Adaptation and limitations of established hemagglutination inhibition assays for the detection of porcine anti-swine influenza virus H1N2 antibodies.   总被引：1，自引：0，他引：1  

Bradley C Long  Tony L Goldberg  Sabrina L Swenson  Gene Erickson  Gail Scherba 《Journal of veterinary diagnostic investigation》2004,16(4):264-270

Hemagglutination inhibition (HI) has been a reliable method for determining porcine antibody levels to the well-characterized swine influenza virus (SIV) H1N1 and H3N2 subtypes. However, the recent emergence of the novel H1N2 serotype of SIV and the persistence of 2 other serotypes (H1N1 and H3N2) in the United States swine population represents a significant challenge to diagnostics. Both standardized and modified HI protocols were used in a blinded study to examine a collection of 50 control sera representing a total of 12 swine that were experimentally inoculated with one of the 3 SIV subtypes. Using these control sera data, a statistical basis for analysis was established in an attempt to classify 30 field sera with known case histories or seroprevalance into SIV serotype categories. By this approach 57% of the field sera could be classified into specific categories. The remaining samples that could not be classified reliably were most likely composed of heterogeneous anti-SIV antibody populations. These results indicate that although serological differentiation might be possible in a controlled environment, applications of these methods to field samples are currently problematic. Approaches other than HI will be required to fulfill the current need for SIV diagnostics and surveillance when specific serotype identification is required.  相似文献   

Perception of challenges in management of neurological cases in the emergency room     

Franziska B. Meyer Dr med vet  Sabrina N. Hoehne Dr med vet  DACVECC  DECVECC  Vishal D. Murthy DVM  DACVIM  Arianna Maiolini Dr med vet  DECVN  PhD  Veronika M. Stein Dr med vet  DECVN  PhD  Justus M. K. Rathmann  Julien Guevar DVM  MVM  DECVN 《Journal of Veterinary Emergency and Critical Care》2023,33(1):38-46

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Detection of Helicobacter spp. DNA in the oral cavity of dogs     

Recordati C  Gualdi V  Tosi S  Facchini RV  Pengo G  Luini M  Simpson KW  Scanziani E 《Veterinary microbiology》2007,119(2-4):346-351

The mode of acquisition of gastric Helicobacter spp. infection in dogs has not been determined. It is suspected that oral-oral and faecal-oral transmission may be involved. The present study sought to determine if Helicobacter spp. DNA is present in the oral cavity of healthy and vomiting dogs. Thirty-eight pet dogs (27 vomiting and 11 clinically healthy) were studied. The presence of Helicobacter spp. was determined by single and nested PCR evaluation of DNA extracted from saliva, dental plaque and gastric biopsy samples. Helicobacter spp. DNA was detected by nested PCR in 36 (94.7%) gastric biopsies, 17 (44.7%) dental plaque and 19 (50%) saliva samples out of the 38 dogs examined. Overall 27 (71.1%) dogs screened by nested PCR were found to harbour Helicobacter spp. DNA in the oral cavity (dental plaque and/or saliva). There was no significant difference in the prevalence of Helicobacter spp. DNA in the oral cavity of vomiting and healthy dogs, and the time from vomiting to oral sampling did not have significant impact. This study confirms the high prevalence of gastric Helicobacter spp. infection in dogs, and reveals that Helicobacter spp. DNA is detectable in the oral cavity of over 70% of dogs. These findings support the possibility of oral-oral transmission between dogs and that the canine oral cavity may act as source of non-pylori Helicobacter spp. infection for humans.  相似文献   

The Influence of Landscape Structure on Female Roe Deer Home-range Size   总被引：5，自引：0，他引：5  

Sonia?Sa?dEmail author  Sabrina?Servanty 《Landscape Ecology》2005,20(8):1003-1012

Animal distribution and abundance are greatly affected by the availability of their food resources, which also depends on landscape structure. Lothar hurricane in 1999 had profoundly modified the structure of the forests in France, affecting the habitat quality of ungulates. We tested whether the variations in home-range size of 23 female roe deer were influenced by the fragmentation of the landscape caused by Lothar in the Chizé forest, namely by the increase in heterogeneity associated with the localized massive tree felling. Home-range size was studied in the summers of 2001 and 2002 and we found that variation in home-range size was mainly explained by only one landscape variable: edge density. Home-range size decreased as edge density increased, which is consistent with the fact that edges are good browsing habitats for roe deer. The result of this study suggests that, after 2 years, the hurricane had improved the quality of the home ranges by creating more forest heterogeneity and increasing the contacts between the different vegetation patches within the home range. These results highlight the fact that spatial heterogeneity is likely to be a key factor influencing the distribution and local population density.  相似文献   

What is your diagnosis? Dystrophic mineralization     

Brounts SH  Baird AN  Baird DK 《Journal of the American Veterinary Medical Association》2005,226(3):349-350

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Nanotechnology-based approach for safer enrichment of semen with best spermatozoa     

Casey L.Durfey  Sabrina E.Swistek  Shengfa F.Liao  Mark A.Crenshaw  Henry J.Clemente  Rooban V.K.G.Thirumalai  Christy S.Steadman  Peter L.Ryan  Scott T.Willard  Jean M.Feugang 《畜牧与生物技术杂志(英文版)》2019,(2)

Background: Advances in nanotechnology have permitted molecular-based targeting of cells through safe and biocompatible magnetic nanoparticles(MNP). Their use to detect and remove damaged spermatozoa from semen doses could be of great interest. Here, MNP were synthesized and tested for their ability to target apoptotic(annexin V) and acrosome-reacted(lectin) boar spermatozoa, for high-throughout retrieval in a magnetic field(nanoselection). The potential impacts of nanoselection on sperm functions and performance of offspring sired by sperm subjected to nanoselection were determined. Fresh harvested and extended boar semen was mixed with various amounts(0, 87.5, and 175 μg) of MNP-conjugates(Annexin V-MNP or Lectin-MNP) and incubated(10 to15 min) for 37 °C in Exp. 1. In Exp. 2, extended semen was mixed with optimal concentrations of MNP-conjugates and incubated(0, 30, 90, or 120 min). In Exp. 3, the synergistic effects of both MNP-conjugates(87.5 μg– 30 min)on spermatozoa was evaluated, followed by sperm fertility assessments through pregnancy of inseminated gilts and performance of neonatal offspring. Sperm motion, viability, and morphology characteristics were evaluated in all experiments.Results: Transmission electron microscopy, atomic force microscopy, and hyperspectral imaging techniques were used to confirm attachment of MNP-conjugates to damaged spermatozoa. The motility of nanoselected spermatozoa was improved(P 0.05). The viability of boar sperm, as assessed by the abundance of reactive oxygen species and the integrity of the acrosome, plasma membrane, and mitochondrial membrane was not different between nanoselected and control spermatozoa. The fertility of gilts inseminated with control or nanoselected spermatozoa, as well as growth and health of their offspring were not different between(P 0.05).Conclusions: The findings revealed the benefit of magnetic nanoselection for high-throughput targeting of damaged sperm, for removal and rapid and effortless enrichment of semen doses with highly motile, viable,and fertile spermatozoa. Therefore, magnetic nanoselection for removal of abnormal spermatozoa from semen is a promising tool for improving fertility of males, particularly during periods, such as heat stress during the summer months.  相似文献   

Oogenesis is accompanied by cyclic morphological changes in hepatocytes of Neotropical freshwater fish Piabina argentea          下载免费PDF全文

Ralph G. Thomé  Isabella Cristina de Oliveira Cardoso  Sabrina Elisa de Oliveira  Hélio Batista dos Santos 《Anatomia, histologia, embryologia》2018,47(5):466-474

We determined for the first time the reproductive biology of Piabina argentea through macroscopic and microscopic analysis of ovaries and evaluated the morphological changes in hepatocytes. Two hundred and 46 specimens were collected, 204 females and 42 males, between March 2014 and February 2015. Biometrics data were obtained. From females, gonad and liver samples were conducted to histological, histochemical and immunohistochemical techniques. Mature ovaries were used to determine absolute and relative fecundity. Total length and body weight values indicated that females were larger than males. The estimated weight–length ratio showed negative allometric growth. The absolute fecundity average was 171.83 ± 59.89 oocytes per ovary. In addition, females spawning capable and regressing stages were found throughout the sampling period and the presence of all oocyte types in regressing stage ovaries indicated asynchronous oocyte development and multiple spawning. From regenerating to spawning capable stage the oocytes accumulated yolk in cytoplasm became bigger. While in the liver hepatocytes with a larger cell area during regenerating stage and proliferative activity in the spawning capable stage were observed. Thus, our results indicate that P. argentea had an opportunistic reproductive strategy and cyclic morphological changes of hepatocytes occurred during the oogenesis.  相似文献   

Severe hypernatremia and transient azotemia in a cat following inadvertent intravenous administration of a commercial polyethylene glycol solution     

Sabrina N. Hoehne  Casey J. Kohen  Birgit Puschner  Ingrid Gennity  Simon P. Hagley  Kate S. Farrell  Karin Unger  Laura A. Cagle  Karl E. Jandrey 《Journal of Veterinary Emergency and Critical Care》2019,29(6):690-695

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Ultrasonographic differentiation between portal venous and parenchymal gas may be important for the prognosis of canine and feline hepatic emphysema: 37 cases     

Sabrina Manfredi  Martina Fabbi  Mattia Bonazzi  Fabio Leonardi  Francesca Miduri  Ilaria Parroccini  Eleonora Daga  Giacomo Gnudi  Antonella Volta 《Veterinary radiology & ultrasound》2019,60(6):734-744

The aim of this retrospective, cross‐sectional, study was to evaluate clinical findings and outcomes for different ultrasonographic patterns of hepatic emphysema in dogs and cats. Dogs and cats with an ultrasonographic diagnosis of hepatic emphysema and a known outcome, from January 2010 to January 2018, were enrolled. The following data were recorded from medical and ultrasonographic records: ultrasonographic patterns of hepatic emphysema (parenchymal, portal venous, biliary), clinical signs, laboratory findings, and outcomes (favorable, poor). A total of 33 dogs and four cats met the inclusion criteria. Among these, 23 cases were classified as hepatic portal venous gas, 10 as parenchymal emphysema, and four as biliary emphysema. Clinical diagnosis categories were as follows: infection/sepsis (9), gastro‐intestinal disease (9), iatrogenic (9), trauma (5), and liver neoplasia (5). An increase in serum liver enzymes was significantly associated with parenchymal emphysema (P = .03). Other clinical and laboratory findings were not associated with the type of hepatic emphysema. Hepatic portal venous gas was mostly transient in patients with ultrasonographic follow‐up. The overall mortality was 40.5%. A significant difference was found between mortality by portal venous gas (21.7%) and mortality by parenchymal emphysema (90%) (P = .003). In conclusion, the ultrasonographic differentiation of hepatic emphysema between hepatic portal venous gas and parenchymal emphysema may be important for the prognosis of hepatic emphysema. The presence of parenchymal emphysema may be a poor prognostic indicator, while hepatic portal venous gas may be more benign. However, ultrasound findings should be carefully evaluated in the context of clinical findings.  相似文献   

Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time reverse-transcription polymerase chain reaction and virus isolation     

Christa K. Goodell  Jianqiang Zhang  Erin Strait  Karen Harmon  Devi Patnayak  Tracy Otterson  Marie Culhane  Jane Christopher-Hennings  Travis Clement  Pamela Leslie-Steen  Richard Hesse  Joe Anderson  Kevin Skarbek  Amy Vincent  Pravina Kitikoon  Sabrina Swenson  Melinda Jenkins-Moore  Jodi McGill  Rolf Rauh  William Nelson  Catherine O’Connell  Rohan Shah  Chong Wang  Rodger Main  Jeffrey J. Zimmerman 《Canadian journal of veterinary research》2016,80(1):12-20

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