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北极狐出血性肠炎病原分离鉴定 总被引:1,自引:1,他引:0
从排血便为主要临床特征的濒死期育成北极狐肠内容物中分离到 5 株革兰氏阳性大杆菌,各株菌的 37℃ 8 小时厌气肉肝汤纯培养上清液 02m l小鼠尾静脉注射,12 小时内 100% 死亡。生理生化鉴定证实分离菌为产气荚膜杆菌( B.aerogenescapsulatus)。血清定型结果表明,分离的 5 株菌均为 A 型产气荚膜杆菌。以饲喂狐的变质鱼浸出液静脉注射小鼠引起死亡,并从该浸出液中也分离到 A 型魏氏梭菌,证实该病的发生是饲料传播。 相似文献
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《Communications in Soil Science and Plant Analysis》2012,43(10):1159-1177
The soil accumulation of metals and other elements as a result of human activities is a global concern. This investigation involves the use of commercially available extractions to estimate elemental abundance and availability. The need exists, especially in poor nations, to have cost-effective analytical capabilities to perform an initial screening of a soil resource to determine if a problem exists. Three extraction protocols are proposed: (i) an aqua regia digestion to estimate a baseline geochemistry, (ii) a pyrophosphate extraction to estimate elemental abundances associated with the soil organic fraction, and (iii) a water extraction to estimate the most available fraction. If the soil resource is impacted, then more refined and traditional methods are required to document the extent of impact for possible remediation. The three extraction protocols were applied to soils with little impact to illustrate the assessment potential for selected metals and p-block elements. 相似文献
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Twigs of 2–3-year-old Platanus occidentalis L. were used as experimental material to find the causes for the contamination and browning in the initial stages of tissue cultures. To compare the degree of browning of explants picked off from different growing seasons, the experimental material was excised from trees on each of the first ten days in January, March, May and July, 2006. The results indicated that the contamination and browning rates of the material cut off in January (14.2% and 30.6%, respectively) and March were somewhat lower than those in July. The pretreatment of soaking the explants in different anti-oxidants and absorbents at the same time could diminish some side effects. The pretreatment of using 10 g·L–1 vitamin C reduced the contamination and brown-ing rate effectively. An orthogonal experiment showed that the optimal factor and level arrangement is 0.5 mg·L–1 BA, 2.0 g·L–1 ac-tive carbon and 1.5 g·L–1 PVP which resulted in a browning rate of only 16.5%. In general, sampling period, physical properties and pretreatment of explants are the main factors responsible for the contamination and browning of material in the initial stages of P. occidentalis tissue cultures. 相似文献
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利用正交设计试验研究了3个不同种子预浸泡时间(6,12和24h),3种消毒剂(10%H2O2,30%NaClO和0.1%HgCl2)和3个不同消毒时间(10,20和30min)对甘肃红豆草种子萌发率、污染率及发芽指数的影响,以获取甘肃红豆草无菌苗时种子的最佳消毒方法。结果表明,3个因素对发芽率影响差异显著;预浸泡时间对发芽指数影响差异极显著,而对污染率影响差异不显著;消毒剂种类及消毒时间对污染率和发芽指数影响差异显著。综合各项因素,室温条件下无菌水预浸泡24h、0.1%HgCl2消毒20min种子发芽率较高,污染率较低,发芽较快且出苗整齐,是获取甘肃红豆草无菌苗种子的最佳消毒方式。 相似文献
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Scorza Júnior RP Jarvis NJ Boesten JJ van der Zee SE Roulier S 《Pest management science》2007,63(10):1011-1025
Testing of pesticide leaching models against comprehensive field-scale measurements is necessary to increase confidence in their predictive ability when used as regulatory tools. Version 5.1 of the MACRO model was tested against measurements of water flow and the behaviour of bromide, bentazone [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one-2,2-dioxide] and imidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine] in a cracked clay soil. In keeping with EU (FOCUS) procedures, the model was first calibrated against the measured moisture profiles and bromide concentrations in soil and in drain water. Uncalibrated pesticide simulations based on laboratory measurements of sorption and degradation were then compared with field data on the leaching of bentazone and imidacloprid. Calibrated parameter values indicated that a high degree of physical non-equilibrium (i.e. strong macropore flow) was necessary to describe solute transport in this soil. Comparison of measured and simulated bentazone concentration profiles revealed that the bulk of the bentazone movement in this soil was underestimated by MACRO. Nevertheless, the model simulated the dynamics of the bentazone breakthrough in drain water rather well and, in particular, accurately simulated the timing and the concentration level of the early bentazone breakthrough in drain water. The imidacloprid concentration profiles and its persistence in soil were simulated well. Moreover, the timing of the early imidacloprid breakthrough in the drain water was simulated well, although the simulated concentrations were about 2-3 times larger than measured. Deep groundwater concentrations for all substances were underestimated by MACRO, although it simulated concentrations in the shallow groundwater reasonably well. It is concluded that, in the context of ecotoxicological risk assessments for surface water, MACRO can give reasonably good simulations of pesticide concentrations in water draining from cracking clay soils, but that prior calibration against hydrologic and tracer data is desirable to reduce uncertainty and improve accuracy. 相似文献
39.
真菌毒素是由某些丝状真菌产生的致癌、致畸、致突变的毒性副产物,对玉米、水稻、小麦、花生、大豆和油菜等多种粮油作物具有极大的危害性,严重威胁环境安全、食品安全和人类健康。随着世界人口的急剧增加和主要农作物种植面积的扩大,粮油农产品中的真菌毒素污染有逐年加重的趋势。本文对粮油作物中主要真菌毒素种类及污染现状进行了综述,分析了导致真菌毒素污染加重的环境影响因子,提出了粮油作物不同生长时期真菌毒素污染防控措施,特别是生物防控技术,展望了真菌毒素污染生物防控的作用模式及类型。 相似文献
40.
湖南耕地镉铅等重金属污染比较严重,研究有效治理耕地重金属污染方式和替代产业调整模式,已成为湖南省农业可持续发展和生态系统安全的关键。该项实验在岳阳市某工矿区选择典型的镉铅超标农田栽桑养蚕,桑树生长发育良好。结果表明,通过镉铅超标农田养蚕的经济性状及效益比较,在工矿区镉铅超标农田以栽植农桑14号品种较好,密度为每667m2栽植1 000~2 000株;并证实了镉铅污染农田栽桑养蚕的可行性,为镉铅等重金属污染耕地安全、经济、高效生态利用和污染区产业结构调整进行了有益的探索。 相似文献