亚洲璃眼蜱差异表达新基因P18的克隆和鉴定     

王玉霞  周金林  周勇志  龚海燕  向飞宇  程天印  刘毅 《中国预防兽医学报》2006,28(4):396-400

为了进行抗蜱和蜱传病疫苗的研究,本实验对亚洲璃眼蜱雌蜱吸血前后唾液腺消减文库中获取的一个全长编码基因P18进行了研究。该基因全长519bp,共编码170个氨基酸,分子量为18.36Ku,等电点为4.28。BLAST分析表明,该基因预测的氨基酸与肩突硬蜱、篦子硬蜱唾液腺的抗凝血小肽有30%～40%低度同源性。将该基因亚克隆到pET-32a( )表达载体,转化BL21(DE3)宿主菌,经IPTG诱导,重组融合蛋白以可溶性形式高效表达。将可溶性重组蛋白免疫小鼠后获得的抗血清。经免疫印迹分析表明,该重组蛋白抗体可特异性的识别半饱雌蜱唾液腺中的天然蛋白抗原,而未吸血雌蜱唾液腺中则不显现特异条带。RT-PCR结果进一步证实,该基因在蜱吸血后的唾液腺中差异表达。  相似文献   

鹅源禽副粘病毒NA-1株HN蛋白基因的遗传变异研究     

王学理  武迎红  陈丽艳  高志刚  丁壮 《中国预防兽医学报》2006,28(5):530-534

鹅副粘病毒分离株NA-1株经11日龄鸡胚增殖后纯化。提取病毒的基因组RNA，采用RT-PCR扩增出与预期设计的1．7kb大小相符合的特异条带。将扩增产物提纯后克隆入pMD18-T载体，经纯化、筛选及酶切鉴定后，初步获得了含鹅副粘病毒HN基因的阳性克隆，并对阳性克隆进行了测序。测序后拼接得出HN基因的全序列长度为1740bp，该基因的ORF总长为1716bp，编码571个氨基酸。与GenBank下载的15株参考毒株比较HN基因编码区全核苷酸序列，发现所测NA-1株与国内标准强毒株F48E9核苷酸的同源性为84．5％，氨基酸的同源性为89．5％；与传统的疫苗株La Sota核苷酸的同源性为82．2％，氨基酸的同源性为88．5％；与鹅源禽副粘病毒SF02株核苷酸的同源性为97．1％，氨基酸的同源性为97．4％；与鹅源禽副粘病毒YG97株核苷酸的同源性为97．3％，氨基酸的同源性97．4％。说明NA-1株和SF02株、YG97株亲缘关系较近，它们三者可能有着共同的来源。根据基因树分析，NA-1株应属于基因VII型新城疫病毒。由此更进一步证实新城疫可以感染水禽，且对鹅具有高度的致死性。  相似文献   

香菇菌种性能衰退的原因及复壮技术     

吴春玲  吴学谦  郑巧平  项寿南  付立忠  吴庆其  魏海龙  李海波  柳青 《中国食用菌》2006,25(5):9-11

从遗传变异与环境因子2个方面分析探讨了引起香菇菌种性能衰退的主要原因,内因主要包括极性发生变异、自交产生的变异、杂交种性不稳定和基因突变；外因主要包括培养条件不适宜、菌种老化、菌种保藏不当和菌株无性繁殖体感染病毒。针对遗传变异和环境条件引起的菌种性能衰退．提出了有效的预防措施及具有可操作性的菌种复壮技术．  相似文献   

Impact of dominance effects on sow longevity   总被引：1，自引：0，他引：1  

T. Serenius  K.J. Stalder  & M. Puonti 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(6):355-361

The purpose of the current study was to estimate variance components, especially dominance genetic variation, for overall leg action, length of productive life and sow stayability until third and fifth parity in the Finnish pig populations. The variance components were estimated in two purebred [Landrace (LR), n = 23 602 and Large White (LW), n =22 984] and crossbred (LR × LW, n = 17 440) data sets. Five different analyses were carried out for all the traits to compare the effect of sows’ inbreeding, common litter environment and parental dominance in the statistical model when determining the genetic correlations of the traits for the two purebred and crossbred populations. Estimated heritabilities for the traits ranged from 0.04 to 0.06. The estimates for the proportion of dominance variance of phenotypic variance (d²) varied between 0.01 and 0.17, and was highest in the crossbred dataset. The genetic correlations of the same traits in purebred and crossbred were all high (>0.75). Based on current results, the effect of dominance should be accounted for in the breeding value estimation of sow longevity, especially when data from crossbred animals are included in the analyses. Because dominance genetic variation for sow longevity exists that variation should be utilized through planned matings in producing sows for commercial production.  相似文献   

The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’)     

Michael W. Bairu  Catherine W. FennellJohannes van Staden 《Scientia Horticulturae》2006

The effect of the type and concentration of plant growth regulators and sub-culturing on somaclonal variation were studied in Cavendish banana cv. ‘Zelig’ obtained from African Biotechnologies Ltd., South Africa. In vitro grown plants at the fourth multiplication cycle were used for the investigation. Auxins (IAA, IBA and NAA) and cytokinins (BA and TDZ) were used to multiply shoots for 10 generations. Bands generated through RAPD-PCR were scored according to whether they were present (1) or absent (0) to determine the extent of somaclonal variation. Results were then analyzed using cluster analysis. The relationship between multiplication rate and somaclonal variation was assessed using correlation analysis. Results indicated that treatments with higher multiplication rates produced more variants; sometimes as high as 72%. Dwarf off-types accounted for 88% of the variation. A dwarf-specific band, about 1500 kb in size, was amplified by the primer OPC-15. The band appeared consistently in normal plants but was absent in all dwarf plants.  相似文献   

鸡新城疫病毒地方株的分离鉴定及其遗传变异分析   总被引：2，自引：0，他引：2  

周静  杜冬华  孙继国 《中国家禽》2007,29(10):8-11

从河北省保定地区发病鸡群中分离到2个具有血凝性的病毒株分别命名为HBA和HBB,其血凝作用可被NDV阳性血清抑制,而不能被AIV(H9亚型)、EDS76阳性血清抑制,表明2个分离株均为新城疫病毒。通过测定MDT、ICPI和IVPI等方法鉴定分离株的毒力符合强毒标准。利用RT-PCR技术成功扩增了2株分离株的F基因片段(约540bp),通过测序及遗传变异分析表明两毒株之间的核苷酸同源性为87.7%,氨基酸同源性为91.6%,结合系统发育进化树分析表明HBA为基因Ⅵ型,HBB为基因Ⅶ型。  相似文献   

科尔沁沙地翁牛特旗气温的非对称变化分析     

王红梅  骆永明  蒋德明 《干旱区研究》2007,24(2):261-267

分析科尔沁沙地内蒙古翁牛特旗1957年以来逐月平均气温、平均最低气温、平均最高气温.结果表明:翁牛特旗近50年平均气温、日最高气温、日最低气温随时间均呈增加态势;日最低气温的增加量是日最高气温的11倍;1月最低气温的线性增加率为8.77 ℃/100 a,7月最高气温的线性增加率为3.12 ℃/100 a,其结果导致日较差减少;20世纪80年代后期年平均气温、日最高气温、日最低气温显著升高.  相似文献   

The potential of flat-bed passive integrated transponder antennae for studying habitat use by stream fishes     

L. A. Greenberg  P. S. Giller 《Ecology of Freshwater Fish》2000,9(1-2):74-80

Abstract – Describing habitat use by stream fishes is important from both basic ecological and fisheries management points of view. The most widely used methods of measuring habitat use vary in degree of effort required, level of intrusiveness and in the level of spatial and temporal resolution. In this paper, we describe a remote monitoring technique that can provide detailed and continuous data on habitat use of individual fish in the field. The technique is based on the passive integrated transponder (PIT) system, in which a newly developed flat-bed antenna is placed on the stream bottom and simply requires a PIT-tagged fish to swim over it. We present data obtained from work using this new technology on brown trout ( Salmo trutta ) in stream enclosures, in which we describe habitat use and temporal patterns of movement by individuals and relate such data to growth rate and sex of the individual fish as well as to pool depth and time of day. In addition, we describe the range of applications of the flat-bed PIT-antenna as well as the advantages and disadvantages of using the system. ^NOTE  相似文献   

汉语“着”的日译浅析     

忽海燕 《山西农业大学学报(自然科学版)》1999,(1)

汉语中的“着”字，是一个常见的多音、多词性、多义的词，出现频率高，使用范围广，因此，把汉语“着”字译成日语时，也就出现了多种不同的译法。本文按照“着”字的汉语读音、词性、词义的顺序，通过实例就它的日译问题进行一番分析。  相似文献   

石河子地区犬细小病毒野毒株的PCR检测与遗传变异初步研究     

张娜  张银国 《现代畜牧兽医》2012,(3):64-66

为探索石河子地区的CPV野毒株是否发生遗传变异,本试验收集石河子地区自然发病犬及病死犬的粪便和组织,提取其病毒基因组,利用PCR技术对犬细小病毒VP2基因片段进行扩增,对扩增片段进行克隆和测序,并与不同地域流行株VP2基因进行比对,分析其差异。结果显示,本试验成功克隆了VP2基因,且序列分析结果显示一毒株存在14个点突变位点其和5个缺失位点,另一毒株存在7个突变位点。经基因型鉴定,野毒株的基因型均为CPV-2a型。  相似文献