Long-term survival of a cow with cervical ectopia cordis     

Onda K  Sugiyama M  Niho K  Sato R  Arai S  Kaneko K  Ito S  Muto M  Suganuma T  Wakao Y  Wada Y 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(6):667-669

This study investigated the long-term survival of a calf with cervical ectopia cordis that grew normally, became pregnant, and calved normally. The cow showed normal cardiac function and absence of peripheral circulation abnormalities. This paper documents antemortem characteristics of the affected cow.  相似文献   

Detection of chromogranin A in the adrenal gland extracts of different animal species by an enzyme-linked immunosorbent assay using Thomsen-Friedenreich antigen-specific Amaranthus caudatus lectin     

Akiyoshi H  Sugii S  Nahid MA  Sone K  Tanaka T  Zheng C  Yijyun L  Aoki M  Takenaka S  Shimada T  Shimizu J  Kiyomiya K  Ohashi F 《Veterinary immunology and immunopathology》2011,144(3-4):255-258

The reactivity of different lectins with crude chromogranin A (CgA) obtained from different animals, namely, cow, horse, dog, pig, and dolphin, was examined to identify lectin(s) that would be useful as coating reagent(s) in a sandwich enzyme-linked immunosorbent assay (ELISA). Of the different lectins studied, the Amaranthus caudatus lectin (ACA), which is specific for the Thomsen-Friedenreich (T)-antigen (Galβ1-3GalNAc), was found to react with the CgA from different animals by western blotting. Purified rabbit anti-bovine CgA antibody was also found to cross-react with the crude CgA preparations. On the basis of these findings, a sandwich ELISA was developed with ACA as the coating reagent and anti-bovine CgA antibody as the probing antibody. Using this method, concentration-dependent curves ranging from 0.003 μg/mL to 25 μg/mL and from 0.02 μg/mL to 25 μg/mL were obtained for bovine CgA and canine CgA, respectively. Similarly, concentration-dependent curves were obtained for the equine, swine, and dolphin crude CgA extracts. Thus, ACA is concluded to be a valuable reagent for CgA detection in crude extracts from different animal species, and for CgA isolation/purification.  相似文献   

Nitrocapsanthin and nitrofucoxanthin, respective products of capsanthin and fucoxanthin reaction with peroxynitrite     

Tsuboi M  Etoh H  Kato K  Nakatugawa H  Kato H  Maejima Y  Matsumoto G  Mori H  Hosokawa M  Miyashita K  Tokuda H  Suzuki N  Maoka T 《Journal of agricultural and food chemistry》2011,59(19):10572-10578

The in vitro reactivity of capsanthin (1) and fucoxanthin (2) with peroxynitrite was investigated, and the reaction products produced by scavenging with peroxynitrite were analyzed. (14'Z)-Nitrocapsanthin (3) and 12-nitrocapsanthin (4) were isolated from the products of the reaction of capsanthin with peroxynitrite. Similarly, (14Z)-15-nitrofucoxanthin (5), (11Z)-11-nitrofucoxanthin (6), and (14Z,9'Z)-15-nitrofucoxanthin (7) were obtained from the reaction of peroxynitrite reaction with fucoxanthin. Capsanthin and fucoxanthin inhibited the nitration of tyrosine by peroxynitrite. Furthermore, nitrocapsanthins (3 and 4) and nitrofucoxanthins (5 and 6) exhibited an inhibitory effect on Epstein-Barr virus early antigen activation in Raji cells and an antiproliferative effect on human pancreatic carcinoma. Moreover, nitrocapsanthins (3 and 4) inhibited carcinogensis of mouse skin tumors initiated by 7,12-dimethylbenz[a]anthracene (DMBN).  相似文献   

Effect of potassium rate on growth,quality, chemical composition,and winter hardiness of perennial ryegrass     

Khorshid Razmjoo  Seiji Kaneko 《Journal of plant nutrition》2013,36(1):195-201

Seven potassium (K) rates (0, 50, 150, 350, 450, 550, and 650 kg K/ha) were used to determine the optimum rate of K for turf‐type perennial ryegrass during the winter based on turf growth, quality, and winter hardiness. Turf density, color, growth, and winter hardiness were increased as the rate of K application increased up to 350 kg K/ha. However, there were no beneficial effects of K application beyond that rate. Potassium content of the whole plant was 1.72% of the dry matter at the 350 kg K/ha rate. Calcium and Mg contents of the plant were reduced as the result of K application, and a reduction in growth and K content was detected at the higher K application rates. It is suggested that the optimum K rate for perennial ryegrass is about 350 kg K/ha under our experimental conditions in the winter, and that the K content should be about 1.72% of dry weight of whole plant.  相似文献   

Effect of fertility ratios on growth and turf quality of perennial ryegrass (Lolium prenne L.) in winter     

Khorshid Razmjoo  Seiji Kaneko 《Journal of plant nutrition》2013,36(8):1531-1538

Perennial ryegrass (Lolium prenne L.) has been introduced to Japan as a cool season turfgrass. In spite of relatively mild winter in most parts of the country, it still goes to winter dormancy. A well‐balanced fertility ratio and rate may prevent such dormancy without causing winter injury. Therefore, this experiment was designed to find a balanced rate and ratio among nitrogen (N), potassium (K), and phosphorus (P) fertilizers in order to prevent winter dormancy and promote growth during the winter in Japan. Yorktown 2 perennial ryegrass was used in this experiment. Nitrogen and K application rates were 50, 150, 250, 350, 450, 550, and 650 kg K/ha; whereas P rates were 50, 150, and 250 kg P/ha. These rates were applied in 30 different ratios. Growth, color, and density of the turf were estimated and used as the parameters for finding a suitable rate and ratio for these three major nutrients. Color, density, and growth were increased as the rate of N fertilizer increased, but there was no visual response to P or K rates. It is suggested that increases in color, density, and growth were function of N rates, whereas K and P rates maintained the cold hardiness of the turf when high N rates were applied under the conditions of this experiment. Fertility ratio and the rates of 450 kg N/ha, 250 kg K/ha, and 50 kg P/ha were sufficient to prevent dormancy, promote growth, and produce good quality turf throughout the winter. Thus, it is concluded that fertility ratios and rates are the main factors limiting growth and quality of perennial ryegrass in transitional zones similar to Japan.  相似文献   

Cloning and characterization of a NADP+-malic enzyme gene from Bradyrhizobium japonicum USDA110     

Suphawat Sinsuwongwat  Amane Kodera  Takakazu Kaneko  Satoshi Tabata  Mika Nomura  Shigeysuki Tajima 《Soil Science and Plant Nutrition》2013,59(5):711-717

Malic enzymes have been considered to play a key role in energy metabolism for nitrogenase reaction in bacteroids. To elucidate the physiological role of the malic enzymes in Bradyrhizobium japonicum bacteroids, a putative malic enzyme gene Bjtme1 was cloned by polymerase chain reaction (PCR) using degenerated primers from conserved regions of the protein sequences of bacterial malic enzymes and draft sequence data of the Bradyrhizobium japonicum USDA110 genome sequence project. To confirm the characteristics of the Bjtme1 gene, the protein encoded by this gene was over-expressed using a pET32a(+) system and it exhibited a NADP⁺-malic enzyme (EC 1.1.1.40) activity, indicating that Bjtme1 was the gene of the NADP⁺-malic enzyme. This is the first report on the cloning and characterization of the NADP⁺-malic enzyme gene from B. japonicum, and the gene structure was compared with that of NADP⁺-malic enzyme genes of other rhizobia.  相似文献   

Shoot control of nodulation is modified by the root in the supernodulating soybean mutant En6500 and its wild-type parent cultivar Enrei     

Hideo Hamaguchi  Makie Kokubun  Shoichiro Akao 《Soil Science and Plant Nutrition》2013,59(4):771-774

Abstract

Based on grafting studies, both supernodulating (Carroll et al. 1985a, b) and hypernodulating (Gremaud and Harper 1989) soybean ([itGlycine max} L. Merr.) phenotypes were reported to be under the control of shoot factors (Delves et al. 1986, 1987; Cho and Harper 1991). Recently Akao and Kouchi (1992) have isolated a new supernodulating mutant (En6500) from ethyl methane sulfonate (EMS)-treated Enrei, a cultivar which is widely grown in the central districts of Japan. This mutant has been shown to produce several fold as many nodules as its wild-type parent cultivar when grown at a low concentration (0.5 mol m^-3) of nitrate. Moreover, it exhibited a continuous increase in the nodule number with the increasing nitrate concentration, even at 15 mol m^-3 (Francisco et al. 1992), in contrast to the similar mutant nts382 in which the nodulation decreased even at the relatively low nitrate level of 5.5 mol m^-3 (Carroll et al. 1985a). In this study we conducted grafting experiments to determine which plant part controls the supernodulation of En6500.  相似文献   

On the absorption of water and salt by the rice Plant root system,Especially mutual relationship between young and old roots     

Hideo Okajima 《Soil Science and Plant Nutrition》2013,59(3):104-114

GILE et al (2) grew rice or corn with one half of the roots in a solution lacking in one element and the other half in a complete solution, and found out that these plants could absorb approximately three-fourths as much ion as the normal plants. Therefore, when a portion of the root is supplied with an ion, ion absorption by the roots may be greater than normal plants. For example, in their experiments, nitrogen absorbed per gram of roots, by the partial-nitrogen p!ants amounted to 1.45 times as much as the normal plants.  相似文献   

Time course study of aluminum-induced callose formation in barley roots as observed by digital microscopy and low-vacuum scanning electron microscopy     

Maiko Kaneko  Etsuro Yoshimura  Naoko K. Nishizawa  Satoshi Mori 《Soil Science and Plant Nutrition》2013,59(3):701-712

To clarify the mechanism(s) involved in the short-term inhibition of root elongation by AI, we monitored the morphological changes of barley roots by digital microscopy. Within 30 min after exposure to 37 µM AI, the surface of the root epidermis in the region of a distance of 1.5 mm from the root tip became rough and began to show signs of damage. After 38 min, callose was rapidly excreted from the junction between the root cap and the root epidermis, and formed a spherical lump approximately 60 µm in diameter. The fine structure of the callose deposits on the root surface was analyzed by low-vacuum scanning electron microscopy. After 50 min, there was a significant increase in the callose contents in the distal 0.6 mm part. At the same time, root elongation stopped completely. Fluorescence staining indicated that callose was localized on the surface of the cell elongation area (the elongation zone of primary roots and root hairs), but not on the surface of the meristem. The root growth reduction associated with AI treatment may be due to the use of sugar substrates for callose formation instead of cellulose formation.  相似文献   

Fate of nitrogen derived from 15N-labeled plant residues and composts in rice-planted paddy soil     

Hideo Ueno  Shigekazu Yamamuro 《Soil Science and Plant Nutrition》2013,59(4):747-754

Pot experiments that lasted for 3 y were conducted to investigate the dynamics of nitrogen derived from plant residues (rice root, hull, straw, corn root, and rapeseed pod-wall), and composts (rice straw compost, cattle manure compost, and cattle manure sawdust compost), which were labeled with ¹⁵N. The rates of nitrogen uptake by rice (=N efficiency), denitrification, and immobilization derived from the organic materials incorporated before the first year of cultivation were investigated throughout 3 y of cultivation. At the end of the first year of cultivation, relatively high rates of N efficiency were obtained for rapeseed pod-wall (24.6%), rice straw (19.1%), and rice hull (18.6%), while corn root and cattle manure sawdust compost displayed a noticeably high denitrification rate. Corn root, cattle manure sawdust compost, rice hull, and rapeseed pod-wall exhibited remarkably high N mineralization rates ranging from 60 to 75% of the organic materials N applied. Cumulative rates of N efficiencies from the organic materials applied before the first year of cultivation fitted well to a first-order kinetic model and their asymptotes were compared among the organic materials. The asymptotic rates of N efficiency tended to depend on the rates at the end of the first year of cultivation.  相似文献