嗜水气单胞菌对三种鱼血红蛋白的影响     

张文香  蔡小辉  葛慕湘  靳晓敏  王志敏 《河北渔业》2009,(11):7-10

以嗜水气单胞菌感染鲤鱼、鲫鱼、乌鳢,并检测了第4d和第8d的血红蛋白含量。同时,观察感染后鱼所出现的症状。结果表明,鲤鱼、鲫鱼出现典型的由嗜水气单胞菌引起的败血症症状,乌鳢则为腐皮病症状;与对照组相比,三种感染鱼的血红蛋白含量在第4d都显著下降(P<0.05),到第8d均极显著降低(P<0.01)。  相似文献   

月鳢年龄与生长的研究↑（*）   总被引：4，自引：0，他引：4  

杨代勤  陈芳  方长琰  罗静波 《中国水产科学》1999,6(3):10-13

月鳢鳞片可作为年龄鉴定的依据,其年轮形成时期在4～6月。鳞长与体长呈直线关系,体长和体重呈幂函数关系。月鳢2龄以前生长较快,2龄以后生长逐渐减慢,其生长适合于VonBertalanffy生长方程。为有效保护月鳢资源,应限制捕捞体重在200g以下的个体。  相似文献   

Isolation and characterization of Aeromonas schubertii from diseased snakehead, Channa maculata (Lacepède)     

Chen YF  Liang RS  Zhuo XL  Wu XT  Zou JX 《Journal of fish diseases》2012,35(6):421-430

Pure bacterial cultures were isolated from diseased snakeheads, Channa maculata (Lacepède), suffering high mortality in a farm in Zhongshan, southern China. Three isolates, namely ZS20100725, ZS20100725-1 and ZS20100725-2, were identified as Aeromonas schubertii. All the isolates showed high 16S rRNA sequence similarities with A. schubertii. The isolates exhibited strong virulence to snakeheads in experimental challenges with LD(50) ranging between 1.4 × 10(4) and 6.4 × 10(6) CFU g(-1). Two of the isolates were positive for haemolysin, elastase, lipase and lecithinase by phenotypic determination, which was further confirmed by PCR amplification of the haemolysin and elastase genes. In sterile liquid medium, the best growth conditions of strain ZS20100725 were 30 °C, pH 7 and 0.5% salinity (w/v). Antibiotic susceptibility tests showed that strain ZS20100725 was susceptible to cefoxitin, cefoperazone and chloramphenicol. Furthermore, histopathology of diseased snakeheads infected with A. schubertii showed necrosis and congestion in liver, kidney and spleen and also damage to the cardiac muscle, intestine and gills.  相似文献   

Effect of lipid levels on the reproductive performance of Snakehead murrel,Channa striatus          下载免费PDF全文

Alireza Ghaedi  Muhammad Anamul Kabir  Roshada Hashim 《Aquaculture Research》2016,47(3):983-991

Present study aimed to determine the optimum dietary lipid level in snakehead murrel channa striatus broodstocks. Triplicate groups of fish were fed for 240 days with isonitrogenous experimental diets with increasing lipid levels (100, 140, and 180 g kg^?1), using fish oil and soybean oil as the lipid sources with the ratio of (1:1). Weight gain, GSI, fecundity, oocyte diameter and number of mature oocyte were found to be significantly higher in the group fed with diet containing 180 g kg^?1 lipid level. Muscle fatty acid profile showed a significant increase in LA (18:2n‐6), LNA (18:3n3), total PUFA, n‐6 and ArA (20:4n‐6) in fish fed with diet containing 180 g kg^?1 lipid. Increasing lipid level up to 180 g kg^?1 resulted in significant increase in PUFA (LA & LNA), lc‐PUFA (EPA, DHA, ArA), total PUFA, n‐3 and n‐6 series in ovary and liver of female C. striatus.  相似文献   

Induced Spawning of Striped Snakehead,Channa striatus,Using Ovatide     

《Journal Of Applied Aquaculture》2013,25(4):95-103

ABSTRACT

An experiment was investigated to evaluate the effects of different doses of a new spawning agent Ovatide on induced spawning of striped snakehead, Channa striatus. Nine matured females with swollen abdomen and 18 male fishes with body weights ranging from 750 to 900 g, were randomly assigned to three treatment groups. Fishes in each group were administered 0.2, 0.4, and 0.6 mL of Ovatide/kg body weight, respectively. Each breeding set consists of two males and one female. Partial spawning response was observed when the fishes were administered with 0.2 mL/kg BW of Ovatide. Complete spawning was noticed in the medium (0.4 mL/kg BW) and higher (0.6 mL/kg BW) dose administered groups. The latency period and number of eggs spawned ranged from 23 to 27 hours and 1,764 to 7,096, respectively. No significant difference (P > 0.05) was noticed in the fertilization and hatching rate between the medium and higher doses of Ovatide tested. Thus, C. striatus could be successfully induced to spawn using a synthetic gonadotropin-releasing hormone with a dopamine antagonist at the dose of 0.4 mL/kg BW under captive conditions at 29 ± 1.5°C.  相似文献   

鰤鱼诺卡氏菌对乌鳢血液指标的影响     

孙东雨  沈理  金珊  缪燕萍  赵青松  陈寅儿 《广西农业生物科学》2012,(3):289-294

应用浓度为106 cfu/mL的鰤鱼诺卡氏菌（Nocardia seriolea）菌悬液腹腔注射乌鳢,人工感染诺卡氏菌病.在感染后3 d、6 d、9 d、12 d、18 d和24 d时抽取乌鳢血液,检测乌鳢的血细胞数、血细胞脆性、溶菌酶、血清总蛋白、碱性磷酸酶、乳酸脱氢酶和尿素氮等指标.结果表明,与对照组相比,感染鰤鱼诺卡氏菌后乌鳢血细胞数呈先升高后降低的趋势,而血细胞脆性在各实验时间点均高于对照组;血清碱性磷酸酶呈先降低后升高趋势,血清溶菌酶、血清总蛋白和血清尿素氮等均呈先降低后增高再降低的趋势,血清乳酸脱氢酶活力显著增加.说明鰤鱼诺卡氏菌感染会引起乌鳢血液指标明显变化.  相似文献   

土池和高位铺膜池养殖杂交鳢肠道菌群结构的比较分析     

胡世康  武海波  王博  秦海鹏  廖栩峥  赵吉臣  何子豪  陈燮燕  孙成波 《大连海洋大学学报》2021,36(2):303-309

为研究土池和高位铺膜池两种养殖模式下杂交鳢Channa maculata♀×Channa maculata argus♂肠道菌群结构的差异,采用高通量测序方法分别对土池养殖的健康杂交鳢(体质量为1140.67 g±45.88 g)和高位铺膜池养殖的健康杂交鳢(体质量为1130.00 g±96.27 g)肠道菌群结构进行了对比分析,其中,土池设置3个重复组,分别记为abce123、abce124和abce125,高位铺膜池设置3个重复组,分别记为abce126、abce127和abce128。结果表明:高位铺膜池的水温、pH与土池无显著性差异,但总氨氮、亚硝酸氮和总碱度等明显低于土池;不同养殖模式下杂交鳢肠道菌群结构存在明显差异,且土池养殖杂交鳢肠道中细菌多样性远高于高位铺膜池;在门水平上,土池杂交鳢肠道样品abce123中变形菌门所占比例明显高于其他门类,样品abce124、abce125中分别以变形菌门Proteobacteria和拟杆菌门Bacteroidetes所占比例最高,而高位铺膜池3个肠道样品中均以梭杆菌门Fusobacteria所占比例最高;在属水平上,土池abce123肠道样品中优势细菌属类均为未知细菌属类,abce124肠道样品中优势细菌属类按丰度依次为金黄杆菌属Chryseobacterium、支原体属Mycoplasma、食酸菌属Acidovorax、不动杆菌属Acinetobacter和鲸杆菌属Cetobacterium,abce125肠道样品中优势细菌属类按丰度依次为金黄杆菌属、不动杆菌属、食酸菌属、支原体属和鲸杆菌属,而高位铺膜池3个样品中均以鲸杆菌属为主,占95%以上。研究表明,土池和高位铺膜池两种不同养殖模式下杂交鳢肠道微生物组成及多样性差异较大,其结果可为今后杂交鳢的养殖模式选择和微生态学研究提供数据参考。  相似文献   

条沙叶蝉生物学与生态学研究   总被引：2，自引：0，他引：2  

相建业  朱象三  刘绍友 《植物保护学报》1996,23(4):327-332

作者依据成虫产卵、若虫存活与发育等指标测定了条沙叶蝉对19种供试禾本科作物及杂草的寄主适应性,明确寄主植物14种,食料植物4种,非寄主食料植物1种。同时发现,不同小麦品种对条沙叶蝉存在不同程度抗虫性。通过室内试验和田间调查,明确条沙叶蝉各虫态生活习性及在关中年生活史和发生特点,在关中,条沙叶蝉1年发生4代,以卵越冬,全年各世代主要在杂草地完成周年发育循环,麦田发生条沙叶蝉主要是在小麦秋苗期。在20、25、30、35℃4种恒温与狗尾草、小麦、玉米3种食料条件下初步明确温度、食料对条沙叶蝉若虫发育、存活与成虫寿命及产卵的影响。  相似文献   

Biochemical alteration induced by monocrotophos in the blood plasma of fish, Channa punctatus (Bloch)     

Shweta Agrahari  Krishna Gopal 《Pesticide biochemistry and physiology》2007,88(3):268-272

Monocrotophos (MCP), commonly known as azodrin, is one of the organophosphate (OP) pesticides extensively used in agricultural practices throughout the world. Channa punctatus were exposed to sublethal concentrations (0.96 and 1.86 mg/L) of monocrotophos for 15 and 60 days to assess the alterations in the level of some biochemical parameters in blood plasma. Significant alterations in all the biochemical parameters were found to be dose dependent. Hypoglycemia and hypocholesteremia were observed in plasma of fish at both exposure periods (15 and 60 days). Increased activities of glutamate-oxalacetate transaminase (GOT), glutamate-pyruvate transaminase (GPT), acid and alkaline phosphatase of blood plasma indicated hepatic tissue damage. Decrease in lactate dehydrogenase (LDH) content in plasma further indicated lower metabolic rate after 60 days of exposure. Significant decline in triglycerides content was observed in fish exposed to both sublethal concentrations of monocrotophos. We suggest that analysis of biochemical parameters in the fish blood may be useful in environmental biomonitoring.  相似文献   

Inhibition of Na-K-ATPase in different tissues of freshwater fish Channa punctatus (Bloch) exposed to monocrotophos     

Shweta Agrahari 《Pesticide biochemistry and physiology》2008,92(2):57-60

Freshwater fish, Channa punctatus, commonly known as the snakehead fish, was exposed to two sublethal concentrations (0.96 and 1.86 mg/L) (selected on the basis of 1/20 and 1/10 of 96 h LC₅₀ value) of monocrotophos for two exposure periods (15 and 60 days). Effects of monocrotophos on Na⁺, K⁺-ATPase in liver, kidney, muscle, intestine, brain, heart and gills were determined. Results indicate that Na⁺, K⁺-ATPase activity in tissues decreased as concentration of monocrotophos and exposure period increased. Monocrotophos induced significant inhibitory effects on the Na⁺, K⁺-ATPase activity of C. punctatus, ranging from gills (70%) > Kidney (63%) > brain (57%) > intestine (52%) > liver (50%) > muscle (47%) > heart (44%) inhibition at a sublethal concentration of 0.96 mg/L. Significant inhibition was detected in Na⁺, K⁺-ATPase activity, ranging from gills (90%) > heart (78%) > kidney (78%) > muscle (74%) > intestine (71%) > brain (67%) > liver (63%) at sublethal concentration of 1.86 mg/L. After subacute exposure (15 days) only gills and brain showed significant inhibition after higher concentration (1.86 mg/L). However, it is evident that exposure duration is more important than dose in the inhibition of the activity of enzyme. At lower concentration initial stimulation of the activity of Na⁺, K⁺-ATPase activity was also noticed. It is suggested that the inhibition of the ATPase by monocrotophos blocked the active transport system of the gill epithelial as well as chloride cells, glomerular and epithelial cells of the tubules and thus altered the osmoregulatory mechanism of the fish. In fact, the impairment of the activity of enzymes which carry out key physiological roles could cause alterations of the physiology of the whole organism.  相似文献