辣椒叶片RNA提取方法研究   总被引：2，自引：2，他引：0  

王燕华  林明  何水林  陈桂信  郭丽红 《福建农林大学学报(自然科学版)》2004,33(3):336-338

以经过紫外线诱导处理的辣椒叶片为材料,分别用Trizol试剂快速提取法、尿素提取法和酚 SDS提取法提取其总RNA,比较各RNA产率、纯度及电泳图谱等,结果表明,尿素法提取的RNA28S和18S条带较为清晰,还可得到23S和16S带,较少有降解;而另2种方法所获得的RNA纯度较低,降解严重,琼脂糖电泳图谱通常只出现1条带.  相似文献   

3种提取基因组DNA方法的比较分析     

刘燕  刘孟洲  徐彦祥 《中国畜牧兽医》2008,35(4):45-47

分别采用3种不同的方法提取基因组DNA，比较所提取的DNA的质量以及提取所需时间、费用，以期选择一种高效、简洁快速、价格合理的提取方法。结果发现这3种方法提取的质量都能达到相关分子生物学试验的要求，但在操作步骤、时间、价格等方面存在差异，具体比较结果可为分子生物学试验选择提取方法提供依据。  相似文献   

Comparison of sampling and isolation procedures for recovery of Yersinia enterocolitica serotype O:3 from the oral cavity of slaughter pigs   总被引：2，自引：0，他引：2  

T Nesbakken 《Acta veterinaria Scandinavica》1985,26(1):127-135

Yersinia enterocolitica serotype 0:3/biotype 4 was isolated from the oral cavity of altogether 32 (68.1 %) of 47 freshly eviscerated slaughter pigs. Most efficient recovery was achieved by cultivation of tissue samples from both tongue and tonsils of the same individual. The isolation rate so obtained was significantly higher than that obtained by separate examination of either tonsil swabs or tongue swabs. However, the isolation frequency achieved by combined swabbing of the 2 sites was not significantly different. In general, tonsils were more productive for the recovery of 0:3 strains than were tongues, and tissue samples yielded higher isolation rates than did swabs. Three-week cold enrichment in a low selective medium proved essential for optimal recovery. However, the highest number of isolates was obtained using a combination of methods, including direct plating and selective enrichment in a modified Rappaport broth in addition to cold enrichment.  相似文献   

Direct non-thermal plasma treatment for the sanitation of fresh corn salad leaves: Evaluation of physical and physiological effects and antimicrobial efficacy     

《Postharvest Biology and Technology》2013

Current problems with outbreaks of serious infections caused by human pathogens on fresh-cut greens highlight the need for new, optimized postharvest sanitation treatments to effectively meet recent food safety standards. In contrast to various chemical treatments, non-thermal plasma (NTP) has a high potential as a gentle target sanitation technique. However, possible interactions between NTP and the physiology of treated fresh products have not been investigated in detail. Here, chlorophyll fluorescence image analysis (CFIA) was used to study the potential impacts of non-thermal plasma on the photosynthetic activity of highly perishable corn salad leaves as a model produce. For this purpose, an atmospheric pressure plasma jet, driven at radio frequency, and transforming argon with flow rates of 20 L min⁻¹ into non-thermal plasma at 10, 20, 30, and 40 W generator power was applied for various times to the surface of corn salad leaves. Thermographic measurements indicated maximum temperatures of 39.0 °C, 44.4 °C, 60.1 °C, and 66.0 °C, respectively, on treated leaf surfaces. CFIA revealed that treatment at moderate generator power of 20 W for up to 1 min was the maximum setting for quality retention. Furthermore, the microbial inactivation efficiency of the plasma jet system at these operating parameters was successfully tested on Escherichia coli bacteria, inoculated on corn salad surface at 10⁷ cfu cm⁻² and 10⁴ cfu cm⁻². At 20 W, bacteria with lower initial load could be inactivated by 3.6 (±0.6) log-cycles within 15 s of treatment duration; whereas at the higher initial load of 10⁷ cfu cm⁻², bacteria were reduced by 2.1 (±0.2) log-cycles after 30 s.  相似文献   

固相萃取——高效液相色谱法分离红花中的黄酮类物质     

张朋美  赵娜  韩黎黎 《张家口农专学报》2008,(4):20-21

目的 研究红花中的黄酮类物质。方法 乙醇浸泡提取，固相萃取分离，液相色谱法进行纯度检测，结果 采用的红花中有两种黄酮类物质。结论 洗脱液V（H2O）：V（C4H5OH）=3：7，能很好的分离红花中的两种黄酮类物质。  相似文献   

不同肥料配比对糜子产量和品质的调控效应          下载免费PDF全文

王君杰  陈凌  田翔  乔治军 《西北农业学报》2024,(1):48-58

为探明不同施肥方式对糜子营养品质、食味品质和产量的影响，于2019年在山西河曲进行试验，以河曲大红糜子为材料，选用不同配比的有机肥（羊粪）、生物菌肥、尿素和复合肥，研究不同施肥配比对糜子品质和产量的影响。结果表明：不同肥料配比对糜子产量和品质性状显著影响，复合肥和有机肥（F+Y）配施显著提高糜子籽粒产量（GY）、净光合速率（P_n）、钙（Ca）和铁（Fe）含量，显著减低直链淀粉含量（AC）；相关性分析发现，籽粒产量（GY）和净光合速率（P_n）呈极显著正相关，相关系数为0.82，镁（Mg）与铁（Fe）呈极显著正相关，值为0.68，直链淀粉含量（AC）与醇溶蛋白含量（Gli）、钙（Ca）达到极显著负相关，值分别为-0.66和 -0.50；主成分分析发现，糜子各性状指标分为4个主成分，分别代表糜子食味品质、营养品质、产量指标和蛋白营养品质，综合得分以复合肥和有机肥（F+Y）处理最高，并显著高于其他处理。综上所述，复合肥（187.5 kg·hm^-2）与有机肥（1.5×10 kg·hm^-2）配施是糜子获得优质高产的主要施肥方式。  相似文献   

预处理对SFE提取月见草种子、被孢霉菌丝体油脂的影响     

孙爱东  葛毅强  蔡同一 《中国农业大学学报》1998,(Z3)

以月见草种子、被孢霉菌丝体为超临界流体萃取(SFE)的试验材料,研究了不同预处理条件(水分、粉碎度、粉碎方式)在不同萃取压力下对超临界流体萃取油脂得率的影响;确定粉碎度、粉碎方式、水分与油脂萃取之间的关系。  相似文献   

孑遗植物水松（Glyptostrobus pensilis）数量性状间的相关聚类分析     

吴则焰  ;刘金福  ;洪伟  ;郑世群  ;陈金英 《吉林林学院学报》2009,(4):358-361

以屏南水松天然林为研究对象，运用尺型聚类分析方法对水松种群的25个数量性状进行聚类分析，探讨水松数量性状间的相互关系及其对水松生长、遗传及生态经济性状的影响．结果表明：水松材积与树高、冠长、胸径等性状呈显著正相关，生长性状与光合作用的面积有关；树高是决定材积的第一要素；水松在干形遗传、自然整枝等方面的遗传性较为稳定．  相似文献   

常用软件绘制使用林地可行性报告附图方法的比较   总被引：1，自引：0，他引：1  

盛俐 《林业资源管理》2007,(6):89-92

使用林地可行性报告的附图是林业专题图类型之一。该类图纸是项目成果重要的组成部分,对文字内容具有补充和说明等作用。本文以一个简单的实例,比较了几类常用软件绘制专题图的各自特点,并简介了综合使用的方法。  相似文献   

利用形状因子法计算木材干燥窑壳体的散热损失     

林金春  杨文斌 《东北林业大学学报》2009,37(4)

指出了木材干燥窑壳体传热量的传统计算方法中的不足,并利用形状因子法来分析计算木材干燥窑壳体的传热量.对比2种计算方法所得的结果表明,传统的干燥窑壳体计算方法较为保守,而根据形状因子法计算结果来配置干燥窑的加热器面积则不会造成干燥窑中散热设备投资的浪费,同时也可以节省干燥窑墙体设计用材的量.  相似文献