番茄绿果与橙果间果实颜色及主要色素含量的遗传研究   总被引：1，自引：0，他引：1  

吴浪  赵菁菁  罗佳伟  王晨  李方曼  李云洲  张颜  梁燕 《中国蔬菜》2017,1(3):29-37

对番茄组合绿樱(绿果)×金珠1号(橙果)的6个世代遗传群体(P1、P2、F1、BC1、BC2和F2)进行果色性状、番茄红素含量、叶绿素含量和胡萝卜素含量等的遗传规律分析。结果表明:正反交F1的果色性状无明显差异,而色素含量存在显著差异;说明番茄果色性状受核基因控制,而色素含量遗传除受核基因控制外还可能存在胞质效应。采用多世代联合分析法的分析结果表明,番茄绿果与橙果间的果色性状符合2对加性主基因+加性-显性多基因(MX2-A-AD)遗传模型,其BC1、BC2和F2主基因遗传率分别为73.42%、78.25%和61.41%,多基因遗传率分别为22.87%、15.35%和34.94%,即果色性状遗传的主基因遗传力较强;叶绿素含量符合1对负向显性主基因+加性-显性多基因(MX1-AEND-AD)遗传模型,其BC1、BC2和F2主基因遗传率分别为0、1.73%和0.65%,多基因遗传率分别为45.47%、0和37.82%,即主基因遗传力在BC2群体中最高,多基因遗传力在BC1群体中最高;番茄红素含量与胡萝卜素含量均符合2对加性-显性-上位性主基因+加性-显性多基因(MX2-ADI-AD)遗传模型,其BC1、BC2和F2主基因遗传率分别为75.74%、1.79%、84.26%和61.53%、87.21%、81.05%,多基因遗传率分别为20.32%、74.12%、12.68%和0.68%、0、0,表明番茄红素含量和胡萝卜素含量的主基因遗传力较强。  相似文献   

Do forest and health professionals presume that forests offer health benefits,and is cross-sectional cooperation conceivable?     

《Urban Forestry & Urban Greening》2017

In high-income countries depression and cardiovascular diseases were predicted to be the two leading causes of DALYs in the year 2030. Private-life stress fosters both kinds of diseases. Scientific findings already show that forest exposure has stress-reducing effects. Particularly in Japan, people have practiced forest bathing to improve their health. The German population also has a strong connection to its forests, and forest law allows forest access, regardless of ownership structure. Hence, the question arises of whether forest exposure could be used in Germany as a kind of stress-coping strategy. To a certain degree, the success of such a strategy in Germany would require the participation of the stakeholders that are active in both the health and the forestry sectors. Therefore, it seems necessary to gain insight into German forest and health professionals' understanding and attitude concerning forest-related health benefits. For this reason, in this pilot study, guideline interviews with professionals of both sectors and with professionals standing in between these professions were conducted, recorded and transcribed. On the one hand, each professional’s presumptions regarding the health-fostering effects of forests were investigated, derived from their subjective certainty that forest exposure has health-fostering effects. In addition, a thought experiment was used to estimate the level of willingness to cooperate with each other in order to motivate people to be physically active in forests. For analysis, Mayring’s qualitative content analysis and a frequency analysis (MaxQDA) were applied. Findings show that most of the interviewed professionals presume forests to have health-fostering effects. Furthermore, something derived from the statements within the context of the thought experiment was that most professionals seem to be willing to cooperate with the other sector. Hence, it might be conceivable that forest exposure may be part of a German stress-coping strategy.  相似文献   

Sorghum extrusion process combined with biofortified sweet potato contributed for high iron bioavailability in Wistar rats     

《Journal of Cereal Science》2017

This study aimed to evaluate the effect of sorghum and sweet potato on the bioavailability of iron, gene expression of proteins involved in iron metabolism and the plasma antioxidant capacity in animals fed with whole sorghum grains processed by dry heat or extrusion, combined or not with sweet potato flour with high content of carotenoids. Five experimental groups were tested (n = 7): dry heat sorghum flour (DS); extruded sorghum flour (ES); whole sorghum flour + sweet potato flour (DS + SP); extruded sorghum flour + sweet potato flour (ES + SP) and positive control (FS). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency, gene expression of divalente metal transporter 1 (DMT-1), duodenal citochroma B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The ES + SP group showed higher (p < 0.05) expression of DcytB, ferroportin and hephaestin when compared to the control group. The DS group showed high (p < 0.05) expression of DMT-1 and the ES showed high mRNA expression of transferrin and ferritin. The changes in the sorghum physicochemical properties from extrusion process reduced the iron and phytate content, and increased the gene expression of proteins involved in iron metabolism, improving iron bioavailability. The combination of sweet potato and sorghum flour (dry or extruded) improved the iron capture and total antioxidant capacity, probably due to the presence of β-carotene and antioxidant compounds.  相似文献   

Combined mutations in five wheat STARCH BRANCHING ENZYME II genes improve resistant starch but affect grain yield and bread-making quality     

《Journal of Cereal Science》2017

Increases in the proportion of amylose in the starch of wheat grains result in higher levels of resistant starch, a fermentable dietary fiber associated with human health benefits. The objective of this study was to assess the effect of combined mutations in five STARCH BRANCHING ENZYME II (SBEII) genes on starch composition, grain yield and bread-making quality in two hexaploid wheat varieties. Significantly higher amylose (∼60%) and resistant starch content (10-fold) was detected in the SBEII mutants than in the wild-type controls. Mutant lines showed a significant decrease in total starch (6%), kernel weight (3%) and total grain yield (6%). Effects of the mutations in bread-making quality included increases in grain hardness, starch damage, water absorption and flour protein content; and reductions in flour extraction, farinograph development and stability times, starch viscosity, and loaf volume. Several traits showed significant interactions between genotypes, varieties, and environments, suggesting that some of the negative impacts of the combined SBEII mutations can be ameliorated by adequate selection of genetic background and growing location. The deployment of wheat varieties with increased resistant starch will likely require economic incentives to compensate growers and millers for the significant reductions detected in grain and flour yields.  相似文献   

Kernel vitreousness and protein content: Relationship,interaction and synergistic effects on durum wheat quality     

《Journal of Cereal Science》2017

Four sets of durum samples were used in this study to further understand the interrelationships among hard vitreous kernels (HVK), protein content, and pigment concentration, with a focus on the interaction and synergistic effects of protein content and vitreousness on durum quality. HVK level increases with higher protein content in the range of 9.5–12.5%, but this relationship is less evident in durum samples with high protein content (12.5–14.5%). Both protein content and kernel vitreousness can significantly affect durum milling quality. White starchy kernels (WSK) in low protein durum have a very detrimental impact on milling and pasta processing quality, but high protein content can mitigate the adverse impact of WSK on durum quality. Although protein content plays a dominant role, higher HVK might contribute positively to pasta firmness. There was no significant difference in yellow pigment content between HVK and WSK. However, pigment loss from semolina to dough was higher for WSK than HVK. Despite the difference in protein content, HVK and WSK have little difference in gluten strength. The monomeric protein was preferentially accumulated in HVK. The glutenin proteins of HVK and WSK were similar in the ratios of 1Bx/1By and HMW/LMW-GS.  相似文献   

脉冲电流烧结Al₂O₃颗粒的广义热弹性分析     

张龙  张晓敏  褚钟祥  彭松 《保鲜与加工》2017,(4):18-23

脉冲电流烧结过程的颈部形成机理,特别是非导电粉末材料,是需要着重研究的核心问题。以非导电Al₂O₃粉末为研究对象,引入L-S（Lord and Shulman）型广义热弹性方程,初步探究烧结初期非导电粉末颈部局部高温形成以及快速烧结机理。利用Comsol Multiphysics模拟得到脉冲电流烧结过程中颗粒内部的温度场和应力场分布以及烧结颈部的化学势和空位浓度变化规律。数值结果表明,热以波的形式在烧结颈部产生叠加,形成局部高温。化学势变化表明：烧结初期表面扩散占主要作用,空位浓度差的突变使烧结颈部产生局部空位浓度梯度,促进烧结颈长过程,缩短烧结时间。  相似文献   

高温下钢管约束型钢混凝土柱的受力性能   总被引：1，自引：0，他引：1  

王卫永  宋柯岩  刘界鹏 《保鲜与加工》2017,(3):58-66

火灾下无防火保护的结构构件温度会迅速上升,从而造成钢材和混凝土的强度明显下降。为了研究火灾下钢管约束型钢混凝土柱的受力性能,考虑火灾下钢管约束型钢混凝土柱的不均匀温度分布及温度对材料力学性能的影响,提出了火灾下受轴心荷载作用的钢管约束型钢混凝土柱承载力的计算方法。利用有限元软件ABAQUS对提出的计算方法进行了验证,结果吻合较好。进而采用该计算方法对影响高温下承载力的参数进行了分析,研究表明：随着构件截面尺寸的增加以及混凝土强度和钢材强度的提高,构件的承载力逐渐增加,而钢管壁厚的改变对承载力并无太大影响。利用有限元软件ABAQUS分析了荷载比、构件尺寸、钢管壁厚等因素对构件耐火极限的影响,发现耐火极限随着荷载比和钢管壁厚的增加而减小,随着构件尺寸的增加而增大。  相似文献   

无核白葡萄多酚氧化酶基因的克隆与序列分析     

刘峰娟  冯作山  白羽嘉  王敏  黄伟  黄文书 《保鲜与加工》2017,17(6):13-19

为获得无核白葡萄中多酚氧化酶(PPO)的基因序列,以无核白葡萄果实为试材,进行基因同源克隆,得到多酚氧化酶基因CDS全长序列,其完整的编码框为1 824 bp,编码607个氨基酸(GenBank登陆号为KP271928);系统进化分析可知,其与葡萄科植物PPO基因同源性最高;生物信息学方法发现该蛋白分子量为67 392.44 Da,等电点为6.42,具典型的酪氨酸家族结构域,无信号肽结构,但有一个跨膜结构域,为亲水性蛋白;二级结构预测发现,PPO具有CuA与CuB两个结合区,分别嵌入蛋白活性腔中。该研究为进一步从分子水平探讨多酚氧化酶与褐变的关系奠定理论基础。  相似文献   

响应面法优化超声波辅助提取鲜切荸荠黄化组织中黄酮类物质工艺研究   总被引：1，自引：0，他引：1  

李长乐  潘永贵 《保鲜与加工》2017,17(4):30-36

以鲜切荸荠为原料,在单因素试验基础上,通过响应面分析法对鲜切荸荠黄化组织中黄酮类物质的提取条件进行优化。结果表明,超声波辅助提取鲜切荸荠黄化组织中黄酮类物质的最佳工艺条件为:液料比30∶1(m L/g),乙醇体积分数60%,提取温度61℃,提取时间16 min,在该条件下得到黄酮提取量为0.539 4 mg·g~(-1),该结果与预测值0.542 1 mg·g~(-1)接近,表明所建模型与实际情况拟合较好,该优化条件可行。  相似文献   

微波对毛酸浆籽油提取效率的影响及其脂肪酸分析     

郝晓磊  张姣姣  李喜宏  刘香军 《保鲜与加工》2017,17(1):99-103

以毛酸浆种子为原料,研究索氏微波提取法对毛酸浆籽油提取效率的影响,并以甲酯化脂肪酸为标准品,采用气相色谱法对毛酸浆籽油脂肪酸成分进行分析,通过与标准品比对出峰时间确定试样脂肪酸成分,使用面积归一法确定试样中各脂肪酸含量。结果表明,与普通热风干燥相比,微波干燥法能够提高毛酸浆籽油的提取效率;利用气相色谱法对毛酸浆籽油进行脂肪酸分析,共检测出8种脂肪酸,分别为:肉豆蔻酸1.3%,棕榈酸10.3%,硬脂酸5.3%,油酸14.3%,亚油酸62.7%,亚麻酸4.0%,花生酸1.1%,二十二烷酸1.0%。其中不饱和脂肪酸相对含量占81.0%,说明毛酸浆籽油具有较高的营养价值,开发前景广阔。  相似文献