6-二甲基氨基嘌呤诱导泥蚶三倍体实验   总被引：1，自引：0，他引：1  

吕振明  李太武  柴雪良  方军 《河北渔业》2002,(1):15-15,20

于2001年5月,用6-DMAP抑制泥蚶受精卵第二极体的释放诱导三倍体的产生。实验设三个6-DMAP浓度梯度,分别为300μmol/L、450μmol/L、600μmol/L,处理时机为受精卵第一极排放体30％,处理持续时间为10min。结果:三倍体诱导率300μmol/L时为35.5％,450μmol/L时为48.6％,600μmol/L时约为58.5％,另外本实验还就6-DMAP浓度对D形幼虫孵化率和畸形率的影响以及在染色体制片过程中的有关问题进行了初步探讨。  相似文献   

Kojic acid inhibited melanin synthesis by tyrosinase pathway in Pteria penguin     

Feifei Yu  Xiaoyu Tang  Bingliang Qu  Zhiming Zhong  Wanyu Zhang  Xiangyong Yu 《Aquaculture Research》2020,51(4):1584-1591

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大头鳕TNFSF6基因的结构分析及其在发育早期和病毒暴发时的表达水平     

刘瑞婷  蒋洁兰  毛明光  姜志强  布尔特  矫志伟  陈明康  孙谦 《水产学报》2019,43(2):523-531

为揭示大头鳕TNFSF6基因的基本结构与功能及其在大头鳕发育和神经坏死病毒(Pacific cod nervous necrosis virus, PCNNV)暴发时的响应机制,本研究通过基因克隆获得TNFSF6 cDNA开放阅读框序列,并对序列进行生物信息学分析,运用相对荧光定量PCR(qRT-PCR)方法对TNFSF6在不同组织、孵化后不同日龄仔鱼和PCNNV感染前后仔稚鱼中的表达水平进行检测。结果显示,大头鳕TNFSF6 cDNA长1 388 bp,5′UTR占315bp,3′UTR占500 bp,ORF全长573 bp,编码190个氨基酸。qRT-PCR结果显示,TNFSF6在各组织均有表达,但在脾脏和鳃组织中的表达量较高;大头鳕孵化后15、20、37和40 d TNFSF6基因的转录水平分别是其在5 d转录水平的0.28、0.15、0.12和0.13倍。在24 d和46 d PCNNV暴发时,病鱼TNFSF6基因的转录水平高于对照组;在77 d PCNNV暴发时,病鱼TNFSF6转录水平则显著低于对照组。研究表明,TNFSF6基因在大头鳕发育早期和仔鱼暴发PCNNV时发挥了重要的作用。  相似文献   

Effect of dietary vitamin B6 supplementation on growth and intestinal microflora of juvenile golden pompano (Trachinotus ovatus)     

Qianqian Huang  Heizhao Lin  Ruixuan Wang  Zhong Huang  Chuanpeng Zhou  Wei Yu  Pengwei Xun  Lianjie Tan  Yun Wang  Jun Wang 《Aquaculture Research》2019,50(9):2359-2370

In order to investigate the dietary vitamin B₆ (VB₆) requirement for juvenile golden pompano, an experiment of six different diets with six dietary VB₆ levels (0, 3.75, 7.47, 10.6, 13.7 and 18.5 mg/kg) was conducted. The results indicated that the content of dietary VB₆ significantly increased weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR; p < 0.05). Golden pompano fed diet containing 7.47 mg/kg VB₆ achieved the maximum ALT, AST, GPx, hepatic VB₆ concentrations as well as the minimum MDA, however, the ALT, AST, GPx and hepatic VB₆ concentrations decreased while MDA increased with a further increase in dieatry VB₆. The highest values of GR and POD occurred at the 10.6 and 13.7 mg/kg dietary VB₆ (p < 0.05) respectively. A diet supplemented with 7.47 mg/kg VB₆ increased intestinal Na⁺, K⁺‐ATPase, Chymotrypsin, γ‐glutamyl transpeptidase, creatine kinase and amylase activities (p < 0.05). The relative abundance of Tenericutes and Bacteroidetes decreased while Proteobacteria and Firmicutes improved with an increase in dietary VB₆ levels up to 7.47 mg/kg. Quadratic regression analysis on WGR, hepatic VB₆ concentrations, AST and ALT indicated that the optimum dietary VB₆ levels for juvenile golden pompano were 8.84–9.28 mg/kg.  相似文献   

中华鲟ASDIGIRR与ASTRAF6基因的克隆与表达     

张利凤  汤东东  郑楚文  邓丹  郭慧芝  张书环  张文兵  许巧情  李宁求 《水产学报》2021,45(8):1296-1306

中华鲟是处在硬骨鱼类与软骨鱼类之间的中国古老的珍稀鱼类之一,但在人工繁殖和养殖过程中,容易受到不同疾病的危害,因此,需要深入研究其免疫调控,为其疾病预防提供理论依据。单个免疫球蛋白白细胞介素-1受体相关分子(SIGIRR)和肿瘤坏死因子受体相关因子6(TRAF6)是toll样受体(TLR)信号通路的2个重要的信号转导元件。本研究鉴定了中华鲟的SIGIRR和TRAF6的同源物,分别命名为ASDIGIRR (Acipenser sinensis DIGIRR)和ASTRAF6 (A. sinensis TRAF6),并研究它们在正常组织中的表达和Poly(I:C)诱导后的表达模式。结果显示,ASDIGIRR和ASTRAF6在健康鲟的10个组织中均有表达,且分别在肠道和头肾组织中表达量最高;用Poly (I:C)孵育中华鲟脾脏细胞后,ASDIGIRR在3 h时的表达量显著下调,在6 h时恢复至正常水平,后在24 h显著上调达到最高值,随后开始下调,至48 h时仍显著高于对照组,而ASTRAF6在6 h达到最高表达量,维持到24 h后再下调,48 h时下降至最低水平。研究表明,ASDIGIRR和ASTRAF6在中华鲟抵御病原入侵的免疫防御过程中起着重要作用。  相似文献   

Effect of crude oil extracts from trout offal as a replacement for fish oil in the diets of the Australian native fish Murray cod Maccullochella peelii peelii     

Giovanni M Turchini  Rasanthi M Gunasekera  & Sena S De Silva 《Aquaculture Research》2003,34(9):697-708

The efficacy of trout oil (TO), extracted from trout offal from the aquaculture industry, was evaluated in juvenile Murray cod Maccullochella peelii peelii (25.4±0.81 g) diets in an experiment conducted over 60 days at 23.7±0.8 °C. Five isonitrogenous (48% protein), isolipidic (16%) and isoenergetic (21.8 kJ g^?1) diets, in which the fish oil fraction was replaced in increments of 25% (0–100%), were used. The best growth and feed efficiency was observed in fish fed diets containing 50–75% TO. The relationship of specific growth rate (SGR), food conversion ratio (FCR) and protein efficiency ratio (PER) to the amount of TO in the diets was described in each case by second‐order polynomial equations (P<0.05), which were: SGR=–0.44TO²+0.52TO+1.23 (r²=0.90, P<0.05); FCR=0.53TO²–0.64TO+1.21 (r²=0.95, P<0.05); and PER=–0.73TO²+0.90TO+1.54 (r²=0.90, P<0.05). Significant differences in carcass and muscle proximate compositions were noted among the different dietary treatments. Less lipid was found in muscle than in carcass. The fatty acids found in highest amounts in Murray cod, irrespective of the dietary treatment, were palmitic acid (16:0), oleic acid (18:1n‐9), linoleic acid (18:2n‐6) and eicosapentaenoic acid (20:5n‐3). The fatty acid composition of the muscle reflected that of the diets. Both the n‐6 fatty acid content and the n‐3 to n‐6 ratio were significantly (P<0.05) related to growth parameters, the relationships being as follows. Percentage of n‐6 in diet (X) to SGR and FCR: SGR=–0.12X²+3.96X–32.51 (r²=0.96) and FCR=0.13X²–4.47X+39.39 (r²=0.98); and n‐3:n‐6 ratio (Z) to SGR, FCR, PER: SGR=–2.02Z²+5.01Z–1.74 (r²=0.88), FCR=2.31Z²–5.70Z+4.54 (r²=0.93) and PER=–3.12Z²–7.56Z+2.80 (r²=0.88) respectively. It is evident from this study that TO could be used effectively in Murray cod diets, and that an n‐3:n‐6 ratio of 1.2 results in the best growth performance in Murray cod.  相似文献   

Regeneration of adventitious shoots from mature stored cotyledons of Japanese plum (Prunus salicina Lind1)     

F.A. Canli  L. Tian 《Scientia Horticulturae》2009

Adventitious shoot regeneration from mature stored cotyledons of Japanese plum (Prunus salicina Lind1) was achieved in vitro. The influences of the presence and absence of the light, different concentrations of thidiazuron (TDZ) and benzyladenine (BA) in the culture media, TDZ pretreatments and different basal salts on shoot regeneration were evaluated. TDZ was more effective in inducing shoot regeneration from mature stored cotyledons than BA. Dark incubation significantly increased the regeneration frequencies. Quoirin/Lepoivre (QL) basal salts stimulated shoot regeneration more than woody plant (WPM) or B5 salts did. The frequency of adventitious shoot formation varied among the varieties and the regeneration ability appeared to be genotype depended. The frequency of regeneration under the optimum tested conditions for ‘Bruce’, ‘Shiro’, ‘Redheart’, ‘Gladstone’ and ‘Early Golden’ cotyledons were 66.7%, 46.7%, 43.3%, 26.7% and 6.7%, respectively.  相似文献   

In vitro high frequency direct plant regeneration from whole leaves of blackberry     

Sandhya Gupta  V. Mahalaxmi 《Scientia Horticulturae》2009

High frequency and direct (without callus) plant regeneration was achieved from whole leaf explants of thornless blackberry (Rubus hybrid) cv. Black Satin (EC No. 381258; PI No. 553272) in vitro. Leaf blade explants from 1-, 3- and 5-month-old mother cultures were cultured on Murashige and Skoog (MS) medium with thidiazuron (TDZ), N⁶-benzylaminopurine (BAP), indol-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA), alone or in combination. Three-month explants cultured on 0.02 mg l⁻¹ TDZ produced a high regeneration frequency (91.7%) and the most shoots/leaf explant (17.3). The shoot primordia developed within 3 weeks from the point of detachment of the petiole from the leaf blade. The age of the explant source significantly affected the shoot regeneration potential of the leaf explants. Leaves excised from 3-month-old in vitro-cultured shoots performed better than those from 1- and 5-month-old shoots. Shoots rooted best on half-strength MS basal medium with 0.5 mg l⁻¹ IBA and 90% of the plantlets survived acclimatization. The regenerated plantlets were morphologically similar to the mother plants.  相似文献   

Floral induction (FI) in longan (Dimocarpus longan, Lour.) trees. III: Effect of shading the trees on potassium chlorate induced FI and resulting hormonal changes in leaves and shoots     

K. Sringarm  P. Potchanasin  D. Naphrom  K.F. Bangerth 《Scientia Horticulturae》2009

Previous experiments demonstrated that treatment of longan trees with potassium chlorate (KClO₃) induces “off season floral induction” (FI) even in the absence of the naturally required cool temperature [Manochai, P., Sruamsiri, P., Wiriya-alongkorn, W., Naphrom, D., Hegele, M., Bangerth, F., 2005. Year around off season flower induction in longan (Dimocarpus longan, Lour.) trees by KClO₃ applications: potentials and problems. Sci. Hortic. 104, 379–390]. Potassium chlorate, however, cannot replace the presence of functional mature leaves and sufficient light intensity. Here we examined in more detail the effect of shade (about 10% of natural sunlight) on KClO₃ affected hormone concentrations/transport of leaves and shoot apical buds (SAB) and their interactions with FI.  相似文献   

茄子新品种新茄6号的选育     

王贵忠  张宏伟  冯世强  王超 《中国蔬菜》2009,1(14):66-67

新茄6号是由从新疆长茄中单株套袋自交选育出的自交系4号长茄和从前苏联长茄品种中单株套袋自交选育出的自交系26号长 茄配组,然后在后代分离群体中通过系统选择育成的长茄新品种。植株生长势强,第1花生长在第5~6片叶腋,果实细长,浅紫红色, 光泽度好,平均单果质量140 g,抗黄萎病能力优于新疆长茄,一般每667 m²产量4 500~5 500 kg,适于新疆早春大棚和露地覆膜早 熟栽培。  相似文献