尾叶桉U6遗传转化再生体系的建立   总被引：1，自引：0，他引：1  

孔华  郭安平  郭运玲  刘恩平  贺立卡 《热带作物学报》2008,29(4):424-429

以桉树品种尾叶桉U6叶盘为外植体,选用MS培养基为基本培养基,附加激素6-BA,IAA,IBA,NAAA,通过研究不同激素浓度组合对外植体愈伤诱导及不定芽分化的影响,建立了较好的遗传转化再生体系.结果表明,尾叶桉U6叶盘最适分化培养基为MS 6-BA 2.0 mg/L 2,4-D 1 mg/L IAA 0.2 mg/L 蔗糖30 g/L 琼脂粉5 g/L;小苗的最佳生根培养基为MS NAA 0.2 mg/L mA 0.5 m/L 蔗糖30 g/L 琼脂粉5 g,L.40mg/L卡那霉素可以抑制叶盘的分化;20 mg/L卡那霉素可以抑制再生植株的生根.2种抑菌抗生素中,头孢霉素对叶片再生影响较大;而250 mg/L的羧苄青霉素能有效地抑制农杆菌菌株EHA105的生长,却对尾叶桉叶盘的芽分化影响不大,为适宜的抑菌抗生素.3种农杆菌LBA4404,EHA105,GV3101的菌液浸染桉树叶盘,统计其愈伤组织GUS染色率,以EHA105对外植体的浸染能力最强,达83.3%.  相似文献   

Studies on browning problem and phenols content on shoots of Yali, Aikansui and Abbe Fetel pears for in vitro culture   总被引：1，自引：0，他引：1  

Bharat Kumar Poudyal  Guoqiang Du  Yuxing Zhang  Jie Liu  Qingchun Shi 《Frontiers of Agriculture in China》2008,2(3):321-330

Yali is one of the best pear cultivars cultivated extensively in China and other countries. However, mortality rates of explants during the initial phase of tissue culture were found to be very high during the summer particularly from the shoot tip explants. A thorough investigation on the browning problem of Yali Aikansui and Abbe Fetel pears was done and their control measures are suggested in this paper. Emphasis has been given to the Yali pear. Shoot explants were collected from the field as well as from the plantlets grown in vitro during different months and observed for browning. The explants were excised and cultured in Murashige and Skoog (MS) solid and liquid medium as needed. The cultures were maintained in the dark as well as in a 16/8 hours light/dark photoperiod regime as needed and were kept in a growth room at (25±2)°C temperature. According to the experimental results, Yali was found more severely infected by browning than the other two cultivars. Similarly, it was also found that, the intensity of browning was less during spring and increased with time and reached the maximum during summer. Shoot tips of Yali were found more severely infected by browning than the second node and other nodes. This accelerated the mortality rate up to 81% of shoot explants during the summer months of July and August. Browning was greatly influenced by water-soluble polyphenols, more of which were found in the shoot tip of explants collected from the field (significantly higher at P ⩽ 0.05 and P ⩽ 0.01). Total polyphenol contents were found less in the explants from plantlets grown in vitro, and less browning of medium appeared resulting in a lower mortality rate of explants. Collections of explants during early spring (P ⩽ 0.05 and P ⩽ 0.01) and use of other nodes rather shoot tip because explants (P ⩽ 0.05 and P ⩽ 0.01) were found to be better in preventing the browning problem. Finally, for curative measures to control browning, use of ascorbic acid at the rate of 100 mg·L⁻¹ (P ⩽ 0.05 and P ⩽ 0.01), 0.02%polyvinyl pyrrolidone (PVP) in the culture medium (P ⩽ 0.05), 96 hours dark treatment of other nodes (P ⩽ 0.05), and 12 hours cold treatments of explants at 4°C (P ⩽ 0.05 and P ⩽ 0.01) prior to sterilization of explants, were found to be the best methods to control browning and therefore to increase the survival rate of cultured explants of the Yali pear.  相似文献   

玉米不同外植体愈伤组织的诱导及植株再生的研究   总被引：8，自引：0，他引：8  

王昌涛  杨爱国  高树仁  赵琦  赵玉锦  张世煌 《沈阳农业大学学报》2005,36(5):515-518

以玉米自交系18红和18白为材料，取其幼胚、茎尖、成熟胚和下胚轴为外植体，对愈伤组织的诱导和植株再生进行了研究。结果表明：上述4种外植体均可诱导出愈伤，但仅从幼胚和茎尖诱导出胚性愈伤组织．转入分化培养基后获得了再生植株，而成熟胚和下胚轴不能获得再生植株。  相似文献   

北海道黄杨下胚轴的离体培养及植株再生   总被引：7，自引：0，他引：7  

尚爱芹  蔡汉  闰晓洁  胡海姿  赵梁军 《中国农业科学》2005,38(12):2502-2507

 以北海道黄杨(Euonymus japonicus 'Cu zhi')下胚轴为外植体进行离体培养，以MS和B5为基本培养基，附加不同浓度的细胞分裂素（6-BA，KT）及生长素（NAA，IBA）诱导下胚轴直接再生不定芽。结果表明，不定芽未经过愈伤组织而直接产生于下胚轴的表皮或近表皮等表层细胞；不同的激素浓度和组合以及不同的培养基对不定芽的分化有影响；下胚轴的不同部位不定芽的分化能力差异显著。适宜不定芽分化的最佳培养体系为MS+1.5 mg·L-16-BA+0.05 mg·L-1NAA，最佳外植体为靠近子叶端的下胚轴部分，其分化率最高达63.64%；不定芽增殖培养基为MS+2.0 mg·L-16-BA+0.2 mg·L-1NAA，增殖系数为3～5；1/2MS+1.0 mg·L-1IBA+100 mg·L-1活性炭适于再生幼苗的生根，生根苗经移栽成活。  相似文献   

油茶不同外植体灭菌条件研究   总被引：2，自引：0，他引：2  

刘海龙  陈晓明  蔡玲  吴幼媚  覃子海  杨开太 《广西林业科学》2010,39(2):61-63,68

以油茶种子、茎段为外植体,采用酒精、升汞、高锰酸钾3种灭菌剂,探讨不同处理对外植体污染和种子萌发的影响。结果表明,油茶茎段较好的灭菌方法:毛刷刷洗+75%酒精10~15 s+0.1%HgCl 10~15min,污染率最低为24.5%;新萌条较好的灭菌方法:75%酒精4~7 s+0.1%HgCl 5~6 min,污染率最低为14.3%;种子灭菌的条件:0.5%KMnO40~24 h+75%酒精3~15 min+0.1%HgCl 20~40 min(不剥壳去皮),75%酒精30 s+0.1%HgCl 8 min(剥壳去皮),这2种处理污染率均较低,种子发芽率相差不大。该结果为油茶再生体系的建立奠定基础。  相似文献   

Oxytocin Stimulated PGF₂α Release from Equine Uterine Explant Cultures to a Greater Extent than Isolated Uterine Epithelial Cell Cultures     

L. PenrodS.W. Limesand  M.J. Arns 《Journal of Equine Veterinary Science》2009

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不同时期及部位的外植体对香石竹芽形成的影响     

苏江  岑忠用  路艺聪 《北方园艺》2012,(2):129-131

在春、冬季取香石竹的顶芽、茎段芽进行离体培养,研究不同采集季节及部位的外植体对香石竹芽形成的影响。结果表明:在春季取顶芽做外植体采用70%酒精30s+0.1%升汞6min消毒处理可获得较好的试管苗。70%酒精30s+0.1%升汞6min较适宜于外植体消毒,在春季取材,顶芽分化优于茎段芽,在冬季取材,茎段芽分化优于顶芽。  相似文献   

茄子高频再生体系的建立与优化     

李烨  金丹丹  谢立波  李景富 《黑龙江农业科学》2014,(9):48-52

为建立并优化茄子高频再生体系,研究以茄子品种哈农杂茄2号和哈农杂茄3号为材料,利用不同的外植体和植物生长调节剂组合,探讨影响茄子再生频率的主要因素。结果表明:茄子最佳再生子叶最适分化培养基为MS 0+6-BA 1mg·L-1+KT 2mg·L-1;下胚轴最适分化培养基为MS 0+KT 2mg·L-1,再生苗最佳生根培养基为1/2MS+IAA 0.1mg·L-1,且下胚轴的分化效率高于子叶。  相似文献   

金合欢属植物组织培养研究进展   总被引：3，自引：1，他引：2  

唐佳佳  洑香香  尚旭岚  王纪 《世界林业研究》2014,27(4):30-35

金合欢属植物生长速度快、产量高、材质好、适应性强、用途广,在我国南方林业生产中占据重要地位。掌握该属植物的组织培养技术,对促进其产业发展具有重大意义。文中从外植体、基本培养基、培养条件、植物生长调节剂、组培苗移栽等方面简述20世纪80年代以来金合欢属植物组织培养技术的研究进展,并就组培快繁中存在的主要问题提出相应的解决方法,同时提出今后的研究展望,以期为建立金合欢属植物高效组培体系及工厂化育苗提供参考。  相似文献   

Comparative Propagation of shape Newcastle disease virus (Strains I-2 and V4) on Chicken Embryo Tracheal Explants     

Wambura PN 《Veterinary research communications》2006,30(6):673-677

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