干旱胁迫下八棱海棠中丙二醛及质膜透性的变化     

党云萍  李春霞 《北方园艺》2012,(21):33-35

以盆栽八棱海棠幼苗为试材,保持45%～50%土壤含水量进行中度干旱处理,研究干旱胁迫过程中丙二醛和质膜透性的动态变化与八棱海棠的抗旱关系。结果表明:八棱海棠在中等干旱胁迫下,叶片的质膜透性和丙二醛含量均随干旱胁迫时间的延长而增大,但质膜透性在前4d增加较慢,第5天开始明显增加;而丙二醛在前5d增加较慢,5d后增加较大。前期还能抵抗一定的干旱胁迫的强度,当超过一定强度时,细胞大量坏死,植物受到严重伤害甚至死亡。  相似文献   

百合种球采收及冷处理技术——以隆德县繁育的百合种球为例     

党亚平 《宁夏农林科技》2013,(11):26-26,37

介绍了冷凉地区繁育的百合种球的采收和冷处理技术,包括采收时间和标准、挖球、冷库消毒及摆放等.  相似文献   

松阿扁叶蜂生物学特性及防治技术研究   总被引：2，自引：0，他引：2  

党政武  周书剑 《陕西林业科技》2010,(3):44-46

松阿扁叶蜂是目前商洛市油松林重要食叶害虫,严重威胁油松林的生长发育。定点研究了其生物学特性、发生危害规律,开展了防治试验。松阿扁叶蜂在商洛林区1年发生1代,由幼虫越冬。其危害油松纯林重于混交林;立地条件较好的油松林分重于立地条件差的林分。喷施森得保粉剂（2.25 kg/hm2）、1.2%苦.烟乳油1 000~1 200倍液、3%高渗苯氧威乳油3 500~4 500倍液均可取得良好防效。  相似文献   

Deoxyribozyme inhibits the proliferation of human umbilical artery smooth muscle cells     

ZHOU Ming  WANG Wei  WANG Chong-quan  DANG Shu-yi  WANG Wei-min  WANG Xiao-xun 《园艺学报》2005,21(12):2334-2337

AIM: To observe the inhibition effects of 10-23 deoxyribozyme (DNAzyme) specific to human proliferating cell nuclear antigen (PCNA) gene on the proliferation of human umbilical artery smooth muscle cells (HUASMC) in vitro. METHODS: Using lipofectamine (Lip)-mediated method, the DNAzyme specific to PCNA was introduced into HUASMC. ［3H］-TdR incorporation was determined. The cell proliferation was examined by MTT assay and cell cycle was detected by flow cytometry. RESULTS: ［3H］-TdR incorporation in 1.0 μmol/L DNAzyme group was lower than that in control group (P<0.05) at 2 days after transfection. Compared with control group, the A value of MTT cholorimetric analysis decreased in 1.0 μmol/L DNAzyme group and antisense oligodeoxynucleotide (ASODN) group, at 2, 3 and 5 days after transfection significantly (P<0.01). The decrease in A value showed a dose-dependent manner within the experimental range at 5 days. After 2 days, the percentage of quiescence cells (G0/G1) was 73.8%, 54.7% and 41.1% in DNAzyme, ASODN and control groups, respectively. CONCLUSION: The DNAzyme specific to PCNA suppresses the proliferation of HUASMC effectively in vitro.  相似文献   

柔嫩艾美耳球虫表面抗原SAG2基因的克隆与表达   总被引：1，自引：0，他引：1  

简永利  蔡建平  于三科  覃宗华  林青  叶秀华  陈闻  党海斌 《畜牧与兽医》2006,38(6):10-13

根据生物信息学预测的基因序列设计引物,应用RT-PCR方法从柔嫩艾美耳球虫第二代裂殖子总RNA扩增获得了鸡柔嫩艾美耳球虫表面抗原2(surface antigen 2,SAG2)基因序列,将其与pGEM-T easy载体连接后转化E.coliDH5α,筛选阳性克隆,以带有限制酶切位点的特异性引物用PCR方法扩增不含SAG2 N端信号肽序列的ORF序列后克隆至表达载体pET-32 a(+),构建了重组表达质粒pET-32 a(+)-SAG2,并将其转化至E.coliBL21(DE3)。经IPTG诱导,获得了SAG2重组抗原在大肠杆菌的高效表达,重组蛋白的表达量约占菌体总蛋白的35%,融合蛋白的分子量约为47 ku。菌体经超声处理后进行SDS-PAGE分析表明,表达蛋白以包涵体的形式存在。  相似文献   

Herbicidal and fungicidal activities and identification of potential phytotoxins from Bidens pilosa L. var. radiata Scherff     

FARAH DEBA  TRAN DANG XUAN  MASAAKI YASUDA  SHINKICHI TAWATA 《Weed Biology and Management》2007,7(2):77-83

This study was carried out to examine the possibility of utilizing Bidens pilosa , a weed widely distributed in the subtropics and the tropics, for weed and plant fungus control. The extract of B. pilosa shows strong phytotoxic action against the growth of Raphanus sativus and Echinochloa crus-galli and antifungal activity against phytopathogens in bioassays. The extract of the roots exhibited plant growth inhibitory activity more than those of the leaves and stems. Fifteen phenolic compounds, including pyrocatechin, salicylic acid, p -vinylguaiacol, dimethoxyphenol, eugenol, 4-ethyl-1,2-benzenediol, iso- vanillin, 2-hydroxy-6-methylbenzaldehyde, vanillin, vanillic acid, p -hydroxybenzoic acid, protocatechuic acid, p -coumaric acid, ferulic acid, and caffeic acid were identified by gas chromatography–mass spectrometry. Caffeic acid was in the highest amount among the detected substances. Dimethoxyphenol and vanillic acid were found only in the roots of B. pilosa . Ferulic acid and p -hydroxybenzoic acid were higher in the roots than in the leaves and stems. These identified constituents might be responsible for the phytotoxic and fungitoxic activities of B. pilosa.  相似文献   

独山瓜馥木叶挥发油的主要成分及其抗肿瘤活性分析     

张贵源  龚莉莉  党荣敏 《贵州农业科学》2012,40(9):67-69

为了进一步深入研究独山瓜馥木的药理作用,挖掘其药用价值,采用水蒸气蒸馏法提取独山瓜馥木挥发油,运用GC-MS分析其主要成分。结果表明:独山瓜馥木主要成分为β-水芹烯(24.3%)、香叶烯-D(8.4%)、反式β-石竹烯(6.1%)和氧化石竹烯(4.5%),挥发油对K562、S-180和A549细胞株的IC50分别为22.5μg/mL,34.2μg/mL和64.4μg/mL;采用S180细胞株复制小鼠肿瘤模型,挥发油在剂量为80mg/kg、128mg/kg和204mg/kg时的抑瘤率分别为30.4%、49.6%和64.2%。  相似文献   

一种户用沼气池气密性检验方法     

郑庆卓  亓翠玲  党常英 《中国沼气》2007,25(5):45-45

气密性的检验在沼气池启用中起着关键性的作用,抗渗性能是否达到要求,关系到沼气池的质量和投入使用后的产气效益问题。目前,国家正大力实施沼气项目,数量多,任务重,传统的沼气池气密性检验方法费工、费时,且不易操作。作者在认真总结传统试压方法的基础上,经过多年的实践探索,  相似文献   

陕西省农业清洁生产的现状和对策     

党雪瑞  廖允成  王雯  范海阔 《安徽农业科学》2007,35(33):10820-10821

阐述了陕西省实施农业清洁生产的重要意义,并针对陕西省在实施农业清洁生产过程中存在的问题,提出了战略措施。  相似文献   

志丹县仁用杏栽培实用技术初探   总被引：1，自引：0，他引：1  

党彦林 《陕西林业科技》2007,(3):159-161

本文根据志丹县干旱少雨、霜冻频发的气候条件，结合近10a仁用杏生产实践，提出了从园地、品种选择，栽植时期，整形修剪，防霜保果和水肥管理等5个方面的实用生产技术，对志丹县及同类相近条件地区仁用杏发展具有参考价值。  相似文献