基于框架理论的变电站操作票自动生成系统   总被引：3，自引：0，他引：3  

向铁元  谢锋  龚亚峰 《中国农村水利水电》2003,(8):78-81

介绍了一种基于框架理论的变电站操作票自动生成系统。该系统用框架知识表示法来描述电力系统的设备参数，建构了一个直观的、层次分明的数据结构。通过融合框架理论于面向对象的语言VC 中，开发出了一个集通用、实用、智能于一体的操作票专家系统。  相似文献   

Mitochondrial Genome Architecture and Evolutionary Origin of the Yao Silkworm,a Living Fossil of the Domestic Silkworm Bombyx mori (Lepidoptera: Bombycidae)     

Gui-Zheng Zhang  Yu-Li Zhang  Wei Wei  Yu-Ping Li  Yan-Qun Liu  Li-Hui Bi  Cheng Lu 《Journal of insect science (Online)》2022,22(2)

The Yao silkworm is a unique silkworm resource producing yellow flat plate silk that has only been reared by the Baiku Yao ethnic group in Nandan County, Guangxi Province, China for a thousand years. Here, we report the mitochondrial genomes (mitogenomes) of five Yao silkworm strains and 10 local Guangxi strains of the domestic silkworm (Bombyx mori) L. (Lepidoptera: Bombycidae), and use the resulting mitogenomes and the available Bombyx mitogenomes to characterize their genome architecture and trace the evolutionary origin of the Yao silkworm. The five Yao silkworm mitogenomes exhibited genome architectures identical to typical set of 37 mitochondrial genes (13 protein-coding genes, 22 transfer RNAs, and two ribosomal RNAs) and a high level of genome sequence similarity with the domestic silkworm. Mitogenome-based phylogenetic reconstruction provided solid evidence that the Yao silkworm shares a common ancestor with the domestic silkworm. Sliding window analysis uncovered a distinct variation pattern in the mitogenome between the Yao silkworm and the other domestic silkworm strains. The phylogenetic analyses revealed a basal placement of the Yao silkworm among all available domestic silkworm strains, indicating that the Yao silkworm is an ancient population of the domestic silkworm. Our data indicated that the Yao silkworm (B. mori) is a lineage of the domestic silkworm, which for the first time provides insights into the origin of the Yao silkworm.  相似文献   

A Simple and Reliable Assay for Detecting Specific Nucleotide Sequences in Plants Using Optical Thin-film Biosensor Chips     

Here we report the adaptation  optimization of an efficient  accurate  inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified 《分子植物育种》2007,5(2):241-241

Here we report the adaptation and optimization of an efficient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified （GM） crops and SN-P markers in model plant genomes. Briefly, aldehyde-attached sequence-specific singlestranded oligonucleotide probes are arrayed and covalently attached to a hydrazine-derivatized biosensor chip surface. Unique DNA sequences （or genes） are detected by hybridizing biotinylated PCR amplicons of the DNA sequences to probes on the chip surface. In the SN-P assay, target sequences （PCR amplicons） are hybridized in the presence of a mixture of biotinylated detector probes and a thermostable DNA ligase. Only perfect matches between the probe and target sequences, but not those with even a single nucleotide mismatch, can be covalently fixed on the chip surface. In both cases, the presence of specific target sequences is siL, nified by a color change on the chip surface （gold to blue/purple） after brief incubation with an anti-biotin IgG horseradish peroxidase （HRP） to generate a precipitable product from an HRP substrate.  相似文献   

Complete Genome Sequencing and Analysis of Rehmannia Mosaic Virus Isolate from Shanxi Province     

Wang De-fu  Zhang Xi-mei  Guo Shang  Shen Shao-fei  Long Dan-dan  Li Ling-yu  Niu Yan-bing 《东北农业大学学报(英文版)》2021,28(3):58-65

Using double-stranded RNA(dsRNA) technology and sequence-independent amplification(SIA), the molecular identification on infected Rehmannia glutinosa in the field with mosaic symptoms was performed and the whole-genome of the Rehmannia mosaic virus(ReMV) Shanxi isolate(ReMV-SX) was sequenced. Sequencing analysis showed that the virus that infected Rehmannia glutinosa was Rehmannia mosaic virus(ReMV). The full-length of the obtained ReMV-SX sequence(GenBank accession no. JX575184) was 6 395 nt, containing four open reading frames(ORFs). The sequence homology analysis of the complete nucleotide sequence showed that ReMV-SX was 93.8%-97.0% homologous to ReMV in Tobamovirus subgroup I, while only 49.8%-58.9% homologous to the isolates in subgroups II and III of the same genus. Phylogenetic analysis showed that ReMV-SX and ReMV-Henan formed a separate branch and had the closest genetic relationship. The results laid the foundation for ongoing researches in the taxonomic status and evolution of ReMV and for further investigating the pathogenic mechanism of ReMV infecting Rehmannia glutinosa.  相似文献   

黄河小浪底库区不同人工林水土保持效益研究   总被引：1，自引：0，他引：1  

李晓晨  高喜荣  赵东 《河南林业科技》2007,27(3):12-14

采用空间代替时间的方法,通过对黄河小浪底库区不同人工林水土保持效益浅要分析,结果表明:最大吸水率为刺槐>侧柏>20多年生栓皮栎>40多年生栓皮栎,凋落物储量和最大持水量均为20多年生栓皮栎>40多年生栓皮栎>侧柏>刺槐;土壤渗透性能和贮水性能变化较为复杂,侧柏林地的渗透性和贮水性能较其它林地好些,刺槐林地上下层之间变化最大。  相似文献   

猪泛素C端水解酶L1基因的克隆与序列分析   总被引：1，自引：0，他引：1  

田兴国  李加琪  陈瑶生  高萍  梅盈洁  凌飞  罗永发 《华南农业大学学报》2007,28(1):100-104

从人泛素C端水解酶L1(UCH_L1)基因出发,在dbEST数据库中同源搜索,找到1条与人UCH_L1基因编码氨基酸同源性较高且在香猪背最长肌中表达的EST(BM194679).通过电子克隆和进一步RT-PCR试验验证,获得猪UCH_L1基因全长cDNA序列,其全长 1 105 bp,开放阅读框(ORF)位于60～728 bp,编码223个氨基酸.同源性分析结果表明,与人、鼠UCH_L1基因cDNA编码区(CDS)同源性分别为91.2%和86.5%,蛋白质序列同源性均为96.6%.对该基因编码蛋白的结构和功能预测显示含有2个典型的疏水性区域,不含有信号肽,存在1个UCH_L1(pfam01088)保守结构域和多个磷酸化位点,属UCH_L1蛋白家族,故将该基因命名为猪泛素C端水解酶L1基因(登录号AY495532).  相似文献   

植物EST-SSR标记开发及其应用     

张利达  唐克轩 《广西农业生物科学》2010,(3):534-541

随着生物科技的进步,大量的植物表达序列标签（expressed sequence tags,ESTs）已经成为开发SSR标记的重要资源。EST-SSR作为一种新型分子标记,其多态性可能与基因功能直接相关,而且在相近植物间具有良好通用性,使得EST-SSR标记在实际应用中更具价值。采用计算机方法大规模发掘EST-SSR多态性位点极大地提高了SSR标记开发效率。尤其随着新一代测序技术的成熟以及测序成本的急剧下降,利用新一代测序技术产生大量的转录组数据进行EST-SSR多态性标记的计算机大规模发掘将对SSR标记的开发带来深远影响。本文简要介绍了植物EST-SSR分布特点,并对EST-SSR标记开发现状以及相关应用作了综合评述,此外,还对EST-SSR标记的计算机开发新策略进行了展望。  相似文献   

基于B/S模式的实验室预约系统UML建模与研究     

吐尔逊．买买提  董丹华 《计算机与农业》2010,(4):29-31,42

UML作为一种面向对象的标准建模语言,在信息管理系统的建模领域得到了广泛的应用。概述了UML中多种模型图的使用方法和适用范围,重点分析了UML系统建模的主要过程和特点,并以一个实验预约系统为例详细介绍了系统用例模型、静态模型和动态模型的设计过程。  相似文献   

水稻南方黑条矮缩病毒湖南鼎城株系S10片段的基因组序列分析     

崔亚  朱俊子  周倩  蒋坚  高必达 《广西农业生物科学》2012,31(2):160-166

运用RT-PCR技术克隆了水稻南方黑条矮缩病毒（southern rice black-streaked dwarf virus,SRBSDV）湖南鼎城株系的基因组S10片段（SRBSDV-HuNDCS10）,并对其全序列进行了测定和生物信息学分析。结果显示,SRBSDV-HuNDC S10片段全长为1797bp（登录号：JQ337964）,含有1个ORF,编码557个氨基酸残基的衣壳蛋白,推测分子量约62.6kD,推测等电点为7.62,与已报道的广东、海南和云南分离物病毒的S10作比较,它们的核苷酸相似性分别为99.7%、99.0%和98.4%,氨基酸相似性分别为100.0%、99.5%和99.3%。对SRBSDV-HuNDCS10及部分Fijiviruses病毒对应片段在5＇URT与3＇URT存在的保守序列和互补序列进行了归纳,对其ORF编码的氨基酸序列进行了motif查找,得到该属（Fijiviruses）氨基酸序列的10个保守区段。此外,进行了糖基化位点、磷酸化位点及B细胞抗原表位预测,发现了3个可能的N端豆蔻酰基化位点,可能与病毒的侵染机制有关。  相似文献   

基于窗口观测的电控柴油机判缸控制的优化分析     

冒晓建  韦雄  宋君花  王俊席  唐航波  蒋祖华 《农业工程学报》2014,30(15):62-67

柴油机起动初期控制时序的建立过程(判缸控制)直接决定其起动过程的长短,判缸控制是电控柴油机管理系统中最核心的部分。为了缩短判缸时间,提高控制系统的可靠性,该文根据目标柴油机的发火顺序设计了电控系统的转速信号与相位,采用了2套转速计算装置;基于有限状态机,提出了窗口观测联合判缸方法,并开发了电子控制单元(electronic control unit,ECU)复杂驱动层的联合判缸驱动控制软件。试验结果表明该策略是有效可行的,发动机的起动成功率提升10%,达到100%,正常起动成功的时间由1.5 s缩短到1 s以内,改善了电控柴油机的起动性能。该控制策略的实现完善了电控柴油机的时序控制功能,提高了柴油机的电控水平。  相似文献