Antiproliferative Activity and Potential Mechanism of Marine-Sourced Streptoglutarimide H against Lung Cancer Cells     

Hengju Ge  Di Zhang  Muran Shi  Xiaoyuan Lian  Zhizhen Zhang 《Marine drugs》2021,19(2)

  相似文献   

甜菜霜霉病菌形态鉴定及28S rDNA序列分析   总被引：1，自引：1，他引：0  

乾义柯  张祥林  克衣木  陆平 《新疆农业科学》2012,49(2):249-254

[目的]利用形态学和分子生物学技术,对新疆甜菜霜霉病进行鉴定和基因序列分析.[方法]挑取甜菜叶片上霉层,对孢囊梗及孢子囊进行显微观察;提取甜菜霜霉病菌DNA,用卵菌纲通用引物NL1/NL4进行PCR扩增、序列测定、同源性比较.[结果]显微观察到的病原菌孢囊梗及孢子囊与已报道的甜菜霜霉病菌形态一致;PCR扩增28S rDNA末端序列为801 bp,Genbank比对结果显示该段序列与霜霉属粉霜霉菌(Peronospora farinose)最为接近,同源率在99;以上,与霜霉属主要典型株系同源性均在90;以上.[结论]显微形态观察和分子生物学鉴定结果一致,该病原菌属霜霉属,粉霜霉.  相似文献   

Effects of 28% Protein Diets Differing in Quality on Channel Catfish Performance,Processing Traits,and Production Economics in a Multi-Batch System     

Carole Engle  Ganesh Kumar  Rebecca Lochmann  Brian Bosworth  Miles Thompson 《Journal Of Applied Aquaculture》2013,25(2):102-112

Three 28% protein feeds (premium, standard, or a low-cost diet) were fed to channel catfish in a pond study to compare growth, production, and costs as a follow up to similar work with 32% protein feeds. Twelve 0.1-ha earthen ponds at the University of Arkansas at Pine Bluff (UAPB) Aquaculture Research Station were stocked with carryover fish (0.39 kg mean weight) at 5,860 fish/ha and fingerling catfish (mean individual weight of 15.4 g) at 12,500 fish/ha. Total yield (gross and net) did not differ between fish fed the premium as compared to the standard diet, but that of the premium diet was significantly higher than that of fish fed the low-cost diet. The feed conversion ratio (FCR) did not differ between fish fed the premium and standard diets, but was lower than the FCR of fish fed the low-cost diet. There were no differences found in processing yields among treatments. Given the lower cost of the 28% standard diet and yields equivalent to those of the 28% premium diet, the 28% standard diet was economically preferable to the other diets tested.  相似文献   

3株柔嫩艾美耳球虫28SrRNA基因部分序列的扩增与分析     

侯杰  林瑞庆  刘丽丹  陈俊嘉  翁亚彪 《中国畜牧兽医》2012,39(7):43-46

本研究应用PCR技术扩增来自广东的3株柔嫩艾美耳球虫的28S rRNA基因部分序列,并与GenBank^TM登录的柔嫩艾美耳球虫、堆型艾美耳球虫、鼠肉孢子虫和刚地弓形虫虫株的相应序列进行比对分析。试验结果显示,柔嫩艾美耳球虫3个样品均获得1172 bp的28S rRNA基因部分有效序列,不同虫株序列没有差异,与GenBank^TM登录的柔嫩艾美耳球虫相应序列只有一个碱基差异,显示种内序列高度保守,而与堆型艾美耳球虫、鼠肉孢子虫、刚地弓形虫相应的序列存在不同程度的差异。结果表明,28S rRNA基因部分序列可作为研究艾美耳球虫种间及其他顶复门原虫遗传变异的标记。  相似文献   

3-羰基-桦木酰-甘氨酸的合成与活性分析   总被引：1，自引：0，他引：1  

张小丽  吕坤  方桂珍  田水清  孔维 《北京林业大学学报》2011,33(1):134-138

为了增强桦木酮酸的生物活性,以桦木酮酸为原料,草酞氯为酰化剂,合成桦木酮酸酰氯,再与甘氨酸甲酯盐酸盐合成3-羰基-桦木酰-甘氨酸甲酯,水解得到3-羰基-桦木酰-甘氨酸.采用红外、核磁和质谱对产物进行化学结构分析,并用四唑盐比色试验(MTT)法对人源肺腺癌细胞(A549)、人源鼻癌细胞(CNE)、人源舌鳞癌细胞(Tca8...  相似文献   

BPS28发酵液抗菌谱及抗菌物质稳定性测试   总被引：1，自引：1，他引：0  

姜海波  徐文静  杜茜  许修宏  李启云 《吉林农业科学》2009,34(1)

链霉菌菌株Streptomyces baichengensis BPS28是本实验室分离得到的一株对多种植物病原菌有拮抗作用的菌株。本文对BPS28发酵液的抗菌谱及在不同条件下的稳定性进行了测定,结果表明,BPS28对实验中的21种病原菌均有很好的抑菌作用,对水稻稻瘟病的抑菌率达75%以上,其发酵液的热稳定性差、光稳定性较好、在pH3～7的环境下稳定、不宜室温保存、可在4℃冰箱保存1～6个月。  相似文献   

DH33-28速生桉优良无性系丰产培育技术     

廖立军 《安徽农学通报》2009,15(13):49-50

DH33-28是广西国营东门林场采用尾叶桉×巨桉F1代再通过无性繁殖选育成的杂交桉无性系,该无性系具有干形通直圆满,长势旺盛、木材产量高等特点。介绍了其高产栽培技术。  相似文献   

Potential role of LvDscam in specific immune response of Litopenaeus vannamei against white spot syndrome virus by oral delivery of VP28 using Bacillus subtilis          下载免费PDF全文

Ling‐Lin Fu  Tao‐Ping Li  Yanbo Wang 《Aquaculture Research》2016,47(7):2068-2079

Envelope protein VP28 has been suggested as a candidate vaccine component to evoke a better protection against white spot syndrome virus (WSSV). We have reported that Bacillus subtilis spores harbouring VP28 (rVP28‐bs) can specifically protect shrimp against WSSV. However, the mechanism that supports the production of unique molecules induced by rVP28‐bs to trigger specific immunity is originally unknown. It has recently been suggested that Dscam (Down syndrome cell adhesion molecule) plays an essential role in the alternative adaptive immunity of invertebrates. In this study, we compared the diversity of Litopenaeus vannamei Dscam (LvDscam) variable regions by different antigens immunization. A total of 13, 15 and 11 expressed alternative sequences were identified for N‐terminal Ig2, N‐terminal Ig3 and the entire Ig7 domain, respectively. More than half of the unique variants (16 out of 22) were found in the Ig2/Ig3 domains. Further analysis of the interaction between VP28 and unique Ig2/Ig3 variants was confirmed by both yeast two‐hybrid and GST pull‐down approach. We also found that the percentage of haemocytes phagocytosing WSSV was significantly higher (P < 0.001) in the shrimp injected with control‐siRNA (43.8 ± 2.2) than those with Dscam‐siRNA (11.3 ± 5.4) in the rVP28‐bs groups. With Dscam‐siRNA injection, survivorship significantly decreased (P < 0.001) in the rVP28‐bs group after WSSV challenge. Our data suggested that LvDscam‐mediated pathway may be involved in the specific immune response of shrimp against WSSV induced by rVP28‐bs.  相似文献   

淮稻5号的真菌多样性及其储藏过程中可培养的优势真菌   总被引：2，自引：0，他引：2  

都立辉  和肖营  刘凌平  袁建  鞠兴荣 《中国农业科学》2016,49(7):1371-1381

【目的】从传统形态学和分子生物学两方面对2013年的淮稻5号中分离的真菌进行鉴定,并研究淮稻5号在不同储藏条件下优势菌株的演变规律,为该品种稻谷储藏过程中的真菌种属研究及霉变防控提供参考。【方法】淮稻5号中的可培养真菌通过采用孟加拉红和马铃薯葡萄糖琼脂（PDA）两种培养基,按照国标GB 4789.15-2010的操作获得。将采自4个不同地区的淮稻5号混匀,分别调节其水分含量为14.5%和18.5%后放入25℃人工气候箱内模拟储藏,逐月取出模拟储藏的稻谷样品进行传统培养以确定可培养的优势真菌。真菌的种属分别通过菌落、菌丝及孢子的形态学观察,对照《中国真菌志》等参考文献确定;同时通过PCR分别扩增相应真菌的内转录间隔区（ITS）和28S rDNA的部分序列,连接T载体后测序,通过构建系统发育树进而确定真菌的种属。【结果】从4个地区的2013年淮稻5号稻谷中共分离得到33株真菌,主要为青霉（Penicillium）、曲霉（Aspergillus）和镰刀菌（Fusarium）等菌属,其中青霉属与曲霉属最多,分别为16株和15株,分别占分离所得菌株的48.5%和45.5%,表明青霉和曲霉是2013年淮稻5号稻谷中可培养真菌的优势菌属。而镰刀菌属的真菌仅2株,仅占分离所得菌株的6%。模拟储藏的结果表明,水分含量为14.5%的淮稻5号在25℃条件下储藏6个月时出现优势菌株,为橘灰青霉（Penicillium aurantiogriseum）,并且该优势菌在模拟储藏的第7、8个月持续存在。水分含量为18.5%的淮稻5号在25℃条件下储藏7个月时出现优势菌株,为酵母菌,且该优势菌在模拟储藏8个月时依然存在。【结论】2013年淮稻5号中分离获得33株真菌,主要包括16株青霉、15株曲霉以及2株镰刀菌,表明青霉和曲霉是2013年淮稻5号稻谷中的优势菌属。不同水分含量的稻谷在储藏过程中可能出现不同的优势菌株。  相似文献   

基因工程菌发酵秸秆水解液产生物柴油     

王兵  竺利红  赵宇华  王欣  孙东昌 《浙江农业学报》2014,26(2):0

目前生物柴油的价格是石化柴油的15倍,寻求新的廉价易得的原料降低生物柴油的成本是必然趋势。作物秸秆是一种低廉普遍的废弃物,秸秆中含有纤维素,半纤维素等成分,水解成单糖后可以作为合成生物柴油所需的糖类来源。利用前期研究构建的基因工程菌Escherichia coli pET28a(+) PAW发酵秸秆(玉米秸秆和小麦秸秆)水解液得到乙醇,并在胞内将乙醇与外源添加的脂肪酸进行同步转化合成生物柴油的主要成分脂肪酸乙酯。HPLC分析表明玉米水解液中葡萄糖含量(1040 g·L-1)较高,小麦水解液中木糖含量(4056 g·L-1)较高。E. coli pET28a(+) PAW能够有效地以小麦和玉米两种秸秆水解液作为糖类替代物发酵生产生物柴油,小麦水解液培养基中的生物柴油含量(030 g·L-1)高于玉米水解液培养基中的含量(025 g·L-1),同时也高于现有报道。结果表明,以秸秆水解液作为原料,利用基因工程菌合成生物柴油是可行的,有助于降低生物柴油的原料成本。  相似文献