Identification of toxigenic Pasteurella multocida in atrophic rhinitis of pigs by in vitro characterisation     

GJ EAMENS  PD KIRKLAND  MJ TURNER  JA GARDNER†  MP WHITE‡  CL HORNITZKY 《Australian veterinary journal》1988,65(4):120-123

Toxigenic strains of Pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. Cells which were young and densely confluent were best suited to this assay. The incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. The agarose overlay method correctly identified 11 of 11 reference strains of Pasteurella multocida, and visible cytotoxic changes were present in the monolayers after 48 to 65 h. Outbreaks of the enzootic form of atrophic rhinitis in 2 New South Wales piggeries were associated with the isolation of toxigenic type D strains of P. multocida.  相似文献   

Molecular biologic methods for epidemiological precision characterization of disease agents using Salmonella enteritidis from poultry as an example     

R Helmuth  M A Montenegro  A Steinbeck  A Seiler  O Pietzsch 《Berliner und Münchener tier?rztliche Wochenschrift》1990,103(12):416-421

The principles of molecular biological methods for epidemiological investigation of infectious bacteria are described. They were applied for differentiating S. enteritidis isolates originating from poultry. Among the methods described are the determination of the chromosomal DNA fingerprints (BRENDA), the methods of multilocus enzyme electrophoresis, plasmid profiles, and the outer membrane protein as well as lipopolysaccharide pattern. It turned out, that S. enteritidis strains isolated before 1987, in 1988 and the ones originating from Great Britain did not differ in anyone of these criteria. Consequently one has to assume, that the strains recently causing problems originate from the population of S. enteritidis which had existed in poultry before.  相似文献   

LOW-FIELD MAGNETIC RESONANCE IMAGING OF A PYOCEPHALUS AND A SUSPECTED BRAIN ABSCESS IN A GERMAN SHEPHERD DOG     

Gabriela  Seiler  Dr. Med. Vet.  Sigitas  Cizinauskas  Dr. Med. Vet.  Jürg  Scheidegger  Dr. Med. FMH  Johann  Lang  Dr. Med. Vet. 《Veterinary radiology & ultrasound》2001,42(5):417-422

Magnetic resonance imaging was performed on an eight-year-old, neutered female German Shepherd dog with a history of acute depression, inappetence, and hyperthermia. A lesion in the cerebrum was suspected. Possible differential diagnoses were meningoencephalitis, neoplasia, and vascular lesion (infarction, bleeding). A ring enhancing lesion was found in the basal ganglia on the left side with edema of the surrounding brain tissue. A similar mass lesion was present in the right pterygoid musculature. With inversion recovery sequences an altered composition of the cerebrospinal fluid (CSF) in the left lateral ventricle could be detected. CSF analysis confirmed a pyocephalus, probably due to rupture of a brain abscess into the left lateral ventricle.  相似文献   

Cardiomyopathy and woolly haircoat syndrome of Hereford cattle     

GJ STORIE  JA GIBSON  JD TAYLOR 《Australian veterinary journal》1991,68(3):119-119

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Histiocytic Sarcoma with Central Nervous System Involvement in Dogs: 19 Cases (2006–2012)          下载免费PDF全文

C.L. Mariani  M.K. Jennings  N.J. Olby  L.B. Borst  J.C. Brown Jr  I.D. Robertson  G.S. Seiler  E. MacKillop 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(2):607-613

Background

Reports of histiocytic sarcoma (HS) involving the central nervous system (CNS) are sparse and consist mainly of case reports describing 1–3 animals.

Objective

The objective of this study was to report the signalments, clinical signs, clinicopathologic and diagnostic imaging findings, treatment, and outcome of a series of dogs with HS and CNS involvement.

Animals

Nineteen dogs with HS examined at veterinary referral hospitals.

Methods

Retrospective case series. Medical records were reviewed and cases with a histopathological diagnosis of CNS HS were included in the study. Diagnostic imaging studies of the CNS were evaluated and histopathologic samples were reviewed to confirm the diagnosis.

Results

Retrievers and Pembroke Welsh Corgis were overrepresented in this cohort of dogs. Tumors involved the brain in 14 dogs and the spinal cord in 5. In 4 dogs, HS was part of a disseminated, multiorgan process whereas it appeared confined to the CNS in 15 dogs. Diagnostic imaging had variable appearances although extraaxial masses predominated in the brain. There was meningeal enhancement in all dogs that was often profound and remote from the primary mass lesion. Pleocytosis was present in all dogs with CSF evaluation. Median survival was 3 days.

Conclusions and Clinical Importance

Breed predispositions appear to vary from reports of HS in other organ systems. Some unique imaging and clinicopathologic characteristics, particularly brain herniation, profound meningeal enhancement, and pleocytosis in combination with 1 or more mass lesions, might help to differentiate this neoplasm from others involving the CNS, although this requires further study.  相似文献   

ELASTOGRAPHIC EVALUATION OF NATURALLY OCCURING TENDON AND LIGAMENT INJURIES OF THE EQUINE DISTAL LIMB          下载免费PDF全文

Meghann Lustgarten  W. Rich Redding  Raphael Labens  Weston Davis  Thomas M. Daniel  Emily Griffith  Gabriela S. Seiler 《Veterinary radiology & ultrasound》2015,56(6):670-679

Compression elastography is an ultrasonographic technique that estimates tissue strain and may have utility in diagnosing and monitoring soft tissue injuries in the equine athlete. Recently, elastography has been proven to be a feasible and repeatable imaging modality for evaluating normal tendons and ligaments of the equine distal forelimb. The purposes of this prospective study were to investigate the ability of elastography to detect spontaneously occurring lesions of equine tendons and ligaments diagnosed with magnetic resonance imaging (MRI) and gray‐scale ultrasound (US) and to characterize the differences in the elastographic appearance of acute vs. chronic injuries. Fifty seven horses with a total of 65 lesions were evaluated. Images were assessed quantitatively and qualitatively. Acute lesions were found to be significantly softer (P < 0.0001) than chronic lesions (P < 0.0001) and the stiffness of lesions increased with progression of healing (P = 0.0138). A negative correlation between lesion hypoechogenicity and softness was appreciated with more hypoechoic lesions appearing softer (P = 0.0087) and more hyperechoic regions harder (P = 0.0002). A similar finding occurred with increased signal intensity on short tau inversion recovery (STIR) and proton density (PD) MRI sequences correlating with increased softness on elastography (P = 0.0164). Using US and MRI as references, commonly encountered soft tissue injuries of the equine distal limb could be detected with elastography. However, elastography was limited for detecting small, proximal injuries of the hindlimb proximal suspensory ligament. Elastographic evaluation of equine tendons and ligaments may allow better characterization of lesion chronicity and severity, and sequential examinations may optimize lesion management, rehabilitation, and return to training.  相似文献   

Antibodies to influenza A virus (H1 and H3) in companion animals in Iowa, USA     

Seiler BM  Yoon KJ  Andreasen CB  Block SM  Marsden S  Blitvich BJ 《The Veterinary record》2010,167(18):705-707

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The structure of the upper atmosphere of mars: In situ accelerometer measurements from mars global surveyor   总被引：1，自引：0，他引：1  

GM Keating  SW Bougher  RW Zurek  RH Tolson  GJ Cancro  SN Noll  JS Parker  TJ Schellenberg  RW Shane  BL Wilkerson  JR Murphy  JL Hollingsworth  RM Haberle  M Joshi  JC Pearl  BJ Conrath  MD Smith  RT Clancy  RC Blanchard  RG Wilmoth  DF Rault  TZ Martin  DT Lyons  PB Esposito  MD Johnston  al et 《Science (New York, N.Y.)》1998,279(5357):1672-1676

The Mars Global Surveyor (MGS) z-axis accelerometer has obtained over 200 vertical structures of thermospheric density, temperature, and pressure, ranging from 110 to 170 kilometers, compared to only three previous such vertical structures. In November 1997, a regional dust storm in the Southern Hemisphere triggered an unexpectedly large thermospheric response at mid-northern latitudes, increasing the altitude of thermospheric pressure surfaces there by as much as 8 kilometers and indicating a strong global thermospheric response to a regional dust storm. Throughout the MGS mission, thermospheric density bulges have been detected on opposite sides of the planet near 90 degreesE and 90 degreesW, in the vicinity of maximum terrain heights. This wave 2 pattern may be caused by topographically-forced planetary waves propagating up from the lower atmosphere.  相似文献   

Staphylococcal protein A labeled with horseradish peroxidase for the immunohistologic diagnosis of canine autoimmune diseases   总被引：1，自引：0，他引：1  

R J Seiler 《American journal of veterinary research》1983,44(2):195-200

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Ultrashort hypnoanalgesia with alfentanyl and etomidate in the dog--circulatory and respiratory studies     

W Erhardt  R Seiler  V Riedl  G Aschenbrenner  G Blümel 《Berliner und Münchener tier?rztliche Wochenschrift》1985,98(12):413-417

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