水资源核算及对GDP的修正——以中国东部经济发达地区为例   总被引：1，自引：0，他引：1  

王舒曼  曲福田 《南京农业大学学报》2001,24(2)

对中国东部经济发达地区水资源核算的结果表明 ,以GDP为主要衡量指标的现行国民经济核算体系过高地估计了该地区经济的发展水平。扣除水资源价值损失 ,1994～ 1997年区域GDP平均下降 5 5 8% ,这意味着东部经济发达地区经济增长存在着较为严重的环境负债  相似文献   

Immune response of chickens inoculated with a recombinant avian leukosis virus     

M A McBride  R M Shuman 《Avian diseases》1988,32(1):96-102

Specific-pathogen-free embryos (18-day incubation) and hatched chicks were inoculated with a recombinant avian leukosis virus (ALV) produced by recombinant DNA techniques. Enzyme-linked immunosorbent assays were used to measure the production of viral-protein-specific antibody and the viral protein, p27, in the serum at 2, 5, 8, 14, and 20 weeks of age. Of the inoculated chickens surviving to 20 weeks, 64% produced viral-protein-specific antibodies and 42% transiently produced the viral protein, p27. Chickens inoculated as embryos did not differ significantly from those inoculated at hatch with respect to antibody and viral protein production. Antibody production peaked at 5 weeks postinoculation and declined over the remaining 15 weeks of the study. No evidence of chronic tolerant infection or mortality due to neoplastic disease was found.  相似文献   

Osteomyelitis and arthritis induced in swine by Lancefield's group A streptococci (streptococcus pyogenes)     

R L Wood  R C Cutlip  R D Shuman 《The Cornell veterinarian》1971,61(3):457-470

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Simultaneous Flow Cytometric Analysis of Phagocytosis and Oxidative Burst Activity in Equine Leukocytes     

Flaminio MJ  Rush BR  Davis EG  Hennessy K  Shuman W  Wilkerson MJ 《Veterinary research communications》2002,26(2):85-92

This paper describes a method for simultaneously measuring phagocytosis and oxidative burst activity in equine peripheral blood leukocytes by flow cytometry. Opsonized propidium iodide-labelled Staphylococcus aureus (PI-Sa) was used to measure the uptake of bacteria by equine phacocytes and the oxidative burst activity by oxidation of dihydrorhodamine 123. The requirements to achieve optimal activity of phagocytosis and oxidative burst are described. The advantage of the simultaneous technique is that it provides both independent and comparative values for phagocytosis and the oxidative burst, for the detection of impaired mechanisms of microbial destruction. Furthermore, the technique allows evaluation of opsonization activity in this context.  相似文献   

Isotype-specific antibodies in horses and dogs with immune-mediated hemolytic anemia   总被引：1，自引：1，他引：0  

Wilkerson MJ  Davis E  Shuman W  Harkin K  Cox J  Rush B 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(2):190-196

Classes of antibody bound to erythrocytes were determined using direct immunofluorescence (DIF) flow cytometry in 3 horses and 12 dogs with immune-mediated hemolytic anemia (IMHA). Background levels of antibody binding were determined in samples from 12 horses and 12 dogs that were free of clinical disease. The range of nonspecific binding of a fluorescein isothiocyanate (FITC)-conjugated goat anti-equine immunoglobulin G (IgG) was 19.9–36.7%, but was eliminated by the use of the F(ab)₂ fragment of FITC-conjugated goat anti-equine IgG. Background binding by other class-specific antibodies to equine and canine erythrocytes was negligible. The DIF results were compared to the direct antiglobulin (Coombs) test in 5 horses and 20 dogs with anemia. The former assay was more sensitive in dogs with IMHA than was the Coombs' test (100% versus 58%). In contrast, the Coombs' test had better specificity than the DIF assay (100% versus 87.5%, respectively). Using clinical parameters or response to therapy as the comparison, the positive and negative predictive values for the DIF test were 92% and 100% compared to the values of the Coombs' test of 100% and 62%. The DIF assay detected low levels of cells bound with antibody (<30%) in 5 dogs that were Coombs' test-negative. For both species, performance of the DIF test was independent of the prozone effect. Five dogs with IMHA had IgG and IgM on erythrocytes, 5 had IgG, and 2 had IgM. Three horses had surface-bound IgG, including a horse with suspected penicillin-induced IMHA, a foal with neonatal isoerythrolysis, and a foal with clostridial septicemia. The DIF method was valuable in monitoring the response to therapy in the foal with neonatal isoerythrolysis.  相似文献   

黄淮海平原县域农村居民点布局优化及其整治策略   总被引：3，自引：6，他引：3  

周宁  郝晋珉  孟鹏  陈丽  洪舒蔓  穆哈拜提·帕热提 《农业工程学报》2015,31(7):256-263

为合理确定农村居民点的布局优化方向,科学指导土地整治和新农村建设中农村居民点的迁移合并实践,该文以河北省曲周县为例,从农村居民点自身发展条件和邻近居民点的空间相互作用关系2个角度,构建了以斑块为单元符合平原区特点的农村居民点结节性指数评价体系,利用多因素综合评价法测算了各农村居民点结节性指数值;利用引力模型确定了整理单元,总结出农村居民点间的4种空间相互作用关系,即串联型、并联型、聚合型、双核型;在此基础上,分别利用场强模型和引力模型将河北省曲周县242个主要农村居民点划分为城镇化型、重点发展型、规模控制型和迁移合并型,并有针对性的提出布局优化调控建议。研究结果符合曲周县实际情况,为当地农村居民点整理工作提供了依据,对黄淮海平原其他农区的农村居民点科学规划与布局有一定借鉴意义。  相似文献   

A cortical area selective for visual processing of the human body     

Downing PE  Jiang Y  Shuman M  Kanwisher N 《Science (New York, N.Y.)》2001,293(5539):2470-2473

Despite extensive evidence for regions of human visual cortex that respond selectively to faces, few studies have considered the cortical representation of the appearance of the rest of the human body. We present a series of functional magnetic resonance imaging (fMRI) studies revealing substantial evidence for a distinct cortical region in humans that responds selectively to images of the human body, as compared with a wide range of control stimuli. This region was found in the lateral occipitotemporal cortex in all subjects tested and apparently reflects a specialized neural system for the visual perception of the human body.  相似文献   

Human chromosome 7: DNA sequence and biology     

Scherer SW  Cheung J  MacDonald JR  Osborne LR  Nakabayashi K  Herbrick JA  Carson AR  Parker-Katiraee L  Skaug J  Khaja R  Zhang J  Hudek AK  Li M  Haddad M  Duggan GE  Fernandez BA  Kanematsu E  Gentles S  Christopoulos CC  Choufani S  Kwasnicka D  Zheng XH  Lai Z  Nusskern D  Zhang Q  Gu Z  Lu F  Zeesman S  Nowaczyk MJ  Teshima I  Chitayat D  Shuman C  Weksberg R  Zackai EH  Grebe TA  Cox SR  Kirkpatrick SJ  Rahman N  Friedman JM  Heng HH  Pelicci PG  Lo-Coco F  Belloni E  Shaffer LG  Pober B  Morton CC  Gusella JF  Bruns GA  Korf BR 《Science (New York, N.Y.)》2003,300(5620):767-772

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Bond-selective control of a heterogeneously catalyzed reaction     

Killelea DR  Campbell VL  Shuman NS  Utz AL 《Science (New York, N.Y.)》2008,319(5864):790-793

Energy redistribution, including the many phonon-assisted and electronically assisted energy-exchange processes at a gas-metal interface, can hamper vibrationally mediated selectivity in chemical reactions. We establish that these limitations do not prevent bond-selective control of a heterogeneously catalyzed reaction. State-resolved gas-surface scattering measurements show that the nu1 C-H stretch vibration in trideuteromethane (CHD3) selectively activates C-H bond cleavage on a Ni(111) surface. Isotope-resolved detection reveals a CD3:CHD2 product ratio > 30:1, which contrasts with the 1:3 ratio for an isoenergetic ensemble of CHD3 whose vibrations are statistically populated. Recent studies of vibrational energy redistribution in the gas and condensed phases suggest that other gas-surface reactions with similar vibrational energy flow dynamics might also be candidates for such bond-selective control.  相似文献   

Legionella effectors that promote nonlytic release from protozoa     

Chen J  de Felipe KS  Clarke M  Lu H  Anderson OR  Segal G  Shuman HA 《Science (New York, N.Y.)》2004,303(5662):1358-1361

Legionella pneumophila, the bacterial agent of legionnaires' disease, replicates intracellularly within a specialized vacuole of mammalian and protozoan host cells. Little is known about the specialized vacuole except that the Icm/Dot type IV secretion system is essential for its formation and maintenance. The Legionella genome database contains two open reading frames encoding polypeptides (LepA and LepB) with predicted coiled-coil regions and weak homology to SNAREs; these are delivered to host cells by an Icm/Dot-dependent mechanism. Analysis of mutant strains suggests that the Lep proteins may enable the Legionella to commandeer a protozoan exocytic pathway for dissemination of the pathogen.  相似文献