PRRSV弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫效果观察     

金光明  王珏  宁康建  吕锦芳  李升和  应如海 《勤云标准版测试》2005,(3)

应用组织学方法比较观察了猪繁殖与呼吸综合征病毒(PRRSV)弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫的效果。试验选用5月龄长白×大约克夏二元杂交母猪18头。PRRSV弱毒细胞疫苗和灭活疫苗通过生殖道黏膜免疫接种。结果表明PRRSV弱毒细胞疫苗生殖道黏膜接种组母猪的子宫黏膜上皮内淋巴细胞数较灭活疫苗生殖道黏膜免疫接种组和对照组母猪显著减少(P<0.01);而且子宫黏膜上皮变矮变薄,变得有些不规则。灭活疫苗生殖道黏膜接种组母猪子宫黏膜上皮内淋巴细胞数与对照组母猪间差异不显著(P>0.05)。本试验结果显示PRRSV弱毒细胞疫苗经生殖道黏膜免疫接种则对生殖道黏膜上皮内淋巴细胞产生抑制作用。说明PRRSV弱毒细胞疫苗和灭活疫苗经生殖道黏膜免疫效果不理想  相似文献   

提高半湿润地区杂交玉米种子发芽率的有效措施     

宁乐怡 《种子世界》2005,(8):47-47

清水县位于甘肃省东南部,介于34°32′～34°58′N、105°45′～106°30′E之间,属陇中南部半湿润区,年降水量662mm左右,降水主要集中在7～9月份,占年降水量的55%.清水县城关川区中晚成熟玉米品种的生长期是从4月下旬后期到9月下旬后期,大致148d左右.  相似文献   

Development of Chinese Near-isogenic Line of Rice and Their Differentiating Ability of Pathogenic Races of Blast Fungus     

LING Zhong-zhuan  T.Mew  WANG Jiu-lin  LEI Cai-ling  HUANG Ning 《中国农业科学(英文版)》2001,(1):50-56

The rice( Oryza L. ) variety, Lijiangxintuanheigu(LTH), is a universally susceptible variety. A set of near-isogenic lines (NILs) of rice which has single resistance gene for each line was developed by use of LTH as recurrent parent and Kusabue,Tsuyuake, K1, PiNo. 4, K60 and BL1, which are from Kiyosawa‘s differential varieties(KDV), as donor parents. Differentiating ability of these NILs to Philippine isolates was compared with that of their donor parents, KDV and IRRI‘ s NILs. The results of comparative studies were as follows: (1) Differentiating ability of Chinese NILs to Philippine isolates was much stronger than that of their donor parents and that of KDV. (2) Chinese NILs had similar ability to differentiate races to IRRI‘s NILs or higher differentiating ability than them. But IRRI‘s NILs almost had no differentiating ability to isolates from japonica rice-growing regions. They can not be used to differentiate races of isolates from japonica ricegrowing regions. Whereas, Chinese NILs can be used to differentiate races of isolates from both japonica ricegrowing regions and indica rice-growing regions. It was concluded that the set of Chinese NILs can be used as international differentials.  相似文献   

Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.     

Ning De-juan Hou Pei-chen Hu Zan-min Shen Xin Chen Shao-liang College of Biological Sciences  Biotechnology  Beijing Forestry University  Beijing  P. R. China Institute of Genetics  Developmental Biology  Chinese Academy of Sciences  Beijing  P. R. China 《中国林学(英文版)》2006,8(4):15-19

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.  相似文献   

鸡大肠杆菌病中草药佐剂多价灭活苗的研制——Ⅰ、疫苗生产工艺   总被引：2，自引：1，他引：1  

王文成  卫广森  王卓  尹广东  宣华  李素 《中国兽药杂志》2001,35(6):18-21

采用鸡大肠杆菌多发病型菌株以及地方病型菌株作为制苗用菌种，保证了疫苗既具有一定的广谱性，又具有一定的特异性；采用液体通气培养方法，在一定程度上提高了菌数；采用中草药蜂胶作为佐剂不但可有效地提高机体的免疫功能，同时兼具有治疗，保健和促生长作用，另外蜂胶还可以增大抗原表面积，延长抗原在组织的贮存时间，可持久地释放抗原，保证了机体持续产生高效价抗体。  相似文献   

新疆湿地保护管理学习考察体会     

冯宁  周灵国 《陕西林业》2009,(2):28-28

为提高我省湿地保护管理工作者的业务水平，应广大湿地保护管理人员的要求，根据省厅2008年工作计划的安排，组织了这次湿地保护管理考察学习。通过考察学习，使参与湿地保护管理的工作人员对湿地有了充分的认识，学习新疆林业厅在各湿地保护区工作中的经验，将学到的经验在陕西各地进行推广，推动湿地保护管理工作迈上新的台阶。  相似文献   

哺乳动物附植前胚胎的基因表达调控   总被引：4，自引：0，他引：4  

姚宁  曾申明 《中国畜牧杂志》2006,42(1):64-67

哺乳动物胚胎附植前期包括:合子的形成,胚胎基因组的激活和细胞分化的开始。在这个时期,发育由母源物质控制转为合子基因控制,在此过程,同时形成染色质介导的转录抑制时期,要解除抑制必须经过胚胎基因组的激活。通过对体内、外附植前胚胎的mRNA的表达特点以及它们与成功发育联系的研究,可以筛选出最佳的体外培养条件,设计最佳的核移植方案。  相似文献   

江玉林：“绿色通道建设”漫谈     

王志强  吕宁 《绿色中国(综合版)》2005,(11):55-57

我国的高速公路到2002年总里程仅次于美国，名列世界第二。据规划，到2020年，我国基本形成国家高速公路网。高速公路的跨越式发展，必然会增加生态系统的压力。这不由得不使我们对我国高速公路建设中的生态问题仔细审视。江玉林博士，作为交通部科学研究院副总工，交通可持续发展中心主任（兼），研究员，国际水土保持专家。提出了她的看法和认识。  相似文献   

离子选择电极法测定NBPG与银、镉、汞的配合物组成和稳定常数     

孙桂珍  阎早婴  宁开桂 《河北北方学院学报(自然科学版)》1995,(1)

本文用离子选择电极法测定了N,N—二甲基膦酸基甘氨酸(NBPG)与银、镉、汞的配合物组成和稳定常数。结果表明,NBPG与Ag~ 、Cd~(2 )、Hg~(2 )均生成组成为1:1的多齿质子型配合物。其lgK_稳值分别为:AgL5.28、CdH_3L3.63、CdH_2L5.16、CdHL7.30、CdL11.56、HgH_3L6.06、HgH_2L7.55、HgHL8.24、HgL12.58。  相似文献   

‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献