Cloning and Expression of Gene Responsible for High-Tillering Dwarf Phenotype in Indica Rice Mutant gsor23     

YUAN Shou-jiang  WANG Tao  YIN Liang  ZHAO Jin-feng  WAN Jian-min  LI Xue-yong 《水稻科学》2013,20(5):320-328

High-tillering dwarf mutant gsor23 was generated from an indica rice variety Indica9 radiatied by γ-ray. Genetic analysis showed that this phenotype was controlled by one single recessive gene, which was mapped within a physical distance of 386 kb between two insertion-deletion (InDel) markers C1-WT2 and C1-WT4 on the long arm of chromosome 1. There is a known gene D10 within this region, the mutation of which causes high-tillering in rice. Sequence analysis of the D10 allele in gsor23 revealed that the base cytosine (C) at the 404^th position in the coding region was deleted, which would cause frameshift mutation after the 134^th amino acids. The mutation site and indica background of gsor23 were different from the previously reported japonica mutants d10-1 and d10-2. Therefore, gsor23 is a novel allelic mutant of D10 which encodes the carotenoid-cleaving dioxygenase 8 (CCD8), a key enzyme involved in the biosynthesis of the new plant hormone strigolactones (SLs). After treatment with GR24, a synthetic analogue of SLs, the high-tillering phenotype of gsor23 was restored to normal. Real-time RT-PCR analysis showed that D10 expression was high in roots, but low in leaves. Compared with the wild type Indica9, the expression of the SL biosynthesis gene D10 was upregulated, while genes likely involved in the SL signal transduction pathway such as D3 and D14 were down-regulated in the gsor23 mutant.  相似文献   

黑化雉鸡羽色遗传的研究     

王峰  葛明玉 《经济动物学报》1997,1(3):34-38

通过黑化雉鸡 (Melanisticmuckedpheasant)和大型环颈雉鸡 (Jumboringneckedpheas ant)的正交和反交 ,以及F1 中进行自交 ,观察F1 和F2 中羽色性状的表现和分离比例。试验结果表明 ,黑化雉鸡羽色性状对于环颈雉鸡羽色的遗传方式是显性遗传 ,而且显性是完全的 ;黑化雉鸡羽色性状也由常染色体上一对等位基因M来控制的 ,并不存在显性致死现象  相似文献   

伪狂犬病病毒Bartha株TK-/LacZ+突变株的构建及其生物学特性研究   总被引：3，自引：1，他引：2  

刘正飞  陈焕春  周复春  方六荣  何启盖 《畜牧兽医学报》2002,33(3):304-307

本研究将伪狂犬病病毒Bartha株基因组与含有LacZ标志基因的TK基因转移质料pUEKPZ共转染猪肾传代细胞PK－5，细胞出现病变后，反复冻融3次收毒，按1：5稀释接种于IBRS－2细胞。在X－gal存在下挑取蓝斑，蓝斑筛选3次，再进行空斑试验，同时用PCR扩增LacZ基因，经3次空斑纯化，随机挑取的空斑均能扩增出LacZ基因，证实所获得的重组病毒为伪狂犬病病毒Bartha株TK^-/LacZ^ 突变株。TCID50试验表明，TK失活对Bartha株在细胞上增殖无影响；Balb/C小鼠试验表明，该突变对Balb/C小鼠的安全性明显高于Bartha亲本毒株。  相似文献   

番茄对晚疫病的株龄相关抗性及Ph-3的抗性机制     

李涛  刘磊  郑峥  杜永臣  李君明 《园艺学报》2015,42(6):1077-1084

目前已明确番茄抗晚疫病R基因表现明显的株龄相关抗性(Age-related Resistance,ARR),但抗晚疫病QTL的抗性规律尚不明确。以携带抗晚疫病基因Ph-3的栽培种番茄(Solanum lycopersicum)‘CLN2037B’和野生种醋栗番茄(S.pimpinellifolium)‘L3708’以及易感病材料栽培种番茄(S.lycopersicum)‘LA2818’为对照,对含有抗晚疫病QTL的多毛番茄(S.habrochaites)‘LA2099’、‘LA1033’和‘LA1777’是否也存在株龄相关抗性进行了分析。结果表明,携带抗晚疫病QTL的3个材料的6叶期和9叶期植株病情级数均比3叶期明显降低,表明QTL抗性与株龄相关。利用乙烯、水杨酸和茉莉酸素合成或缺失的突变体和病毒诱导基因沉默(Virus Induced Gene Silencing,VIGS)技术,初步明确了乙烯和水杨酸参与6叶期Ph-3基因介导的对晚疫病的抗性,而茉莉酸不参与。  相似文献   

Inheritance of high palmitic acid content in the sunflower mutant CAS-12 and its relationship with high oleic content     

B. Pérez-Vich  R. Garcés  J. M. Fernández-Martínez 《Plant Breeding》2002,121(1):49-56

CAS‐12 is a sunflower mutant with increased levels of palmitic (C16: 0 = 30%) and oleic (C18: 1 = 55%) acids in its seed oil, hence it has a reduced linoleic acid content (C18: 2 < 5%). This study was conducted to determine the inheritance of high C16: 0 content and its relationship with high C18: 1 content in CAS‐12. Reciprocal crosses involving CAS‐12, CAS‐5 (high C16: 0 content), HAOL‐9 (high C18: 1 content) and HA‐89 (standard fatty acid profile) were made. The F₁, F₂ and BC₁F₁ generations were obtained. The genetic control of the high C16: 0 trait in CAS‐12 was partially recessive and gametophytic. In all cases, this character segregated in the ratio 19: 38: 7 (low: intermediate: high C16: 0 content) in the F₂ generation. These results, together with the lack of segregation for C16: 0 content in crosses between CAS‐12 and CAS‐5, indicated that the genetic control of the high C16: 0 trait in CAS‐12 was similar to that in CAS‐5 in being controlled by partially recessive alleles (p1, p2, and p3) at three loci. Crosses between HA‐89 and CAS‐12, and HAOL‐9 and CAS‐5 (segregating for C16: 0 and C18: 1) demonstrated that the high C16: 0 and the high C18: 1 traits were independently inherited. However, C18: 1 segregation in these crosses exhibited reversal of dominance. Apparently, the low C18: 1 parental lines carried modifier genes causing the deviation.  相似文献   

Production of a Trisomic Series in Hordeum vulgare cv. 'Golden Promise' and its Use in Locating a Recessive Chlorina Gene   总被引：1，自引：0，他引：1  

R. A. Pickering 《Plant Breeding》1991,106(4):342-344

A trisomic series was produced from a triploid plant of barley (Hordeum vulgare L. cv. ‘Golden Promise’) derived from tissue culture. Its characteristics are briefly described and compared with two trisomic series reported previously. Trisomic number 1 performed poorly under glasshouse conditions. Number 2 failed to set any seed after selfing and must be maintained by pollinating with ‘Golden Promise’. The series was subsequently used to locate a recessive chlorina gene on barley chromosome 3.  相似文献   

Inheritance of a plant type mutant and its utilization in chickpea     

J. S. Sandhu  H. S. Brar  M. M. Verma 《Euphytica》1990,48(2):111-112

Summary A macro-mutant, E 100Y(M) in chickpea (Cicer arietinum L.) was found to affect several plant and seed characters. For plant type monogenic inheritance was observed. A single pair of recessive genes pt/pt was ascribed to this mutant. The mutant locus seemed to be exerting pleiotropic action. The utilization of this mutant for chickpea improvement has been discussed.  相似文献   

Genetical control of alkaloid production in Lupinus mutabilis and the effect of a mutant allele Mutal isolated following chemical mutagenesis     

Watkin Williams  Jill E. M. Harrison  Sumithra Jayasekera 《Euphytica》1984,33(3):811-817

Summary A new mutant allele, described here as mutal, which reduces the alkaloid content in dry matter of lupinus mutabilis has been identified following seed treatment with ethyl methane sulphanate. The allele, when homozygous, reduces the alkaloid content from levels of > 2.0 per cent found in seed dry matter of existing populations to 0.2–0.3 per cent and produces plants with vegetative and seed tissues that are organoleptically sweet. Component alkaloids in plants homozygous for mutal differ in respect of the percentage composition of sparteine and of lupanine, as well as possibly of oxa-sparteine and 4-hydroxylupanine, but none is eliminated in genotypes homozygous for the mutant allele. Alkaloid concentration, in so far as not under the control of mutal, has low heritability but lines were isolated following two generations of successive selection which possessed reduced alkaloid levels. These were interpreted to have arisen as a result of recombination of alleles affecting reduction in alkaloid level at several loci. the mutant, mutal, has been established in a pure breeding line which represents a crucial additional step in the evolution of Lupinus mutabilis towards full status as a crop plant.  相似文献   

若干玉米胚乳突变体基因效应的研究   总被引：3，自引：0，他引：3  

夏涛  刘纪麟 《作物学报》1997,23(6):753-758

对５个玉米单隐性胚乳突变体、３个双隐性胚乳突变体的可溶性糖（还原糖、蔗糖）、水溶性多糖、淀粉、粗蛋白、赖氨酸含量测定，并对突变体种子结构扫描电镜观察。分析了玉米胚乳突主体的基因效应及其互作效应。  相似文献   

A shattering-resistant mutant of Papaver bracteatum Lindl.: characterisation and inheritance     

Arieh Levy 《Euphytica》1985,34(3):811-815

Summary Papaver bracteatum is a potential crop for the codeine industry. Seed shattering prevailing in the species is a major problem which reduces the seed yields. A spontaneous mutant with capsules having no pores of dehiscence was isolated in an experimental field. The seed yields of this shattering-resistant mutant are lower than in plants with normal capsules but the thebaine content of the capsules from the mutant is higher. The mutation is monogenic dominant; it can be used for breeding cultivars for dual purpose-thebaine and seeds.  相似文献