into jugular vein in group ③. Before and after infusion, blood samples were collected from the three veins through the catheters for 8 h.The plasma levels of TNF-α were measured by RIA. RESULTS: In group ①, the plasma TNF-α levels of hepatic and portal vein rose to peak value at 5 h, but that of the jugular vein did not changed. In group ②, the plasma TNF-α levels in hepatic vein rose to peak value at 3 h. The TNF-α levels of jugular vein rose to peak value at 1 h and the one in portal vein enhanced continuously between 0-8 h. In group ③, the plasma TNF-α levels in jugular, hepatic and portal vein rose to significant peaks at 1 h simultaneously. CONCLUSION: During experimental endotoxemia,liver showed different dynamic characteristics in TNF-α secretion according to the pathway and doses of LPS delivery.  相似文献   

我国近年来水稻重大病虫害可持续控制技术重要研究进展——非化学控害技术研究   总被引：9，自引：1，他引：9  

何忠全  毛建辉  张志涛  禹盛苗  范永坚  俞晓平  黄炳超  陈其志 《植物保护》2004,30(2):23-27

针对水稻病虫害防治中长期存在控害保产与生产无公害稻米矛盾比较突出的问题,研究提出了几种效果显著的非化学控害增产技术。其中,利用水稻遗传背景、对病虫抗感水平等差异显著的水稻品种多样性种植,控制稻瘟病的效果达42.12％～76.68％,抑制白背飞虱若虫数量增长效果明显,增产糯稻或优质稻600～1050 kg／hm²,平均增收约1 500元／hm²;稻鱼共育控制稻飞虱的效果为63.77％～86.89％,对纹枯病病株抑制率70.52％,控制稻田杂草效果为89.57％,平收获鲜鱼319.5～1 177.5 kg／hm²,水稻产量比对照区增产7.05％～10.11％;稻鸭共育控制稻飞虱效果63.73％～77.18％,控制稻螟效果30.11％,控制纹枯病效果19.33％～67.03％,对稻田杂草控制效果91.96％,减少施肥30.6％、农药59.3％,减少投入1 987.05元／hm²。  相似文献   

兰州市SO_2污染控制区优化规划方案的探讨     

王颖  袁九毅  何治 《干旱区资源与环境》2004,(Z2)

根据《大气法》第二十七条关于划定酸雨控制区和SO2 污染控制区的规定 ,为使兰州市SO2 控制区达到环境质量目标的要求 ,本文以 98年的污染源资料和气象资料为模拟对象 ,利用大气扩散模式 ,通过各个污染源对每个控制点浓度分担率的计算 ,以污染源的总削减两最小 (总排放量最大 )或总削减率最小为优化目标 ,列出了几种优化方案并利用线性规划的方法对各种结果进行了对比分析。为了使最终的优化结果切实可行 ,将点源的上界乘一经济技术指标值 ,既可以使控制区达到环境质量目标值 ,又充分体现了公平合理的原则。  相似文献   

Osmolarity,cell volume and proliferation in nasopharyngeal carcinoma cells     

WANG Li-wei  CHEN Li-xin  MAO Jian-wen  ZHU Lin-yan  NIE Si-huai  ZHONG Ping  SUN Xue-rong  CAI Bo  LI Pan 《园艺学报》2004,20(8):1349-1352

AIM: To investigate the relationship between osmolarity, cell volume and cell proliferation in nasopharyngeal carcinoma cells. METHODS: MTT method was applied to detect the proliferation ability of the poorly-differentiated nasopharyngeal carcinoma cell (CNE-2Z) under various osmolarity conditions. The flow cytometry was used to analyse cell cycle distribution. Cell volume was obtained by the image analysis of living cells and cell viability was determined by the trypan blue assay. RESULTS: Cultivation of cells under the hypertonic conditions of 370 and 440 mOsmol/L increased cell volume by 8.7% and 27.8% and facilitated cell proliferation by 22.2% and 33.9%, respectively. However, hypotonic incubation of cells with osmolarity of 160 and 230 mOsmol/L decreased cell volume by 12.8% and 4.1% and inhibited cell proliferation by 34.0% and 15.6%, respectively. Cell volume was positively correlated with cell proliferation rate. Long-term cultivation of cells under anisotonic conditions did not significantly alter cell cycle distribution, but hypotonic cultivation decreased cell viability. CONCLUSION: Proliferation of nasopharyngeal carcinoma cells was closely correlated with the osmolarity of culture medium and cell volume. Hypotonic cultivation may inhibit cell proliferation by decreasing cell volume to facilitate cell death mechanisms.  相似文献   

水牛附睾尾精子的体外受精     

谢英  单天锡  李跃民  张家骅 《中国兽医学报》2004,24(2):190-192

采用肝素诱导获能 ,比较了 TAL P液和 BO液处理水牛附睾尾精子进行体外受精和细管冷冻精液体外受精的效果。结果表明 ,用 BO液和 TAL P液分别处理水牛附睾尾精子 ,受精后的卵裂率分别为 4 6 .6 7%和 5 3.73% ,发育率分别为 2 1.6 7%和 2 6 .87% ,其受精效果差异不显著。综合 2种方法 ,水牛附睾尾精子的受精率为 5 7.14 % ,受精后的卵裂率为 5 0 .39% ;发育率相对于培养卵为 2 4 .4 1% ,相对于卵裂卵为 4 8.4 4 % ;与细管冷冻精液 (5 6 .0 0 % ,5 4 .31% ,2 6 .72 % ,4 9.2 1% )相比 ,差异不显著。形态学观察还表明 ,用保温干储的方法可获得活率好、存活时间长的附睾尾精子。试验结果说明水牛附睾尾精子用于体外受精可以得到与细管冷冻精液相当的效果。  相似文献   

家鸡Leptin成熟肽cDNA的克隆、重组蛋白表达及纯化   总被引：4，自引：0，他引：4  

施振旦  邵西兵  方梅霞  于迎春  刘颖  陈楠 《中国兽医学报》2004,24(3):258-260

从 18周龄鸡卵巢组织中抽提总 RNA,使用六聚体随机引物反转录后 ,用鸡 L eptin(瘦素 )特异性引物扩增出鸡L eptin编码区第 5 2～ 4 6 0 bp的长度为 4 0 9bp的 c DNA片段。根据鸡 L eptin的 3′端第 4 6 0～ 4 92 bp序列设计了 3条部分相互重叠并且顺序串联延伸的下游反义引物 ,并在最后 1条引物 3′端连接上 Eco R 切点 ;在以上用于反转录扩增的 5′端引物的 5′端连接一 Bam H 切点。用该 5′端引物分别与 3个 3′端引物配对 ,利用反转录扩增出的 4 0 9bp的L eptin c DNA片段作为第 1模板进行扩增 ,扩增产物再作为模板与下一引物对再次扩增。经 3次扩增得到编码鸡 L ep-tin成熟肽的全长 4 5 1bp的 c DNA序列。将该 4 5 1bp的 L eptin c DNA序列经 Bam H 和 Eco R 双酶切后 ,克隆入表达质粒 p RSET A的 Bam H 和 Eco R 两酶切位点之间 ,构建成表达质粒 p L ep- SCAU。转化有重组表达质粒 p L ep-SCAU的大肠杆菌 BL 2 1(DE3)在 L B培养基中培养后 ,经 IPTG诱导表达出相对分子质量为 2 0 10 0的鸡 L eptin融合蛋白和少量 4 0 2 0 0的 L eptin融合蛋白。L eptin融合蛋白的表达在 IPTG浓度为 0 .0 5 mmol/ L 时达到最高 ,占总菌体蛋白的 32 .6 %。用 Ni- NTA凝胶从 7L 发酵培养菌裂解液中纯化出 180 m g左右  相似文献   

猪伪狂犬病抗体免疫金标快速检测试纸法   总被引：2，自引：0，他引：2  

黄印尧  张长弓  孔繁德  方莹  颜江华 《福建畜牧兽医》2004,26(3):8-10

应用免疫学原理与胶体金层析技术相结合，采用双抗原夹心法建立了检测猪伪狂犬病抗体水平的“猪伪狂犬病抗体免疫胶体金快速检测试纸法”，用该法与美国Idexx公司的猪伪狂犬病ELISA试剂盒，同时对16纷猪血液或血清进行抗体水平检测，符合率达100％。同时使用金标法对100份猪血液和对应血清进行检测，结果也相同。试验结果显示．金标法与ELISA一样，具有微量、特异、准确等优点，且操作简易，而金标法不需要任何仪器，检测时间短．结果直观，容易判定，适用于基层兽医站、养猪场使用和大面积猪伪狂犬病抗体普查。  相似文献