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41.
The spatial, temporal, and spatio-temporal features of the 2001 British foot-and-mouth disease epidemic in selected areas within the counties of Cumbria and Devon, which experienced the greatest incidence of disease, are described using hazard functions, extraction mapping and the space-time K-function. In Cumbria, the hazard of foot-and-mouth disease infection peaked at 2.8% in the week commencing 8 March 2001 and farm holdings in this area continued to be identified with disease to 12 September 2001. In contrast, peak infection hazard in Devon was 0.7% in the week commencing 15 March 2001 and eradication of the disease was achieved in this area by 31 May 2001. Persistence of the disease in Cumbria was consistent with: (1) many cattle holdings infected early in the epidemic (creating a high environmental viral load), and (2) a relatively large amount of medium-to-long-distance spread of the virus associated with seasonal farming activities-compounded to some extent by the movement of people and vehicles between disaggregated farm land parcels. The interaction of disease risk in Cumbria showed that premises remained infectious for longer throughout May, June and July, consistent with delays in disease detection during this period. 相似文献
42.
Evaluation of single-nucleotide polymorphisms in CAPN1 for association with meat tenderness in cattle 总被引:24,自引:0,他引:24
Page BT Casas E Heaton MP Cullen NG Hyndman DL Morris CA Crawford AM Wheeler TL Koohmaraie M Keele JW Smith TP 《Journal of animal science》2002,80(12):3077-3085
Micromolar calcium activated neutral protease (CAPN1) was evaluated as a candidate gene for a quantitative trait locus (QTL) on BTA29 affecting meat tenderness by characterization of nucleotide sequence variation in the gene. Single-nucleotide polymorphisms (SNP) were identified by sequencing all 22 exons and 19 of the 21 introns in two sires (Piedmontese x Angus located at the U.S. Meat Animal Research Center in Clay Center, NE; Jersey x Limousin located at AgResearch in New Zealand) of independent resource populations previously shown to be segregating meat tenderness QTL on BTA29. The majority of the 38 SNP were found in introns or were synonymous substitutions in the coding regions, with two exceptions. Exons 14 and 9 contained SNP that were predicted to alter the protein sequence by the substitution of isoleucine for valine in Domain III of the protein, and alanine for glycine in Domain II of the protein. The resource populations were genotyped for these two SNP in addition to six intronic polymorphisms and two silent substitutions. Analysis of genotypes and shear force values in both populations revealed a difference between paternal CAPN1 alleles in which the allele encoding isoleucine at position 530 and glycine at position 316 associated with decreased meat tenderness (increased shear force values) relative to the allele encoding valine at position 530 and alanine at position 316 (P < 0.05). The association of maternal alleles with meat tenderness phenotypes is consistent with the hypothesis of CAPN1 as the gene underlying the QTL effect in two independent resource populations and presents the possibility of using these markers for selective breeding to reduce the numbers of animals with unfavorable meat tenderness traits. 相似文献
43.
Phillips CJ Foster CR Morris PA Teverson R 《Animal health research reviews / Conference of Research Workers in Animal Diseases》2002,3(1):3-13
Genetic variation in the susceptibility of cattle to Mycobacterium bovis infection exists in differences between families and species, but not breeds. Susceptibility to M. bovis infection increases with age of cattle. Natural exposure to M. bovis or environmental mycobacteria may assist in the development of specific immunity, but there is no direct evidence for such immunological priming of tuberculosis resistance in cattle. This has, however, been demonstrated in humans and other animals. Since non-specific mechanisms have a role in protective immunity, developing an effective vaccine will be difficult, even though some protection of other species has been achieved. Immunological suppression in the periparturient period can produce anergic reactors, which may act as a constant source of infection for cattle-to-cattle transmission. Circumstantial evidence suggests that an adequate intake of mineral, vitamin and protein reduces the susceptibility of cattle. Although weather patterns have been implicated in the susceptibility of herds to M. bovis infection, there is insufficient information to determine the risk factors precisely. It is concluded that some reduction in the susceptibility of cattle to M. bovis infection can be achieved by modifications to the management system to minimize risk factors, but that a considerable amount of further research is required. 相似文献
44.
A low-selenium diet increases thyroxine and decreases 3,5,3'triiodothyronine in the plasma of kittens 总被引:1,自引:0,他引:1
Yu S Howard KA Wedekind KJ Morris JG Rogers QR 《Journal of animal physiology and animal nutrition》2002,86(1-2):36-41
The effect of a low-selenium diet on thyroid hormone metabolism was investigated in growing kittens. Twelve specific-pathogen-free kittens with ages ranging from 16 to 18 weeks were divided into two groups of equal number with equal sex distribution in each group. One group was fed a yeast-based low-selenium diet (0.02 mg Se/kg diet) while the other group was fed the same diet supplemented with Na2 SeO3 at 0.4 mg Se/kg diet for 8 weeks. Food intake, body weight and body weight gain were not affected by the low-Se diet during the study period. However, kittens given the low-Se diet had significantly reduced plasma selenium concentration and glutathione peroxidase activity. Plasma total thyroxine (T4) increased and total 3,5,3'triiodothyronine (T3) decreased significantly in kittens fed the low-Se diet at the end of the study. These results suggest that type I deiodinase in cats is a selenoprotein- or a selenium-dependent enzyme. 相似文献
45.
Kirker-Head CA Chandna VK Agarwal RK Morris EA Tidwell A O'Callaghan MW Rand W Kumar MS 《American journal of veterinary research》2000,61(6):714-718
OBJECTIVE: To correlate substance P content of synovial fluid with prostaglandin E2 content, radiographic evidence of joint abnormality, and anatomic location of the joint for normal and osteoarthritic joints of horses. SAMPLE POPULATION: Synovial fluid from 46 normal joints in 21 horses and 16 osteoarthritic joints in 10 horses. PROCEDURE: Normal and osteoarthritic joints were identified by clinical and radiographic examination, by response to nerve blocks, during scintigraphy or surgery, or by clinicopathologic evaluation. Substance P and prostaglandin E2 contents of synovial fluid were determined by radioimmunoassay. Radio-graphs of joints were assigned a numeric score reflecting severity of lesions. Joints were assigned a numeric score reflecting anatomic location. RESULTS: Median concentrations of substance P and prostaglandin E2 were significantly increased in osteoarthritic joints, compared with normal joints. A significant correlation was found between concentrations of substance P and prostaglandin E2 in synovial fluid, but a correlation was not detected between substance P concentration in synovial fluid and anatomic location of the joint or between radiographic scores of osteoarthritic joints and concentrations of substance P or prostaglandin E2. CONCLUSIONS AND CLINICAL RELEVANCE: A correlation existed between concentrations of substance P and prostaglandin E2 in synovial fluid obtained from normal and osteoarthritic joints. However, content of substance P in synovial fluid cannot be predicted by the radiographic appearance of the joint or its anatomic location. Substance P and prostaglandin E2 may share an important and related role in the etiopathogenesis of osteoarthritis, lending credence to the importance of neurogenic inflammation in horses. 相似文献
46.
Ultraviolet radiation and laying pullets 总被引:1,自引:1,他引:0
1. Responses to ultraviolet (UV) radiation were studied in two trials. In one trial, sexually mature pullets, that had been maintained on an 8L:16D regimen from 2 d of age, were exposed sequentially, for periods of 9 to 12 d, to a further 8 h of very dim visible light (VDV), to 8 h of UV radiation and, finally, to an extra 8 h of normal light (conventional 16L:8D). Individual ovipositions were recorded during the last 48 h of each treatment. In the second trial, sexually mature pullets which had been allowed to 'free-run' for 14 d under continuous normal illumination (LL), were given, in addition to the normal light, a 12-h period of UV radiation commencing at midday or midnight for a further 15 d. During the final 48 h oviposition times were recorded and 4 food intakes for each 12-h period were determined. 2. In trial 1, mean oviposition time under VDV and UV supplementation was not significantly different from that under the 8L:16D regimen. Transfer to a 16L:8D regimen altered mean time of oviposition by about 4 h. In trial 2, eggs continued to be laid almost at random in all groups. 3. Food intake was suppressed during the 12-h period of UV supplementation compared with that when the birds were not receiving UV. 4. It is concluded that the addition of 8 h of UV radiation (at the intensity used in these studies) to 8 h of normal light does not cause a phase shift in the timing of the 'open-period' for pre-ovulatory luteinising hormone release which determines the time of oviposition. Furthermore, the insertion of 12-h periods of UV into continuous illumination does not entrain egg laying. 5. The suppressing effect of UV on food intake but lack of influence on the timing of the ovulatory cycle suggests that UV (at the intensity used in this study) acts principally at the retinal level and, as a result, stimulates only behavioural responses in laying birds. 相似文献
47.
A stochastic simulation model to investigate the transmission of classical swine fever (CSF) virus within an infected farm is described. The model is structured according to the processes that occur within and between management groups (pig units or houses). It uses the individual pig as the unit of interest and estimates the number of animals in the states 'susceptible', 'infected', 'infectious', and 'removed' for each day of the disease incident. Probabilities are assigned to the transitions between states. The probability of a pig becoming infected is made dependent on the probability of contact between a susceptible and an infectious pig as well as the probability of transmission. The more pigs become infected in one unit, the more likely is subsequent spread to another management group on the farm. Ultimately, the probability that a shipment of pigs from the farm will include at least one infected pig can be estimated in order to identify high-risk movements during a CSF epidemic. The model results were compared with experimental data on CSF transmission within one pig unit (management group). It could be shown that the model was capable of reproducing the experimentally observed infection and mortality rates. To improve the input parameters and for further model validation, more experimental data and field data from CSF outbreaks are needed. 相似文献
48.
With the cloning of the alpha-amino-3-hydroxy-5-methyl-4-isaxole propionic acid (AMPA)-type receptor subunits, it is now possible to localize these receptor subunits in the spinal cord. Comparison of the neurokinin 1 receptor distribution with that of non-N-methyl-D-aspartate glutamate receptor subunits (GluR1-4), considered to be AMPA-type, was investigated in rat spinal cord by immunocytochemical methods. Different patterns of immunolabelling were observed with the antibodies to the GluR1, GluR2/3 and GluR4 subunits in the lumbar spinal cord. Immunolabelling with antibodies to both GluR1 and GluR2/3 revealed intensive staining in the dorsal horn, while staining for GluR2/3 and GluR4 was dense in the motor neurons of the ventral horn. These results suggest that in the rat spinal cord AMPA-type receptors vary their composition according to the region where they are expressed. Neurokinin-1-receptor-expressing neurons in the dorsal horn do not appear to express the GluR4 subunit, however, whether they express the GluR1, GluR2/3 receptors subunits could not be determined. 相似文献
49.
ABSTRACT In 1993, a bacterial blight caused important losses of cantaloupe (Cucumis melo var. cantalupensis) in southwestern France and has now been reported in all cantaloupe-growing regions of France. The causal agent of this blight is Pseudomonas syringae, although on a worldwide basis this bacterium has not been a major pathogen of melon for over 50 years. To identify the pathovar of the cantaloupe pathogen, we employed biochemical tests, plasmid and chromosomal profiling, and host range studies for 23 strains from cantaloupe and 47 reference strains of 14 pathovars of P. syringae. Numerical analysis of 119 traits, serological typing, syringomycin production, and BOX-polymerase chain reaction profiles did not allow us to differentiate among pathovars related to P. syringae pv. syringae. Host range studies of cantaloupe and references strains on 18 plant species showed that virulence to sugar beet was a common feature of strains virulent on cantaloupe, but was not common to strains avirulent on cantaloupe. Virulence to other species of plants varied among strains, but the overall extent of the host range was proportional to aggressiveness to cantaloupe. We propose that the strains attacking cantaloupe in France be considered P. syringae pv. aptata and that adequate host range testing may reveal that this pathovar is the cause of cantaloupe blight reported in other parts of the world. 相似文献
50.