Cloning, nucleotide sequence and phylogenetic analyses, and tissue-specific expression of the transferrin gene in Cirrhinus mrigala infected with Aeromonas hydrophila     

Sahoo PK  Mohanty BR  Kumari J  Barat A  Sarangi N 《Comparative immunology, microbiology and infectious diseases》2009,32(6):527-537

Transferrin partial complementary DNAs were cloned from the livers of five species in four genera of Indian carps (Indian major carp species: Labeo rohita, Catla catla and Cirrhinus mrigala; medium carp: Puntius sarana; minor carp: Labeo bata) subsequent to polymerase chain reaction amplification with published heterologous primers or self-designed primers derived from conserved regions of transferrin cDNA sequences. The partial transferrin cDNAs of the five species of carps had sizes from 624 to 633 bp (487 bp for L. rohita) and encoded an open reading frame consisting of 206–211 (162 for L. rohita) amino acids. The alignments of carp cDNA sequences showed 85–97% homology and 71–93% homology in deduced amino acid sequences. A phylogenetic tree of amino acid sequences of transferrin cDNAs from carps showed that the relationship among the four genera of Indian carps is well correlated with that derived from classic morphologic analyses. The hypothesized cleavage site and interdomain bridge of transferrin molecule were predicted for the above carp species and interestingly the cleavage site amino acid sequence was found to be conserved among all the carps. To study the tissue-specific expression of the transferrin gene, various tissues (liver, kidney, spleen, brain, muscle, testis, heart, intestine, gill and fin) from apparently healthy (control), moribund and survived C. mrigala experimentally infected with Aeromonas hydrophila infection were analyzed. Transferrin mRNA was detected only in liver RNA and to lesser extent in brain tissue out of the 10 tissues analyzed irrespective of bacterial infection.  相似文献   

Extrapituitary and Pituitary Pathological Findings in Horses with Pituitary Pars Intermedia Dysfunction: A Retrospective Study     

Christiana M. Glover  Lisa M. Miller  Noel O. Dybdal  Alfonso Lopez  Wendy M. Duckett  Dianne McFarlane 《Journal of Equine Veterinary Science》2009

The objective of this study was to describe the histopathologic changes observed in extrapituitary organs as well as the pituitary glands of horses with pituitary pars intermedia dysfunction (PPID). Adrenal gland, thyroid gland, liver, lung, kidney, heart, and pituitary gland from 32 horses with clinical and histologic evidence of PPID and 20 control horses were reviewed histologically. Ten of the control horses were aged animals (≥15 years), allowing those changes attributed to age to be identified. In addition to previously reported changes in adrenal gland and liver, an association was established between PPID and several extrapituitary histopathologic changes, namely bronchiolitis, proliferative glomerulopathy, and myocardial lipofuscinosis and fibrosis. The potential biologic significance of these changes is discussed and, although the retrospective design of the current study precludes establishment of causal relationships between the observed extrapituitary changes and PPID, these findings suggest that further investigations are warranted.  相似文献   

Correlation of Plasma Insulin Concentration with Laminitis Score in a Field Study of Equine Cushing's Disease and Equine Metabolic Syndrome     

Donald M. Walsh  Catherine M. McGowan  Thomas McGowan  Stephen V. Lamb  Barbara J. Schanbacher  Ned J. Place 《Journal of Equine Veterinary Science》2009

This study aimed to investigate endocrinologic test values and the response to treatment of two commonly encountered causes of endocrinopathic laminitis, equine Cushing's disease (ECD) and equine metabolic syndrome (EMS), in a veterinary practice setting. In particular, the study aimed to determine whether insulin concentration correlated to the severity of clinical laminitis in horses with EMS or ECD. Twenty-five horses were included in the study and assigned to one of three groups: ECD (n = 6), EMS (n = 10), and controls (n = 9). Blood samples were collected at an initial visit and then at regular intervals for the next 12 months. Plasma concentrations of adrenocorticotropin (ACTH), cortisol, and insulin and serum concentrations of glucose and total thyroxine (T4) were obtained. Horses with ECD had significantly higher plasma ACTH concentrations than EMS horses or controls. Horses with EMS and ECD both had significantly higher plasma insulin concentrations than control horses, which was correlated with the Obel grade of laminitis (r = 0.63). After treatment, there was a trend for a reduction in plasma ACTH concentration in horses with ECD. A program of diet and exercise for horses with EMS resulted in reductions in both plasma insulin concentrations and bodyweight, which was variable, depending on the individual. There was a significant correlation between the change in plasma insulin concentration and Obel grade of laminitis (r = 0.69). This study has highlighted the importance of baseline plasma insulin concentration as a potential indicator of the susceptibility of horses to laminitis and the response to a program of diet and exercise.  相似文献   

甘肃黄花蒿超临界CO2萃取产物化学成分的差异性分析   总被引：2，自引：1，他引：1  

姚健  赵保堂  王俊龙  王小芳  吴依茜  张继 《草业科学》2009,26(3):57-63

采用超临界CO2萃取技术联合气相色谱 质谱法检测了黄花蒿Artemisia annua萃取产物化学成分,并与水蒸气蒸馏法相比较。结果从超临界CO2萃取提取物成分中确定了86种化合物,主要成分为8,9 脱氢 9 甲酰环异长叶烯(18.22%);(all Z) 5,8,11,14,17,二十碳五烯酸甲基酯（8.39%）等。从水蒸气蒸馏提取物成分中确定了62种化合物,主要成分为4 甲氧基 6 (2 丙烯基) 1,3 苯丙二氧杂环戊烯（24.28%）;松油醇（17.47%）等。2种方法的共有成分32种,分别占鉴定积分面积的40.54%和79.49%。与传统水蒸气蒸馏法相比,采用超临界CO2法能更加真实、全面地反映黄花蒿的化学组分。  相似文献   

甘肃茵陈蒿超临界CO2萃取产物化学成分的差异性分析   总被引：1，自引：0，他引：1  

姚健  赵保堂  王俊龙  张继 《草业科学》2009,26(4):37-42

采用超临界CO2萃取联合气相色谱-质谱法检测了茵陈蒿Artemisia capillaris萃取产物化学成分,并与水蒸气蒸馏法相比较。结果从超临界CO2萃取提取物成分中确定了76种化合物,从水蒸气蒸馏提取物成分中确定了48种化合物。其中长马鞭草烯酮含量分别为37.35%和26.68%,2种方法测得的成分有所差异。与传统水蒸气蒸馏法相比,采用超临界CO2法更能真实、全面地反映茵陈蒿的化学组分,实验为进一步研究利用茵陈蒿植物资源提供了科学依据。  相似文献   

简述进口兽药再注册申报程序及资料要求     

郭桂芳 《中国兽药杂志》2009,43(2):59-60

根据农业部第442号公告发布的《进口兽药再注册申报资料项目》,介绍了进口兽药再注册申报程序及资料要求。  相似文献   

马立克氏病毒1.8-kb mRNA对其上游双向启动子活性的影响     

丁家波  李延鹏  杨明  李中明  孙爱军  崔治中 《中国兽医学报》2009,29(11)

构建了针对1.8-kb mRNA基因簇潜在阅读框的RNA干扰质粒pP(1.8-kb-RNAi).将该质粒与包含其上游双向启动子的质粒pP(pp38)-CAT和pP(1.8-kb)-CAT共转染鸡胚成纤维细胞(CEF)和MDV rMd5感染的CEF(rMd5-CEF),48 h后,通过测定转染细胞裂解液中氟霉素乙酰转移酶(CAT)的活性确定1.8-kb mRNA被干扰后对双向启动子活性的影响.结果显示,利用pP(1.8-kb-RNAi)质粒干扰1.8-kb mRNA,可以使其上游双向启动子两个方向的活性均显著下降(P<0.01),其中1.8-kb mRNA方向下跌29.5%,pp38方向下跌25.0%.本研究结果证明了1.8-kb mRNA对其上游双向启动子活性有增强作用.  相似文献   

不同处理对大豆浓缩蛋白（SPC）粗蛋白含量及提取率的影响     

李宝山  王际英  张利民  王世信  黄炳山 《饲料与畜牧》2009,(3):25-27

分别用超声波处理不同粉碎粒度的高温脱脂豆粕,然后采用醇法浸提工艺提取其中的大豆浓缩蛋白（SPC）。结果显示：40目、60目及80目豆粕提取的大豆浓缩蛋白中粗蛋白含量分别为51．85％、55．50％和54．32％（P〈0．05）。提取率分别为97．65％、97．51％和95．80％（P〈0．05）;超声波处理之后提取的大豆浓缩蛋白中粗蛋白含量分别为55．44％、55．11％和58．77％（P〈0．05）,提取率分别为97．50％、96．78％和95．65％（P〈0．05）。不同的粉碎粒度对大豆浓缩蛋白中粗蛋白的含量及提取率有显著影响,粒度越小,粗蛋白含量越高,提取率越低;在相同的粉碎粒度下,超声波处理能提高大豆浓缩蛋白中粗蛋白的含量,但是对其提取率无显著影响。  相似文献   

PRDM16——控制BAT和WAT发育的分子开关     

张艳  王松柏  陈小冬 《动物医学进展》2009,30(9):110-113

棕色脂肪组织通过大量表达解偶联蛋白-1(UCP-1)以加强线粒体呼吸作用的解偶联,使能量以热量形式散失,从而抵御肥胖和相关疾病.研究发现,PRDM16是调节棕色脂肪细胞形成的关键转录因子,是控制棕色和白色脂肪细胞特异基因表达程序的分子开关,其机制是C末端结合蛋白和过氧化物酶体增殖剂活性受体γ辅助激活子1 (PPARγ-coactivator-1α and β,PGC-1α/β) 竞争性结合PRDM16,分别形成PRDM16/CtBPs和PRDM16/PGC-1s转录复合物,前者可激活棕色脂肪特异基因的表达,后者则抑制白色脂肪特异基因的表达.论文通过对PRDM16的结构、功能及其作用机制的介绍,有助于研究者了解棕色脂肪组织发育的调控系统,为肥胖和相关疾病的治疗提供新思路.  相似文献   

黄土区刺槐林地土壤水分剖面的垂直分层   总被引：1，自引：0，他引：1       下载免费PDF全文

张波  张建军  李轶涛  隋旭红  郑芳  芦倩 《中国水土保持科学》2010,8(4):39-44

 为从蒸散耗水角度对黄土区刺槐林地土壤水分剖面进行垂直分层,在山西吉县蔡家川流域刺槐林地布设频域反射仪对0～150cm土层分层连续测定土壤水分,综合利用灰关联分析法和有序聚类法对各土层、不同时间蒸散耗水特性进行研究。结果表明:从蒸散耗水角度,黄土区刺槐林地土壤水分可垂直划分为地表植物蒸散耗水层(0～10cm)、林下灌木蒸散耗水层(10～40 cm)、刺槐蒸散耗水层(40～150cm);灰关联分析法和有序聚类法可以综合应用于土壤水分剖面垂直分层研究。分层方法数学理论基础较严密,取得的分层结果与现有相关研究结果基本一致,因此,有较高可信度,可为黄土区刺槐林地土壤水分研究提供参考。  相似文献