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The aim of this study was to evaluate the contribution of three plasma antioxidants (albumin, uric acid, SH groups) to the plasma total peroxyl radical-trapping antioxidant capacity (TRAP) in 2 and 4 h of intestinal reperfusion in rats. TRAP increased significantly both after 2 and 4 h of reperfusion. Neither albumin nor SH groups contributed significantly to this increase. TRAP was strongly influenced by the increase in uric acid concentration and also probably by the cell destruction caused by oxidative stress. Since the TRAP increase was accompanied by an increase in the level of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation), we can conclude that even such a large increase in TRAP is not sufficient to prevent the progression of lipid peroxidation and oxidative stress. 相似文献
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K. Kow S. M. Bailey E. S. Williams S. Withrow S. E. Lana 《Veterinary and comparative oncology》2006,4(3):184-187
Appendicular osteosarcoma (OSA) is the most common primary bone tumour in dogs, and the prognosis with standard of care therapy of amputation and adjunctive chemotherapy is generally poor, with median survival times of 1 year. The ability of neoplastic cells to maintain their telomere length, by either telomerase activity or alternate methods, is an important step in tumour development and malignancy. The purpose of this study was to determine the presence of telomerase activity in canine OSA. To evaluate the frequency of alternative lengthening of telomeres in canine OSA, we have used the telomeric repeat amplification protocol in five canine cell lines and in six samples taken from clinical patients at the time of amputation. Our results reveal the presence of telomerase activity in 100% of canine OSA cell lines and 83% of clinical samples evaluated. This is in contrast to human OSA where 25–40% expression levels of telomerase are reported. Importantly, our results not only suggest that canine OSA may serve as a good model for aggressive telomerase‐positive forms of human OSA but also that antitelomerase therapy strategies for treatment of canine OSA may be more successful than in the treatment of majority of human patients with OSA. 相似文献
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Kow K Thamm DH Terry J Grunerud K Bailey SM Withrow SJ Lana SE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2008,22(6):1366-1372
Background: We demonstrated previously that canine osteosarcoma (OSA) cell lines and samples from clinical patients are predominantly telomerase positive. In contrast, the majority of OSA samples from human patients appear to be telomerase negative, maintaining telomere length by an alternative lengthening of telomeres (ALT) mechanism. The purpose of the current study was to examine the telomerase status of a large number of OSA samples from dogs and determine if telomerase status can serve as a prognostic factor. Hypothesis: The majority of clinical canine OSA appendicular lesions will be telomerase positive, and telomerase positivity will negatively impact disease outcome. Animals: Sixty‐seven dogs with appendicular OSA presenting to the Colorado State University Animal Cancer Center for treatment. Methods: The Telomeric Repeat Amplification Protocol was performed on tissue samples from primary canine appendicular OSA to determine the presence of telomerase activity. Telomere restriction fragment (TRF) analysis was utilized to determine telomere length and detect ALT. Outcome data were obtained in a retrospective manner and correlated with telomerase status. Results: Seventy‐three percent of canine OSA samples were telomerase positive. Telomerase status did not have an impact on disease‐free interval or survival time. Nine of 10 telomerase‐negative samples examined were consistent with an ALT phenotype, based on TRF analysis. Conclusions and Clinical Importance: These results are consistent with the hypothesis that the majority of canine OSA are telomerase positive, suggesting that telomerase may be a valuable target for canine OSA therapy. Additionally, telomerase status does not appear to be a prognostic factor in canine OSA. 相似文献
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【目的】获得与Lr24基因紧密连锁并可能作为探针的TRAP分子标记。【方法】应用TRAP技术,选用90对引物组合对小麦抗叶锈病基因Lr24、感病亲本Thatcher及其F2代抗感各10株组成的抗、感基因池（Br、Bs）的扩增带型差异进行分析,用筛选获得的多态性引物对TcLr24×Thatcher F2群体进一步筛选,进而用获得的特异引物对45个小麦抗叶锈病近等基因系和30个小麦二倍体材料进行分析。【结果】获得10对能够在TcLr24、Br与Thatcher、Bs间产生多态性的引物,多态性引物检出率为11.11%。其中1对在F2抗感群体中有差异且稳定扩增的TRAP引物ARBI1/RGA-2F,其161bp 扩增产物仅在F2抗病单株中出现,感病单株中缺失。用该引物对45个近等基因系和30个二倍体材料检测发现,近等基因系TcLr19、TcLr29、TcLr38、Lr42和TcLr44中有相同大小片段的扩增产物,30个二倍体材料中未出现相应扩增产物。【结论】本研究获得的一个与Lr24紧密连锁的TRAP标记,该标记可筛选含有Lr24基因的育种后代群体,可作为探针用于文库的筛选。 相似文献
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参考Genbank已经发表的关于金黄色葡萄球菌TRAP蛋白的基因序列,设计合成1对引物,从本实验室保存的金黄色葡萄球菌Newman株中提取细菌DNA,对TRAP蛋白基因进行PCR扩增,产物经琼脂糖凝胶电泳分析,呈现1条约500bp的条带,回收纯化后,将其克隆至pMD18-T质粒载体中,进行核苷酸序列分析,然后与报道的TRAP蛋白基因进行比较。结果表明:所扩增的基因序列与报道的TRAP核苷酸的同源性高达100/,证实为金黄色葡萄球菌Newman株TRAP蛋白基因。 相似文献
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建立了鲶Silurus asotus的TRAP—PCR反应体系,采用24个TRAP引物组合对鲶人工选育群体进行引物适用性的研究,将获得的多态性位点用于研究鲶群体的遗传结构。结果表明：有9个引物组合可产生有效位点,其中多态引物占所筛选引物总数的37．5％,平均每条引物扩增出15个位点。共扩增出135个位点,多态位点数为75,多态位点比例为55．56％。说明TRAP标记技术适用于鲶的遗传多样性研究,可以作为构建鲶遗传连锁图谱的分子标记。 相似文献
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运用TRAP标记技术,选取10个多态性较好的引物组合对荷包红鲤、黄河鲤、建鲤、兴国红鲤和黑龙江野鲤等5个鲤群体进行遗传多样性分析,其中固定引物是根据目标候选基因GHR基因的序列设计。结果表明,共扩增出168个位点,其中多态性位点134个,平均多态位点比例为80.41%,平均多态性信息含量为0.29。分子变异方差分析(AMOVA)结果显示群体内的方差贡献率达96.97%,表明各个鲤群体内存在较大的遗传变异。种群再分效应固定指数(FST=0.030 26,P<0.05)表明不同鲤群体间有显著的遗传分化。基于目标候选基因的TRAP标记对5个鲤群体进行聚类分析,结果表明建鲤和兴国红鲤首先聚成一类,然后黄河鲤和黑龙江野鲤聚为一类,再与荷包红鲤聚为一类。 相似文献
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