Complement C3 participates in the development of goose fatty liver potentially by regulating the expression of FASN and ETNK1     

Ya Xing  Cheng Xu  Xiao Lin  Minmeng Zhao  Daoqing Gong  Long Liu  Tuoyu Geng 《Animal Science Journal》2021,92(1):e13527

Non-alcoholic fatty liver disease ( NAFLD ) occurs in humans, domestic animals and poultry. Different from upregulation of complement C3 in human NAFLD, C3 expression is inhibited in goose fatty liver ( GFL ), implying a specific role of C3 in GFL. This study was mainly focused on uncovering the uniqueness of goose liver cells in the regulation of C3 expression and identifying the downstream genes of C3 to improve understanding on the specific role of C3 in GFL. The results showed that C3 expression was inhibited in the liver, muscle and fat tissues of the overfed versus control (normally fed) geese. Oleate and insulin could inhibit C3 expression in goose primary hepatocytes but induce it in mouse primary hepatocytes. A total of 1,123 differentially expressed genes ( DEGs ) were affected by C3 overexpression and were mainly enriched in immune response/inflammation and catabolism-related KEGG pathways. Additionally, the representative downstream genes (FASN and ETNK1) of C3 could mediate the role of C3 in the development of GFL. In conclusion, the suppression of C3 in GFL is at least partially attributed to hyperinsulinemia, hyperlipidemia and uniqueness of goose liver cells. Complement C3 does not only affect hepatic steatosis but also affect inflammation/immune response in GFL.  相似文献   

“一事一议”财政政策实施成效及问题分析     

池爱莲 《福建农业科技》2014,(1):77-79

总结分析了福建省大田县农村“一事一议”财政奖补工作的成效和存在的问题,提出加大政策宣传力度、健全组织领导、优化配置奖补资金、加强部门协作、强化监督管理等措施.  相似文献   

西瓜细菌性果斑病菌基因Aave-3192和Aave-2108的功能研究     

周迎辉  胡方平  蔡学清 《热带作物学报》2016,37(7):1325-1331

从本实验室构建的西瓜细菌性果斑病菌菌株A13的Mini-Tn5转座子突变体库中筛选出2株致病性丧失的突变菌株166和167,亚克隆及测序结果表明,突变株166和167的Tn5插入位点基因编号分别为Aave-3192和Aave-2108。为了进一步明确这2个基因的功能,分别对这2个基因进行互补。生物学试验结果表明,互补菌株恢复对西瓜果实的致病性,且在游动性、群体感应、胞外多糖、生物膜等性状上部分恢复至野生菌株A13的水平,表明这2个基因与该病菌在西瓜上的致病性相关。  相似文献   

Dysferlin and animal models for dysferlinopathy     

K Kobayashi  T Izawa  M Kuwamura  J Yamate 《Journal of toxicologic pathology》2012,25(2):135-147

Dysferlin (DYSF) is involved in the membrane-repair process, in the intracellular vesicle system and in T-tubule development in skeletal muscle. It interacts with mitsugumin 53, annexins, caveolin-3, AHNAK, affixin, S100A10, calpain-3, tubulin and dihydropyridine receptor. Limb-girdle muscular dystrophy 2B (LGMD2B) and Miyoshi myopathy (MM) are muscular dystrophies associated with recessively inherited mutations in the DYSF gene. The diseases are characterized by weakness and muscle atrophy that progress slowly and symmetrically in the proximal muscles of the limb girdles. LGMD2B and MM, which are collectively termed "dysferlinopathy", both lead to abnormalities in vesicle traffic and membrane repair at the plasma membrane in muscle fibers. SJL/J (SJL) and A/J mice are naturally occurring animal models for dysferlinopathy. Since there has been no an approach to therapy for dysferlinopathy, the immediate development of a therapeutic method for this genetic disorder is desirable. The murine models are useful in verification experiments for new therapies and they are valuable tools for identifying factors that accelerate dystrophic changes in skeletal muscle. It could be possible that the genetic or immunological background in SJL or A/J mice could modify muscle damage in experiments involving these models, because SJL and A/J mice show differences in the progress and prevalent sites of skeletal muscle lesions as well as in the gene-expression profiles of their skeletal muscle. In this review, we provide up-to-date information on the function of dysferlin, the development of possible therapies for muscle dystrophies (including dysferlinopathy) and the detection of new therapeutic targets for dysferlinopathy by means of experiments using animal models for dysferlinopathy.  相似文献   

芩连液与白虎汤对气分证家兔补体经典途径活化的影响比较     

张世栋  王东升  王旭荣  李世宏  李锦宇  陈炅然  李宏胜  严作廷 《畜牧兽医学报》2012,43(5):816-820

为比较经方白虎汤与自拟方芩连液的分子疗效区别,以静脉注射内毒素建立了具有高热证候的气分证家兔模型,并用白虎汤和自拟方芩连液分别进行治疗,通过ELISA检测比较治疗前后血清总补体(CH50)的含量变化,并采用实时荧光定量PCR技术检测了肝组织中补体第三成分(C3)基因和C-反应蛋白(CRP)基因的表达变化。结果表明,在气分证家兔模型中,补体系统以经典途径活化。白虎汤的治疗能同时保证C3基因和CRP基因的高表达起到治愈作用,而芩连液仅以提高CRP的表达量发挥一定的保护作用。研究表明白虎汤对动物气分证的疗效优于芩连液。  相似文献   

Generalized Algebraic Difference Site Index Model for Ponderosa Pine in British Columbia,Canada     

Zhao Lei  Ni Chengcai  Gordon Nigh 《中国林业科技(英文版)》2012,(3):61-62

Based upon 3 widely used base models, a total of 8 ADA/GADA site index models were derived.The data for these models in this study were obtained from 79 pith-split stem analysis plots and the estimation method was "indicator variable approach".We used both fit statistics and visual analysis to select the best-fit model,and attached more importance to the visual analysis.A comprehensive application analysis was also given to the selected model.The results showed:1) GADA outperformed ADA with respect to predictions.2) A GADA model derived from HossfeldⅣpresented the best prediction ability.It was suggested that the model be used to predict dominant height and to estimate site index for ponderosa pine stands ranging 30 -200 years in British Columbia,Canada.3) The best site index age was age of 100 years,based upon relative errors of predictions.  相似文献   

A Serological Investigation into Contagious Caprine Pleuropneumonia (CCPP) in Ethiopia   总被引：4，自引：0，他引：4  

Sharew AD  Staak C  Thiaucourt F  Roger F 《Tropical animal health and production》2005,37(1):11-19

For a comparison of serological tests for CCPP, sera from 767 goats were examined. They were subjected to three tests: complement fixation test (CFT) with Mycoplasma capricolum subspecies capripneumoniae antigen; blocking ELISA (B-ELISA) with Mycoplasma capricolum subspecies capripneumoniae antigen; and CFT with Mycoplasma mycoides subspecies mycoides small colony type antigen. Antibodies were detected by these three tests in 23%, 2% and 12%, respectively, of sera from districts in which CCPP had not been reported, and in 60%, 83% and 87%, respectively, in sera from areas in which CCPP had been reported. The specificity of the tests is discussed. The use of the B-ELISA test for the diagnosis and for epidemiological studies of CCPP is strongly recommended.  相似文献   

Haemolytic Complement Activity and Humoral Immune Responses to Sheep Red Blood Cells in Indigenous Chickens and in Eight German Dahlem Red Chicken Lines with Different Combinations of Major Genes (dwarf,naked neck and frizzled) of Tropical Interest     

Baelmans R  Parmentier HK  Nieuwland MG  Dorny P  Demey F  Berkvens D 《Tropical animal health and production》2005,37(3):173-186

A total of 376 chickens from different ecotypes were immunized with the non-pathogenic multi-determinant antigen sheep red blood cells (SRBC). The ecotypes included indigenous chickens from various locations in Tanzania (n=102), India (n=86) and Bolivia (n=89). In addition, eight German Dahlem Red (GDR) chicken lines with different major genes (dwarf, naked neck and frizzled) of tropical interest were also immunized with SRBC. Immune competence of the breeds was assessed by measuring complement haemolytic activity, both from the classical calcium-dependent complement pathway (CPW) and alternative calcium-independent complement pathway (APW), alongside IgTotal, IgG and IgM antibody responses to SRBC at 7 days post immunization. Large variations in complement activity and antibody responses to SRBC were observed within and between the indigenous breeds. Many indigenous chickens, especially from Bolivia, showed decreased complement activity (APW) following immunization with SRBC. Breeds from India showed the highest CPW activity and humoral (especially IgM) responses to SRBC, suggesting high immune competence. In contrast, Bolivian chickens were characterized by low CPW activity, low APW activity and low antibody levels to SRBC suggesting an overall low immune competence. In the GDR chickens, characterized by high CPW activity and high IgG antibody responses to SRBC, the major genes for naked neck, frizzling and dwarfism had no significant effect on the antibody responses and complement activity to SRBC.  相似文献   

Demonstration of Alternative and Classical Complement Pathway Activity in Colostrum from Buffalo (Bubalus bubalis)     

Matheswaran K  Dhinakar Raj G  Nachimuthu K 《Veterinary research communications》2003,27(6):445-452

Buffalo colostrum caused lysis of unsensitized red blood cells (RBC) from sheep, goats, rabbits and chickens. RBC from cattle and buffalo were resistant to lysis. That lysis was due to the presence of natural antibodies to these RBC was ruled out since there was no reduction in haemolytic titres even after adsorption with the respective RBC. The addition of EGTA to the diluent had no effect on the haemolytic activity. These findings indicate the presence of alternative complement pathway (ACP) activity in buffalo colostrum. The haemolytic activity of buffalo complement for unsensitized rabbit RBC was reduced to very low levels by heating at 50°C for 45 min. Treatment with zymosan also inhibited the haemolytic activity, while inulin had no effect. The maximum activity of ACP occurred in the presence of 4 mmol/L Mg²⁺ in the diluent. The range of ACP activities in colostrum from buffaloes varied from 4.06 to 8.48 CH₅₀ units/ml. Using a standard system for titrating the classical complement pathway and rabbit red blood cells sensitized with goat haemolysin, the range of complement activity in buffalo colostrum was 4.81–6.77 CH₅₀/ml.  相似文献   

并行多值混合方法在一类2-指标微分代数方程中的应用     

李鑫  高威 《中南林业科技大学学报(自然科学版)》2007,27(3):163-166

将并行多值混合方法应用于一类2-指标微分代数方程的数值处理,并进行误差估计,获得了十分理想的结果.由于微分代数方程广泛应用于电网络分析、航空、航海、分工系统、动力、生态系统、化学动力学、最优控制、计算机辅助设计与建模以及社会学和经济学等各个领域,因此,研究微分代数方程的数值方法理论及应用具有现实意义和实用价值.  相似文献