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31.
研究了咪唑类化合物KK—42对家蚕卵巢发育以及蛋白质合成的影响,结果表明KK-42对卵巢增重有明显的抑制作用,血淋巴蛋白含量在蛹期变化幅度很小,卵巢蛋白质含量增加也很缓慢;而对照区在化蛹后,血淋巴蛋白急剧增加,第三天达到高峰,以后又迅速下降,与卵巢蛋白质增加相吻合。由此表明,咪唑类化合物KK-42不仅抑制血淋巴蛋质的合成,同时还抑制了血淋巴蛋白向卵巢的转移。 相似文献
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Dietary protein for young grazing sheep: Interactions with gastrointestinal parasitism 总被引:1,自引:0,他引:1
Effects of protein supplementation and of nematode control on production responses in young grazing sheep and on nematode population dynamics were assessed. Young Merino wether sheep (n = 270) were allocated to one of three supplementation (Su) treatments and one of three regimes of nematode control (drench, Dr) in a factorial design. Each of the nine treatments was replicated three times in a randomised complete block. Animals received no supplement (SuO) or were given supplements at a level equivalent to 200 g day−1, fed three times a week. The supplements contained 25% lucerne meal with either 75% sunflower meal (Su1) or 75% formaldehyde-treated sunflower meal (Su2). Nematode control regimes were: not drenched unless survival of individual animals was threatened (DrO); drenched according to a strategic drenching programme (Dr1); treatment with a controlled-release albendazole capsule (Dr2). Infections with Haemonchus contortus were suppressed by use of closantel. Due to drought conditions and lack of pasture growth, lucerne hay was fed to all sheep from Week 14 onwards (350 g per head per day, fed twice a week). Supplementation reduced the need for ‘survival drenching’ in Dr0 sheep considerably. Live-weight gain was increased significantly by supplementation with Su1 or Su2 during the 36-week experiment. Undrenched animals given Su1 or Su2 tended to grow faster than unsupplemented animals in the Dr1 and Dr2 groups. Greasy wool production and fibre diameter were increased by supplementation and anthelmintic treatment. Faecal worm egg counts (FEC) in undrenched sheep were significantly lower for Su1 and Su2 sheep than for Su0 sheep. There were no effects of supplementation on FEC in Dr1 or Dr2 sheep. Worm burden was generally unaffected by supplementation, while the drenching treatments Dr1 and Dr2 reduced worm burden at Week 16 and to a lesser extent at Week 27. Trichostrongylus colubriformis was the predominant nematode species, with smaller numbers of Nematodirus spp., Trichostrongylus vitrinus, Trichostrongylus axei and Ostertagia circumcincta also present. It was concluded that supplementary feeding with protein meal substantially reduced production losses attributable to nematode infections in young grazing sheep. This appears to be due to an enhanced resilience of the host, rather than any major changes in development of protective immunity. 相似文献
34.
Hagemoser WA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1989,18(1):17-20
An intravenous bile acid loading test was performed on 16 goats using various dosages and sampling protocols. A recently developed enzymatic bile acid assay provided an economical and technologically simple method for bile acid analysis. Intravenous injection of bile salts into goats was well tolerated. Clearance times were rapid. A protocol for performance of the test was suggested and a practical reference range for T 1/2 clearance of 4+/-1 minute was determined. Further investigations of this test in the assessment of liver function in the ruminant appear warranted. 相似文献
35.
Blood samples were collected every 2 h during a 24 h period from 6 cows of one herd and 10 cows of another herd. In a third herd 9 cows were sampled every 2 h from 6 a.m. to 8 p.m. Concentrations of total bile acids, acetoacetate, glucose and free fatty acids were determined in blood plasma. A marked difference in individual bile acid concentrations and patterns of diurnal variation was found. For most cows the highest bile acid values were observed between 2 and 6 a.m. (overall mean (+/- SD) at 6 a.m.: 104 +/- 84 mumol/l, range: 20-307 mumol/l). Fourteen cows with a bile acid value greater than 90 mumol/l at 6 a.m. ("high BA") were characterized as a group by showing a pronounced decrease in the mean bile acid concentration after morning feeding. In the group of 11 cows with a 6 a.m. bile acid value less than 90 mumol/l ("low BA") the time of day did not contribute significantly to the bile acid variation. For the "high BA" group a nearly synchronous variation between the mean values of the 3 feeding dependent parameters (acetoacetate, glucose and free fatty acids) and the mean values of bile acids was found. The within animal coefficients of correlation between bile acids and the feeding dependent parameters were significantly higher in the "high BA" group than in the "low BA" group. No direct connection was found between bile acid levels and the quantity of concentrates fed or the individual milk yield. 相似文献
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AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer. 相似文献
39.
AIM:To observe the dynamic changes of expression of PKCα, TGF-β1 and α-SMA in glomeruli of diabetic rats induced by the alloxon and to invesitigate their roles in the diabetic nephropathy(DN).METHODS:Rats were randomly divided into four groups: normal control group (group A), diabetic group of one week (group B), diabetic group of one month (group C), diabetic group of two months (group D). Immunohistochemistry and Western blotting were used to detect the expression of PKCα, TGF-β1 and α-SMA in renal tissue of all groups. Blood glucose, triglycerides, cholesterol, creatinine and urine protein were analysed by chemical methods. The morphological changes of renal tissue were checked through microscopy.RESULTS:The expression of PKCα and TGF-β1 in renal tissue of diabetic groups were increased comparing with those of nomal control group(P<0.05). The mesangial cells expressed α-SMA in two months group. Chronologically the expression of PKCα, TGF-β1 and α-SMA were positively correlative with each other and the impairment of kidney was also observed.CONCLUSIONS: During the DN process the expression of PKCα increased. PKCα raised GFR and the permeability of glomerular filtration membrane which enhanced urinary albumin excretion. PKCα also increased expression of TGF-β and therefore to induce the expression of α-SMA. The appearance of α-SMA was a marker of the phenotypic transform of renal cells. 相似文献
40.
AIM:To explore the expressive profile of nestin protein in the focal ischemic brain and to study the recovery mechanism of brain focal infarct.METHODS:Cellular morphology,time-course and distribution pattern of nestin positive response were immunohistochemically examined in different brain regions of 36 adult male SD rats. RESULTS:Nestin positive response of different brain regions in sham operated rats was present in small- and micro-vasculartures and the third ventricle bottom and ependyma. A large number of nestin positive cells were detected in ischemic brain, and were more remarkable in the cortical areas of parietal lobe and preoptic area as well as ischemic caudoputamen. Stellate nestin positive cells were located in the deep layer of ischemic cortex, but fibrillary cells were located in the shallow layer. Nestin positive cells in the ischemic caudoputamen showed the same changes of morphology as those cells in the deep layer of ischemic cortex. Morphological and number alterations of nestin positive cells were the most remarkable at 1 weeks post-ischemia, which showed more hypertrophy and proliferation in morphology, and a marked increase in number was present in the ischemic cerebral cortex and the ischemic caudoputamen. These alterations of nestin positive cells persisted up to 6 weeks post-ischemia, and then, the nestin positive response in the ischemic brain decreased gradually.CONCLUSION:Focal cerebral ischemia induces nestin re-expression on reactive astrocytes, which may be very important to the self-recovery of cerebral infarct. 相似文献