不同环境下作物基因型值和杂种优势的分析方法   总被引：8，自引：0，他引：8  

吴吉祥  朱军 《浙江大学学报(农业与生命科学版)》1994,(6)

根据加性－显性与环境互作的遗传模型，提出了利用多个环境的杂交亲本和Ｆ＿１的资料，采用ＡＵＰ法预测作物杂种后代在不同环境中的基因型值和杂种优势的分析方法。作为分析实例，本文对１９９２年和１９９３年陆地棉（ＧｏｓｓｙｐｉｕｍｈｉｒｓｕｔｕｍＬ．）杂交亲本（Ｐ）、Ｆ＿１皮棉产量和衣分的两年资料进行了分析。  相似文献   

兔病毒性出血症病毒高免血清的制备与应用效果     

张冰  张丹  李军 《动物医学进展》2002,23(5):89-90

以兔病毒性出血症病毒组织灭活疫苗免疫家兔，经五次免疫之后，抗体效价可达12log2以上，采集兔血清，以此治疗攻毒兔，结果大部分兔都存活，临床应用效果表明：以此血清治疗自然感染兔病毒性出血症病毒的发病兔，治愈率达80％以上。  相似文献   

Investigation of Cervical Patency and Uterine Appearance in Domestic Cats by Fluoroscopy and Scintigraphy     

K Chatdarong  N Kampa  E Axnr  C Linde‐Forsberg 《Reproduction in domestic animals》2002,37(5):275-281

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid‐oestrus, late oestrus and interoestrus or a radiopharmaceutical (^99mTc‐HSA) during mid‐ and interoestrus in a non‐ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid‐oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol‐17β concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid‐oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral‐shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.  相似文献   

河南省食用菌科技推广存在的问题及对策   总被引：1，自引：0，他引：1  

侯军  段春燕  杜爱玲  谢清华  吴祖峰 《河南科技大学学报(农学版)》2001,21(3):184-185

综合分析了河南省食有菌科技推广中存在的生产经营缺乏宏观引导，科技推广存在自发性和盲目性，体制不协调和食用菌生产本身的不可控因素较多等问题，提出了加强宏观引导，微观调控，建立健全食用菌行业管理，食用菌龙头企业建设，开发建设规模生产基地等具体措施，为食用菌的产业化大发展提供了参考依据。  相似文献   

Reproductive roles predict sexual dimorphism in internal and external morphology of lake whitefish,Coregonus clupeaformis     

S. J. Casselman  A. I. Schulte‐Hostedde 《Ecology of Freshwater Fish》2004,13(3):217-222

Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs.  相似文献   

重组逆转录病毒载体的包装   总被引：1，自引：1，他引：0  

王凤阳  扈荣良  张茂林  赵君  涂长春  殷震 《中国兽医学报》2001,21(2):128-130

将3.7kb的LacZ基因片段克隆到含标志基因Neo的逆转录病毒载体，pLXSN与pLNCX的多克隆位点，构建了含有LacZ基因的重组逆转录病毒载体pLLSN和pLNCL.在测定了G418对饥装细胞系PA317的最小致剂量后（最小致死剂量为0.3g/L），通过脂质体Lipofectin与磷酸钙2种介质，分别将2种重组逆转录病毒载体导入包装细胞系PA317进行包装，并以0.3g/L，的G418进行筛选，得到多个G418抗性克隆，经扩大培养，传代，分别得到包装pLLSN与pLNCL的2株阳性细胞，电镜下可见阳性细胞的培养上清中具有逆转录病毒形态特征的成熟病毒粒子，本0研究为运用逆转录病毒载体系统，解决转角因效率低的问题奠定了基础。  相似文献   

如何加强市场经济条件下工程造价管理     

王正贵  孙倩  何军 《森林工程》2001,17(1):36-37

本文根据目前工程造价管理的实践，指出了存在的问题，并提出了如何加强市场经济条件下建设工程造价管理的四点具体意见，很有现实意义和指导意义。  相似文献   

PCR扩增TK基因检测鸡传染性喉气管炎病毒的研究   总被引：3，自引：0，他引：3  

彭大新  甘军纪  高崧  吴艳涛  刘金彪 《动物医学进展》2001,22(3):59-61

根据已发表序列设计一对包含鸡传染性喉气管炎病毒（ILTV）TK基因全长核苷酸的1259bp引物，对2株ILTV强毒和1株ILTV疫苗毒进行PCR扩增，均能扩增出预期大小的目的片段，酶切分析证实了目的片段的特异性，而其它禽病原体的扩增均为阴性。PCR检测ILTV DNA的最小检测量为75pg。此方法检测人工接种鸡的气管棉拭样品，均能检测到ILTV，因此可用于临诊样品鸡传染性喉气管炎病的检测和诊断。  相似文献   

刺槐种群生物量动态研究     

毕君  王振亮  黄则舟  赵志诚 《河北林果研究》1994,(2)

研究了河北省太行山刺槐种群乔木层生物量、各组分量及种群密度的动态规律。结果表明，刺槐种群自人工造林伊始，其种群总生物量、各组分量、种群密度（断面积表示）即表现出速增性，约至１２ａ生达到峰值，基本符合Ｌｏｇｉｓｔｉｃ规律。此后随个体间竞争的加剧，自疏作用加强，种群呈不稳定状态，种群密度和生物量下降，由一个同龄纯林逐渐趋向于具有多代个体年龄结构稳定的群体，并使种群生物量回复到一个相对稳定水平。本文还建立了生物量及各组分量与种群年龄、密度的相关模型，及Ｗ～ｆ（Ａ，Ｇ，Ｈ）多元线性回归议程。  相似文献   

刺槐单株生物量动态研究   总被引：3，自引：0，他引：3  

毕君  黄则舟  王振亮 《河北林果研究》1993,(4)

本文依据420株刺槐生物量及23株树干解析资料,应用灰色Verhulst模型,对太行山坡地刺槐林单株干、枝、叶、根生物量动态进行了研究,分别建立了预测模型,预测了各器官生长的速生期和停止生长林龄,为实现刺槐林的多目标经营提供决策依据。  相似文献