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51.
Partho Pratim Debnath Jerome Delamare-Deboutteville Mona Dverdal Jansen Kornsunee Phiwsaiya Afsana Dalia Md. Abir Hasan Saengchan Senapin Chadag Vishnumurthy Mohan Ha Thanh Dong Channarong Rodkhum 《Journal of fish diseases》2020,43(11):1381-1389
Tilapia lake virus (TiLV) is an emerging pathogen in aquaculture, reportedly affecting farmed tilapia in 16 countries across multiple continents. Following an early warning in 2017 that TiLV might be widespread, we executed a surveillance programme on tilapia grow-out farms and hatcheries from 10 districts of Bangladesh in 2017 and 2019. Among farms experiencing unusual mortality, eight out of 11 farms tested positive for TiLV in 2017, and two out of seven tested positive in 2019. Investigation of asymptomatic broodstock collected from 16 tilapia hatcheries revealed that six hatcheries tested positive for TiLV. Representative samples subjected to histopathology confirmed pathognomonic lesions of syncytial hepatitis. We recovered three complete genomes of TiLV from infected fish, one from 2017 and two from 2019. Phylogenetic analyses based on both the concatenated coding sequences of 10 segments and only segment 1 consistently revealed that Bangladeshi TiLV isolates formed a unique cluster within Thai clade, suggesting a close genetic relation. In summary, this study revealed the circulation of TiLV in 10 farms and six hatcheries located in eight districts of Bangladesh. We recommend continuing TiLV-targeted surveillance efforts to identify contaminated sources to minimize the countrywide spread and severity of TiLV infection. 相似文献
52.
Anupam Tripathi Chandra Mohan Singh Mukul Kumar Shalini Purwar Anuj Mishra Dharmendra Kumar Awnindra Kumar Singh Sujit Kumar Sanjay Singh Narendra Pratap Singh 《Plant Breeding》2023,142(5):668-681
Black gram is one of the most important short duration grain legume, which contributes significantly towards nutritional security and environmental sustainability. The virus specific primers confirms the presence of mungbean yellow mosaic India virus (MYMIV) in representative samples. A total of 27 cultivated and two wild species were found as highly resistant (HR) to MYMIV and validated through molecular markers. The start codon target (SCoT) markers analysis revealed that the SCoT loci, namely, SCoT-4 (2200 bp), SCot-9 (1150/ 1200 bp), SCoT-15 (1150/1100 bp), SCoT-16 (700 bp), SCoT-24 (2500 bp), SCoT-25 (700 bp), SCoT-33 (900/1000 bp), and SCoT-34 (600 bp), were found unique, able to distinguish HR and highly susceptible (HS) genotypes. Biochemical characterization and gene expression profiling revealed the higher expression of antioxidants and R-genes just after pathogen inoculation indicated the activation of defence mechanism in both cultivated and its wild relatives, which modulates the resistant responses in cultivated and wild accessions. These information will be really helpful in accelerating resistance breeding in black gram. 相似文献
53.
A. Josephrajkumar Chandrika Mohan J. Poorani Merin Babu Daliyamol V. Krishnakumar Vinayaka Hegde P. Chowdappa 《Phytoparasitica》2018,46(1):57-61
Scavenging action of Leiochrinus nilgirianus Kaszab (Coleoptera: Tenebrionidae) on sooty mould deposition on coconut palms due to heavy honeydew production by the invasive rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae), is reported for the first time from Kerala, India. Adult beetles, averaging 2.07?±?0.9 / leaflet, with a maximum of five beetles per leaflet, and immature stages were generally confined to the abaxial of the palm leaves during daytime, but were found feeding on sooty mould during morning hours in damp conditions. The specially aided adaptive leg features of L. nilgirianus with bristle-like hairs on the undersurface of tarsomeres probably aid in proper adhesion on sooty mould laden plant surface and well developed tarsal claws that perhaps assist swift movement on fungus-laden leaflets. On an average one adult beetle could clear 1–2 mm2 sooty mould laden area in a period of one minute. In situ habitat conservation of L. nilgirianus would help to reduce the sooty mould at no cost in the most natural and eco-friendly manner avoiding chemical management options and other expensive methods. 相似文献
54.
55.
Identification and marker‐assisted introgression of QTL conferring resistance to bacterial leaf blight in cowpea (Vigna unguiculata (L.) Walp.)
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Hebse Bhojappa Dinesh Hirenallur Chandappa Lohithaswa Kannalli Paramashivaiah Viswanatha Poonam Singh Annabathula Mohan Rao 《Plant Breeding》2016,135(4):506-512
Bacterial leaf blight (BLB), caused by Xanthomonas axonopodis pv. vignicola (Xav), is widespread in major cowpea [Vigna unguiculata (L.) Walp.] growing regions of the world. Considering the resource poor nature of cowpea farmers, development and introduction of cultivars resistant to the disease is the best option. Identification of DNA markers and marker‐assisted selection will increase precision of breeding for resistance to diseases like bacterial leaf blight. Hence, an attempt was made to detect QTL for resistance to BLB using 194 F2 : 3 progeny derived from the cross ‘C‐152’ (susceptible parent) × ‘V‐16’ (resistant parent). These progeny were screened for resistance to bacterial blight by the leaf inoculation method. Platykurtic distribution of per cent disease index scores indicated quantitative inheritance of resistance to bacterial leaf blight. A genetic map with 96 markers (79 SSR and 17 CISP) constructed from the 194 F2 individuals was used to perform QTL analysis. Out of three major QTL identified, one was on LG 8 (qtlblb‐1) and two on LG 11 (qtlblb‐2 and qtlblb‐3). The PCR product generated by the primer VuMt337 encoded for RIN2‐like mRNA that positively regulate RPM1‐ and RPS2‐dependent hypersensitive response. The QTL qtlblb‐1 explained 30.58% phenotypic variation followed by qtlblb‐2 and qtlblb‐3 with 10.77% and 10.63%, respectively. The major QTL region on LG 8 was introgressed from cultivar V‐16 into the bacterial leaf blight susceptible variety C‐152 through marker‐assisted backcrossing (MABC). 相似文献
56.
Yoshiaki HAYASHI Bhim B. THAPA Mohan P. SHARMA Maheshwor SAPKOTA Hajime KUMAGAI 《Animal Science Journal》2009,80(4):418-427
To identify the effects of whole crop maize silage (MS) as a substitute for rice straw (RS) on feed intake and milk production of mid-late lactating buffalo and cattle in Tarai, Nepal, eight Murrah and eight Jersey-Hariana were fed the basal diet, RS ( ad libitum ) with concentrate (0.68% of bodyweight [BW] on a dry matter [DM] basis). A 4 × 4 Latin square design experiment was conducted in each animal species with graded levels of MS substitution for RS (0%, T1; 33%, T2; 67%, T3 and 100%, T4). The MS had higher digestibility and total digestible nutrient (TDN) than RS. The DM intake per BW of the both species was highest in T3. The substitution of MS for RS increased the crude protein intake and the TDN intake in the both species. Although the buffalo showed the highest milking performance in T4, the cattle showed no significant differences in their milking performance among the treatments. The substitution of MS for RS improved the feed intake and milk production in the buffalo. On the other hand, the milk yield was not raised in the cattle, though the feed intake was increased by the substitution. 相似文献
57.
Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine 总被引:1,自引:1,他引:0
Bandyopadhyay S Sasmal D Dutta TK Ghosh MK Sarkar M Sasmal NK Bhattacharya M 《Tropical animal health and production》2009,41(4):587-592
The present study was carried out to explore the seroprevalence of brucellosis in yaks of North-Eastern hilly yak tracts of
Arunachal Pradesh, India. Of 374 animals tested, 23.79, 21.11 and 18.98% were found positive for brucellosis using avidin-biotin
ELISA (AB-ELISA), Rose-Bengal plate test (RBPT) and standard tube-agglutination test (STAT), respectively. The relative sensitivity
and specificity for STAT were 79.77 and 100%, respectively and the same for RBPT were 88.76 and 100%, respectively in comparison
to AB-ELISA. The alarming prevalence as recorded was highest among the yak cows (31.42%) followed by heifers (23.85%) and
bulls (8.88%). The immune response in yaks following standard dose of calfhood vaccination with Brucella abortus strain 19 vaccine showed that protective antibody level persisted up to 210 days. This is the first report from India on
prevalence of brucellosis and immunization with B abortus strain 19 vaccine in yaks. The present investigation would be a valuable guideline for future control measure and eradication
programme of brucellosis in yaks. 相似文献
58.
Maddur MS Mohan MS Gajendragad MR Kishore S Gopalakrishna S Singh N 《Veterinary research communications》2009,33(2):103-109
Humoral and mucosal (secretory antibody)immune response to FMDV type Asia 1 in cattle was analyzed after vaccination and infection
using virus neutralizing test (VNT). Vaccination (1/16th the usual dose) failed to protect cattle from generalized clinical
disease following experimental FMDV Asia 1 infection. Our results showed that infection induced higher and prolonged serum
antibody titres indicating antigen mass is important for optimal immune response. Experimental FMDV infection induced significant
secretory antibody (mucosal) response in cattle. Though, there was no difference in the serum antibody response between the
cattle that developed generalized infection (unprotected) and those with only localized infection (protected), secretory antibody
response differed, wherein the unprotected cattle had higher secretory response than protected cattle. Thus, FMDV Asia 1 infection
stimulates a similar serum antibody response and a unique secretory antibody response among the infected cattle.
An erratum to this article can be found at 相似文献
59.
60.
Kali Krishna Hazra Probir Kumar Ghosh Madasur Subbabhat Venkatesh Chaitanya Prasad Nath Narendra Kumar Mohan Singh 《Archives of Agronomy and Soil Science》2018,64(12):1690-1704
Long-term effect of mungbean inclusion in lowland rice-wheat and upland maize-wheat systems on soil carbon (C) pools, particulate organic C (POC), and C-stabilization was envisaged in organic, inorganic and without nutrient management practices. In both lowland and upland systems, mungbean inclusion increased very-labile C (Cfrac1) and labile C (Cfrac2) in surface soil (0–0.2 m). Mungbean inclusion in cereal-cereal cropping systems improved POC, being higher in lowland (107.4%). Lowland rice-based system had higher passive C-pool (11.1 Mg C ha?1) over upland maize-based system (6.6 Mg C ha?1) indicating that rice ecology facilitates the stabilization of passive C-pool, which has longer persistence in soil. Organic nutrient management (farmyard manure + full crop residue + biofertilizers) increased Cfrac1 and carbon management index (CMI) over inorganic treatment. In surface soil, higher CMI values were evident in mungbean included cropping systems in both lowland and upland conditions. Mungbean inclusion increased grain yield of cereal crops, and yield improvement followed the order of maize (23.7–31.3%) > rice (16.9–27.0%) > wheat (lowland 7.0–10.7%; upland 5.4–16.6%). Thus, the inclusion of summer mungbean in cereal-cereal cropping systems could be a long-term strategy to enrich soil organic C and to ensure sustainability of cereal-cereal cropping systems. 相似文献