Adeno-associated virus type 9-mediated p65 silencing inhibits angiotensin II-induced apoptosis of H9c2 cells     

XING Meng-yao  MA Xiao-juan  GONG Xue-li  Maimaitizunong MAISUER  YU Wen-yan  ZHANG Xue-mei  SUN Zhan 《园艺学报》2018,34(11):2101

AIM: To study the effect of p65 gene silencing by adeno-associated virus type 9 (AAV9)-mediated RNA interference on angiotensin Ⅱ (Ang Ⅱ; 10^-6 mol/L for 24 h)-induced apoptosis of rat ventricular H9c2 myocytes, and to elucidate the possible mechanism. METHODS: The H9c2 cells were transfected with rAAV9-eGFP and rAAV9-eGFP-NF-κB p65-siRNA at multiplicity of infection (MOI)=4×10⁶ vg/cell. eGFP expression in the cells was observed under an inverted fluorescence microscope, and the percentage of eGFP positive cells was determined by flow cytometry. The expression of p65 was determined by Western blot. CCK-8 assay was used to measured the viability of transfected H9c2 cells. The apoptosis of the cells transfected with the virus and with Ang Ⅱ stimulation was analyzed by flow cytometry. RESULTS: The cells began to exhibit eGFP expression on the 2nd day after transfection. The fluorescence intensity was increased over the time of transfection. eGFP expression reached the maximum on the 5th day, and the transfection efficiency was (52.7±1.9)% at this time point. Compared with blank control group, no significant effect of AAV9 on the viability of H9c2 cells was observed. In resting state, p65 in the H9c2 cells had a certain activity. After Ang Ⅱ stimulation, the activity of p65 was obviously increased, while transfection of rAAV9-eGFP-NF-κB p65-siRNA effectively inhibited the expression of p65. The apoptosis of H9c2 cells in Ang Ⅱ stimulation group was significantly higher than that in blank control group, while transfection of rAAV9-eGFP-NF-κB p65-siRNA effectively inhibited apoptosis of H9c2 cells. CONCLUSION: Transfection of rAAV9-eGFP-NF-κB p65-siRNA effectively inhibits the expression of p65 gene of NF-κB pathway in the H9c2 cells without causing cell growth inhibition, and reduces the apoptosis induced by Ang Ⅱ.  相似文献   

Isolation and structural characterization of rhamnogalacturonan II-borate complex fromPinus densiflora     

Tomoko Shimokawa  Tadashi Ishii  Toshiro Matsunaga 《Journal of Wood Science》1999,45(5):435-439

A boron-containing pectic polysaccharide was isolated from Driselase digests of akamatsu (Pinus densiflora) cell wall. The boron-containing polysaccharide was purified by successive column chromatography of Cosmosil C₁₈ OPN, DEAE Sepharose FF, and Superdex 75. The complex had 0.12% (w/w) boron (B) and¹¹B-NMR spectroscopy showed the complex to be the tetrahedral diester form of boron. The sugar composition and glycosyl linkage analyses showed that the sugar portion of the complex was rhamnogalacturonan-II (RG-11). The boron-attached glycosyl residue was identified to be 2-O-Me-xylose-containing apiosyl side chain in RG-II. These results established that the location of borate in the cell wall is conserved among dicots, gramineous monocots, and coniferous gymnosperm.Part of this study was presented at the 8th Cell Wall Symposium, Norwich, September 3, 1998  相似文献   

陆地棉品种与陆地棉族系种质系间杂种优势及其组成分析   总被引：7，自引：1，他引：6  

游俊  刘金兰 《作物学报》1998,24(6):834-839

采用ＮＣⅡ遗传交配设计,选用３个陆地棉常规品种和５个陆地棉族系种质系材料杂交,杂种Ｆ１代产量和纤维品质优势表现明显。通径分析结果表明,提高铃重,单株铃数的中亲优势,适当降低单铃种子数和株高的优势。在其它性状适宜的情况下,可以显著地提高皮棉产量的中亲优势。各个优势组合在产量优势来源上有差异。种质系材料在杂种优势利用上具有很大潜力。  相似文献   

Specific MHC class II B alleles associated with resistance to Edwardsiella tarda in turbot, Psetta maxima (L.)     

J-Y Xu  S-L Chen  H Ding 《Journal of fish diseases》2009,32(7):637-640

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原位合成纳米金/石墨烯修饰玻碳电极检测水和土壤中痕量铅   总被引：2，自引：2，他引：0  

李杜娟  徐枫  樊凯  厉力华  苏畅  刘红英  姚睿  曹佳斐  魏凯华  李小平  赵新颜 《农业工程学报》2018,34(11):203-208

为提高痕量铅的检测精度,提出了一种简单、可控的一步还原法在裸玻碳电极表面原位合成纳米金/石墨烯材料的方法,并通过循环伏安法(cyclic voltammetry,CV)对修饰电极进行电化学表征。利用修饰后的电极对醋酸-醋酸钠缓冲溶液中的铅离子(Pb(Ⅱ))标准样品进行检测,并对检测条件进行了优化。优化条件下的试验结果表明,在pH值为4.5的醋酸-醋酸钠缓冲溶液中,Pb(Ⅱ)的溶出峰为-0.08 V,在1～90μg/L范围内Pb(Ⅱ)的溶出峰电流与Pb(Ⅱ)浓度之间呈现良好的线性关系(R~2=0.985),最小检测下限为0.27μg/L。在优化后的条件下采用该电极测定了实际水样与土壤中的Pb(Ⅱ)含量,加标回收率区间分别为93.75%～109.20%和93.82%～109.9%,相对标准偏差均小于7%,可以用于对实际水样与土壤样本的检测。  相似文献   

Monitoring the photosystem II behavior of wild and cultivated barley in response to progressive water stress and rehydration using OJIP chlorophyll a fluorescence transient     

Ali Akbar Ghotbi-Ravandi  Maryam Shahbazi  Mohammad Pessarakli 《Journal of plant nutrition》2016,39(8):1174-1185

To study the effects of progressive drought stress on photosystem II behavior of wild type (Spantaneum) and cultivated barley (Morocco), different levels of soil water availability, including control, moderate, mild, and severe water stress (70%, 50%, 30%, and 10% water holding capacity of soil, respectively) and rehydration were used. Polyphasic OJIP fluorescence transient of Morocco plants exhibited a considerable increase in fluorescence intensity at O, J, and I steps under mild and severe stress relative to slight increase in wild barley. Values of fluorescence parameters and quantum efficiencies, including minimal fluorescence, relative variable fluorescence at phase J and I, maximal quantum yield of photosystem II (PSII), performance index, electron transport yield, and excitation transfer efficiency were influenced by water stress in both genotypes. These parameters were significantly less affected in wild type barley by progressive drought stress compared to Morocco. After re-watering, both genotypes were able to restore from severe drought in most of the traits. Based on our findings, highly correlated values of relative water content (RWC) and independent JIP-test parameters (P < 0.01) indicate that the chlorophyll a fluorescence induction technique is sensitive to plant water status and performance index represent an accurate and reliable indicator for early stress detection and also explore plant vitality under water stress.  相似文献   

Cryptic species composition and genetic diversity within Bemisia tabaci complex in soybean in India revealed by mtCOI DNA sequence     

Prasanna H C  Kanakala S  Archana K  Jyothsna P  Varma R K  Malathi V G 《农业科学学报》2015,14(9):1786-1795

Bemisia tabaci is a cryptic species complex, causing significant loss on many agriculturally important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving sustainable pest management strategies. Here we investigate the whitefly species complex in soybean in major soybean growing states of India. The mitochondrial cytochrome oxidase gene subunit-1(mt COI) based phylogenetic relationships established using Bayesian methods indicated the existence of three cryptic species namely AsiaⅠ, AsiaⅡ1, and AsiaⅡ7. All the haplotypes detected in the study could be assigned to these three cryptic species following the species demarcation criteria of 3.5% divergence threshold. Of these, AsiaⅡ1 was found to be predominant with wide spread distribution across the surveyed regions from cool temperate zones to hot and humid tropical plains. On the contrary, cryptic species AsiaⅡ7 showed localized distribution. The AsiaⅡ1 exhibited the highest haplotype diversity and AsiaⅠ showed high level of nucleotide diversity. There was a significantly high genetic differentiation among these three cryptic species. The MEAM 1, a dreadful invasive species was not detected in the specimens tested in the current study. The diversity and distribution of three cryptic species is discussed in the light of current knowledge on distribution of whitefly species in India and yellow mosaic disease observed during sampling survey.  相似文献   

Identification of a new PSII target site psbA mutation leading to D1 amino acid Leu218Val exchange in the Chenopodium album D1 protein and comparison to cross‐resistance profiles of known modifications at positions 251 and 264     

Heike Thiel  Mark Varrelmann 《Pest management science》2014,70(2):278-285

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Cross‐resistance risk of the novel complex II inhibitors cyenopyrafen and cyflumetofen in resistant strains of the two‐spotted spider mite Tetranychus urticae     

Mousaalreza Khalighi  Luc Tirry  Thomas Van Leeuwen 《Pest management science》2014,70(3):365-368

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新型钴配合物为中性载体的水杨酸根电极研究     

廖家耀  叶光荣  袁若  柴雅琴 《西南大学学报(自然科学版)》2006,28(3):491-495

研究了基于水杨醛缩邻氨基苯酚合钴(Ⅱ)金属配合物[Co(Ⅱ)-SAAP]为中性载体的PVC膜阴离子电极。该电极对水杨酸根(Sal-)具有优良的电位响应性能和选择性并呈现出反Hofm e ister选择性行为,其选择性次序为Sal->C lO4->SCN->I->NO2->NO3->B r->C l->SO32->SO42-。在pH为5.0的磷酸盐缓冲体系中,电极电位呈现近能斯特响应,线性响应范围为9.0×10-6~1.0×10-1mol/L,斜率为-54.3 mV/dec(20℃),检出下限为5.0×10-6mol/L。采用交流阻抗技术和紫外可见光谱技术研究了电极的响应机理,结果表明配合物中心金属原子的结构以及载体本身的结构与电极的响应行为之间有非常密切的构效关系。该电极具有响应快、重现性好、检出限低、制备简单等优点。将电极用于药品分析,其结果令人满意。  相似文献