家兔HSL基因多态性及其与生产性状关联性分析   总被引：1，自引：0，他引：1  

邝良德  任永军  谢晓红  雷岷  李丛艳  郑洁  郭志强  张翔宇  张翠霞  杨超 《中国畜牧杂志》2018,(1)

本实验采用PCR-SSCP方法对齐卡巨型白兔和齐兴肉兔HSL基因外显子1的遗传多态性进行研究,并进一步分析HSL基因对生产性状的遗传效应。结果表明:HSL基因外显子1第784位处发生了碱基突变(A→C),共出现AA、AB和BB 3种基因型,在2个品种兔群体中AA型均为优势基因型,A均为优势等位基因;卡方适合性检验表明,2个品种兔群体均显著偏离哈代-温伯格平衡(P0.05);在齐卡巨型白兔中,HSL基因AA型和AB型个体的宰前活体重均显著高于BB型个体(P0.05),AA、BB型个体的全净膛屠宰率和半净膛屠宰率均显著低于AB型个体(P0.05);在齐兴肉兔中,HSL基因AA型个体的宰前活体重显著高于AB、BB型个体(P0.05),BB型个体的全净膛屠宰率和半净膛屠宰率均显著低于AA、AB型个体(P0.05);在2个品种兔群体中,不同基因型个体的滴水损失和剪切力差异均不显著(P0.05)。  相似文献   

4个绵羊品种Leptin基因部分片段的SNPs多态性与生长发育性状的相关性分析   总被引：3，自引：0，他引：3  

黄丹丽  陈仁金  杨章平  毛永江  李云龙  田黛君  陈亮  赵晓勇 《畜牧兽医学报》2008,39(12)

利用PCR-SSCP技术对萨福克、陶赛特、得克塞尔及滩羊4个绵羊品种358个个体Leptin基因等2、3外显子进行多态性分析,共检测到7个SNPs,其中新发现5个SNPs。测序结果表明,在外显子2上无突变。内含子2上存在A99G、G115A、C150T、C171T位点。外显子3上,存在G271A;C316A;G387T位点。外显子3上的SNPs使编码的氨基酸发生变化。统计分析表明A99G、C150T和A99G＋C150T位点与生长发育性状存在相关性。在A99G位点,Aa基因型初生质量、日增质量、体高、胸围和尻宽指标上均高于AA基因型,初生质量、日增质量和体高指标差异显著（P〈0．05）,胸围和尻宽指标差异极显著（P〈0．01）。C150T和A99G＋C150T位点结果一致,突变基因型日增重、体高、体长、胸围和尻宽指标均高于野生基因型,差异显著（P〈0．05）。  相似文献   

Rapid detection of SNPs in candidate genes regulating the growth of orange‐spotted grouper,Epinephelus coioides (Hamilton, 1822), using semiconductor sequencing          下载免费PDF全文

Liang Guo  Junhong Xia  Sen Yang  Mingming Li  Xinghan Chen  Haoran Lin  Zining Meng 《Aquaculture Research》2017,48(2):494-504

Orange‐spotted grouper (Epinephelus coioides) is one of the most important marine food species in Southeast Asia and China and has been cultured for decades. In this study, we fully utilized the limited capacity of semiconductor sequencing, the high efficiency of long‐range PCR for target enrichment and a non‐indexed pooling strategy to screen single‐nucleotide polymorphisms (SNPs) in a breeding population of orange‐spotted grouper. Forty‐one genomic DNA fragments, with a total length of approximately 180 kb, including 22 candidate genes that control growth, and from a DNA pool of 20 heaviest and 22 lightest individuals of the sampled population were successfully sequenced using an Ion Torrent Personal Genome Machine. 3 503 466 clear reads were produced with a length of 192 ± 56 bp, 86.8% of which were mapped to the reference with an average coverage depth of 2567‐fold and physical coverage of 98.8%. Finally, 1623 high‐quality SNPs were adopted. Compared with Sanger sequencing of three random common regions, the sensitivity and specificity of our approach were 39.4% and 100.0% respectively. A mutation located at the third position of the previously labelled start codon of growth hormone receptor type 1 invalidated the start codon. Furthermore, comparison of the frequencies of genotypes and alleles of this site between the two extreme groups, prediction of signal peptide and identification of conservative mRNA sequences suggested that the functional start codon is likely located at the position of another downstream in‐frame ATG in the mutant. These detected SNP markers will provide important tools for the selective breeding of orange‐spotted grouper.  相似文献   

Characterization of single‐nucleotide polymorphism markers in the Mediterranean mussel,Mytilus galloprovincialis     

Manuel Vera  Belén G Pardo  Ania Pino‐Querido  José Antonio Álvarez‐Dios  José Fuentes  Paulino Martínez 《Aquaculture Research》2010,41(10):e568-e575

The Mediterranean mussel, Mytilus galloprovincialis, is one of the most important aquaculture species in Europe. Appropriate molecular markers are required to evaluate genetic resources and to trace genealogies in breeding programmes for improving mussel culture. Microsatellites have been commonly applied to this purpose in other species. However, Mediterranean mussel microsatellites have demonstrated high frequencies of null alleles that hamper accurate estimates of population parameters and confident parentage inferences. As alternative markers, we have characterized in silico 25 potential single‐nucleotide polymorphism (SNP) markers in the Mediterranean mussel from expressed sequence tag (EST) public databases. The genotyping of SNPs was performed using a single‐base extension approach. Their polymorphism was evaluated in 47 individuals from an Atlantic population. Out of the 25 potential SNPs tested, 12 were technically feasible (producing a single amplicon) and polymorphic. All were biallelic and had an unbiased heterozygosity ranging from 0.160 to 0.504. One SNP was from a mitochondrial gene. The combined potential of nuclear SNPs for parentage assignment gave an exclusion probability of a false couple of parents of 0.9471. These markers will be useful for evaluating resources and tracing genealogies in genetic breeding programmes implemented to solve the main problems of mussel culture.  相似文献   

中国美利奴羊OLA-DQB1基因exon2遗传多态性与布鲁氏菌病易感性的关联分析     

王文文  许万云  胡梦薇  李建华  刘朋涛  高剑峰 《中国畜牧兽医》2015,42(6):1495-1503

本试验采用PCR-SSCP方法对148只布鲁氏菌阴性和60只布鲁氏菌阳性中国美利奴羊白细胞表面抗原DQB1(OLA-DQB1)基因exon 2单核苷酸多态性(SNPs)进行了检测,之后挑选不同等位基因进行PCR产物测序,旨在确定该基因的多态性位点,并对每个SNP位点的等位基因频率、基因型频率进行统计分析,从而分析其多态性与布鲁氏菌病易感性的相关性.测序结果表明,在270 bp的序列内共检测到43个SNPs,其中G196A位点的等位基因频率在病例组和对照组中的分布存在极显著差异(P< 0.01),其基因型频率存在显著差异(P< 0.05);C211T位点的等位基因频率在病例组和对照组中存在显著差异(P< 0.05).由此表明,OLA-DQB1基因exon 2多态性与中国美利奴羊布鲁氏菌病易感性呈显著相关.  相似文献   

牙鲆GH基因的SNPs与生长性状关系的初步研究     

王桂兴  刘永新  王玉芬  姜秀凤  刘海金 《中国水产科学》2015,22(2):347-352

以雌核发育牙鲆(Paralichthys olivaceus)为对象,根据Gen Bank收录的牙鲆生长激素基因序列(Gen Bank登录号:D29737)设计9对引物,采用直接测序的方法对50尾雌核发育牙鲆生长激素基因编码区和启动子进行了单核苷酸多态位点(SNPs)筛选,共获得有效序列1 838 bp,启动子区117 bp,内含子区1 050 bp,外显子区671 bp,覆盖牙鲆生长激素基因78.3%的序列。共检测到7个SNPs,平均发生频率为0.38/100个碱基,其中颠换型3个,插入型2个,缺失型2个;内含子区4个(Intron I:C477T、1 091~1 092/insert T、1 129~1 130/insert A;Intron IV:1 906A/-del),外显子区3个(Exon V:2067T/-del、A2006C、A1974G);SNPs与生长性状相关分析结果显示:C477T和2 067T/-del两个位点对牙鲆的体重、体长、体高等生长性状均有显著影响(P0.05),其他5个SNPs对牙鲆生长性状均无显著影响(P0.05)。研究结果可为牙鲆生长性状的SNPs标记辅助选育提供基础数据。  相似文献   

小麦条锈菌3个看家基因SNP引物的首次报道     

李明菊  陈万权  段霞瑜  刘太国  高利  刘博 《植物病理学报》2014,44(5):536-541

In order to analyze the population structure of Puccinia striiformis f. sp. tritici (Pst), SNPs primers of Pst were developed from DNA sequences of nine house-keeping genes Chs, Act, Mapk1, Tub, Cdc2, Rd, Sm, Ls and Ef-1α obtained from GenBank. Eight of which were from Pst and one from P. graminis f. sp. tritici (Pgt). Thirteen pairs of primers were designed and screened based on at least 30 isolates of Pst obtained from diverse locations. Three of them were polymorphic namely Map1351S/Map1683A, Cd28S/Cd352A and Ef137S/Ef531A. Polymorphic loci analysis based on 149 Pst isolates from 5 provinces indicated that the three primers had good polymorphism. Cd28S/Cd352A had 8 polymorphic loci, three of them were phylogenetically informative. Ef137S/Ef531A had 6 polymorphic loci and 4 of them were informative. Map1351S/Map1683A had 8 polymorphic loci and 4 were informative. The 3 primers were used for analyzing Pst population and revealed the ancestral origin, phylogeny relation of haplotypes, genetic differentiation of the population, gene flow, and the evolution and migration relation between Pst populations thereby. The findings indicate that the 3 genes SNP primers can be used for Pst population genetic structure analysis.  相似文献   

巢湖鸭生长素基因和垂体特异性转录因子1基因的PCR-SSCP检测     

LI Jun-ying  李俊营  詹凯  许月英  王德圣  赵瑞宏  唐中节 《中国畜牧兽医》2009,36(5):107-111

以生长素基因Ghrelin和垂体特异性转录因子1 Pit-1基因为候选基因,采用PCR-SSCP和DNA测序技术检测2个候选基因在巢湖鸭群体中的单核苷酸多态性(SNPs)。结果表明,Ghrelin基因exon 3第54 bp位置有G→A碱基的点突变,在巢湖鸭群体中检测到AA、AB、BB 3种基因型,A等位基因频率为0.49,B等位基因频率为0.51;在exon 5第149位和166位发生了C→A和G→T的突变,检测到MM、MN、NN 3种基因型,M等位基因频率为0.19,N等位基因频率为0.81。Pit-1基因的2对引物的扩增片段均未检测到多态性,说明所检测的Pit-1基因外显子3和4序列比较保守。  相似文献   

Stearoyl-CoA desaturase 1 3'UTR SNPs and their influence on milk fatty acid composition of Canadian Holstein cows     

P.M. Kgwatalala  E.M. Ibeagha-Awemu  J.F. Hayes  & X. Zhao 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2009,126(5):394-403

Stearoyl-CoA desaturase 1 (SCD1) catalyses the synthesis of conjugated linoleic acid (CLA) and mono-unsaturated fatty acids (MUFA) in the mammary gland of ruminant animals. Considerable variations in CLA and MUFA have been reported among animals of the same contemporary group. We hypothesized that single nucleotide polymorphisms (SNPs) in the 5' and 3' untranslated regions (UTRs) of the SCD1 gene would influence the production of SCD1 enzyme and consequently its activity in the mammary gland, which may account for some of the observed within breed variations in CLA and MUFA. The 5' and 3'UTRs of the SCD1 gene of 46 Holsteins and 35 Jerseys were analysed for SNPs by sequencing. No SNPs were identified in the 5'UTR, while 14 SNPs were identified in the 3'UTR region. Further analysis revealed three haplotype structures or regulatory variants in Holsteins: named H1, H2 and H3 and only H1 and H3 in Jerseys. An IRES motif was found in the H1 variant. A subsequent association study involving the milk fatty acid profiles of 862 Holstein cows found the H1 regulatory variant to be associated with higher C10 and C12 desaturase indices and consequently with higher contents of C10:1 and C12:1 relative to the H3 variant. The effects of the H2 variant were intermediate to those of H1 and H3. SNPs in the 3'UTR of the SCD1 gene could therefore explain some of the within-breed variations in MUFA content of milk fat.  相似文献   

猪IGF-Ⅱ基因SNPs及其与部分生产性状的关联分析     

王聪明  张嘉保  任文陟  赵志辉  张国梁  刘继丰  岳续朋  刘利 《中国畜牧杂志》2009,45(9)

以46头健康松辽黑猪为实验材料,对猪IGF-Ⅱ基因进行PCR扩增,采用PCR-SSCP技术结合测序分析了猪IGF-Ⅱ基因在松辽黑猪中的多态性。结果表明:松辽黑猪第9外显子存在第29507位C→A、第29729位A→T、第29731位T→C突变。χ2检验表明,本实验中发现的多态位点均处于Hardy-Weinberg平衡状态。最小二乘分析结果显示,exon9突变位点不同基因型个体平均背膘厚、日增重存在显著差异,由此推测,IGF-Ⅱ基因可能对于猪生产性能具有较大影响或与控制生产性能的主基因连锁,可以尝试将其作为重要的一个分子标记用于猪的育种实践。  相似文献