钼镉联合诱导对麻鸭肾功能及肾Bcl-2基因mRNA表达的影响     

《中国兽医学报》2016,(3):490-495

旨在研究钼镉联合诱导对麻鸭肾功能及肾脏中Bcl-2基因mRNA表达量的影响。选用360羽健康的11日龄"江南2号"麻鸭并随机分成6组,每组60只(公母各30羽)。(NH_4)_6Mo_7O_(24)·4H_2O和3CdSO_4·8H_2O分别作为本试验的钼源和镉源,在每千克基础日粮中添加不同剂量的Mo、Cd,各组的处理情况分别为:对照组(Mo 0 mg,Cd 0mg)、低钼组(Mo 15 mg,Cd 0 mg)、高钼组(Mo 100 mg,Cd 0 mg)、镉组(Mo 0 mg,Cd 4 mg)、低钼镉组(Mo 15mg,Cd 4mg)、高钼镉组(Mo 100mg,Cd 4mg)。试验期120d。在试验期第30、60、90、120天时采集血样分离血清用于检测血清中Cr、UA、BUN、Na~+、K~+、Cl-、Mg~(2+)的含量;于60、120d采集鸭肾组织检测Bcl-2 mRNA的表达量。结果显示,120d高钼镉组Cr和BUN显著高于对照组(P0.05);120d试验组血清中UA低于对照组但差异不显著(P0.05);120d试验组血清Na~+、Cl-显著低于对照组(P0.05);试验组血清K~+低于对照组但差异不显著(P0.05);90、120d高钼组和高钼镉组的血清Mg~(2+)显著低于对照组(P0.05);在60、120d,除低钼组外,其余试验组鸭肾中Bcl-2mRNA表达量均极显著低于对照组(P0.01),高钼镉组极显著高于其他组(P0.01);钼镉联合组变化比单独组更显著。此结果表明钼、镉及其联合诱导可导致麻鸭肾功能损伤、肾脏中Bcl-2 mRNA表达量下调,且钼镉呈协同作用。  相似文献   

纳米TiO_2对鸡肉源微生物的光催化抑菌效果研究     

王佳媚  章建浩 《安徽农业科学》2016,(23)

[目的]研究不同作用因素对纳米TiO_2的光催化活性影响,为纳米TiO_2光催化杀菌技术的开发与应用提供参考。[方法]以菌液初始浓度、光照强度和纳米TiO_2浓度为主要变量因素,研究不同因素条件下纳米TiO_2对鸡肉源微生物P.fluorescens和M.caseolyticus的光催化抑菌作用效果。[结果]纳米TiO_2浓度增加对P.fluorescens和M.caseolyticus的抑菌作用呈先升高后降低的趋势,最佳浓度值为0.4 g/L;增高光照强度,降低初始菌液浓度,纳米TiO_2的光催化抑菌效果增强;相同试验条件下,纳米TiO_2对P.fluorescens和M.caseolyticus的光催化抑菌作用一致,对M.caseolyticus的抑菌率始终低于P.fluorescens。[结论]M.caseolyticus对纳米TiO_2的光催化作用比P.fluorescens更加敏感。  相似文献   

传染性法氏囊病病毒主要结构蛋白VP2、VP1和VP2-VP1串联基因的原核表达及其初步应用     

王威威  刘婷  陈果  姬忠华  赵增志  黄宇  陈艳艳  石梦雅  何秀苗  韦平 《畜牧与兽医》2020,(5):73-80

为获得传染性法氏囊病病毒(IBDV)特异性抗体检测用抗原VP2、VP1及VP2-VP1蛋白,分别设计引物扩增IBDV野毒株NN1172的VP2和VP1基因,并扩增VP2和VP1基因中抗原性和亲水性较好的重要区域,通过PCR扩增基因串联方法对截短的VP2和截短的VP1基因进行串联,首次获得VP2-VP1串联基因,并对VP2、VP1和VP2-VP1串联基因进行了原核表达和鉴定。结果成功构建了原核表达载体pET-VP2、pET-VP1和pET-VP2-VP1;诱导表达条件显示,3个重组质粒分别转入BL21菌株后经0.05 mmol/L IPTG诱导表达,分别得到分子量为69、114和63 kDa的VP2、VP1和VP2-VP1重组蛋白,且均以包涵体形式表达,3个重组蛋白分别于诱导后5、3和6 h时表达量最多。Western blot结果显示,表达的VP2、VP1和VP2-VP1蛋白与鸡抗IBDV阳性血清均具有良好的反应原性。以纯化的VP2、VP1和VP2-VP1蛋白作为包被抗原对传染性支气管炎病毒(IBV)、呼肠孤病毒(ReoV)、禽白血病病毒(ALV)和新城疫病毒(NDV)4种阳性血清检测均为阴性,表明所获得的纯化蛋白具有高度的特异性;对免疫了IBD灭活疫苗,IBD基因工程疫苗和IBD弱毒疫苗的商业鸡群进行抗体检测,结果均能显示疫苗免疫后机体抗体水平的变化趋势。本研究表明利用该原核表达系统所表达的3个蛋白均具有良好的免疫反应活性,为IBDV特异性抗体的检测和新型亚单位疫苗的研发奠定基础。  相似文献   

Periplaneta americana peptide decreases apoptosis of pig-ovary granulosa cells induced by H2O2 through FoxO1     

Caihua Kong  Kena Liu  Qin Wang  Rong Fu  Huaxin Si  Shiyan Sui 《Reproduction in domestic animals》2021,56(11):1413-1424

Oxidative stress can induce apoptosis of granulosa cells and lead to follicular atresia, thereby reducing the number of pigs giving birth. The aim of this study was to investigate the protective effect of Periplaneta americana peptide (PAP) on the apoptosis of the granulosa cells of pig ovaries (PGCs) induced by hydrogen peroxide (H₂O₂) via FoxO1. PGCs were treated with H₂O₂ to establish a cell apoptosis model. Cell viability was measured using the cell counting kit-8 (CCK-8) assay, and cell apoptosis was detected using flow cytometry. The malondialdehyde (MDA) level and nitric oxide (NO) content were detected to reflect the oxidative stress. Western blotting, qRT-PCR and overexpression were undertaken to determine the expression of FoxO1 and caspase-3, and immunofluorescence was used to detect FoxO1 in the nucleus and cytoplasm. PGCs were treated with 100 μM H₂O₂ for 6 hr, which resulted in oxidative damage and apoptosis and an apoptosis rate for PGCs of 32.95%. Next, PGCs were treated with 400 μg/ml PAP for 24 hr to repair the apoptosis induced by H₂O₂. PAP improved cell viability in H₂O₂-stimulated PGCs, the increased MDA level and NO content caused by H₂O₂ stimulation were reversed and the apoptotic rate of PGCs was reduced. The qRT-PCR and Western blotting results indicated that PAP decreased the H₂O₂-induced apoptosis and the expression of FoxO1 and caspase-3 in PGCs. The effect of PAP was the same following FoxO1 overexpression. FoxO1 was expressed in the nucleus when stimulated by H₂O₂ or overexpression; however, it migrated to the cytoplasm following PAP treatment. PAP decreased the apoptosis of PGCs induced by H₂O₂ by regulating FoxO1 expression and nuclear translocation.  相似文献   

微生物菌群培养组学在动物医学中的应用     

彭娜  彭先启  乐敏 《畜牧兽医学报》2020,51(12):2942-2953

实验室条件下可培养的微生物约占自然界中微生物总数的1%，这限制了人们对99%未知微生物的认识和利用，而研究表明，那些“不可培养的微生物”是可以被开发和利用的，未能被纯培养的微生物才是未知微生物的主体。微生物培养组学探索利用多种培养条件和长时间的培养，结合基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF-MS）和16S核糖体RNA（rRNA）测序可以大规模鉴定各种微生物，同时利用全基因组测序和宏基因组测序手段对未知微生物进行深入分析。本文综述了国内外近年来微生物菌群培养组学在反刍动物胃肠道、禽类盲肠及家畜鼻腔微生物菌群研究中的最新进展，探讨将动物体内菌群培养组学方法应用于动物疾病防治领域的可行性。作为一个新兴的研究方法，尽管该培养组学还存在一些不够成熟的方面，但它的发展前景十分广阔，微生物菌群培养组学方法和其他研究方法的互补已经逐渐成为发展兽医微生物学新的突破口。  相似文献   

海南兰科植物新记录     

叶康 《热带作物学报》2019,40(11):2261-2263

本文报道了海南兰科植物3新记录种,分别是全唇叉柱兰[Cheirostylis takeoi (Hayata) Schltr]、兰屿芋兰（Nervilia lanyuensis S. S. Ying）和台湾隐柱兰（Cryptostylis taiwaniana Masam）。凭证标本存于上海辰山植物园标本馆（CSH）。  相似文献   

Carbon stocks and carbon fluxes from a 10‐year prescribed burning chronosequence on a UK blanket peat          下载免费PDF全文

G. D. Clay  F. Worrall  N. J. Aebischer 《Soil Use and Management》2015,31(1):39-51

Prescribed burning is a common land management technique in many areas of the UK uplands. However, concern has been expressed at the impact of this management practice on carbon stocks and fluxes found in the carbon‐rich peat soils that underlie many of these areas. This study measured both carbon stocks and carbon fluxes from a chronosequence of prescribed burn sites in northern England. A range of carbon parameters were measured including above ground biomass and carbon stocks; net ecosystem exchange (NEE), net ecosystem respiration (R_eco) and photosynthesis (P_g) from closed chamber methods; and particulate organic carbon (POC). Analysis of the CO₂ data showed that burning was a significant factor in measured CO₂ readings but that other factors such as month of sampling explained a greater proportion of the variation in the data. Carbon budget results showed that whereas all the plots were net sources of carbon, the most recent burn scars were smaller sources of carbon compared with the older burn scars, suggesting that burning of Calluna‐dominated landscapes leads to an ‘avoided loss’ of carbon. However, this management intervention did not lead to a transition to a carbon sink and that for carbon purposes, active peat‐forming conditions are desirable.  相似文献   

油菜种子低亚油酸和亚麻酸含量异交稳定技术的建立     

郎春秀  王伏林  吴学龙  石江华  陈锦清  吴关庭 《核农学报》2016,(9):1716-1721

为降低油菜种子中的亚油酸和亚麻酸含量并使之在异交条件下保持基本稳定,以高油品种CY2作为受体亲本,采用RNA干涉技术沉默油菜内源Bn FAD2基因的表达,抑制油酸脱饱和反应。育成的3个独立转基因株系的亚油酸和亚麻酸含量下降到6%以下,其中亚油酸含量较受体亲本降低78.2%~86.5%,亚麻酸含量降低53.4%~65.8%。将3个转基因株系与2个亚油酸和亚麻酸含量较高的常规品种正反交,F1种子中这2种脂肪酸的含量依然保持在与转基因亲本相仿的低水平。由此可知,通过RNA干涉技术沉默Bn FAD2基因的表达可赋予油菜低亚油酸和亚麻酸含量的异交稳定特性。这一特性的获得对于今后培育异交稳定的高油酸油菜新品种具有重要意义。  相似文献   

The Olsen P/solution P relationship as affected by soil properties     

I. Sánchez‐Alcalá  M. C. del Campillo  V. Barrón  J. Torrent 《Soil Use and Management》2014,30(4):454-462

  相似文献   

中、美学校体育课程设置比较分析*     

袁〓勇  毛伟琴  张〓鹏 《上海交通大学学报(农业科学版)》2014,(5):5-9

运用比较分析等方法，对宾夕法尼亚州和上海市学校体育课程设置进行比较分析发现，美国学校体育课程设置内容广泛，重视个体全面发展、注重人文关怀、注重各级学校体育课程衔接；提出上海市学校体育课程设置要提高衔接性、体育课程设置应该重视人文关怀，关注残疾人体育、充分利用体制优势，促进学生养成良好体育锻炼习惯、加强学校、家庭和社区的联动，构建完整体育活动圈等建议。以期为学校体育课程改革提供些许有益的参考，从而进一步促进学生身心健康。  相似文献