Internal fruit rot of netted melon caused by <Emphasis Type="Italic">Pantoea ananatis</Emphasis> (=<Emphasis Type="Italic">Erwinia ananas</Emphasis>) in Japan   总被引：1，自引：1，他引：0  

Kazutaka Kido  Rie Adachi  Masaru Hasegawa  Kazutaka Yano  Yasufumi Hikichi  Shigeharu Takeuchi  Tae Atsuchi  Yuichi Takikawa 《Journal of General Plant Pathology》2008,74(4):302-312

An internal fruit rot with a malodor was found in netted melons (Cucumis melo L.) in commercial greenhouses in Kochi Prefecture, Japan, in 1998, despite their healthy appearance and lack of water-soaking or brown spots on the surface. A yellow bacterium was consistently isolated from the affected fruits. To confirm the pathogenicity of eight representative isolates of the yellow bacterium, we stub-inoculated ovaries (immature-fruits) 5–7 days after artificial pollination, with a pin smeared with bacteria. After the melon fruits had grown for 60 more days, an internal fruit rot resembling the natural infection appeared, and the inoculated bacterium was reisolated. The melon isolates had properties identical with Pantoea ananatis, such as gram-negative staining, facultative anaerobic growth, indole production, phenylalanine deaminase absence, and acid production from melibiose, sorbitol, glycerol, and inositol. Phylogenetic analysis based on 16S rDNA sequences showed that the melon bacterium positioned closely with known P. ananatis strains. The melon bacterium had indole acetic acid (IAA) biosynthesis genes (iaaM and iaaH) and a cytokinin biosynthesis gene (etz). The bacterium could be distinguished from the other ‘Pantoea’ group strains by rep-PCR genomic fingerprinting. From these results, the causal agent of internal fruit rot was identified as a strain of P.ananatis [Serrano in (Philipp J Sci 36:271–305, 1928); Mergaert et al. in (Int J Syst Bacteriol 43:162–173, 1993)]. The nucleotide sequence data reported are available in the DDBJ database under accessions AB297969, AB373739, AB373740, AB373741, AB373742, AB373743 and AB373744.  相似文献   

Detection of Koi herpesvirus: impact of extraction method,primer set and DNA polymerase on the sensitivity of polymerase chain reaction examinations     

Kirsten Meyer  Sven Michael Bergmann  Marian van der Marel  Dieter Steinhagen 《Aquaculture Research》2012,43(6):835-842

Since virus isolation is seldom successful, KHV infection is commonly detected by PCR examination. A number of different PCR assays have been described in recent years. However, at present no commonly accepted PCR method is used amongst different laboratories. The aim of this study was to check if the examination of infected fish by different PCR methods yielded comparable results. We used tissue samples of three KHV‐infected koi, one KHV‐infected common carp, one KHV‐infected goldfish and one non‐infected common carp. DNA was extracted with DNAzol Reagent, High Pure PCR Template DNA Preparation Kit and QIAamp DNA Mini Kit. The DNA was tested by PCR with different combinations of published primer sets –KHV‐F and ‐R, KHV‐Gray‐2F and ‐2R and KHV‐TKf and ‐TKr – plus different DNA polymerases – a standard Taq DNA polymerase, a Platinum (hotstart) Taq DNA polymerase and a Platinum (hotstart) Pfx DNA polymerase with proofreading activity. The different extraction methods produced DNA solutions with different yields of DNA and different degrees of homogeneity. Also, the sensitivity of the PCR depended on the choice of the primer set and polymerase. Not all infected fish could be identified with all methods; there were large differences in the sensitivity between methods.  相似文献   

硫代硫酸钠法测定过氧化氢的最佳硫酸用量研究     

缪德仁  黄伟英  刘菲 《安徽农业科学》2011,39(30):18576-18578

[目的]采用Na2S2O3滴定法测定土壤液中H2O2含量经济、快捷,但酸度严重影响测定结果的准确性。[方法]对Na2S2O3法测定H2 O2时稀H2 SO4用量进行研究。[结果]对理论浓度为9.898 mol/L H2 O2样品进行6次平行测定,回收率达98.8%～100.8%,相对标准偏差（RSD）为0.83%,平均值为9.857 mol/L。[结论]只有稀H2SO4用量至少为理论值的2倍（[H＋]/[H2O2]≥4）,才能抑制H2O2歧化反应的发生,且Na2S2O3的歧化反应不会发生。  相似文献   

小麦Ta-hir2基因克隆及转Ta-hir2基因小麦的获得     

陈佳平  孟涛  刘芬  于秀梅  赵伟全  刘大群 《河北农业大学学报》2011,34(5):8-13

通过基因枪介导法将小麦过敏性诱导反应蛋白2基因Ta-hir2转入小麦胚性愈伤组织中,经分化筛选后获得转基因小麦植株,为验证该基因的功能奠定基础.以接种非亲和叶锈菌小种05-19-43②的小麦‘TcLr15,为材料,采用同源克隆的策略克隆了小麦Ta-hir2基因;将该基因的编码区与植物表达载体pAHC-25相连,成功构建...  相似文献   

基于SSR优化反应体系的茄子亲子鉴定     

王利英  乔军  石瑶  李素文 《山西农业科学》2011,39(8):782-785,790

利用分子标记SSR建立快速、准确可靠的纯度检测技术是控制杂交种纯度的有效措施。通过对茄子SSR反应体系进行优化,从40对备选引物中进一步筛选共显性多态标记,开展茄子的分子标记鉴定研究,试验结果显示,有7对SSR多态标记在2个杂交种及亲本中表现一致,且为共显性,条带清晰,可以作为茄子的亲子鉴定标记,便于明确父母本来源及杂交种纯度,提高育种效率。  相似文献   

濒危树种格氏栲ISSR-PCR反应体系的建立   总被引：3，自引：0，他引：3  

林义君  刘金福  潘东明  洪伟  吴则焰 《福建林学院学报》2011,31(2):115-119

以濒危珍稀树种格氏栲为材料,对ISSR反应体系中的各个主要影响因子进行优化筛选.首次建立可用于格氏栲ISSR-PCR分析的反应体系:20μL PCR反应体系中包括1ng·μL-1模板DNA,0.10 mmo1·L-1dNTP,2.0μL 10×PCR buffer (缓冲液),0.2μmol·L-1引物,0.037 5...  相似文献   

犬血型与安全输血研究进展     

丁晓麟  温海  贺星亮  张汇东 《江西农业学报》2011,23(1)

综述了犬血型系统、血型鉴定、交叉配血、供血犬选择以及输血反应等与安全输血相关的各方面的研究进展,系统探讨了临床上如何对犬进行安全、有效输血以及当前存在问题。  相似文献   

pH 升高对红壤硝化过程产生 N2O 的影响   总被引：2，自引：2，他引：0  

王小治  孙伟  尹微琴  封克 《土壤》2009,41(6):962-967

对红壤添加NaOH培养获得不同pH系列的土壤.通过室内培养试验.研究了3种pH条件下土壤的N_2O排放和无机N的变化情况.结果表明:硝化活性随土壤pH升高而增强:pH升高增加了土壤N_2O的释放;纯化学过程对N_2O散发的贡献随pH的升高而降低;Nitrapyrin在pH 4.8和pH 6.0时表现山硝化抑制作用,在pH 8.5时抑制效果不明显,且提高了培养期间pH8.5土壤N_2O的释放量.  相似文献   

不同还原糖与小麦水解蛋白Maillard反应的比较研究     

刘珊  周富臣  胡军 《安徽农业科学》2009,37(15):7001-7003

[目的]探讨还原糖种类对Maillard反应的影响。[方法]以小麦水解蛋白为混合氮源,分别与葡萄糖、果糖和木糖于相同条件下反应制备反应型香料,以铵态氮损失率为指标考察3种还原糖的Maillard反应活性,并对反应产物的醚溶性成分进行分析,同时对加入3种反应物的参比卷烟进行感官评价。[结果]3种还原糖与小麦水解蛋白的Maillard反应活性依次为木糖〉葡萄糖〉果糖;葡萄糖反应产物中主要含呋喃类化合物及2-乙酰吡咯,果糖反应产物中主要含取代吡嗪类化合物和呋喃甲醇,木糖反应产物中主要含呋喃类化合物等。[结论]果糖和木糖与小麦水解蛋白的反应产物可较好的改善参比卷烟的感官品质。  相似文献   

辣木RAPD最佳反应体系的构建     

李海渤  任安祥  马崇坚  王玉珍 《安徽农业科学》2009,(2):504-505

[目的]研究辣木（Moringa oleifera Lamarch．）PAPD最佳反应体系,为辣木RAPD扩增提供研究基础。[方法]以辣木为研究材料,通过正交设计建立辣木RAPD最佳反应体系。[结果]辣木最佳RAPD反应体系为：10×PCR Buffer 2．5μl,Taq酶0．5μl（2．0U／μl）,dNTPs4．0μl（2．0mmol／L）,primer 4．0μl（2．0μmol／L）,DNA2．0μl（30．0ng／μl）,ddH2O 12．0μl;反应总体积为25．0μl。[结论]RAPD可以应用于辣木研究。  相似文献