Changes in fertility of rainbow trout eggs retained in coelom   总被引：1，自引：0，他引：1  

TERUO  AZUMA  HIROMI  OHTA  SABURO  ODA  KOJI  MUTO  TAKASHI  YADA  TATSUYA  UNUMA 《Fisheries Science》2003,69(1):131-136

ABSTRACT:    Effects of prolonged retention time of ovulated eggs in the parental coelom on fertilization success were studied in rainbow trout Oncorhynchus mykiss using cryopreserved sperm with a uniform fertilizing ability. Proportions of successful fertilization, eyed eggs and hatched alevins were examined at different time periods up to a retention time of 14 days beyond the ordinary stripping time, and were compared with eggs incubated in artificial coelomic fluids (ACF). Eggs that were retained longer in the coelom showed gradual decreases in all the proportions of successful fertilization, eyed eggs and hatched alevins. The progress of cleavage after fertilization slowed with prolonged retention times. Eggs incubated in ACF lost their fertilizing ability much sooner than those retained in the coelom. The hatching rate of eggs retained for 2 weeks in coelom was 36%, while it was 1% in those eggs incubated for 4 days in ACF. Thus, eggs retained in the coelom showed higher fertilization success than those incubated in ACF.  相似文献   

Mediastinal basaloid carcinoma arising from thymic cysts in two dogs     

Yuto SANO  Kanae SEKI  Kenjirou MIYOSHI  Toshikazu SAKAI  Tsuyoshi KADOSAWA  Kazuya MATSUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):876

Mediastinal masses in dogs were diagnosed as basaloid carcinoma associated with multiple thymic cysts (MTCs). The masses were composed of MTCs and proliferating intracystic neoplastic basaloid cells, which immunohistochemically diffusely expressed p63 and cytokeratin 19. A gradual transition from the basal cell layers lining the cysts walls to the neoplastic cells was seen, and it was indicated that the neoplastic cells had originated from the basal cell layers of the cysts. To the best of our knowledge, this is the first report of basaloid carcinoma occurring in the mediastinal cavity in dogs. Although these tumors were demonstrated to be rare origins, basaloid carcinoma should be included in the differential diagnoses for canine mediastinal tumors.  相似文献   

THE CATEGORIES OF ALUMINIUM- AND IRON-HUMUS COMPLEXES IN ANDO SOILS DETERMINED BY SELECTIVE DISSOLUTION   总被引：2，自引：0，他引：2  

KOJI WADA  TERUO HIGASHI 《European Journal of Soil Science》1976,27(3):357-368

Thirty-three samples of Ando soils were extracted by sodium pyrophosphate (O.IM; pH 10) and by dithionite-citrate. The Al, Fe, and C contents of these two extracts offer a means of differentiating the status of humus in relation to Al and Fe in the different horizons. The humus that forms at first in the Al horizon has a very low complexing ability for Al and Fe and little is dissolved by pyrophosphate. The humus evolves with time or pedogenesis into forms that complex Al and Fe released from volcanic ash by weathering, and which are dissolved by pyrophosphate. In the old horizons, the humus further reacts with additional Al and Fe, some of which may be present as hydrous oxides or allophane-like constituents, allophane and imogolite. This reaction probably makes the humus less soluble in pyrophosphate.  相似文献   

Sperm whales associated with Gulf Stream features off the north-eastern USA shelf   总被引：1，自引：0，他引：1  

GORDON T. WARING  CAROL P. FAIRFIELD  CYNTHIA M. RUHSAM  MARGARET SANO 《Fisheries Oceanography》1993,2(2):101-105

Marine mammal surveys were conducted during sum Mer 1990 and 1991 in shelf-edge and off-shelf waters between Cape Hatteras and Georges Bank. Sperm whales were the most frequently sighted large whale in both years, constituting 27.9% and 12.7% of total sightings in 1990 and 1991, respectively. Sighting rates of sperm whales that were within a 9.3 km buffer (5 n.m.) of the Gulf Stream north wall were not significantly different (x²= 2.86, P > 0.05) from those within the Gulf Stream during 1990. In 1991, however, significantly more (x²= 51.0, P < 0.05) sightings were associated with shelf-edge areas where warm core rings were located.  相似文献   

Polymerase chain reaction detection of single nucleotide polymorphisms in the chicken mitochondrial D-loop region     

Takashi HARUMI  Akiko SANO  Hiroshi KAGAMI  Takahiro TAGAMI  Yuko MATSUBARA  Mitsuru NAITO 《Animal Science Journal》2004,75(6):503-507

The D‐loop region in mitochondrial DNA (mtDNA) was sequenced and compared among six chickens. Eleven single nucleotide polymorphisms (SNP) were observed. For six of the SNP sites, polymerase chain reaction (PCR) primers that had each base (A, C, G and T) as the penultimate base at the 3′ end (N2 base) and bases specific to both alleles at the 3′ end were produced to type the polymorphisms. Twenty‐one out of 96 primers succeeded in distinguishing the SNP by the presence or absence of PCR product. This method provides an easy way to discriminate SNP in chicken mtDNA.  相似文献   

The luteotrophic function of galectin-1 by binding to the glycans on vascular endothelial growth factor receptor-2 in bovine luteal cells     

Masahiro SANO  Kazuhisa HASHIBA  Junko NIO-KOBAYASHI  Kiyoshi OKUDA 《The Journal of reproduction and development》2015,61(5):439-448

The corpus luteum (CL) is a temporary endocrine gland producing a large amount of progesterone, which is essential for the establishment and maintenance of pregnancy. Galectin-1 is a β-galactose-binding protein that can modify functions of membrane glycoproteins and is expressed in the CL of mice and women. However, the physiological role of galectin-1 in the CL is unclear. In the present study, we investigated the expression and localization of galectin-1 in the bovine CL and the effect of galectin-1 on cultured luteal steroidogenic cells (LSCs) with special reference to its binding to the glycans on vascular endothelial growth factor receptor-2 (VEGFR-2). Galectin-1 protein was highly expressed at the mid and late luteal stages in the membrane fraction of bovine CL tissue and was localized to the surface of LSCs in a carbohydrate-dependent manner. Galectin-1 increased the viability in cultured LSCs. However, the viability of LSCs was decreased by addition of β-lactose, a competitive carbohydrate inhibitor of galectin-1 binding activity. VEGFR-2 protein, like galectin-1, is also highly expressed in the mid CL, and it was modified by multi-antennary glycans, which can be recognized by galectin-1. An overlay assay using biotinylated galectin-1 revealed that galectin-1 directly binds to asparagine-linked glycans (N-glycans) on VEGFR-2. Enhancement of LSC viability by galectin-1 was suppressed by a selective inhibitor of VEGFR-2. The overall findings suggest that galectin-1 plays a role as a survival factor in the bovine CL, possibly by binding to N-glycans on VEGFR-2.  相似文献   

A net design for estimating the vertical distribution of larval and juvenile fishes on a tidal mudflat   总被引：2，自引：1，他引：1  

Kouki  KANOU  Mitsuhiko  SANO  Hiroshi  KOHNO 《Fisheries Science》2004,70(4):713-715

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Comparison of the bioactive components of the ecotypes and cultivars of plantain (Plantago lanceolata L.) herbs     

Mohammad AL-MAMUN  Daisuke ABE  Hisayoshi KOFUJITA  Yoshifumi TAMURA  Hiroaki SANO 《Animal Science Journal》2008,79(1):83-88

The concentrations of the bioactive components, acteoside, aucubin and catalpol, and the production yield, crude protein (CP) and crude ash contents in 25 ecotypes (Et) of plantain (PL, Plantago lanceolata L.), found in northern Japan, and commercial cultivars, Grasslands Lancelot (GL) and Ceres Tonic (CT), were investigated. The quantitative determination of bioactive components was performed by high performance liquid chromatography. The acteoside content of some of the 25 Et was significantly higher (P < 0.05) than CT and numerically higher than GL and other Et. Most of the Et had numerically higher concentrations of catalpol and aucubin than the GL and CT. The CP and crude ash content of the Et were comparable with those of the GL and CT. The dry matter (DM) yields of some of the Et was numerically greater than that of the cultivars. The present data suggest that most of the Et are comparable to the cultivars in respect to the concentration of bioactive components and DM yield as well as CP and crude ash contents and could be used as pasture like GL and CT.  相似文献   

Short-term effects of a mass coral bleaching event on a reef fish assemblage at Iriomote Island, Japan   总被引：3，自引：0，他引：3  

Mitsuhiko  SANO 《Fisheries Science》2004,70(1):41-46

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Systemic Mycobacteriosis in an Aborted Thoroughbred Fetus in Japan     

Yuto SANO  Kazuya MATSUDA  Keisuke OSAKI  Taku MIYASHO  Tomonori TSUDA  Hiroyuki TANIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1617-1621

A male Thoroughbred fetus was aborted on day 251 of pregnancy. Gross and histological examinations detected systemic granulomatous lesions in many superficial and visceral lymph nodes and organs including the liver, tonsils, lungs, thymus, spleen, right thyroid gland and gastrointestinal tract, and suppurative placentitis, pyogranulomatous amnionitis and intralesional acid-fast bacilli were also detected. An examination of the DNA base sequence of the β subunit of RNA polymerase demonstrated that Mycobacterium avium strain 104 had infected several organs. To the best of our knowledge, this is the first report of equine fetal mycobacterial infection in Japan.  相似文献