Co-inoculation of selected nodule endophytic rhizobacterial strains with Rhizobium tropici promotes plant growth and controls damping off in common bean     

Linnajara DE VASCONCELOS MARTINS FERREIRA  Fernanda DE CARVALHO  J&#;lia FONSECA COLOMBO ANDRADE  Damiany P&#;DUA OLIVEIRA  Fl&#;vio Henrique VASCONCELOS DE MEDEIROS  Fatima Maria DE SOUZA MOREIRA 《土壤圈》2020,30(1):98-108

Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia solani.However,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase production.In this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield levels.The aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean plants.Greenhouse experiments were conducted under axenic conditions using the common bean cultivar Pérola.The first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of R.solani.The second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common bean.Co-inoculation of the strains UFLA 02-281/03-18(Pseudomonas sp.),UFLA 02-286(Bacillus sp.),and UFLA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping off.For the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UFLA 02-281/02-286/02-290/02-293.Nodule endophytes UFLA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial inoculation and protection against disease.  相似文献   

Autochthonous halotolerant plant growth-promoting rhizobacteria promote bacoside A yield of Bacopa monnieri (L.) Nash and phytoextraction of salt-affected soil     

Umesh PANKAJ  Durgesh Narain SINGH  Pooja MISHRA  Pooja GAUR  C. S. Vivek BABU  Karuna SHANKER  Rajesh Kumar VERMA 《土壤圈》2020,30(5):671-683

Phytoremediation is a promising approach for reclamation of salt-affected soil. Phytoextraction is the most commonly used process, which exploits plants to absorb, immobilize, and accumulate salt in their shoots. In this study, halotolerant plant growth-promoting rhizobacteria (PGPR) were isolated from the rhizosphere of wild grasses growing naturally in salt-affected areas of Lucknow, Uttar Pradesh (India) and were tested for their efficacies of salt-tolerance and plant growth-promoting (PGP) abilities. Based on 16S rRNA sequences, the most efficient halotolerant isolates possessing PGP traits were identified as Pseudomonas plecoglossicida (KM233646), Acinetobacter calcoaceticus (KM233647), Bacillus flexus (KM233648), and Bacillus safensis (KM233652). Application of these isolates as bio-inoculants significantly (P < 0.05) increased the growth and bacoside A yield of a medicinal plant, Bacopa monnieri (L.) Nash, grown on natural salt-affected soil. The phytoremediation of salt-affected soil was evident by the substantial increase in shoot Na⁺:K⁺ ratio of bio-inoculant-treated plants. When compared to un-inoculated control plants, the soil physico-chemical properties of bio-inoculant-treated plants were improved. The shoot and root biomass (fresh and dry weights), soil enzymes, and soil nutrient parameters showed significant positive correlations with the shoot Na⁺:K⁺ ratio. Consequently, the halotolerant PGPR screened in this study could be useful for the reclamation of saline soils concomitant with improved plant growth and bacoside A yield.  相似文献   

四川省毒害植物——紫茎泽兰调查报告   总被引：32，自引：2，他引：30  

周俗  谢永良 《四川草原》1999,(2):39-42

紫茎泽兰是恶性毒害植物,已传入攀枝花市和凉山州两地１４县３区１市,分布面积３７５１６０ｈｍ２,每年以２０～３０ｋｍ的速度向北向东传播。其繁殖能力和侵占性极强,是农、林、牧业生产的大敌,应采取切实可行的方法,发动群众,人人动手,把防除与开发利用结合起来,开展综合防治。  相似文献   

Relationships of Spectral Reflectance with Plant Tissue Mineral Elements of Common Bean (Phaseolus Vulgaris L.) Under Drought and Salinity Stresses     

Blagoja Boshkovski  Constantinos Tzerakis  Georgios Doupis  Anhelina Zapolska  Chariton Kalaitzidis 《Communications in Soil Science and Plant Analysis》2020,51(5):675-686

ABSTRACT

Salinity and drought stresses are critical for Phaseolus vulgaris L. growth and development. They affect plants in various ways, including tissue mineral element content. Micro- and macro-elements in leaves of Phaseolus vulgaris L. (cv. ‘Blue lake’ and cv. ‘Terli’) subjected to deficit irrigation and salinity treatments were investigated, both analytically and with regards to their effect on the leaves’ spectral reflectance. B (boron), K (potassium), Mn (manganese), Na (sodium), Si (silicon) and Zn (zinc) appeared to be influenced by stress factors, mainly responding to salinity increase. The leaf spectral reflectance of the plants appeared to be significantly correlated with most of the elements under investigation. Multivariate regression identified a relationship of the reflectance at particular regions of the spectrum with phosphorus and NDVI (normalized difference vegetation index) and indicated a significant correlation with B, Fe (iron), K, Mn, P (phosphorus) and Zn. Moreover, customized spectral indices, exhibiting significantly high correlation with B, Fe, K, Mg (magnesium), Mn, Na, P, Zn and N (nitrogen), were developed.  相似文献   

汉中市野生兰科植物资源现状及保护开发策略   总被引：2，自引：0，他引：2  

袁海龙 《北方园艺》2011,(9):100-102

汉中市共分布野生兰科植物34属57种,分别占到陕西省的87.8%和68.3%,具有较高观赏价值的有兰属中的春兰和惠兰、独蒜兰属的独蒜兰,具有较高药用价值的有传统贵重中药天麻、白芨等。汉中市野生兰科植物总体资源量大、分布广,有着悠久的兰科植物栽培和开发利用历史,但近年来由于盗挖滥采现象较为严重,建议加强保护,建立野生兰科植物基因库,并加强人工栽培进行开发性的保护。  相似文献   

植酸酶对3种植物性原料中植酸磷含量的影响   总被引：6，自引：0，他引：6  

曹玲  王卫民  杨承泰  杨毅 《淡水渔业》2007,37(4):52-56

利用微生物植酸酶分别对3种植物性原料(豆粕、玉米蛋白粉、小麦)进行酶处理,研究不同浓度植酸酶作用下3种植物性原料中植酸磷含量的变化。3种原料分别有6组处理(植酸酶对照0 U/kg,柠檬酸对照,500U/kg,750 U/kg,1000 U/kg,1500 U/kg),每组处理3个重复。结果表明:植酸酶能够显著降低3种植物性原料中植酸磷的含量(P<0.05),同时提升有效磷的含量。通过对磷当量的分析,本试验中以植酸酶组1000 U/kg最为经济有效。植酸酶对照组中植酸磷含量也有所下降,说明柠檬酸单独对植酸起到了一定的分解作用。  相似文献   

玉米行间导航线实时提取   总被引：10，自引：7，他引：3  

杨洋  张博立  查家翼  温兴  陈黎卿  张铁  董祥  杨学军 《农业工程学报》2020,36(12):162-171

针对高地隙植保机底盘玉米田间植保作业压苗严重的现象,该研究提出了基于车轮正前方可行走动态感兴趣区域(Region of Interest,ROI)的玉米行导航线实时提取算法。首先将获取的玉米苗带图像进行像素归一化,采用过绿算法和最大类间方差法分割玉米与背景,并通过形态学处理对图像进行增强和去噪;然后对视频第1帧图像应用垂直投影法确定静态ROI区域,并在静态ROI区域内利用特征点聚类算法拟合作物行识别线,基于已识别的玉米行识别线更新和优化动态ROI区域,实现动态ROI区域的动态迁移;最后在动态ROI区域内采用最小二乘法获取高地隙植保机底盘玉米行间导航线。试验表明,该算法具有较好的抗干扰性能,能够很好地适应较为复杂的田间环境,导航线提取准确率为96%,处理一帧分辨率为1 920像素×1 080像素图像平均耗时97.56 ms,该研究提出的算法能够为高地隙植保机车轮沿玉米垄间行走提供可靠、实时的导航路径。  相似文献   

夹茎式番茄钵苗取苗机构设计与试验   总被引：1，自引：1，他引：0  

李华  马晓晓  曹卫彬  李树峰  周文静 《农业工程学报》2020,36(21):39-48

为避免取苗机构在夹取钵苗过程中对钵体与根系造成损伤,该研究针对夹茎取苗方式,提出一种基于二阶椭圆齿轮行星轮系以及凸轮摆杆机构的夹茎式番茄钵苗取苗机构。依据二阶椭圆齿轮传动特性与机构工作原理,建立机构运动学理论模型,并对凸轮轮廓曲线进行设计,结合番茄钵苗取苗作业要求及机构特点,基于MATLAB软件开发机构分析软件对机构参数进行优化,并进一步分析优化后取苗轨迹与凸轮各工作段的对应位置关系,建立了夹茎式取苗机构虚拟样机模型,利用ADAMS软件对取苗机构运动过程进行仿真分析,验证机构参数优化结果及零部件结构设计的准确性与合理性。试制取苗机构物理样机开展高速摄影试验,通过对比分析实际工作轨迹与理论分析及仿真轨迹的一致性,验证了取苗机构设计的正确性。搭建自动取苗试验台进行取苗试验。试验结果表明,取苗机构工作性能可靠、稳定,取苗频率为80株/min时,取苗成功率为92%,投苗成功率为94.2%,伤苗率为2.9%。研究结果可为番茄钵苗全自动移栽机自动取苗机构的研发提供参考。  相似文献   

多维贫困视角下喀斯特区贫困乡村空间分异与地域类型划分   总被引：2，自引：1，他引：1  

赵榕  熊康宁  陈起伟 《农业工程学报》2020,36(18):232-240

喀斯特地区贫困程度深且致贫原因复杂,科学划分喀斯特贫困乡村地域类型并提出差异化振兴对策,是喀斯特贫困地区的现实需要。该研究通过构建喀斯特贫困乡村地域系统评价指标体系,对贫困村主导致贫因素进行分级与空间排列组合,划分喀斯特贫困乡村地域类型,提出喀斯特贫困乡村的振兴策略。结果表明：1）喀斯特贫困乡村的空间分异主要受地势起伏度、坡度、耕地比例和人均纯收入等的影响,贫困程度与贫困聚集规模均呈现出南高北低的空间格局。2）喀斯特贫困乡村可划分为：弱综合制约型、强综合制约型、单致贫维度制约型、双维度制约型和三维度制约型贫困村。3）生态脆弱性在喀斯特贫困乡村地域分异中起决定性作用,经济基础薄弱是喀斯特贫困乡村的共性特点,生产资源禀赋与区位交通条件起重要作用。该研究能够为喀斯特贫困乡村减缓相对贫困的模式与路径设计及乡村振兴战略实施提供理论支撑。  相似文献   

利用最小水分限制范围评价东北黑土区免耕和垄作的土壤水分稳定性   总被引：2，自引：1，他引：1  

刘淑珍  高伟达  任图生 《农业工程学报》2020,36(10):107-115

为明确耕作方式对黑土土壤水分稳定性的作用,提高黑土区雨养农业对气候变化的适应性,该研究基于黑土区长期免耕定位试验,利用最小水分限制范围(Least Limiting Water Range, LLWR)评价免耕(NT)和垄作(CT)管理下土壤含水率有效性及其变异特征。结果表明:1)在0～5、5～10、10～20和20～40 cm 4个土层中,NT处理显著降低了5～10 cm的LLWR,其他3个土层LLWR差异均不显著;2)在平水年(2014)、枯水年(2015)和丰水年(2016),NT管理下作物生育期内0～40 cm平均土壤含水率正常率分别为48%、72%和85%,年间变异系数为0.23;CT的土壤含水率正常率分别为56%、20%和51%,年间变异系数为0.38;3)在丰水年,NT与CT的平均有效储水量差值最小,NT比CT高8.95mm;在枯水年相差最大,NT的平均有效储水量比CT高13.99mm。因此,NT管理下土壤水分更稳定地分布在LLWR内,在极端降雨年份(枯水年和丰水年)优势尤其明显。  相似文献