Effects of milk thistle meal on performance,ileal bacterial enumeration,jejunal morphology and blood lipid peroxidation in laying hens fed diets with different levels of metabolizable energy          下载免费PDF全文

N. S. Hashemi Jabali  A. H. Mahdavi  S. Ansari Mahyari  M. Sedghi  R. Akbari Moghaddam Kakhki 《Journal of animal physiology and animal nutrition》2018,102(2):410-420

This study was conducted to evaluate the effects of different levels of milk thistle meal on performance, blood biochemical indices, ileal bacterial counts and intestinal histology in laying hens fed diets containing different levels of metabolizable energy. A total number of 200 Leghorn laying hens (Hy‐Line W‐36) were randomly assigned to eight experimental treatments with five cage replicates of five birds each. Dietary treatments consisted of four levels of milk thistle meal (0%, 15%, 30% and 60%) and two levels of AME_n (11.09 and 12.34 MJ/kg) fed over a period of 80 days. In vitro studies revealed that the total phenolic component of milk thistle meal was 470.64 mg gallic acid equivalent/g of the sample, and its antioxidant activity for inhibiting the 2‐2‐diphenyl‐1‐picrichydrazyl free radical and reducing ferric ions was about 21% higher than that of butylated hydroxyltoluene (p < .05). Diets containing high level of AME_n led to improved egg production (p < .05), egg weight (p < .05), egg mass (p < .01) and feed conversion ratio (p < .01). In addition, offering diets containing high energy significantly enhanced (p < .01) serum triglyceride and malondialdehyde (MDA) concentrations as well as jejunal villus height. Dietary supplementation of 3% milk thistle meal resulted in the best feed conversion ratio (p < .05), reduction of ileal Escherichia coli enumeration (p < .01) and an enhancement in the villus height‐to‐crypt depth ratio (p < .05). Furthermore, feeding incremental levels of this meal led to remarkable decrease in serum cholesterol, triglyceride and MDA (p < .01) concentrations while significant increase in blood high‐density lipoprotein content and goblet cell numbers (p < .05). The present findings indicate that milk thistle meal with high antioxidant and antibacterial properties in laying hen diets may improve health indices and productive performance.  相似文献   

Polyphenols in Eucalyptus leaves improved the egg and meat qualities and protected against ethanol‐induced oxidative damage in laying hens          下载免费PDF全文

W. Li  J. Miao  N. Chen  X. Shao  Y. Cao 《Journal of animal physiology and animal nutrition》2018,102(1):214-223

Polyphenols in Eucalyptus leaves (PE) were value resources due to various pharmacological activities, but data on the effect on laying hens are very scare. This study was conducted to determine the effect of PE on the laying performance, egg traits, meat quality, antioxidant status and liver tissues of laying hens. One hundred and twenty 256‐day‐old Yueqinhuang laying hens were randomly assigned to four treatment groups (different levels of PE at 0, 0.5, 0.8 and 1.2 g/kg diet) for 63 days with 15 replicates per group. Addition of 0.8 g/kg PE not only improved the egg trait by increasing the eggshell thickness and decreasing MDA production and cholesterol content in the egg yolk, but also significantly enhanced meat quality of hens, as suggested by the increase pH_45 min (p < .01) and a* value, and decrease drip loss rate (p < .01). Meanwhile, under acute ethanol‐induced oxidative damage condition, supplementation of 0.8 g/kg PE enhanced the serum antioxidant status by increasing enzymatic activities (GSH‐PX, T‐SOD, T‐AOC), inhibited oxidative damage and provided protective effect to liver tissue. In conclusion, addition of 0.8 g/kg PE not only improved the egg traits and meat quality without obvious adverse effects, but also increased the serum antioxidant status of the hens and protected their liver tissue from ethanol‐induced oxidative damage. This study indicated that PE could be utilized as an effective feed additive for laying hens to improve health performance and egg traits.  相似文献   

Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats          下载免费PDF全文

M Narasimhaiah  A Arunachalam  S Sellappan  VK Mayasula  PR Guvvala  SK Ghosh  V Chandra  J Ghosh  H Kumar 《Reproduction in domestic animals》2018,53(3):644-654

Trace minerals feeding had significant effects on sperm production and fertility with better absorption and proper utilization within the body for optimum reproductive function. Several studies have shown that more influenced trace elements in the diets of animals are copper (Cu) and zinc (Zn). Bucks showing deficiency of this mineral might affect the quality of semen production which in turn would affect the fertility. This experiment was thus designed to test the effects of organic Cu and Zn supplementation on antioxidants enzyme activities and sperm functional attributes in fresh semen of bucks. Forty bucks (n = 40, Aged 5 months) were assigned to ten groups of four animals in each group, supplemented (for a period of 8 months) with different levels of organic Zn: 20 mg (T2), 40 mg (T3) and 60 mg (T4), organic Cu: 12.5 mg (T5), 25 mg (T6), 37.5 mg (T7) and combined organic Zn and Cu: 20 + 12.5 mg (T8), 40 + 25 mg (T9), 60 + 37.5 mg (T10), respectively, per kg dry matter and no additional mineral diet (control; T1). One hundred and sixty semen samples were collected through electro‐ejaculator and analysed for sperm quantity, quality, acrosome intactness and plasma membrane integrity and correlated with the catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase enzyme activities in seminal plasma. The results indicated organic Cu and zinc supplemented bucks produced more sperm cells, had higher sperm concentrations, maintained higher (p < .01) sperm livability, plasma membrane and acrosome integrities, more motility and velocity. The increased antioxidant enzyme activities, reduced oxidative stress and lowered lipid peroxidation were positively correlated (p < .05) with the sperm functional attributes. In conclusion, organic Cu and Zn supplement to male goats showed protective roles against oxidative damage and maintained better fresh semen characteristics.  相似文献   

黑茶茶多酚类物质的提取及其抗氧化性能研究     

陈红艳  李雨浩  岑浩彬 《中国农业大学学报》2017,22(9):101-107

以湖南安化天尖、茯砖及广西六堡3种黑茶为考察对象,研究黑茶中茶多酚的提取工艺以及抗氧化性能。针对超声波不同提取条件,采用正交试验,确定黑茶中茶多酚的最佳提取工艺条件;采用清除二苯代苦味酰自由基(DPPH)的能力和邻苯三酚自氧化体系来评价其抗氧化活性。结果表明:采用超声波热水复合浸提法提取黑茶中茶多酚的最佳提取条件为超声波提取功率250 W,提取固液比1∶25,提取温度55℃,提取时间45min,茯砖黑茶中茶多酚的提取率5.21%;同时测得茯砖黑茶具有较好的抗氧化能力,对DPPH自由基有较强的清除能力,但与维生素C(VC)相比,其抗氧化能力比VC的能力弱;其清除超氧阴离子自由基能力的IC50为0.507μg/mL。  相似文献   

Copper‐Induced Oxidative Stress and Responses of the Antioxidant System in Roots of Medicago sativa     

S.‐H. Wang  H. Zhang  Q. Zhang  G.‐M. Jin  S.‐J. Jiang  D. Jiang  Q.‐Y. He  Z.‐P. Li 《Journal of Agronomy and Crop Science》2011,197(6):418-429

The effects of various copper (Cu) concentrations on the antioxidative system in the roots of Medicago sativa were explored. The results indicated that the Cu content of the roots reached a value of 854 μg g^?1 DW at 10 μm Cu and a value of 4415 μg g^?1 DW at 100 μm Cu, suggesting that M. sativa has better ability to tolerate and accumulate Cu than other Cu‐bioaccumulators, and is a potential plant for phytoremediation. Treatment with Cu resulted in a significant increment in the levels of H₂O₂, O₂˙^? and OH˙. The reduced form of ascorbate and glutathione reached a peak at 30 μm Cu, and was followed by a sharp depletion to a lower level than that of the control. In contrast, the levels of the oxidised forms of ascorbate and glutathione showed a progressive increment with increasing Cu concentrations, suggesting that the antioxidant system was unable to cope with Cu stress at higher Cu levels. Under the Cu concentrations tested, the activity of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) increased at lower Cu concentrations, and then decreased, reaching a maximum at 30 μm of Cu for APX and GR, at 10 μm for CAT, whereas the activities of guaiacol peroxidase (POD, EC 1.11.1.7) were gradually increased with increasing Cu concentrations. PAGE analysis of superoxide dismutase (SOD, EC 1.1.5.1.1) revealed that one band is a Mn‐SOD and five bands are identified as Cu, Zn‐SOD, whereas Fe‐SOD isoforms were not found in the roots of alfalfa. Cu at 10–100 μm increased the intensity of constitutive isozymes of CAT, APX and POD, whereas it decreased the intensity of isozymes of glucose‐6‐phosphate dehydrogenase (G6PDH, EC 1.1.1.49) significantly. The activities of lipoxygenases (LOX, EC 1.13.11.12) were gradually augmented with increasing Cu concentrations, demonstrating that LOXs are probably involved in production of lipid hydroperoxides and superoxide anion. There was a continuous and pronounced enhancement in the activity of esterase (EST, EC 3.1.1.1) in roots treated with 10–30 Cu μm , whereas EST activity in roots exposed to above 30 μm Cu declined, suggesting that EST plays a protective role under lower Cu concentrations stress.  相似文献   

生长调节物质对小豆豆芽营养成分影响和功能性评价     

郑琳琳  常暖迎  庞肖杰  康玉凡 《中国农业大学学报》2019,24(8):19-26

为筛选最适合芽菜培养的小豆品种,以‘白红8号’为试材,设置3种生长调节物质(乙酰水杨酸、乙烯利和6-苄氨基腺嘌呤(6-BA))的各6个浓度(0.0、0.1、0.2、0.3、0.4、0.5 mmol/L;0、20、30、40、50、60 mg/L和0、2、3、4、5、6 mg/L)喷施处理,测定其生物特性(上胚轴长度、上胚轴直径、萌发率、鲜重、干重和产出比)、营养成分(可溶性蛋白和可溶性糖)及抗氧化等特性。结果表明:3种生长调节物质喷施处理都能够提升其营养价值,在乙烯利喷施浓度为50 mg/L,‘白红8号’小豆芽苗菜中各营养成分、酶活性及抗氧化性等均可达到最大值,显著提高了其营养价值。因此,为提高生产中小豆芽苗菜的产量和营养价值,最终确定乙烯利喷施浓度为50 mg/L效果最佳。  相似文献   

煮熟和干燥处理对黄藤笋抗氧化活性的影响     

黄世能  黄雪梅  牛国才  张昭其 《世界竹藤通讯》2014,(3):1-4

通过测定黄藤熟笋、生笋(鲜切笋)、熟笋干和生笋干的水和4种不同浓度乙醇提取物中的自由基清除能力、总还原能力、总多酚含量和总黄酮含量,研究了煮熟、干燥加工方法对黄藤笋提取物抗氧化活性的影响。结果表明,煮熟处理能保持或提高提取物的抗氧化活性,干燥处理则相反。乙醇提取物比水提取物具有更高的抗氧化活性,以50%~75%的乙醇提取溶剂效果最好。熟笋在所有溶剂中的自由基清除能力、总还原能力、总多酚含量和总黄酮含量均表现出最高水平,表明煮熟是黄藤笋适宜的加工利用方式。  相似文献   

长蒴黄麻总黄酮的提取及其抗氧化性   总被引：1，自引：0，他引：1  

陈如冰  周雪娜  陈婉芳  郑加玲  林燕如 《贵州农业科学》2011,(10):162-165

为长蒴黄麻的开发利用提供参考依据,对超声波协同乙醇提取长蒴黄麻黄酮的效果进行了研究,并进行了长蒴黄麻黄酮的抗氧化性试验.结果表明,最优提取条件为温度42℃、料液比1∶35、乙醇浓度80％、提取时间25min,在此条件下长蒴黄麻总黄酮的提取率为2.577％.长蒴黄麻黄酮提取物对羟基自由基和超氧阴离子自由基具有一定的清除作...  相似文献   

Effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and antioxidant capacity     

Yang Zi  Chi Ma  Huimin Li  Suting Shen  Yingchun Liu  Ming Li  Feng Gao 《Animal Science Journal》2021,92(1):e13613

This study investigated the effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and renal antioxidant capacity. Eighteen ewes pregnant were randomly divided into control group (CG, ad libitum, 0.67 MJ ME·BW^−0.75·day⁻¹, n = 6), restricted group 1 (RG1, 0.18 MJ ME·BW^−0.75·day⁻¹, n = 6), and restricted group 2 (RG2, 0.33 MJ ME·BW^−0.75·day⁻¹, n = 6). At 140 days, the fetal blood, allantoic fluid and kidney tissue were collected to determinate fetal renal function and renal antioxidant capacity. The results showed that the fetal weight, kidney weight, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), aquaporin-2 (AQP-2) and aquaporin-3 (AQP-3), and total antioxidant capacity (T-AOC) in RG1 group were decreased compared with the CG (P < 0.05), but the contents of β2-Microglobulin (β 2-MG), cystatin C (Cys-C), filtered sodium excretion fraction (FENa), malondialdehyde (MDA), and hydroxyl radical (OH) in RG1 group were increased (P < 0.05). The impaired ovine fetal renal growth, antioxidant imbalance and dysfunction of glomerulus ultrafiltration, and the renal tubules reabsorption were induced by maternal malnutrition during late pregnancy.  相似文献   

Comparison of phenolic profiles and antioxidant activities in skins and pulps of eleven grape cultivars(Vitis vinifera L.)     

Fu-xiang LI  Fu-hua LI  Ya-xuan YANG  Ran YIN  Jian MING 《农业科学学报》2019,18(5):1148-1158

Eleven grape cultivars were analysed to explore the variety differences of fresh grape phenolic profiles. The results showed that free phenolics were predominant in grape skins and pulps, and showed the higher antioxidant activities than bound. In 11 cultivars, Muscat Kyoho extracts had the highest total phenolic content in skins(10.525 mg GAE g~(–1) FW) and pulps(1.134 mg GAE g~(–1) FW), and exhibited the highest DPPH radical scavening capacity(EC_(50)=11.7 μg mL~(–1)) and oxygen radical absorbance capacity(ORAC) value(190.57 μmol TE g~(–1) FW) of free phenolic in skin. In addition, the most abundant phenolics in grape skins were found to be flavonoids such as kaempferol in Kyoho skin(541.2 μg g~(–1) FW), rutin, catechin and epicatechin in Muscat Kyoho skin(262.3, 86.3 and 70.0 μg g~(–1) FW, respectively). Furthermore, the principal component analysis showed a strong difference of phenolic profiles with the cultivars, existing forms and distributions. Pearson correlation coefficient analysis showed a significant linear correlation between total phenolic content and antioxidant activity(P0.05). Therefore, both skins and pulps were rich sources of bioactive phenolic compounds, and Muscat Kyoho was the ideal source among all samples.  相似文献