杜泊羊血液蛋白质多态性分析     

王建刚  盛熙辉  郁枫  曹斌云 《黑龙江畜牧兽医》2006,(4):15-18

采用垂直板高pH值不连续聚丙烯酰胺凝胶电泳对21只杜泊羊的血红蛋白(Hb)、转铁蛋白(Tf)、白蛋白(Alb)、前白蛋白(Pa)和慢α2球蛋白(Sα2)5个基因座的多态性进行了分析。结果表明杜泊羊的Hb、Tf位点存在多态性,Hb位点共检测到HbA和HbB2个等位基因,构成HbAA、HbBB、HbAB3种基因型,其中HbBB和HbB为优势基因型和优势基因;Tf位点共检测到TfDD、TfCC、TfBD、TfCD4种基因型,受TfB、TfC、TfD3个复等位基因控制,其中TfCC、TfDD基因型和TfC、TfD基因为优势基因型和优势基因。试验未在Alb、Pa和Sα2位点检测出多态性,它们均呈现单态。  相似文献   

3种雉科鸟类血液生理指标的比较研究   总被引：11，自引：0，他引：11  

周天林  韩芬茹 《经济动物学报》2006,10(2):85-87

为了解勺鸡、雉鸡和石鸡血液生理参数的正常值及其特点,为人工饲养繁殖以及健康状况监测提供参考依据,采用常规方法,对人工饲养的3种雉科鸟类(勺鸡、雉鸡和石鸡)红细胞数、白细胞总数、白细胞分类计数、血小板数、血红蛋白含量、红细胞沉降率、红细胞体积及红细胞比容8项血液生理指标进行了测定。结果表明,雉鸡的血小板明显高于勺鸡和石鸡(P<0.05);其它7项血液生理指标3种鸟之间差异不显著(P>0.05)。  相似文献   

东北白鹅和籽鹅血液生化指标   总被引：5，自引：2，他引：5  

康波  杨焕民  刘胜军  周瑞进  李士泽  姜冬梅 《中国兽医学报》2006,26(6):649-652

为建立东北白鹅和籽鹅血液生化指标的正常参考值及其正常参考范围,探讨东北白鹅和籽鹅的各项血液生化指标之间的差异显著性,随机抽取(12±0.5)月龄健康、体质量为(4.2±0.2)kg的东北白鹅母鹅和(3.5±0.2)kg的籽鹅母鹅各65只,空腹12h后翅下静脉采血,离心分离血清,应用半自动生化分析仪及试剂盒对血清22项生化指标进行检测分析。结果表明:东北白鹅和籽鹅丙氨酸氨基转移酶(ALT/GPT)、天门冬氨酸氨基转移酶(AST/GOT)、谷氨酸酰基转移酶(GT)、胆碱脂酶(CHE)、乳酸脱氢酶(LDH-L)、葡萄糖(Glu)、高密度脂蛋白胆固醇(HDL-C)、总蛋白(TP)、白蛋白(Alb)、钙(Ca)、肌酐(Cre)、镁(Mg)等血清生化指标之间差异达到极显著水平(P<0.01);碱性磷酸酶(ALP)、总胆固醇(CHO)、无机磷(P)之间差异达到显著水平(P<0.05);a-羟丁酸脱氢酶(a-HBDH)、尿素氮(BuN)、甘油三脂(TG)、尿酸(UA)、总胆红素(T.Bili)、直接胆红素(D.Bili)、氯(Cl)之间无显著性差异(P>0.05)。本试验建立的东北白鹅和籽鹅血液生化指标的正常参考值及其正常参考范围,为今后鹅的相关研究提供了基础的科学数据和基本的理论依据。  相似文献   

In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood     

Ki-Soo Park  Yong-Soon Lee  Kyung-Sun Kang 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(4):343-348

Mesenchymal stem cells (MSCs) have the capabilities for self-renewal and differentiation into cells with the phenotypes of bone, cartilage, neurons and fat cells. These features of MSCs have attracted the attention of investigators for using MSCs for cell-based therapies to treat several human diseases. Because bone marrow-derived cells, which are a main source of MSCs, are not always acceptable due to a significant drop in their cell number and proliferative/differentiation capacity with age, human umbilical cord blood (UCB) cells are good substitutes for BMCs due to the immaturity of newborn cells. Although the isolation of hematopoietic stem cells from UCB has been well established, the isolation and characterization of MSCs from UCB still need to be established and evaluated. In this study, we isolated and characterized MSCs. UCB-derived mononuclear cells, which gave rise to adherent cells, exhibited either an osteoclast or a mesenchymal-like phenotype. The attached cells with mesenchymal phenotypes displayed fibroblast-like morphologies, and they expressed mesenchym-related antigens (SH2 and vimentin) and periodic acid Schiff activity. Also, UCB-derived MSCs were able to transdifferentiate into bone and 2 types of neuronal cells, in vitro. Therefore, it is suggested that the MSCs from UCB might be a good alternative to bone marrow cells for transplantation or cell therapy.  相似文献   

Effect of soluble glass bolus administration on the blood selenium concentration in grazing Philippine goats under backyard conditions     

Maki HAYASHIDA  Edgar A. ORDEN  Emilio M. CRUZ  Libertado C. CRUZ  Tsutomu FUJIHARA 《Animal Science Journal》2006,77(6):595-602

The present study was conducted to determine the effects of selenium (Se) soluble glass bolus (SGB) on blood Se concentrations of mixed‐bred (native × Anglo Nubian) pregnant does weighing between 15 and 52 kg and grazing on natural pastures in the Philippines. The control group, consisting of 28 does, was not administered with SGB, while 23 does were administered with SGB every 6 months for a period of 12 months. Forage and blood samples in both groups were collected every 2 months. Their Se, sulfur, calcium, phosphorus, magnesium, copper and zinc concentrations were analyzed using the wet digestion method. The results showed that the grazed forage had concentrations of all these minerals that were far higher than the accepted critical lower levels. The Se concentration was higher (P < 0.05) in the dry season than in the rainy season, especially in the whole blood collected from the does and newborn kids. In both groups the Se concentration in the kids tended to increase during the suckling period but decreased after weaning. Kids born to the SGB does had higher whole blood Se concentrations (P < 0.05) than those born to the control does. However, the administration of SGB to the does did not affect any blood mineral concentrations except for Se in the grazing does and their kids. The animals did not exhibit any clinical signs of mineral deficiencies or toxicities during the experimental period.  相似文献   

Evaluation of cardiac performance of the dog after induction of portal hypertension and gastric ischemia     

Catriona M. MacPhail  DVM  DACVS    Eric Monnet  DVM  PhD  DACVS  DECVS    Davyd H. Pelsue  DVM  DACVS    James S. Gaynor  DVM  MS  DACVA 《Journal of Veterinary Emergency and Critical Care》2006,16(3):192-198

Objective: To determine changes in hemodynamic and cardiac energetic parameters in dogs after induction of portal hypertension and gastric ischemia. These blood flow alterations are similar to changes seen in splanchnic blood flow in dogs with gastric dilatation volvulus syndrome (GDV). Design: Original experimental study. Setting: Veterinary teaching hospital. Animals: Seven purpose‐bred, intact male dogs. Interventions: Standard midline laparotomy and median sternotomy were performed under general anesthesia. Dogs were instrumented to obtain arterial blood pressure, aortic flow, cardiac chamber pressures, central venous pressure, portal flow, and portal pressure. Colored microsphere technology was used for the determination of myocardial blood flow. Measurements and samples were obtained at baseline, following induction of portal hypertension, and after induction of portal hypertension and gastric ischemia. Measurements and main results: Left ventricular myocardial blood flow was increased from 81.8±20.1 mL/100 g/min at baseline to 127.7±57.2 mL/100 g/min (P=0.02) after induction of portal hypertension and gastric ischemia. Myocardial oxygen consumption increased from 142.2±27.4 J/min/100 g at baseline to 219.1±33.4 J/min/100 g (P=0.003) after induction of portal hypertension and gastric ischemia, but cardiac external work remained unchanged (13.67±6.2 to 13.27±9.6 J/min; P=0.78; power=0.79). Cardiac efficiency decreased from 11.6±6.1% at baseline to 7.6±5.1% (P=0.017) after induction of portal hypertension and gastric ischemia. Conclusions: Transfer of energy within the myocardium was less efficient after induction of portal hypertension and ischemia of the stomach wall. On the basis of these results, alterations in cardiac function associated with GDV may result from deterioration of cardiac efficiency.  相似文献   

Biological characters of mesenchymal stem cells of human umbilical cord blood     

ZHOU Dun-hua  WEI Jing  HUANG Shao-liang  FANG Jian-pei  BAO Rong  WU Yan-feng  LI Yang 《园艺学报》2006,22(8):1606-1609

AIM：To study the isolation,expansion and purification of mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB),and investigate some biological identities of MSCs.METHODS：(1) MSCs of UCB,adult bone marrow (BM) and fetus BM were isolated by centrifugation with Ficoll,and the different kinds of MSCs were observed everyday.(2) Surface markers of MSCs were identified by flow cytometry.(3) The level of HGFs (TPO,SCF,FLT-3L,IL-6) secreted by different sources of MSCs was checked by ELISA method.RESULTS：(1) No difference in morphology of the colonies between UCB MSCs and BM MSCs was observed.However,the mononuclear cells needed in culture of UCB MSCs was about 3 times more than that in culture of BM MSCs.The times of UCB MSCs colony formation and confluencing were longer than that of BM in primary culture.(2) After passaged,there was no significant difference in the proliferation rates of 3 kinds of MSCs.Only 4 of 15 UCB samples contained a homogeneous population of MSCs.(3) UCB MSCs shared the same markers with BM MSCs.Neither hematopoietic marker nor immunologic recognition antigens were expressed.(4) The level of hematopoietic growth factors (HGFs) secreted by 3 kinds of MSCs was similar.CONCLUSIONS：(1) MSCs were isolated from UCB,but the amount of MSCs in UCB was smaller than that in BM,and just seldom samples of UCB contained homogeneous MSCs.(2) MSCs from UCB and BM shared the same biological characteristics,such as proliferation ability,surface markers,immunophenotypes and HGFs secretion.  相似文献   

Effect of different PRA serum levels from patients with β-thalassemia on the proliferation and differentiation potential of the hematopoietic stem cells /progenitor cells of cord blood     

WENG Wen-jun  FANG Jian-pei  WU Yan-feng  XIAO Lu-lu  XU Hong-gui  BAO Rong  WEI Jing  XU Lü-hong 《园艺学报》2007,23(11):2200-2204

AIM: To probe the effect of different panel reactive antibody(PRA) serum levels from patients with β-thalassemia on the proliferation and differentiation potential of the hematopoietic stem /progenitor cell of cord blood.METHODS: 1×105 mononuclear cells (MNCs) isolated from umbilical cord blood were incubated with different PRA serum levels (0 μL,50 μL,100 μL) respectively and complement,inoculated into the methylcellulose cultural system.The proliferation and differentiation potential of the hematopoietic stem /progenitor cell of cord blood by the colony formation assay were detected on day 7 and day 14,respectively.RESULTS: After culture of 7 days,the total colonies and CFU-GM were 88.20±9.41,79.00±11.39 in group A and 88.60±9.12,79.20±10.44 in group B,which were significantly higher than those of 20.60±7.39,15.20±4.66 in group C and those of 4.00±2.05,1.40±0.51 in group D (P<0.01).Meanwhile compared with group A and group E,no significant difference was found (P>0.05).After culture for 14 days,the total colonies and CFU-GM were 216.00±31.10,117.40±24.80 in group A and 213.20±31.06,116.00±19.75 in group B,which were significantly higher than those of 97.80±14.43,32.80±8.10 in group C and those of 31.40±13.41,8.40±4.30 in group D (P<0.01).The CFU-GEMMs were 45.60±8.51 in group A and 42.60±7.03 in group B,which were significantly higher than those of 20.80±6.96 in group C and those of 7.80±6.06 in group D (P<0.05).The BFU-MK was 12.80±4.42 in group A and 11.00±2.74 in group B respectively,which were significantly higher than that of 1.00±0.55 in group D (P<0.05).The CFU-E in group B 17.20±4.03 was significantly higher than that of 5.60±2.87 in group D (P<0.05).Meanwhile compared with group A and group E,no significant difference was found (P>0.05).By the Kendall test,there were negative correlations between the level of PRA serum and the total colonies,CFU-GM on day 7,the total colonies,CFU-GM,CFU-GEMM,BFU-E,BFU-MK on day 14 (tau-b=-0.793,-0.849,-0.808,-0.804,-0.645,-0.674,-0.624,P<0.01).There was a negative correlation between the level of PRA serum and CFU-MK on day 14 (tau-b=-0.466，P<0.05).CONCLUSION: PRA sera inhibit the colony in the colony cultures of the hematopoietic stem cells/progenitor cells in cord blood.The inhibition depends on the level of PRA sera.The higher the level of PRA sera,the stronger the inhibition is observed in our study.  相似文献   

Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop     

F. A. Zuckermann  M. D. Pescovitz  B. Aasted  J. Dominguez  I. Trebichavsky  B. Novikov  I. Valpotic  J. Nielsen  S. Arn  D. H. Sachs  J. K. Lunney  P. Boyd  J. Walker  R. Lee  W. C. Davis  I. R. Barbosa  A. Saalmü  ller 《Veterinary immunology and immunopathology》1998,60(3-4):291-303

Based on an analysis of their reactivity with porcine peripheral blood lymphocytes (PBL), only three of the 57 mAbs assigned to the T cell/activation marker group were grouped into cluster T9 along with the two wCD8 workshop standard mAbs 76-2-11 (CD8a) and 11/295/33 (CD8b). Their placement was verified through the use of two-color cytofluorometry which established that all three mAbs (STH101, #090; UCP1H12-2, #139; and PG164A, #051) bind exclusively to CD8⁺ cells. Moreover, like the CD8 standard mAbs, these three mAbs reacted with two proteins with a MW of 33 and 35 kDa from lymphocyte lysates and were, thus, given the wCD8 designation. Because the mAb STH101 inhibited the binding of mAb 76-2-11 but not of 11/295/33, it was given the wCD8a designation. The reactivity of the other two new mAbs in the T9 cluster with the various subsets of CD8⁺ lymphocytes were distinct from that of the other members in this cluster including the standards. Although the characteristic porcine CD8 staining pattern consisting of CD8^low and CD8^high cells was obtained with the mAb UCP1H12-2, a wider gap between the fluorescence intensity of the CD8^low and CD8^high lymphocytes was observed. In contrast, the mAb PG164A, not only exclusively reacted with CD4⁻/CD8^high lymphocytes, but it also failed to recognize CD4/CD8 double positive lymphocytes. It was concluded that this mAb is specific for a previously unrecognized CD8 epitope, and was, thus, given the wCD8c designation. A very similar reactivity pattern to that of PG164A was observed for two other mAbs (STH106, #094; and SwNL554.1, #009). Although these two mAbs were not originally positioned in the T cell subgroup because of their reactivity and their ability to inhibit the binding of PG164A, they were given the wCD8c designation. Overall, five new wCD8 mAbs were identified. Although the molecular basis for the differences in PBL recognition by these mAbs is not yet understood, they will be important in defining the role of CD8⁺ lymphocyte subsets in health and disease.  相似文献   

Lowered Antioxidant Status of Red Blood Cells in Post-Parturient Haemoglobinuria of Buffaloes     

Chugh  S.K.  Bhardwaj  R.M.  Mata  M.M. 《Veterinary research communications》1998,22(6):385-388

The antioxidant status of the red blood cells of buffaloes (n = 20) suffering from post-parturient haemoglobinuria (PPH) was assessed by comparing their tocopherol (vitamin E) and reduced glutathione contents with those of red blood cells from apparently healthy buffaloes (n = 20). The red cell tocopherol content of the diseased buffaloes (1.76±0.11 g/ml) was significantly (p<0.01) lower than that of healthy buffaloes (2.45±0.14 g/ml). This may be the first report comparing the concentration of tocopherol in the red blood cells of buffaloes suffering from PPH and apparently healthy buffaloes.There was a drastic reduction in the reduced glutathione content in the red cells of haemoglobinuric buffaloes (23.74±2.86 mg%) compared to the healthy control buffaloes (73.71±3.87 mg%). The diseased buffaloes also exhibited severe hypophosphataemia. These findings suggest that an impaired or insufficient antioxidant potential of the red blood cells in this disease in buffaloes is associated with the phosphorus deficiency.  相似文献