排序方式: 共有94条查询结果,搜索用时 31 毫秒
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莪术CPD染色和45S rDNA荧光原位杂交核型分析 总被引:1,自引:0,他引:1
为了对莪术[Curcuma zedoaria (Christm.) Roscoe]的染色体进行识别并对该物种基因组的结构及进化进行初步研究,利用改进的火焰干燥法及荧光原位杂交技术,对莪术中期染色体的长度,着丝粒的位置及随体的数目进行分析。PI和DAPI组合(CPD)染色后和相继的45S rDNA探针荧光原位杂交结果显示,莪术具有五对45S rDNA位点,三对位于8,22,31号染色体末端的CPD带区,二对位于4,30号染色体的短臂上。第五号短臂为富含GC对的非45S rDNA位点。该实验建立了莪术的经典核型,为非整倍体,核型公式为2n=62+1=40m+12sm+1m,其核型不对称性为2A型。 相似文献
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为了完善污泥两相厌氧消化工艺、优化运行设计参数,对西安市污水处理厂污泥进行了中温两相厌氧消化与中温单相厌氧消化对比试验。结果表明,两相厌氧消化产酸相水力停留时间(HRT)为1.25 d,产甲烷相HRT为11.10 d,当污泥投配率8%,有机负荷>2.0 kg/(m3.d)时,化学需氧量(COD)与挥发性固体(VS)的去除率分别为42.7%和33.6%。在相同有机负荷下,两相厌氧消化工艺的VS和COD去除率、产气量、产气速率以及分解单位VS的产气量均高于单相厌氧消化工艺,而出泥VFA含量小于单相厌氧消化工艺。 相似文献
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Apiraya Thepsukhon Somporn Choonluchanon Shikeyuki Tajima Mika Nomura Soraya Ruamrungsri 《Journal of plant nutrition》2013,36(9):1424-1438
Endophytic bacteria carrying out dinitrogen (N2) fixation and indole acetic acid (IAA) synthesis were firstly identified in C. alismatifolia, a globally important flower crop. Their potential as growth promoters to stimulate the rapid growth of host plant was also examined. It will be beneficial to reduce the propagation period of tissue culture plantlets, and also utilize as a biofertilizer for rhizome production in the field. Seven endophytic bacteria were isolated from the leaf, four isolates from the leaf base, and two from the rhizome. ECS203, a gram-negative bacterium with a round shape, showed the highest N2 fixation at 4.2 nmol C2H4/106 cells/hr, and ECS202 showed the highest IAA synthesis at 296 μL μg ? 1 protein. Three selected isolates of N2-fixing and IAA synthesizing endophytic bacteria, i.e., ECS202, ECS203, and ECS204, isolated from the leaf base, were used to reinoculate Curcuma plantlets derived from tissue culture. Then, plants were grown in sterilized sand for 2 months and weekly supplied with N-free nutrient solution. Plant growth, colonization, nitrogen fixation, and IAA synthesis were measured at two months after planting. The inoculated plants clearly showed a better performance of plant growth and yield in terms of the plant height, plant weight, leaf area, and diameter of new rhizomes compared with uninoculated plants. The chlorophyll content and N concentration of leaves and roots also increased in inoculated plants. Endophytic bacteria from inoculated plants colonized the roots, rhizome, and leaf base. Partial sequence analysis using 16S rDNA indicated that the isolate ECS202 corresponded to Sphingomonas pseudosanguinis (99.2% similarity over 1,371 bp), ECS203 to Bacillus drentensis (99.4% similarity over 1,450 bp) and ECS204 to Bacillus methylotrophicus (99.9% similarity over 13,06 bp). 相似文献
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桂郁金种质资源姜黄素类化学成分分析 总被引:1,自引:0,他引:1
[目的 ]探索桂郁金种质资源姜黄素类化学成分分析方法。 [方法 ]:HPLC法被用以检测桂郁金姜黄素类成分,选用Agilent SB-C18色谱柱(250mm×4.6mm,5μm),流动相为乙腈-冰醋酸水溶液,采用梯度洗脱,以420 nm作为检测波长,以30℃作为柱温;流速1 mL·min-1。 [结果 ]桂郁金种质资源姜黄素类化学成分在测定范围内线性关系良好;符合要求的试验包括精密度、稳定性、重复性、加样回收试验。桂郁金姜黄素类化学成分的种类和含量由于种质不同而存在明显差异。 [结论 ]该方法操作简单,适用于桂郁金种资源姜黄素类化学成分分析, 其中桂郁金种质B71、C16在姜黄素类成分含量方面表现突出,为桂郁金优良种质。 相似文献
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姜荷花切花保鲜技术研究 总被引:1,自引:0,他引:1
利用正交设计法探讨了漂白粉、硫酸铝、蔗糖8、-羟基喹啉(8-HQ)、柠檬酸5种不同因素对姜荷花切花保鲜效果的影响,结果表明:蔗糖是影响姜荷花切花寿命的主要因素,其次是8-HQ、硫酸铝、漂白粉、柠檬酸,而延长姜荷花切花瓶插寿命的最优保鲜剂配方为50 mg/L漂白粉+300 mg/L硫酸铝+2%蔗糖+100 mg/L 8-HQ。 相似文献
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为改变姜荷花主要切花品种清迈粉存在的花茎细长、易倒伏等缺点,使之适合盆栽化需求,并对其矮化过程中的组织结构进行解剖分析。以烯效唑处理姜荷花植株后,株高、花茎长明显变短,而茎粗以及花茎粗仅有轻微的增加,效果并不显著;此外,150 mg·L-1烯效唑处理对分蘖数有明显的增加作用。对其进行组织解剖分析可知,花茎的变短变粗与花茎细胞纵向长度变短、横向长度变大有关,对叶片表皮细胞分析可知,烯效唑处理可以使得姜荷花叶片的上下表皮细胞都变小,而气孔数增多,同时上表皮的气孔变小,而下表皮气孔变大。 相似文献