异噻菌胺原药高效液相色谱分析方法研究     

董文凯  姜宜飞  王胜翔  郭海霞  石隆平 《农药科学与管理》2015,36(1):37-39

本文采用高效液相色谱法,以乙腈+磷酸溶液为流动相,使用ZORBAX SB-C18色谱柱和二极管阵列检测器,在280nm波长下对异噻菌胺原药进行分离和定量分析。结果表明,该分析方法的线性相关系数为1.000 0,标准偏差为0.39,变异系数为0.39%,平均回收率为100.08%。  相似文献   

Infection of human enterovirus 71 in immunosuppressed rhesus monkeys     

WANG Jing-jing  ZHANG Xue-mei  ZHAO Hong-ling  DONG Cheng-hong  PU Jing  LIAO Yun  WANG Li-chun  NA Rui-xiong  LIU Long-ding  ZHANG Ying  YANG Li-xian  WANG Qing-ling  SHEN Dong  WANG Xi  LI Qi-han 《园艺学报》2011,27(7):1249-1256

AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future.  相似文献   

Nutrient cycling and biomass flows in a low-quality forest stand improvement system in the Lesser Khingan Range of China     

Jinzhuo Wu  Linlin Kong  Xibin Dong 《Journal of Sustainable Forestry》2018,37(6):555-573

The material flow and bulk internal flow analyses were used to establish a material accumulation and cycling model for a low-quality forest stand improvement system and a series of processes were considered. The model was applied in a one-hectare low-quality forest plot in the Lesser Khingan Range of China. Results showed that during 1997–2007, the stands absorbed 270.19 kg of N, 74.28 kg of P, and 124.39 kg of K from soils, 51.82 kg of N and 2.38 kg of P were directly absorbed by foliage, and 16.25 kg of K was released to soils by eluviation. Until 2007, the accumulated nutrients in the stands included 236.91 kg of N, 65.28 kg of P, and 108.55 kg of K. When horizontal strip clearcutting was applied in 2007, 50% accumulated nutrients in the stands were shifted due to harvesting operations, and 212.74 kg of N, 26.97 kg of P, and 98.88 kg of K were accumulated in soils, declining by 9.47% for N, 3.68% for P, and 17.60% for K, respectively, compared with year 1997. 94.61 t per hectare of biomass was generated, of which the biomass in stands accounted for 87.36%. The felled tree biomass was 36.89 t per hectare, of which 84.90% and 10.03% of biomass were utilized in terms of logs and other means, and the rest was left on site.  相似文献   

菜籽油实罐储存品质变化规律研究     

周天智  王东  吴秋蓉  许建华  邱艳  陈冉  李婕妤  刘爱兰  孙朝军  陈军 《粮食储藏》2017,(2):40-47

通过对2010~2014年大、中、小不同罐容实罐储存的四级菜籽油,采取定点、定罐、定层、定期跟踪观察油罐"三温"变化并取样检测,发现罐温、油温均随着气温的变化而变化,其中罐温最高,油温从上至下依次降低。菜籽油的水分及挥发物、色泽、气味、滋味、加热试验(280℃)等指标在储藏期间变化均较平稳,酸值和过氧化值均呈不断上升趋势,且不同年限的菜籽油其过氧化值上升幅度明显高于酸值。油罐容量越大,满罐存储时其存油品质的稳定性越好。罐内上、中、下层酸值变化差异不明显,上层和底层的过氧化值明显高于中层。严格控制好入罐油品质量,菜籽油储藏4~5年后酸值、过氧化值等质量指标仍可保持在国家限量标准以内。  相似文献   

遵义市辣椒产业现状及发展战略     

彭诗云  谢挺  罗燚  毛东  胡建宗  易伦 《辣椒杂志》2017,(3):33-36

遵义市是中国辣椒之都,位于全国最大的辣椒主产区西南和华中的中心区域,也是全国辣椒消费重辣区,其交通便捷,环境优美,辣椒产业文化丰富。当前遵义辣椒产业正处在基地优化、技术升级和产业提升的关键时期,亟需理清发展思路。通过对遵义辣椒产业现状的阐述与问题的分析,从而针对性地提出了发挥种植优势、突出产品特色,优化产区布局、推广生态化栽培,培育大型加工企业、促进加工产业升级与转型等战略,以促进遵义辣椒产业的发展。  相似文献   

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro     

CHEN Tian-tian  CHENG Fu-liang  ZHANG Jian-mei  NIE Zhao-jing  FANG Dong  FAN Mei-na  LI Fu-hui  CHENG He-gang 《中国畜牧兽医》2015,42(9):2474-2480

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.  相似文献   

苹果‘长富2号’开花调控转录因子基因MdIDD7的克隆及表达分析     

齐思言  邢利博  张东  杜利莎  李有梅  樊胜  马娟娟  赵彩平  韩明玉 《园艺学报》2017,44(5):828-838

以‘长富2号’苹果为试验材料,采用RT-PCR的方法,从其芽中克隆得到INDETERMINATE DOMAIN(IDD)转录因子基因MdIDD7,其开放阅读框长度为1 626 bp,编码541个氨基酸。序列比对和结构域分析表明,该转录因子含有1个核定位信号和4个高度保守的锌指蛋白结构域。系统进化树分析表明,MdIDD7与白梨(Pyrus×bretschneideri,XP_009364602.1)、桃(Prunus persica,XP_007225628.1)和梅(Prunus mume,XP_008220893.1)聚在一起。实时荧光定量PCR表明,MdIDD7在‘长富2号’不同组织(茎、叶、花、果和芽)中均有表达,其中芽的表达量最高。花后40~60 d,MdIDD7在易成花品种‘烟富6号’芽中表达量显著高于难成花品种‘长富2号’。"小年"树顶芽组织中MdIDD7的表达量显著高于"大年"树。在花芽诱导前期,外源GA处理诱导MdIDD7下调表达,而蔗糖处理诱导其上调表达,说明MdIDD7响应激素和糖信号,促进苹果成花。  相似文献   

钾肥减量分期施用对莴笋产量和品质的效应研究     

范晓伟  谷守宽  陈益  袁婷  杨东  王正银 《中国农学通报》2017,33(11):25-34

通过大田试验研究了紫色菜园土壤钾肥减量分期施用对莴笋产量和品质的影响。结果表明：两个试验点钾肥减量10%~30%分期施用对莴笋产量有提高作用，各处理以K2（钾肥减量10%分期等量追施）效应最好，增产最大（4.2%和4.5%）、施肥利润最高（84923元/hm²和72075元/hm²）。两个试验点莴笋叶维生素C含量均呈下降趋势，K2处理较常规施肥(CK)处理均显著降低莴笋叶维生素C含量（8.0%和8.7%）；而莴笋茎维生素C含量变化规律不一致。钾肥减量分期施用降低两个试验点莴笋叶和茎硝酸盐含量，以K4处理（钾肥减量30%分期等量追施）降低作用最大；从莴笋叶和茎糖组分的均衡性看，以K3处理最好。钾肥减量分期施用对两个试验点莴笋叶必需氨基酸含量有显著增加作用，改善叶氨基酸品质性状，但降低莴笋茎必需氨基酸含量和品质性状；莴笋氨基酸品质性状以K4处理为最优，其次是K2处理。综合考虑莴笋产量和品质，紫色菜园土壤以K2处理（钾肥减量10%分期等量追施）最优。  相似文献   

苹果赤霉素信号转导因子MdGAMYB的克隆和表达分析   总被引：1，自引：0，他引：1  

樊胜  雷超  辛明志  邵红霞  Muhammad Mobeen Tahir  张东  韩明玉 《园艺学报》2017,44(5):817-827

以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子MdGAMYB,对其进行生物信息学和表达分析。结果表明,MdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明MdGAMYB编码的蛋白存在多个糖基化位点和磷酸化位点;序列分析表明,Md GAYMB和其他物种的GAMYB蛋白有很高的相似性,均含有保守的R2R3 DNA结合域和GAMYB家族所特有的Box1,Box2和Box3保守区域;系统进化分析表明,Md GAYMB与梨、梅花、草莓、枣和葡萄等的GAMYB蛋白具有较高的同源性。实时荧光定量PCR分析表明,Md GAYMB具有组织表达特异性,在叶片、花和芽中的表达量较高。外源GA3处理抑制了花芽孕育和翌年成花,抑制MdGAMYB的表达。在易成花品种‘烟富6号’中的表达量高于难成花品种‘长富2号’。  相似文献   

卧式香蕉秸秆粉碎还田机甩刀的设计与优化     

唐宁宁  李粤  梁栋  张喜瑞  周腾  宋雅婷  吴思浩  王自强 《农机化研究》2017,(4)

甩刀是卧式香蕉秸秆粉碎还田机的重要工作部件,合理的结构和参数设计可改善整机的工作性能,减少机具振动,提高香蕉秸秆粉碎质量。通过应用Adams、Solidworks Simulation软件对刀辊进行模态仿真,对刀片的运动及受力进行理论分析,以确定刀片的最优的基本参数和排列方式。结果表明:刀片厚度为8mm、弯折角为1 3 0°时,刀片的变形量小,粉碎效果好;V字形排列方式能有效避免刀辊的共振,延长使用寿命。  相似文献