野生二粒小麦α-淀粉酶抑制因子编码基因序列分析     

江斌  王际睿  魏育明  颜泽洪  郑有良 《四川农业大学学报》2006,24(3):247-251

对3份野生二粒小麦(Triticum dicoccoides)材料的抗虫24 kDaα-淀粉酶抑制因子编码基因进行分离克隆,得到72个24 kDaα-淀粉酶抑制因子基因,并进行序列分析。结果发现,α-淀粉酶抑制因子在野生二粒小麦中以多拷贝形式存在,经卡方检验初步推断野生二粒小麦中的α-淀粉酶抑制因子成熟蛋白编码基因序列变异类型在供试材料间无显著差异。序列分析表明,24 kDaα-淀粉酶抑制因子成熟蛋白编码基因序列无论在野生二粒小麦种内还是与小麦属的其他物种之间,都具有很高的一致性,说明该基因在进化过程中十分保守。  相似文献   

甘肃滩羊血清酯酶和α-淀粉酶多态性分析   总被引：3，自引：0，他引：3  

李积友  李秉诚  刘霞  折富有  武广善  宋祖才  田世雄  赵承银 《甘肃农业大学学报》2001,36(4):369-373

运用聚丙烯酰胺凝胶电泳技术(PAGE)对甘肃滩羊产区皋兰、景泰及靖远3293只滩羊的血清酯酶(Es)、α-淀粉酶(α-Amy)基因座多态性进行了检测分析。结果表明:甘肃滩羊Es基因座存在3种基因型Es++ 、 Es+- 、 Es-- ,它们由Es+、Es-两个共显性等位基因控制,其中Es+-基因型在3个群体中均占优势,频率分别为0.620 0(皋兰)、0.666 6(景泰)、0.730 0(靖远);在皋兰群体和景泰群体中,Es-基因占优势,基因频率分别为0.540 0和0.548 4,而在靖远群体中优势基因则为Es+,其基因频率为0.525 0。在α-Amy基因座上没有检测到多态性。  相似文献   

松节油微波加热水合反应制松油醇   总被引：6，自引：0，他引：6  

李艳琳  韦藤幼 《福建林学院学报》2003,23(4):335-337

以HAc为催化剂,在微波加热条件下,对松节油直接水合制松节醇进行研究.结果表明:微波加热较传统加热更能促进反应的进行;在优化反应条件下,直接将松节油于85℃水合反应5h,其转化率达85.91%,选择性为0.453.  相似文献   

银耳α–微管蛋白基因的克隆及序列分析     

董吉元  杨双熙  陈叶叶  刘娟  马爱民 《园艺学报》2011,38(5):921-929

 根据GenBank上已发表的其他真菌α–微管蛋白基因序列中的保守区域设计简并引物,扩增出银耳α–微管蛋白基因的cDNA部分序列。分别利用SEFA-PCR方法和RACE技术,克隆了银耳α–微管蛋白基因的DNA和cDNA全长序列。银耳α–微管蛋白基因DNA全长1 962 bp,含有9个内含子,cDNA全长1 347 bp,编码448个氨基酸。生物信息学分析结果表明：银耳α–微管蛋白基因的cDNA序列与新型隐球酵母有80%相似性,编码的蛋白质属于Tubulin_FtsZ超家族中的α–微管蛋白亚家族,氨基酸序列中存在保守的GTP结合区域GGGTGSG,系统发育树显示银耳与新型隐球酵母距离最近。  相似文献   

在羊驼皮肤黑色素细胞中α-黑素细胞刺激素对诱导一氧化氮合酶的影响     

王海东  董彦君  薛霖莉  赫晓燕  杨磊 《经济动物学报》2011,(3):164-167,173

为研究α-黑素细胞刺激素（α-Melanocytestimulatinghormone,α-MSH）对诱导型一氧化氮合酶（NOS2）在羊驼皮肤黑素细胞中的影响。采用体外培养正常羊驼皮肤黑素细胞,检测不同浓度α-MSH（0、10-9、10^-8、10^-7moL／L）对羊驼皮肤黑素细胞中诱导型一氧化氮合酶（NOS2）基因...  相似文献   

植物乳杆菌α-半乳糖苷酶基因（melA）的克隆与序列分析   总被引：1，自引：1，他引：0  

任大勇  李昌  秦艳青  杜寿文  胡博  金宁一 《中国畜牧兽医》2011,38(1):95-99

试验以L.plantarum1.557的基因组DNA为模板,通过PCR技术扩增α-半乳糖苷酶基因（melA）基因,PCR产物经纯化回收后克隆至pMD18-T载体中,转化E.coli DH5α感受态细胞,筛选阳性克隆,提取质粒进行SacⅠ和Sph Ⅰ酶切及PCR扩增鉴定,并对melA基因片段进行序列测定。结果表明,测得的melA基因序列全长2240 bp,含有酶切位点、启动子、SD序列及编码738个氨基酸的开放阅读框,理论分子质量为84 ku;4种核苷酸中GC含量为47.45%、AT含量为52.55%;序列中有3个碱基发生了变化,均为无义突变,未导致其推导的氨基酸的改变;序列测定结果与GenBank中登录的序列进行比较,核苷酸和氨基酸序列的同源性除与AY873840相比分别为96.6%和92.2%外,其余均大于99%。试验成功克隆了melA基因,为进一步构建重组表达载体奠定了基础。  相似文献   

Effect of CMH on Growth and Enzyme Activity in Wheat (Triticum aestivum L.) at Different Growth Stages and Light Intensities     

M. M. El-Fouly  Z. M. Moubarak    M. K. Fouad  A. F. A. Fawzi 《Journal of Agronomy and Crop Science》1990,164(2):110-115

Pot experiments were conducted over 3 successive seasons using wheat Giza 155 grown in normal or low (50 % of normal) light intensity. Concentrations of 50, 100, 500, 1000 or 2500 ppm CMH were sprayed at tillering, early stem extension, late stem extension or heading. Grain weight/plant, specific grain weight, grain number/ear and ear number/plant were increased with lower concentrations of CMH treatments only at the first 2 stages under varied light intensity. During both tillering and early stem extension, CMH spraying increased activities of α-amylase and β-fructofuranosidase in leaves leading to more accumulation of total carbohydrates in grains and consequently more grain production/plant. Such effect occurred with 50–500 ppm CMH under normal light intensity as well as with all concentrations of this compound under low intensity. Meanwhile, 1000 and 2500 ppm CMH resulted in growth inhibition.  相似文献   

α-蒎烯分子中四元环上的亲核加成反应研究   总被引：3，自引：1，他引：2  

刘云派  胡玖林  杜海燕 《福建林学院学报》2003,23(2):111-113

松节油与二硫化碳和氢氧化钠反应,采用2种不同的工艺,可以分别合成萜烯二硫代羧酸钠和萜烯黄原酸钠.并对2种化合物的光谱行为进行了测定.  相似文献   

Einfluß eines variierten N-Angebots auf α-Amylase-Aktivität, primäre Dormanz und Auswuchsresistenz von Weichweizen (Triticum aestivum L.), Roggen (Secale cereale L.) und Triticale (X Triticosecale Wittmack)     

H. Detje  G. Geisler 《Journal of Agronomy and Crop Science》1989,162(3):201-213

Influence of Varying N-Fertilization Rates on α-Amylase Activity, Primary Dormancy and Resistance to Pre-Harvest Sprouting in Wheat ( Triticum aestivum L.), Rye ( Secale cereale L.) and Triticale (X Triticosecale Wittmack)
Pre-harvest sprouting, induced by unfavourable ecological conditions, can affect the grain growers success considerably. Positive correlations are reported between resistance to pre-harvest sprouting and primary dormancy. Genotypes with a short dormancy period have a high pre-harvest sprouting risk. In the case of a premature germination of caryopses in the head of grain a hydrolysis of intact starch granules caused by the endoenzyme α-amylase takes place in the endosperm.
Negative correlations between falling number and protein content are reported, however, it is unknown, if a varying N-application influences pre-harvest sprouting rates, dormancy periods and amylase activity. For this reason, both greenhouse and field trials were conducted with different N-fertilization rates and (additional in the greenhouse) a rain simulation treatment.
High amounts of α-amylase a few days post anthesis are opposed to small enzyme activities in mature kernels. Stratificating temperatures and germination inducing precipitations at the same time are inducing pre-harvest sprouting and a high α-amylase activity especially in rye and triticale. It seems as if N-deficiency reduces the possibility of pre-harvest sprouting, on the other hand high N-rates increase the enzymes' activity and promote germination processes in the kernel. Effects of N-fertilization on dormancy are not known.
In the discussion of reasons for an increase of α-amylase activity in sprouted grain caryopses, changes in the relation of the phytohormones gibberellic acid (promoter of enzyme activities) and abscisine acid are mainly presumed.  相似文献   

Propagation in vitro of pear, Pyrus communis L., cultivars ‘William's Bon Chrétien’, ‘Packham's Triumph’ and ‘Beurré Bosc’     

Xiao-Shan Shen  M.G. Mullins 《Scientia Horticulturae》1984,23(1):51-57

Shoot cultures were established from nodal explants of 3 pear cultivars by subculture on Murashige and Skoog medium containing mixtures of 3 cytokinins. Zeatin and 6·(γ,γ-dimethylallylamino)purine were both supplied at 10 μM and benzyladenine as follows: ‘William's Bon Chrétien’, 6 μM; ‘Packham's Triumph’, 8 μM; ‘Beurré Bosc’, 10 μM. Production of axillary shoots was greater by basal explants (4–6) comprising the remnants of the previous subculture than by apical explants (2–3) comprising the distal parts of extension shoots. Up to 80% of microcuttings of all cultivars formed roots in vitro with either γ-indolebutyric acid, 10 μM, or α-naphthaleneacetic acid, 10 μM, and approximately 50% of rooted microcuttings were successfully established as container-grown plants. Use of aseptic methods for producing own-rooted pears is discussed in relation to fruit growing and fruit improvement.  相似文献