Effect of monocyte-secreted VEGF induced by electrical burn serum on monocyte-endothelial cell adhesion     

RUAN Qiong-fang  ZHAO Chao-li  YE Zi-qing  XIE Qiong-hui  XIE Wei-guo 《园艺学报》2015,31(4):755-758

AIM: To observe the level of vascular endothelial growth factor (VEGF) secreted by monocytes cultured with electrical burn serum, and to explore the effect of VEGF on monocyte-endothelial cell adhesion.METHODS: The electrical burn serum of the rat was prepared. The normal serum from the rats without treating electric current was also collected for control. The contents of VEGF and its soluble receptor sFlt-1 in electrical burn group were determined by double-antibody sandwich ELISA. THP-1 cells were randomly divided into normal serum group and electrical burn serum group. The contents of VEGF and sFlt-1 in the culture supernatants were measured by double-antibody sandwich ELISA. THP-1 cells were also randomly divided into another 4 groups:normal serum group, electrical burn serum group, normal serum+inhibitor group and electrical burn serum+inhibitor group. THP-1 cells, which were incubated with the serum for 3 h and 6 h, were labeled with calcein-AM and then were added into the well with monolayer of endothelial cell line EA.hy926 to detect monocyte-endothelial cell adhesion.RESULTS: The levels of serum VEGF of the rats with electrical burns were significantly increased, the levels of serum sFlt-1 were significantly decreased as compared with the controls. The levels of VEGF secreted by THP-1 cells cultured with electrical burn serum were significantly increased, the levels of sFlt-1 were decreased correspondingly. Electrical burn serum enhanced monocyte-endothelial cell adhesion, sFlt-1 inhibited the adhesion between monocytes and endothelial cells.CONCLUSION: The monocytes exposed to the electrical burn serum secrete VEGF, which enhance the adhesion between monocytes and endothelial cells. Blockage of VEGF activity may effectively inhibit monocyte-endothelial cell adhesion.  相似文献   

纸板用大豆蛋白胶粘剂的研究   总被引：5，自引：1，他引：4  

袁超  杨光  杨波  耿玮蔚 《大豆科学》2009,28(4)

研究了尿素改性大豆分离蛋白(SPI)粘接单面涂布白板纸的最佳工艺.用尿素溶液对大豆分离蛋白进行改性,并用白板纸进行粘接,确定了其最佳工艺条件:尿素浓度3 mol·L-1、SPI含量10%、反应温度40℃、反应时间5 h,测得粘接强度为69.5125N·cm-2.单因素试验和正交试验结果表明:尿素浓度对改性SPI胶的粘接强度影响最大,其次是SPI含量和反应温度,反应时间的影响最小,尿素改性后豆胶的粘接强度有所提高.  相似文献   

湿固化胶粘接高含水率染色和固色桦木胶接性能探讨     

韦双颖  顾继友  王砥  桂成胜 《东北林业大学学报》2009,37(9)

为了系统研究不同表面状态下桦木单板的胶接性能,在比较桦木素材、染色桦木以及固色桦木表面润湿性能的基础上,采用湿固化异氰酸酯胶黏剂,对比分析了素材、染色和固色桦木3种试件在含水率30%、50%、70%和90%条件下的干状及湿状胶接强度.结果表明:随着含水率的升高,素材、染色和固色桦木表面的平衡接触角均呈上升趋势,胶接强度均呈下降趋势,并且其湿状胶接强度均明显低于各自的干状胶接强度;素材的干湿状胶合强度均明显高于染色和固色两种试件.  相似文献   

高含水率单板胶接工艺的研究   总被引：1，自引：0，他引：1  

黄元波  顾继友  李海英 《林产工业》2005,32(2):11-14

研究了单组分湿固化异氰酸酯胶粘剂胶接高含水率单板的工艺条件,详细讨论了含水率、涂胶量、加压压力、加 压时间和养生时间对胶合性能的影响。结果表明:单板含水率、涂胶量是重要的影响因子,单组分湿固化异氰酸酯胶粘 剂可胶接含水率高达100％的单板,在保证胶接强度的前提下,涂胶量仅为传统胶粘剂的1／2-1／3。  相似文献   

基于AFM的微机械表面抗粘附薄膜性能   总被引：2，自引：0，他引：2  

付永忠  丁建宁  杨继昌 《农业机械学报》2006,37(12):182-184,154

用原子力显微镜（AFM）考察施加OTS分子润滑膜前后Si（100）表面的粘附、摩擦性能，并对比了空气相对湿度、扫描速度对粘附、摩擦性能的影响效果。结果表明，施加OTS膜后可以有效降低Si（100）表面粘附力和摩擦系数，呈现较好的润滑性能，且受空气相对湿度变化的影响较小。另外，适当提高扫描探针扫描速度，可以改善Si（100）表面和OTS膜表面摩擦性能，降低摩擦力。  相似文献   

Effects of vinpocetine on inflammation of brain in rats with intracerebral hemorrhage     

SUN Jian-ping  LI Fu-ping  XU Zhao-juan  BAI Jian-ying  YANG Jiang-xia  ZHANG Xiu-fen  LI Cui-qing  ZHAO Dong 《园艺学报》2017,33(12):2139-2142

AIM: To investigate the effects of vinpocetine on inflammation of brain in intracerebral hemorrhage (ICH) rats and to explore the underlying mechanisms. METHODS: All rats were randomly divided into sham group, ICH group, ICH with low dose of vinpocetine treatment group, ICH with medium dose of vinpocetine treatment group, and ICH with high dose of vinpocetine treatment group. Except sham group, the rats in other groups were injected with type VⅡ collagenase to establish ICH model, and then the rats in vinpocetine treatment groups were received vinpocetine at 0.5, 1.0 or 1.5 mg/kg by intraperitoneal injection once a day for 7 days. After corresponding treatment, the impairment of neurological function in the rats was scored and the water content of the brain tissues was measured. Moreover, the activity of myeloperoxidase (MPO) was determined by ELISA, and the protein expression of Toll-like receptors 4 (TLR4), nuclear factor-κB(NF-κB), intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molcule-1 (VCAM-1) in the brain tissues was determined by Western blot. RESULTS: Compared with ICH group, vinpocetine treatment significantly decreased the scores of the impairment of neurological function and the water content of the brain tissues. Moreover, the activity of MPO and the protein expression of TLR4, NF-κB, ICAM-1 and VCAM-1 were also reduced after vinpocetine treatment (P<0.05). CONCLUSION: Vinpocetine improves neurological function in ICH rats via suppression of inflammation by inhibiting NF-κB signaling and expression of ICAM-1 and VCAM-1.  相似文献   

副溶血弧菌在玻璃器皿上的粘附及去除     

高璐  张琦  居翔玉  杨振泉  饶胜其  方维明 《安徽农业科学》2014,42(36):13015-13017

[目的]研究副溶血性弧菌在玻璃器皿上的粘附及去除技术.[方法]以栽玻片模拟厨房中的玻璃类和陶瓷类厨具,选用酒精、醋酸及乳酸链球菌素作为抑菌剂对人工污染粘附到载玻片上的副溶血性弧菌进行处理,通过观察副溶血性弧菌在栽玻片上的存活数和去除率考察3种抑菌剂及其协同抑菌作用.[结果]载玻片上粘附的副溶血性弧菌的活菌数经生理盐水和不同浓度的酒精、醋酸和乳酸菌链球菌素作用后均有一定程度的降低,抑菌剂组残留的活菌数（残留率）明显低于生理盐水对照组,各抑菌组的抑菌能力均随着抑菌剂浓度的增加而增强.协同抑菌试验结果表明,pH 4.0、35％酒精、1.0 g,/L乳酸链球菌素的组合能有效抑制副溶血性弧菌在玻片上的粘附,具有良好的协同效应.[结论]副溶血性弧菌在玻璃器皿上有一定的粘附力,但酒精、醋酸和乳酸菌链球菌素具有较好的除菌效果.  相似文献   

材料表面正电性对舟形藻附着的影响     

李祥筑  张敬迎  李长林  刘佳斌  王珺  刘钟馨  于晓龙 《热带作物学报》2014,35(12):2496-2501

利用单分子自组装膜(SAMs)技术,分别制备表面具有碳碳双键、甲基、氨基及混合官能团的表面膜层材料。研究表明:表面硅藻附着量是随着时间变化而呈动态变化,氨基的正电程度对舟形藻的附着有着重要影响。前H5内氨基(0.4)的附着量最小,当H12时氨基(0.4)附着量最大。材料表面的电负性和亲疏水性共同影响硅藻的附着。甲基的附着强度最大,硅藻的附着强度与附着量并无直接关系。这不仅对探讨硅藻细胞的附着行为有着意义,同时为研究应用海洋生物防污提供一种有效的途径。  相似文献   

Finger-jointing green softwood: Evaluation of the interaction between polyurethane adhesive and wood     

《Wood material science & engineering》2013,8(3-4):127-137

Abstract

Existing European standards for finger-jointing of load-bearing lumber require the wood to be dried before gluing. This article presents a study on the properties of green-glued finger joints, wet wood being bonded prior to drying. Issues to consider, in comparison to finger-jointing of dry wood, are mechanical performance of the joint, absorption of the polymer by the wood in its natural/wet state, and the chemical reactions of the adhesive on contact with water. Finger-jointed samples were tested in bending, and the glue joints analysed by optical microscopy, scanning electron microscopy and microdensitometry. A patented one-component polyurethane adhesive developed for gluing-green wood which has a moisture content usually higher than 70% was used in the study. The resulting green-glued joints showed improved strength properties in comparison to dry-jointed joints. The results confirm that green-glued joints provide a wide, continuous wood/adhesive interface from one substrate to the other. The adhesive penetrates several cells deep and the density of the wood adjacent to the joint surfaces is increased. The results also indicate that the patented adhesive forms covalent bonds to the wood substrate.  相似文献   

东海绿鳍马面鲀产卵群体结构和产卵场调查     

郑元甲  甘金宝  朱善央  姚文祖 《水产学报》1987,11(2):121-134

1.东海绿鳍马面鲀产卵群体自从开发利用以来,体长、年龄和体重组成出现了两次增大两次变小的过程。即1974—1976年和1981—1983年的个体较大,1977—1980年和1984年的个体较小,而1985年的个体最小。但性腺成熟系数却在提高,表明资源的密度在降低。 2.绿鳍马面鲀不同生活阶段的性比不一样,产卵期雄鱼的比例最高,越冬期居次,索饵期雌雄比例基本相等。 3.从产卵个体、仔稚鱼和产卵盛期渔获量的分布判断东海绿鳍马面鲀的产卵场在25°30′—30°00′N、122°00′—126°30′E海区,范围较广,主要产卵场在25°45′—27°00′N、122°00′—123°30′E,水深100—120米的海区。 4.东海绿鳍马面鲀的产卵期在3月底至5月下旬,4月中下旬为产卵盛期。 5.从雌鱼性腺V_A、V_B期比例的周日变化判断绿鳍马面鲀产卵的主要时间在23时至翌日11时。 6.从绿鳍马面鲀人工授精卵附着实验的结果和产卵场环境条件的对照,说明海洋里的贝砾类、海藻、海绵体和珊瑚等物体是马面鲀卵的良好附着基。  相似文献