Agrobacterium-mediated transformation of the CrDAT gene and selection of transgenic periwinkle lines with a high vincristine accumulation     

Thi Thanh Nhan Pham  Thi Ngoc Lan Nguyen  Thi Ha Bui  Huu Quan Nguyen  Thi Tam Nguyen  Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598

Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg^?1 to 5.12 μg.mg^?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g^?1 (fresh weight) to 10.53 µg.g^?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.

Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter  相似文献   

Spiro N‐methoxy piperidine ring containing aryldiones for the control of sucking insects and mites: discovery of spiropidion     

Michel Muehlebach  Anke Buchholz  Werner Zambach  Juergen Schaetzer  Miriam Daniels  Ottmar Hueter  Daniel P Kloer  Rob Lind  Peter Maienfisch  Andy Pierce  Thomas Pitterna  Tomas Smejkal  David Stafford  Laura Wildsmith 《Pest management science》2020,76(10):3440-3450

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Radioisotope and stable isotope ratios (Δ14C, δ15N) suggest larval lamprey growth is dependent on both fresh and aged organic matter in streams     

Thomas M. Evans  Amber R. Bellamy  James E. Bauer 《Ecology of Freshwater Fish》2019,28(3):365-375

Lampreys have a complex life cycle which includes a multi‐year infaunal larval stage (ammocoete). Gut content analysis has generally identified detritus (i.e., unidentifiable organic matter) as the major dietary component to ammocoetes, though algae can also be important. However, gut content preserves only a snapshot of the animal's diet and does not reflect assimilated material. In order to better characterise the nutritional sources supporting ammocoete growth, we analysed ammocoete body tissue and potential dietary sources at two streams using natural Δ¹⁴C and δ¹⁵N to estimate time‐integrated nutritional support. Bayesian isotope mixing models revealed differences in the importance of sources supporting ammocoetes between sites. Ammocoetes from a stream in a mixed land usage area (~50% agriculture, ~40% forest and ~10% developed) were primarily supported (mean: ~50%) by fresh terrestrial organic matter but were also supported by substantial contributions (mean: ~30%) by aged organic matter (AOM) and autochthonous material (algae; mean ~20%). In a predominantly forested (~90%) headwater stream, different modelling scenarios (uninformed or informed priors) suggested that algal support of ammocoete nutrition ranged from 7% to 45%. However, the model relying on informed priors developed from gut content analysis produced the low estimates, suggesting these were more reliable. When algae were a minor component of the nutrition at the forested site, ammocoetes were highly dependent on AOM (83 ± 26%; mean ± SD). Based on these findings, ammocoete growth and development are predicted to be strongly influenced by both land use and the availability of allochthonous and autochthonous materials of varying ages within streams.  相似文献   

Constructing sustained‐release herbicide formulations based on poly‐3‐hydroxybutyrate and natural materials as a degradable matrix     

Evgeniy G Kiselev  Anatoly N Boyandin  Natalia O Zhila  Svetlana V Prudnikova  Anna A Shumilova  Sergey V Baranovskiy  Ekaterina I Shishatskaya  Sabu Thomas  Tatiana G Volova 《Pest management science》2020,76(5):1772-1785

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Spatiotemporal patterns of mountain whitefish (Prosopium williamsoni) in response to a restoration of longitudinal connectivity          下载免费PDF全文

Peter M. Kiffney  Ben Cram  Paul L. Faulds  Karl Burton  Michele Koehler  Thomas P. Quinn 《Ecology of Freshwater Fish》2018,27(4):1037-1053

To examine the role of longitudinal connectivity on the spatial and temporal dynamics of mountain whitefish (Prosopium williamsoni), we quantified movement and population dynamics following installation of the Landsburg Dam fishway, Cedar River, WA, USA. Mountain whitefish is widely distributed, poorly studied and not the focus of restoration. Before the fishway, mountain whitefish were not observed above the dam. Here, we focus on snorkel counts collected at reach and mesohabitat (e.g. pools) scales over 11 summers on the 20‐km above‐dam segment following restoration. A camera within the ladder provided number, size and movement timing, thereby informing on behaviour and recolonisation. Segment‐scale abundance increased following fish passage reaching an asymptote in 7 years, and mountain whitefish were detected throughout the main stem in 10 years. Annual movement through the ladder increased over time and was positively correlated with instream abundance and discharge, but negatively correlated with water temperature. About 60% of fish movements occurred in spring and early summer, potentially for foraging opportunities. Reach‐scale abundance peaked between 7 and 10 km from the dam; deep, cool (~10.6 to 11.6°C) conditions characterised these reaches. At the mesohabitat scale, mountain whitefish detection increased with depth and velocity after accounting for distance from the dam. Our results show how restoring longitudinal connectivity allowed this nontarget species to colonise newly available habitat. Their response supports the critical roles of longitudinal connectivity and environmental conditions, that manifest at different spatial scales, in dictating how freshwater fish respond to habitat disturbance.  相似文献   

Increased litter size in Rambouillet sheep: I. Estimation of genetic parameters.     

D F Waldron  D L Thomas 《Journal of animal science》1992,70(11):3333-3344

The variance and covariance components needed to estimate heritabilities of and genetic correlations among litter size, ovulation rate, scrotal circumference, and BW in a flock of Rambouillet sheep were estimated using REML via an expectation-maximization type algorithm. The heritability estimates from univariate analyses were .14, .21, .25, .36, and .15 for litter size, ovulation rate, scrotal circumference, 180-d BW of females, and 180-d BW of males, respectively, and average heritability estimates from bivariate analyses were .19, .20, .20, .34, and .10 for litter size, ovulation rate, scrotal circumference, 180-d BW of females, and 180-d BW of males, respectively. The genetic correlation between litter size and ovulation rate was near unity. Body weight in ewes had a moderate genetic correlation with both litter size (.22) and ovulation rate (.20) and a low residual correlation with both litter size (.03) and ovulation rate (.09). The genetic correlation between BW in rams and scrotal circumference was 0, whereas the residual correlation was .71. The genetic correlations of scrotal circumference with litter size and ovulation rate were -.25 and +.20, respectively.  相似文献   

No effect of turkey herpesvirus infections on the outcome of avian pneumovirus infections in turkeys.     

S Van de Zande  H Nauwynck  M Pensaert 《Avian diseases》2001,45(2):517-521

To determine whether turkey herpesvirus (HVT) impairs the aspecific and specific defense against an avian pneumovirus (APV) infection, specific-pathogen-free turkeys were inoculated at 7 days of age with HVT and 1, 5, or 7 wk later with APV. Clinical signs, APV replication, and development of antibodies against APV were evaluated. No differences were found between the birds that received both HVT and APV and those that received only APV.  相似文献   

Management of Critical Care Facilities     

Thomas E. Catanzaro DVM  MHA 《Journal of Veterinary Emergency and Critical Care》1994,4(1):45-47

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Neurological Manifestations of Niemann-Pick Disease Type C in Cats   总被引：3，自引：1，他引：2  

Karen R. Muñana  Patricia J. Luttgen  Mary Anna Thrall  Thomas W. Mitchell  David A. Wenger 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(2):117-121

Seven Domestic shorthair cats with a lysosomal storage disorder analogous to human Niemann-Pick disease type C, from a breeding colony were studied to characterize the neurological manifestations of this disorder. Affected cats were identified by means of liver biopsies at 4 to 6 weeks of age. Neurological examinations were performed at 2 week intervals from the onset of clinical signs. All cats displayed signs referrable to the cerebellum, with a subtle intention tremor noticed initially at 8 to 12 weeks of age; the disease was rapidly progressive. The tremor became more pronounced, menace response was lost, and severe dysmetria and ataxia developed. Three cats also had signs referrable to other areas of the central nervous system. Cats died or were euthanized between 12 and 43 weeks of age. Pathological findings included accumulation of substrate within neurons throughout the central nervous system, and axonal spheroid formation. The clinical and pathological findings in these cats are comparable to those in the human form of the disease.  相似文献   

Growth of triploid oyster, Crassostrea madrasensis (Preston)   总被引：1，自引：0，他引：1  

Jyothi V Mallia  Palaniyappan Muthiah  & Palahani C Thomas 《Aquaculture Research》2006,37(7):718-724

The performance of I and II meiotic triploids and control oysters (Crassostrea madrasensis) reared at Tuticorin Bay was compared to determine if the improvements in the growth of edible oysters were additive to faster growth in triploids. After a grow‐out period of 12 months, both mean whole weights and shell heights were in order I meiotic triploid>II meiotic triploid>control. Mean whole weights and shell height of different oyster lines were all significantly different (P<0.05). On an average, larger morphological traits indicated that growth improvements from triploids were additive, and throughout the study triploid oysters maintained faster growth rate than their diploid siblings. Condition index and adductor muscle diameter of both triploids were higher than those of control.  相似文献