泰国产方斑东风螺养殖群体遗传多样性的RAPD分析     

黄海  王永强  尹绍武  雷从改  冯永勤  陈国华  张本 《中国生态农业学报》2007,15(6):126-130

用随机扩增多态性DNA(RAPD)技术对泰国产方斑东风螺养殖群体的遗传多样性进行检测,从100个随机引物中筛选出21个引物对方斑东风螺的DNA进行扩增,结果表明:21个引物共检测到222条清晰且重复性好的条带,每个引物可扩增出4~16条带,分子量在200~2200bp之间,其中多态位点为156个,占70.27%;群体的Shannon多样性指数为0.2818,Nei基因多样性指数为0.2491;个体间最大遗传距离为0.291,最小遗传距离为0.066。通过与其他贝类遗传多样性的研究结果比较,可初步判断泰国产方斑东风螺养殖群体的遗传多样性比较丰富。  相似文献   

淮山品种"桂淮2号"的选育及栽培研究   总被引：6，自引：0，他引：6  

WEI Ben-hui  韦本辉  甘秀芹  韦威泰  唐荣华  宁秀呈  覃维治  黄永清 《广西农业生物科学》2005,24(1):48-50

淮山为薯蓣科薯蓣属多年生蔓性块茎类植物，是药食兼用的高效经济作物。通过优良单株筛选，结合群体观察以及植物学、生理学、产量和营养品质等相关研究，从广西地方种质资源中选育出淮山品种“桂淮2号”，并介绍其特征特性和田间肥水管理及病虫害防治等栽培技术要点。该品种具有淀粉含量高、矿质营养丰富等特点，可作为药材加工和菜用、粮用、饲用等。  相似文献   

In vitro screening and QTL analysis for drought tolerance in diploid potato     

A. M. Anithakumari  Oene Dolstra  Ben Vosman  Richard G. F. Visser  C. Gerard van der Linden 《Euphytica》2011,181(3):357-369

Drought stress is a major abiotic constraint limiting crop production worldwide. Screening for drought tolerance and the traits that enhance drought tolerance is not straightforward in large mapping populations. In this study, we investigated the possibility of screening a mapping population in vitro for PEG-induced water deficit stress and recovery potential. We have measured several shoot and root growth parameters or traits in the C × E diploid potato mapping population. Significant variation was observed for genotype-specific responses to water deficit and recovery potential. Genetic variation and heritability estimates were high to very high for the measured traits depending on growth conditions. In order to identify potato QTLs for drought tolerance and recovery potential an SNP marker-rich integrated linkage map was used. A total of 23 QTLs were detected under control, stress and recovery treatments explaining 10.3–22.4% of the variance for each phenotypic trait. Among these, 10 QTLs were located on chromosome 2. Three QTLs involved in the important trait root to shoot ratio were identified on linkage groups 2, 3 and 8. These loci explained together 41.1% of the variance for this trait, and may be breeding targets for stress tolerance and yield in the field as well. The SNP markers derived from EST sequences underlying these QTLs led to the identification of putative candidate genes for further study in potato. This study constitutes the first knowledge of in vitro screening of a mapping population for drought tolerance in potato.  相似文献   

安徽烟区土壤供钾特性的研究     

马友华  陈邦本  方明 《安徽农业大学学报》1992,(1)

用常规分析、钾的容量强度(Q/I)关系和电超滤(EUF)等法研究了安徽烟区黄红壤、黄棕壤和砂姜黑土的供钾特性。结果表明:粘粒矿物以高岭石、水云母为主的黄红壤有较高的供钾强度及速效钾数量,但土壤的供钾潜力及钾位缓冲容量PBC~k较低,固钾能力弱。粘粒矿物以水云母、蒙脱石为主的黄棕壤及以蒙脱石、水云母为主的砂姜黑土的供钾特性与黄红壤相反,其中砂姜黑土除固钾能力高于黄棕壤外,其他供钾指标略低于黄棕壤。土壤的供钾特性对烟草的吸钾量及钾肥肥效有直接影响。  相似文献   

Adaptive phenotypic plasticity in response to climate change in a wild bird population     

Charmantier A  McCleery RH  Cole LR  Perrins C  Kruuk LE  Sheldon BC 《Science (New York, N.Y.)》2008,320(5877):800-803

Rapid climate change has been implicated as a cause of evolution in poorly adapted populations. However, phenotypic plasticity provides the potential for organisms to respond rapidly and effectively to environmental change. Using a 47-year population study of the great tit (Parus major) in the United Kingdom, we show that individual adjustment of behavior in response to the environment has enabled the population to track a rapidly changing environment very closely. Individuals were markedly invariant in their response to environmental variation, suggesting that the current response may be fixed in this population. Phenotypic plasticity can thus play a central role in tracking environmental change; understanding the limits of plasticity is an important goal for future research.  相似文献   

When fear is near: threat imminence elicits prefrontal-periaqueductal gray shifts in humans     

Mobbs D  Petrovic P  Marchant JL  Hassabis D  Weiskopf N  Seymour B  Dolan RJ  Frith CD 《Science (New York, N.Y.)》2007,317(5841):1079-1083

Humans, like other animals, alter their behavior depending on whether a threat is close or distant. We investigated spatial imminence of threat by developing an active avoidance paradigm in which volunteers were pursued through a maze by a virtual predator endowed with an ability to chase, capture, and inflict pain. Using functional magnetic resonance imaging, we found that as the virtual predator grew closer, brain activity shifted from the ventromedial prefrontal cortex to the periaqueductal gray. This shift showed maximal expression when a high degree of pain was anticipated. Moreover, imminence-driven periaqueductal gray activity correlated with increased subjective degree of dread and decreased confidence of escape. Our findings cast light on the neural dynamics of threat anticipation and have implications for the neurobiology of human anxiety-related disorders.  相似文献   

老参地短期轮作技术体系的建立Ⅰ.人参西洋参轮作体系     

田义新  王本有  盛吉明  尹春梅  金志哲  杨继祥 《吉林农业大学学报》2002,24(3):46-49

老参地问题是制约参业发展的重大难题，笔者在总结前人关于老参地研究成果和经验的基础上，采取了测土施肥，有益微生物菌剂处理，酸碱度调整等土壤处理方法，成功地实现了老参地的短期再利用，建立了周期为1－3年的人参和西洋参的短期轮作体系。在处理后的老参地上种植西洋参，其长势，产量，病虫害及烧须情况，总皂苷含量等，与对照地新林土栽参无显著差异（P＞0．05）。并开展了中试规模的推广，试验结果显示，老参地短期轮作制是改造老参地的有效措施之一，该轮作技术方法应用简便，实用，可操作性强，轮作效果好。  相似文献   

意向性建构原理与读者理论     

陈本益 《湖南农业大学学报(自然科学版)》2002,(5)

现象学哲学的“意向性建构”原理的实质意义给与 :主体建构对象就是给与对象意义。这是讲求读者建构 (再创造 )文本意义一路文学理论的哲学基础  相似文献   

Long-Term Variable Rate Lime and Phosphorus Application for Piedmont No-Till Field Crops     

Weisz  Randy  Heiniger  Ron  White  Jeffrey G.  Knox  Ben  Reed  Lonnie 《Precision Agriculture》2003,4(3):311-330

Variable rate (VR) fertilizer application is a paradigm with potential to improve input efficiency and farm profitability. It is widely marketed by commercial applicators in the southeastern US. However, field studies comparing VR with traditional management have not demonstrated consistent, positive results. The objectives of this study were: (1) to determine the soil impact, crop response and economic potential of VR phosphorus (P) and lime application in a North Carolina Piedmont no-till field crop system using intensive soybean [Glycine max (L.) Merr.] production and (2) to economically evaluate alternatives to standard commercial grid soil sampling for directing VR P and lime. A 23-ha long-term no-till field in the SE Piedmont was divided into 0.4ha plots assigned to either VR or uniform P and lime application. Grid soil sampling and VR P and lime application were done prior to four crops over 3 years: full season soybean, wheat (Triticum aestivum L.)–double cropped soybean, and full season soybean. Soil test P, pH and crop yield response to VR P were inconsistent. Soil pH in areas with low pH initially did increase in response to VR lime, but it took two to three applications to bring all of these areas to the target pH. Once VR-liming raised initially low soil pH to levels close to target, yield of soybean, but not wheat, were up to 0.74Mgha^–1 higher than with uniform lime. Even with significantly higher soybean yields associated with VR lime, 3 years of grid sampling and VR application were not profitable compared to uniform application. The results indicated that VR lime could be profitable if the initial grid sampling data were used either for 2 consecutive years, or if it was used to restrict future grid sampling to specific areas requiring further VR lime.  相似文献   

Biosynthesis of the enediyne antitumor antibiotic C-1027     

Liu W  Christenson SD  Standage S  Shen B 《Science (New York, N.Y.)》2002,297(5584):1170-1173

C-1027 is a potent antitumor agent with a previously undescribed molecular architecture and mode of action. Cloning and characterization of the 85-kilobase C-1027 biosynthesis gene cluster from Streptomyces globisporus revealed (i) an iterative type I polyketide synthase that is distinct from any bacterial polyketide synthases known to date, (ii) a general polyketide pathway for the biosynthesis of both the 9- and 10-membered enediyne antibiotics, and (iii) a convergent biosynthetic strategy for the C-1027 chromophore from four building blocks. Manipulation of genes governing C-1027 biosynthesis allowed us to produce an enediyne compound in a predicted manner.  相似文献