传染性法氏囊病病毒变异株的致病性   总被引：6，自引：1，他引：5  

刘爵  刘有昌 《中国兽医学报》1998,18(5):427-429

传染性法氏囊病病毒变异ＧＣ９０２株可引起１０日龄ＳＰＦ鸡胚肝脏坏死和脾脏明显肿大及较高的死亡率。接种２周龄ＳＰＦ雏鸡,同时设标准Ｉ型强毒ＣＪ８０１株和标准变异株强毒１０８４Ａ株接种对照,该毒株接种后第３天才出现法氏囊粘膜浆膜出血、个别黄化、质硬等病变,且于第４天法氏囊明显萎缩变小,至接种后２０天法氏囊仍严重萎缩;接种后第２天引起脾脏显著肿大,于第１２天时大小恢复正常。试验结果表明,该ＧＣ９０２株对ＳＰＦ雏鸡的致病性与标准变异株基本一致,仅有一定的差异,但明显不同于标准Ｉ型强毒株的致病性。  相似文献   

传染性法氏囊病病毒的生态学与流行病学研究   总被引：18，自引：3，他引：15  

周宗安  王永山 《中国兽医学报》1998,18(5):430-433

血清学调查结果表明,麻雀、鸭、鹅等均可自然感染传染性法氏囊病病毒（ＩＢＤＶ）,其血清阳性率分别为７．４％（４／５４）,９５．５％（３６３／３８０）和９．４％（１１／１１７）。从鸭和麻雀分离到的ＩＢＤＶ可适应于鸡胚和鸡胚成纤维细胞,并产生ＣＰＥ,对ＳＰＦ鸡有致病性。病毒核酸和结构蛋白的电泳分析结果表明,鸡源、鸭源和麻雀源ＩＢＤＶ均有２条核酸带,５条蛋白带,毒株间的病毒核酸及结构蛋白电泳迁移率无显著差异,但其结构蛋白的相对含量不尽一致。经鉴定,鸡源、鸭源和麻雀源ＩＢＤＶ均属血清Ⅰ型,为同源病毒。本研究结果提示,鸡并非是ＩＢＤＶ的唯一自然动物宿主,非鸡禽鸟类宿主的存在可引起ＩＢＤ的传播和续源流行,也为ＩＢＤＶ的变异提供了特殊的生态条件,成为诱导ＩＢＤＶ变异的另一重要因素。  相似文献   

泰国红色原鸡和中国红色原鸡mtDNA控制区遗传多态性和分类关系研究     

吉挺  包文斌  束婧婷  栾德琴  Steffen weigend  陈国宏 《中国畜牧杂志》2008,44(23)

对泰国红色原鸡Gallus gallus gallus亚种和中国红色原鸡Gallus gallus spadiceus亚种各16个个体mtDNAD-loop序列进行系统分析,测定线粒体D-loop部分序列大小约为560bp,A、C、G、T这4种核苷酸的平均比例分别为13.6%、43.2%、4.3%和38.9%,A+T含量高于G+C含量。结果共发现27个变异位点,颠换和转换之比为0.13,没有观测到插入/缺失情况。测定的6种单倍型中,2个红色原鸡亚种没有共享单倍型,单倍型多样度分别为0.250和0.695,平均核苷酸差异数分别为3.750和10.833,核苷酸多样度分别为0.954%和2.757%。泰国红色原鸡中性检验的Tajima'sD值为-1.800(P<0.05),不符合中性突变。2个红色原鸡亚种间核苷酸分歧度(Dxy)为2.847%,核苷酸净遗传距离(Da)为0.991%。序列群体间的方差组分(Va)占总变异的47.31%,Fst=0.473,差异极显著(P<0.01);群体间mtDNAD-loopFst值也差异显著(P=0.035)。红色原鸡2个亚种具有不同的群体遗传结构,群体之间存在明显的遗传分化,本研究支持这2个亚种并非是同一个亚种的观点。  相似文献   

某养鸡场禽霍乱分离菌对常用抗菌药物的敏感性研究     

徐海军  周科伟 《家畜生态》2006,(3)

从六安地区某养鸡场禽霍乱病鸡上分离到1株禽多杀性巴氏杆菌,采用纸片扩散法和试管稀释法分别测定了青霉素钾、土霉素、硫酸卡那霉素、硫酸庆大霉素、硫酸链霉素、复方新诺明、甲砜霉素、氟哌酸、环丙沙星和恩诺沙星等10种药物对分离菌的体外抗菌活性。结果表明分离到的禽多杀性巴氏杆菌对青霉素钾和土霉素耐药;对硫酸卡那霉素、硫酸庆大霉素、硫酸链霉素、甲砜霉素、氟哌酸敏感;对复方新诺明、环丙沙星和恩诺沙星极敏感。两种测定方法得到的结果一致。因此复方新诺敏、环丙沙星和恩诺沙星可作为目前该发病场防治禽霍乱的首选药物。  相似文献   

The Hydropericardium Syndrome in Poultry – A Current Scenario   总被引：2，自引：0，他引：2  

Balamurugan V  Kataria JM 《Veterinary research communications》2004,28(2):127-148

Inclusion-body hepatitis hydropericardium syndrome (IBH-HPS) is an important, recently emerged, disease of poultry, particularly of 3- to 6-week-old broiler chicks, characterized by its sudden onset, with high mortality ranging from 20% to 70%, typical hydropericardium and enlarged mottled and friable livers, with intranuclear inclusion bodies in the hepatocytes. The causative agent is a non-enveloped icosahedral fowl adenovirus (FAV) serotype 4, belonging to the Adenovirus genus of the family Adenoviridae, which can be propagated or cultivated in chicken embryo liver and kidney primary cell cultures. The transmission of disease occurs vertically and laterally by the oral-faecal route. The liver of infected birds shows necrotic foci and basophilic intranuclear inclusion bodies in the hepatocytes. The disease can be diagnosed from its gross and histopathological changes in the liver and by various serological tests, such as agar gel immunodiffusion, counterimmunoelectrophoresis, indirect haemagglutination, the fluorescent antibody technique, enzyme-linked immunosorbent assay and the polymerase chain reaction. The disease has been brought under control by the use of formalin-inactivated vaccines, prepared from infected liver homogenate, and of inactivated cell culture vaccines. The vaccines are effective in the face of natural outbreaks or experimental challenge and significantly reduce the mortality.  相似文献   

Vaccinating chickens against avian influenza with fowlpox recombinants expressing the H7 haemagglutinin   总被引：10，自引：0，他引：10  

Boyle DB  Selleck P  Heine HG 《Australian veterinary journal》2000,78(1):44-48

OBJECTIVE: To evaluate the vaccine efficacy of a fowlpox virus recombinant expressing the H7 haemagglutinin of avian influenza virus in poultry. PROCEDURE: Specific-pathogen-free poultry were vaccinated with fowlpox recombinants expressing H7 or H1 haemagglutinins of influenza virus. Chickens were vaccinated at 2 or 7 days of age and challenged with virulent Australian avian influenza virus at 10 and 21 days later, respectively. Morbidity and mortality, body weight change and the development of immune responses to influenza haemagglutinin and nucleoprotein were recorded. RESULTS: Vaccination of poultry with fowlpox H7 avian influenza virus recombinants induced protective immune responses. All chickens vaccinated at 7 days of age and challenged 21 days later were protected from death. Few clinical signs of infection developed. In contrast, unvaccinated or chickens vaccinated with a non-recombinant fowlpox or a fowlpox expressing the H1 haemagglutinin of human influenza were highly susceptible to avian influenza. All those chickens died within 72 h of challenge. In younger chickens, vaccinated at 2 days of age and challenged 10 days later the protection was lower with 80% of chickens protected from death. Chickens surviving vaccination and challenge had high antibody responses to haemagglutinin and primary antibody responses to nucleoprotein suggesting that although vaccination protected substantially against disease it failed to completely prevent replication of the challenge avian influenza virus. CONCLUSION: Vaccination of chickens with fowlpox virus expressing the avian influenza H7 haemagglutinin provided good protection against experimental challenge with virulent avian influenza of H7 type. Although eradication will remain the method of first choice for control of avian influenza, in the circumstances of a continuing and widespread outbreak the availability of vaccines based upon fowlpox recombinants provides an additional method for disease control.  相似文献   

串联表达传染性喉气管炎病毒gB、gD抗原表位基因的重组鸡痘病毒转移载体的构建   总被引：1，自引：0，他引：1  

刘文波  梁成珠  徐彪  朱来华  张素芳  陈溥言 《吉林农业大学学报》2005,27(4):453-456,460

  相似文献   

鸡伤寒沙门菌的分离鉴定及药敏试验     

刘素英  王晶钰  董睿  王利勤  王慧 《动物医学进展》2011,32(9):37-41

为给鸡伤寒沙门菌病的防控提供理论依据与数据参考,采集疑似鸡伤寒病死鸡的肝、脾病料,进行细菌分离.根据细菌形态、培养特性、生化试验等鉴定出13株沙门菌.对分离到的菌株进行21种常用抗菌药物的敏感性试验,结果显示,分离株对阿米卡星、新霉素高度敏感,对卡那霉素、痢特灵中度敏感,而对其他药物则表现为耐药.将筛选出的阿米卡星对发...  相似文献   

免疫鸡群感染新城疫的诊断及其对策   总被引：1，自引：1，他引：1  

蔡家利  田茂春 《西南大学学报(自然科学版)》1992,14(5):457-459

本文报道免疫鸡群感染新城疫后,产蛋下降24%和HI 抗体水平上升4倍以上,并继发感染大肠杆菌而死亡。对此鸡群进行接种NDI 系苗表明,接种组产蛋率很快由56%恢复到70%,而对照组无恢复现象,这为免疫水平不高(抗体效价相似文献   

和缓艾美尔球虫的鉴定及致病性研究   总被引：1，自引：0，他引：1  

陶建平  符敖齐 《扬州大学学报(农业与生命科学版)》1996,(4)

采用单卵囊分离技术，从鸡球虫混合种中分离出一纯种卵囊，该卵囊呈球形或亚球形，囊壁光滑无色，无卵膜孔和极帽。卵囊内有１极体，无内、外残余体。测２００个卵囊的平均大小为（１４．６０±１．１２）μｍ×（１３．９６±１．０５）μｍ，测１００个孢子囊的平均大小为（７．８８±０．６４）μｍ×（４．７７±０．３９）μｍ。潜隐期为９５ｈ，最短孢子化时间为１５ｈ，虫体主要寄生于小肠下段。就以上指标对照文献，鉴定该种球虫为和缓艾美尔球虫（ＥｉｍｅｒｉａｍｉｔｉｓＴｙｚｚｅｒ，１９２９）。用此球虫卵囊１０万和５０万个分别感染１０日龄ＡＡ肉鸡各１０只，另设１０只为不感染对照组，结果感染组的平均增重与不感染对照纽的差异极显著（Ｐ＜０．０１），而肠道病变记分和死亡率均为零  相似文献