Mycoplasma hyopneumoniae colonization of pigs sired by different boars          下载免费PDF全文

Alvaro Ruiz  Lucina Galina    Carlos Pijoan 《Canadian journal of veterinary research》2002,66(2):79-85

Differences in Mycoplasma hyopneumoniae colonization were evaluated in experimentally inoculated pigs sired by 3 different boars of the same genetic line. Forty-six pigs were used, including a treatment group and positive and negative control groups. The pigs were intratracheally inoculated with an M. hyopneumoniae suspension or with Friis media as a placebo. To evaluate differences in the magnitude of colonization during a 35-day period, nasal and tracheal swabs were collected weekly and tested by nested polymerase chain reaction (N-PCR). Temperature, weight and circulating antibodies were measured for 35 days. At 11 and 35 d postinoculation the pigs were necropsied and macroscopic and microscopic lesions were determined. A section of bronchus was tested by the indirect immunofluorescence test (IFAT), scanning electron microscopy (SEM) and N-PCR. The N-PCR results from the nasal and tracheal swabs showed that the pigs sired by one boar (B3) had a distinctive colonization pattern, different from that of the pigs from the other 2 boars and from the positive controls. SEM studies demonstrated that at 35 d postinoculation a higher proportion of B3 pigs had lower numbers of mycoplasmas attached to the cilia compared with B1 and B2 offspring. No significant differences were observed in temperature and weight gain among groups by ANOVA; however, with use of a 2 × 2 table, temperature differences were observed between pigs sired by boars B1 and B2 at 4 d postinoculation. No pigs seroconverted, showed gross or microscopic lesions, or had positive IFAT results. These results provide evidence of differences in patterns of colonization between pigs sired by different boars, suggesting a possible genetic effect.  相似文献   

Capnographic documentation of nasoesophageal and nasogastric feeding tube placement in dogs   总被引：1，自引：0，他引：1  

Paula A. Johnson  DVM    F. A. Mann  DVM  MS  DACVS  DACVECC    John Dodam  DVM  MS  PhD  DACVA    Keith Branson  DVM  MS  DACVA    Colette Wagner-Mann  DVM  PhD    Mark A. Brady  DVM    Elizabeth Dunphy  DVM 《Journal of Veterinary Emergency and Critical Care》2002,12(4):227-233

Objective: To evaluate the ability of capnography to document proper placement of nasoesophageal (NE) and nasogastric (NG) feeding tubes. This study was conducted in 3 phases. Phase I of this study was designed in order to test the efficacy of capnography to distinguish placement of a feeding tube in the alimentary tract versus the respiratory tract. Phase II was designed in order to document that carbon dioxide (CO₂) could be measured through a polyvinyl chloride (PVC) feeding tube. Phase III was performed in order to evaluate the technique of continuous monitoring during insertion of the feeding tube into the esophagus and stomach as would be performed during a clinical‐tube placement. Design: Prospective study. Setting: Research laboratory. Animals: 24 adult dogs. Interventions: In Phase I, sedated dogs were instrumented with an intratracheal catheter and an 8 French feeding tube placed nasally into the distal esophagus and later advanced into the stomach. In Phase II, dogs were anesthetized and an 8 French feeding tube was placed down the endotracheal tube, then into the esophagus and later advanced into the stomach. In Phase III, sedated dogs were instrumented with an 8 French feeding tube inserted intranasally and then advanced to the level of the nasopharynx, distal esophagus and, lastly, the stomach. Fluoroscopy was used in order to determine location of the feeding tube. Measurements and main results: Phase I measurements included respiratory rate and CO₂ from the trachea, esophagus, and stomach and pH of gastric fluid sample. Phase II measurements included respiratory rate and CO₂ from the endotracheal tube, feeding tube in the endotracheal tube, feeding tube in the distal esophagus, and feeding tube in the stomach. Phase III data collection included respiratory rate and CO₂ as the tube was passed through the nasal cavity, nasopharynx, esophagus and stomach. Phase I fluid samples were collected from 5 of the 9 dogs and had pH values from 1.68 to 4.20. In both phases, values for the respiratory rate and CO₂ from the esophagus and stomach were 0 ± 0, significantly lower (P < 0.001) than the values from the trachea. In Phase II, there was no significant difference between the respiratory rates (P = 0.886) and CO₂ (P = 0.705) readings obtained from the endotracheal tube compared to readings from the feeding tube in the endotracheal tube. In Phase III, there was a significant difference (P < 0.001) between the respiratory rates and CO₂ readings obtained from the nasal cavity and the nasopharynx when compared to those readings obtained from the esophagus and stomach. Measurement of CO₂ and respiratory rate resulted in a reading of 0 every time the feeding tube was in the esophagus or stomach. Conclusions: Capnography may be used in order to detect airway placement of NE and NG tubes.  相似文献   

The Application of GIS in Small Watershed Classification in Loess Plateau     

Zhu Jinzhao  Wu Bin  Bi Huaxing  Zhou Changqing College of Soil  Water Conservation  Beijing Forestry UniversityBeijing  P.R.China 《中国林学(英文版)》1994,(2)

ＴｈｅＡｐｐｌｉｃａｔｉｏｎｏｆＧＩｓｉｎＳｍａｌｌＷａｔｅｒｓｈｅｄＣｌａｓｓｉｆｉｃａｔｉｏｎｉｎＬｏｅｓｓＰｌａｔｅａｕＺｈｕＪｉｎｚｈａｏ，ＷｕＢｉｎ，ＢｉＨｕａｘｉｎｇ，ＺｈｏｕＣｈａｎｇｑｉｎｇＣｏｌｌｅｇｅｏｆＳｏｉｌａｎｄＷａｔｅｒＣ...  相似文献   

东北虎雌性生殖系统解剖     

刘玉堂  应璐  徐艳春  赵广英 《经济动物学报》2001,5(3):40-43

对 2例 1 999年 1 0月意外死亡的东北虎雌性生殖系统进行了解剖。雌性东北虎的卵巢被覆卵巢囊 ,随着年龄增长卵巢表面凸凹不平 ,呈脑的沟回状。输卵管位于卵巢游离缘外侧 ,呈半圆形围绕着卵巢 ,输卵管壶腹部弯曲、峡部直 ;子宫与输卵管分界明显 ;子宫角长度约相当于子宫体长度的 2倍 ;阴道呈背腹压扁的管状  相似文献   

图书馆工作人员应该转变的六个观念     

施小梅  何育华 《安徽农业大学学报》2002,(2):96-98

文章认为，要使图书馆工作适应新的技术革命和社会主义市场经济的需要，就必须转变观念。当前需要转变的观念有以下六个方面:图书馆由藏书中心变成文献资料中心、信息中心、智力开发中心;从关门办馆到开门办馆:从无偿服务到有偿服务:从独自办馆到馆际协作:从手工操作到运用现代技术手段:从与世无争到积极竞争。  相似文献   

近冰点温度贮藏草莓的研究     

郝利平  郝林 《果树学报》1993,(1)

草莓呼吸旺盛,果皮薄易受伤,难以贮藏。利用近冰点温度贮藏可以明显地抑制草莓的呼吸作用,结合小袋包装的气密性,改善了包装内部的气体组成,提高草莓果实周围的相对湿度并减少空气对流,从而抑制了草莓在贮藏过程中糖分、水分的损耗,减少了糖酸比值的下降。保持了一定的感观指标,可使草莓的贮藏时间延长。  相似文献   

靠接换砧矫治枳砧甜橙缺铁黄化研究     

韩为璨  张清华  李银国  王成秋  甘永康  王锡富  张方学  舒洪永 《中国南方果树》1993,(2)

靠接红桔、酸桔和枸头橙砧,矫治枳砧甜橙缺铁黄化有明显效果。其中,以枸头橙效果最好,以下依次为酸桔和红桔,靠接砧苗后树势逐年恢复,产量成倍增加,靠接红桔、酸桔和枸头橙分别比对照增产3.3、1.4和1.5倍;对提高果实含糖量、果汁率和降低含酸量亦有良好效果。  相似文献   

Porcine reproductive and respiratory syndrome virus: morphological, biochemical and serological characteristics of Quebec isolates associated with acute and chronic outbreaks of porcine reproductive and respiratory syndrome.   总被引：7，自引：2，他引：5       下载免费PDF全文

H Mardassi  R Athanassious  S Mounir    S Dea 《Canadian journal of veterinary research》1994,58(1):55-64

Cytolytic and noncytolytic strains of the porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in primary cultures of porcine alveolar macrophages (PAM) from lung homogenates of stillborn fetuses or blood samples of dyspneic piglets collected from Quebec pig farms having experienced acute or chronic outbreaks of PRRS. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. Intracytoplasmic enveloped viral particles that tended to accumulate into cytoplasmic vesicles were observed in the infected PAM; no budding was demonstrated at the level of the cytoplasmic membrane. The extracellular virions appeared as pleomorphic but mostly spherical enveloped particles, 50-72 nm in diameter (averaged diameter of 50 particles was 58.3 nm), with an isometric core about 25-30 nm. Buoyant density of the virus in CsCL density gradients was estimated to 1.18-1.20 g/mL. No hemagglutinating activity was demonstrated. Analysis of semipurified virions of isolate IAF-exp91 by radioimmunoprecipitation (RIPA) and Western immunoblotting experiments, using reference rabbit and porcine hyperimmune sera, revealed four major viral proteins, a predominant 15 kD N protein and three other proteins with predicted M(r_ of 19, 26 and 42 kD. Progeny viral particles produced in PRRSV-infected PAM in the presence of tunicamycin lacked the 42 kD protein, thus confirming its N-glycosylated nature. Immunoprecipitation experiments using the anti-ATCC-VR2332 MoAbs confirmed the close antigenic relationships between Quebec and American reference isolates of PRRSV.  相似文献   

星星草治理草原碱斑的效果与种植技术     

赵喜印  佟振宇 《草业科学》1992,9(5):61-62

  相似文献   

PEG诱导水分胁迫对柑桔幼苗细胞质膜透性及脯氨酸含量的影响   总被引：3，自引：0，他引：3  

张云贵  谢永红  吴学良  毛启才 《果树学报》1995,(Z1)

柑桔叶片相对含水量(RWC)随聚乙二醇(PEG)处理渗透势的降低而依次降低;叶片细胞质膜透性随PEG处理浓度的增加而依次增大;幼苗叶片累积脯氨酸的数量随水分胁迫时间和强度递增。  相似文献