乳山湾东流区细菌数量的分布及与环境因子关系的研究   总被引：1，自引：0，他引：1  

陈碧鹃  李秋芬  陈聚法  马绍赛  崔毅  辛福言 《海洋水产研究》1997,(2)

根据1995年6～9月对乳山湾东流区的微生物调查资料，研究了乳山湾东流区海水及底泥中细菌和弧菌量的变化及其与环境因子的关系。结果表明，该海域海水中细菌和弧菌的数量变化与温度的变化相一致，6月中旬～8月中旬，细菌数量增长比较缓慢，8月中旬开始，细菌数量增长较快，到8月底，达到最高值，异养菌为5．71×106cell／mL，弧菌为2．1×104cell／mL。而后，随着温度的下降，水中的细菌量逐渐减少。滩涂底泥中，细菌和弧菌的数量均比海水中细菌高l～2个数量级。  相似文献   

乳酸杆菌L1对致病弧菌的抑制作用   总被引：7，自引：0，他引：7  

杨莺莺  李卓佳  陈永青  梁晓华  杨铿 《南方水产》2005,1(1):62-65

以致病弧菌T1、T2为检测菌,采用平板抑菌圈检测法,研究乳酸杆菌L1及其代谢产物的抑菌能力。结果表明:L1发酵上清液对两种弧菌的抑制效果和发酵液对弧菌的抑菌作用相似,即对弧菌T1的抑制作用强于T2;L1 发酵液对弧菌T1、T2的抑制能力强于上清液,表明乳酸杆菌及其代谢物质对弧菌具有协同抑制作用;L1的发酵液经3倍稀释仍对弧菌T1有抑制作用;乳酸杆菌L1生长不同时期的代谢产物对弧菌的抑菌活性不同,代谢产物的抑菌能力在衰退期>稳定期>生长期;L1的发酵液经沸水浴处理15 min其抑菌活性不变,表明抑菌代谢物质具有较好的耐热性。  相似文献   

二种佐剂对哈氏弧菌OMPC-溶藻弧菌LPS偶联疫苗的增效作用     

李华  钱永华  石存斌  潘厚军  吴淑勤 《水产科学》2007,26(9):489-493

制备哈氏弧菌SpGY020601外膜蛋白复合物(OMPC)和溶藻弧菌EpGS021001脂多糖(LPS)偶联疫苗,设置偶联疫苗组、偶联疫苗 ISA763佐剂组、偶联疫苗 β-葡聚糖佐剂(DEAE)组以及生理盐水对照组,并以相同程序免疫卵型鲳鲹。溶菌酶活性检测、微量凝集反应试验结果显示:各疫苗组血清中的溶菌酶含量无显著差异(P>0.05),但均远高于生理盐水对照组(P<0.05);ISA763佐剂组抗哈氏弧菌SpGY020601抗体滴度较其他组出现的早,抗溶藻弧菌EpGS021001抗体滴度较其他组持续时间长;β-葡聚糖佐剂组次之,其次是无佐剂组,对照组始终未检测到抗体。各组分为腹腔注射攻毒与浸泡攻毒,对EpGS021001的攻击,ISA763佐剂组相对保护率分别为90%和70%,高于β-葡聚糖佐剂组的85%和55%,以及无佐剂组的80%和55%;对SpGY020601的攻击,ISA763佐剂组相对保护率分别为95%和80%,高于或相当于β-葡聚糖佐剂组的95%和75%,以及无佐剂组的90%和75%。  相似文献   

Delivery of HUFA, probionts and biomedicine through bioencapsulated Artemia as a means to enhance the growth and survival and reduce the pathogenesity in shrimp Penaeus monodon postlarvae     

G. Immanuel  T. Citarasu  V. Sivaram  M. Michael Babu  A. Palavesam 《Aquaculture International》2007,15(2):137-152

One of the major problems in the shrimp culture industry is the difficulty in producing high-quality shrimp larvae. In larviculture, quality feeds containing a high content of highly unsaturated fatty acids (HUFA) and ingredients that stimulate stress and disease resistance are essential to produce healthy shrimp larvae. In the present study, Penaeus monodon postlarvae (PL15) were fed for 25 days on an unenriched Artemia diet (control; A) or on a diet of Artemia enriched with either HUFA-rich liver oil of the trash fish Odonus niger (B), probionts [Lactobacillus acidophilus (C1) or yeast-Saccharomyces cerevisiae (C2)] or biomedicinal herbal products (D) that have anti-stress, growth-promoting and anti-microbial characteristics. P. monodon postlarvae fed unenriched Artemia exhibited the lowest weight gain (227.9 ± 8.30 mg) and specific growth rate (9.95 ± 0.05%), while those fed the HUFA-enriched Artemia (B) exhibited the highest weight gain and specific growth rate (362.34 ± 12.56 mg and 11.77 ± 0.08%, respectively). At the end of the 25-day rearing experiment, the shrimp postlarvae (PL40) were subjected to a salinity stress study. At both low and high (0 and 50‰) salinities, the group fed the control diet (A) experienced the highest cumulative mortality indices (CMI) 935.7 ± 2.1 and 1270.7 ± 3.1, respectively. Those fed diet D showed the lowest stress-induced mortality, and CMI were reduced by 31.1 and 32.3% under conditions of low and high salinity stress, respectively. A 10-day disease challenge test was conducted with the P. monodon postlarvae (PL40–PL50) by inoculating the shrimp with the pathogen Vibrio harveyi at the rate of 10⁵–10⁷ CFU/ml in all rearing tanks. P. monodon postlarvae fed probiont-encapsulated Artemia diets (C1 and C2) exhibited the highest survival (94.3 and 82.3%, respectively) and lowest pathogen load (V. harveyi) in hepatopancreas (5.2 × 10² ± 9.0 × 10 and 4.6 × 10² ± 9.0 × 10 CFU g⁻¹, respectively) and muscle (2.0 × 10² ± 6 × 10 and 1.7 × 10² ± 8.6 × 10 CFU g⁻¹, respectively) tissues. The shrimp that were fed the unenriched Artemia (Control; A) showed the lowest survival (26.33%) and highest bacterial load in the hepatopancreas (1.0 × 10⁵ ± 5 × 10³ CFU g⁻¹) and muscle (3.6 × 10⁴ ± 6 × 10² CFU g⁻¹). The shrimp fed the herbal product (D)-enriched Artemia also exhibited enhanced survival and reduced V. harveyi load in the tissues tested compared to the control diet (A) group. The results are discussed in terms of developing a quality larval feed to produce healthy shrimp larvae.  相似文献   

A review of extracellular virulence product of Vibrio species important in diseases of cultivated shrimp   总被引：2，自引：0，他引：2  

Gabriel Aguirre-Guzmán  Humberto Mejia Ruíz  & Felipe Ascencio 《Aquaculture Research》2004,35(15):1395-1404

Shrimp aquaculture is an important industry that experiences significant losses from Vibrio species, especially at the larval and juvenile stages. Proteinaceous virulence factors, including alkaline proteases, metalloproteases, cysteine proteases and alkaline serine proteases, have been identified as important elements in Vibrio pathogenesis. This review summarizes current knowledge regarding the principal pathogenic Vibrio species in shrimp, with emphasis on relevant exotoxins and their modes of action, principal characteristics and molecular database. This pathogenic factors and their relation with other molecules produced by microorganisms may be help to understand the virulence mechanisms present in Vibrio strain.  相似文献   

Association of a bacteriophage with virulence in Vibrio harveyi   总被引：2，自引：0，他引：2  

Austin B  Pride AC  Rhodie GA 《Journal of fish diseases》2003,26(1):55-58

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台湾输入甲鱼中检出O1群霍乱弧菌   总被引：1，自引：0，他引：1  

吴玉娟  ;孔繁德  ;徐淑菲  ;叶妍妍  ;龚艳清  ;王仍瑞 《经济动物学报》2014,(2):100-103

将甲鱼体表涂抹物在碱性蛋白胨水中进行增菌培养,分离单菌落后分别用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)、血清学方法及PCR进行鉴定,结果从台湾输入大陆的甲鱼中检出O1群稻叶型霍乱弧菌一株,不含ctx基因和toxR基因。为此,应加强水产品尤其是甲鱼的监测工作,防止霍乱弧菌在不同地区间的传播和流行。  相似文献   

养殖大黄鱼溶藻酸弧菌病病原菌传染途径的研究     

刘振勇  林小金 《福建水产》2005,(1):26-30

从养殖大黄鱼病鱼的肝脏和脾脏内分离、纯化到溶藻酸弧菌，经回归感染试验证实它是该病的致病菌。使用法国生物一梅里埃公司生产的自动细菌鉴定仪(ATB)进行细菌鉴定，该菌株的生理生化特性符合溶藻酸弧菌的特性。研究结果表明，养殖大黄鱼溶藻酸弧菌病病原菌传染途径，主要是从水经伤口传染到体内，在试验条件下传染率为60％。病原菌在30℃时生长很快，20℃时生长慢，10℃时基本不生长，对氟苯尼考、四环素和乙酰螺旋霉素等7种药物敏感。  相似文献   

溶藻弧菌单克隆抗体的制备及应用   总被引：7，自引：0，他引：7  

宋晓玲 《水产学报》2001,25(6):522-527

小鼠骨髓瘤细胞系SP2/0与经溶藻弧菌(1.1587)免疫的BALB3/C雌鼠脾细胞在PEG条件下融合,有45.8%的培养孔有杂交瘤细胞生长,其培养上清液抗体阳性率为77.4%.经反复有限稀释法克隆杂交瘤细胞,获得7株抗溶藻弧菌的单克隆抗体杂交瘤细胞株,分别为AC1-C9,AC1-C11,BB4-D4,AD1-A3,AD1-F3,AD2-E7,AD2-F7.取AD1-F3扩大培养,注射小鼠,制备了抗溶藻弧菌的单克隆抗体腹水,滴度为11000.该腹水与其他3株溶藻弧菌菌株有强交叉反应,与其他13株标准菌株无交叉反应.利用制备的单抗腹水,建立了检测溶藻弧菌的单抗-ELISA技术.该反应系统可应用于溶藻弧菌的快速诊断,反应时间为6-7h,检测灵敏度为104cells·-1.并利用该检测方法进行了11份待测菌样本溶藻弧菌的检测.  相似文献   

杂色鲍养殖环境中致病性弧菌分布调查   总被引：3，自引：0，他引：3  

刘广锋  徐力文  黄建荣  王江勇  许丹  陆镜  周世宁 《南方水产》2005,1(3):60-64

从深圳不同的杂色鲍人工养殖场采集并分类环境生物样品。应用TCBS平板分离并作生理生化初步鉴定弧菌,平板涂布法统计水样和各类生物每mL(或每g)所含弧菌总数,并应用基于16S~23SrDNA间区序列设计的4种水产病原弧菌特异性引物进行PCR,定性检测各类环境样品所携带致病性弧菌的分布状况。结果显示,弧菌广泛存在于杂色鲍养殖环境中,且杂色鲍养殖池池水中的弧菌密度大于进水口海水。在养殖环境生物中,不同环境生物中每克生物所携带的弧菌数差别很大,其中盘管虫、海蟑螂、等足类所携带的弧菌数最多,而海鞘携带的弧菌数最少。在常见的4种致病性弧菌的检测结果上,创伤弧菌和溶藻弧菌阳性率均为3.4%。通过研究弧菌在杂色鲍养殖环境中弧菌的分布特征,为杂色鲍养殖中流行性弧菌病的预防提供科学依据。  相似文献