大黄鱼过氧化氢酶基因的克隆及其对鳗弧菌感染的响应   总被引：2，自引：2，他引：0  

严林飞  安昕  包苗苗  何建瑜  吴常文  刘慧慧 《水产学报》2017,41(5):641-648

为探究过氧化氢酶(catalase,CAT)对大黄鱼机体的保护作用,实验基于大黄鱼基因组序列数据库克隆获得CAT基因1584 bp的完整开放阅读框(GenBank登陆号:KKF-14425.1),该序列编码527个氨基酸残基,包含与其他动物高度保守的酶活性中心序列FDRERIPERVVHAKGA、亚铁血红素结合信号序列RLFSYPDTH、3个催化位点残基His75、Asn148和Tyr358,以及12个NADPH结合位点等,理论分子量为59.98 ku,等电点为8.37。多序列比对显示,大黄鱼CAT氨基酸序列与其他鱼类具有较高的一致性,与同属于石首鱼科的军曹鱼和条石鲷同源性高达94%,在进化树中也聚类于同一进化分支,说明该序列为CAT家族成员。实时荧光定量PCR检测显示,大黄鱼CAT基因在所检测的7种组织(肝脏、脾脏、脑、肾、肌肉、鳃、肠)中均有表达,但在肝脏中表达水平最高(为肌肉中的6.68倍)。鳗弧菌感染后,大黄鱼肝组织中CAT基因的表达随着时间的推移而变化明显,感染后12 h,达到最高(7.48倍),随后逐渐下降,到72 h已基本恢复到原始水平,注射PBS的对照组,CAT基因表达只略有上调,说明病原菌侵染可能引起鱼体产生大量活性氧自由基及H2O2,CAT基因则可以清除体内过量的活性氧,进而防止它们对细胞造成损伤。  相似文献   

Prevalence of a fish pathogen, Listonella anguillarum, in the intestinal tract of fish collected off the coast of Japan     

Haruo Sugita  Hirotaka Mizuki  & Shiro Itoi 《Aquaculture Research》2008,39(1):103-105

This study evaluates the influence of temperature manipulation on circulating levels of sex steroids and gonadal maturation in F1 generation Senegalese sole Solea senegalensis breeders kept under either continuous light (LL) or natural photoperiod (NP). Under LL, progressive water temperature reduction during early winter [treatment MT (modified temperature)] significantly elevated [compared with exposure to LL and constant temperature (CT)] plasma steroid levels in females (January–February) and males (January) and proportion of females initiating maturation (January), suggesting a modulatory role of decreasing temperature in the early phases of sole gonadal recrudescence. Under NP, treatment MT did not stimulate female gonadal maturation despite the significantly higher plasma testosterone levels (February) compared with exposure to NP and CT, which may indicate that females at early‐intermediate phases of ovarian development, the predominant stages of maturation at early winter, were less responsive to the treatment. Conversely, treatment MT significantly enhanced male androgen production from January onwards, together with an increase in proportions of running individuals (March), indicating that males were also responsive to this treatment at mid‐late phases of testicular development. Our data further support the strong influence of water temperature on the overall control of gonadal maturation in Senegalese sole.  相似文献   

牙鲆抗鳗弧菌病AFLP分子标记筛选   总被引：13，自引：1，他引：12  

刘云国  陈松林  LIU  Zhan-jiang 《中国水产科学》2007,14(1):155-159

牙鲆(Paralichthys olivaceus)感病群体和抗病群体通过注射鳗弧菌(Vibrio anguillarum)获得。利用61对AFLP引物组合扫描了牙鲆感病群体和抗病群体各20个个体,结果共扩增出3 200条带,8条AFLP带在2个群体中显示了极大的差异(P<0.01),其中有2条带是在抗病群体中出现的高显性基因频率的标记,另外6条带是在感病群体中出现的高显性基因频率的标记。这些标记很可能是与抗病性相关的候选标记。这些抗病性候选标记的获得为实现牙鲆分子标记辅助育种和抗病基因克隆奠定了一定基础。[中国水产科学,2007,14(1):155-159]  相似文献   

Diseases and Injuries Associated with Mortality of Hatchery Reared Baltic Cod (Gadus morhua L.) Larvae     

K. Buchmann  J. L. Larsen  I. Dalsgaard 《Acta veterinaria Scandinavica》1993,34(4):385

A cod hatching plant was established in 1992 on the island of Bornholm in the Baltic Sea in order to elucidate the possibilites for restocking of cod fry in this brackishwater system. The disease prevalence in 3 batches of hatchery-reared yolksac larvae from the Baltic cod (Gadus morhua L.) was monitored during the posthatch period. High prevalences of bacteriosis/mycosis, lordosis/scoliosis, injuries and protozoan endoparasitism were recorded. Vibrio sp. and Vibrio anguillarum serovar 04, 06, 08 in addition to nontypable strains and saprolegniaceous fungi were isolated from the larvae. The dinoflagellate-like endoparasites were located in the yolksac of the cod larvae.  相似文献   

芝罘湾网箱越冬鲈大批死亡探因   总被引：1，自引：0，他引：1  

王为璋 《齐鲁渔业》1994,11(6):9-11

分析了芝罘湾鲈网箱越冬大批死亡的原因，主要由鳗弧菌侵入鱼体所致。提出了预防鲈越冬死亡的措施。  相似文献   

海水养殖斜带石斑鱼致病性鳗弧菌的分离鉴定及药敏试验          下载免费PDF全文

饶颖竹  梁静真  陈蓉  杨廷宝 《南方农业学报》2016,47(8):1416-1422

[目的]了解海水养殖斜带石斑鱼弧菌病的病原,为生产中有效防治石斑鱼弧菌病提供科学依据.[方法]采用常规方法进行患病斜带石斑鱼病原菌分离,人工感染试验确定其致病性,以形态学观察、生理生化测试结合16SrRNA和gyrB基因序列分析等方法进行菌株鉴定,并以K-B纸片扩散法进行药敏试验.[结果]从广东省湛江市某养殖场患病斜带石斑鱼中分离获得l株优势菌株(M1),经人工感染试验证实其对斜带石斑鱼具有较强的致病性.M1菌株的16S rRNA序列和gyrB基因序列与GenBank数据库中的鳗弧菌具有较高相似性,其同源性均在95.7%以上.根据M1菌株的形态特征、生理生化特性及分子特征,可鉴定其属于弧菌属的鳗弧菌(Vibrio anguillarum).药敏试验结果显示,M1菌株对庆大霉素、新生霉素、阿奇霉素、氟苯尼考、利福平、诺氟沙星和左氟沙星等7种药物高度敏感,对阿洛西林、强力霉素、链霉素、复方新诺明和甲氧苄啶等14种药物敏感.[结论]引起广东省湛江市某养殖场海水养殖患病斜带石斑鱼弧菌病的病原菌为弧菌属鳗弧菌,生产中可适度选用氟苯尼考、强力霉素、复方新诺明和甲氧苄啶等抗菌药物进行防治.  相似文献   

Different approaches to expressing Edwardsiella tarda antigen GAPDH in attenuated Vibrio anguillarum for multivalent fish vaccines     

Zheng Y  Xiao Y  Wu H  Wang Q  Xiao J  Zhang Y  Liu Q 《Journal of fish diseases》2012,35(8):569-577

With the development of gene technology, expressing heterologous antigens in attenuated bacteria has become an important strategy to design multivalent vaccines. In our previous work, an attenuated Vibrio anguillarum named MVAV6203 was developed and proven to be an efficient live vaccine candidate. In this research, we aimed to express protective antigen glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) of Edwardsiella tarda in attenuated Vibrio anguillarum to establish a multivalent V. anguillarum vector vaccine. Several strategies were compared between low‐ vs. high‐copy plasmid‐mediated antigen expression, in vivo‐inducible vs. constitutive antigen expression and intracellular vs. surface‐displaying antigen expression. Zebrafish, Danio rerio (Hamilton), was applied as the fish model to evaluate the immune protection of the V. anguillarum vector vaccine candidates. Our results demonstrated that V. anguillarum MVAV6203 (pUTatLNG40), which harbours a low‐copy plasmid‐loaded antigen surface display system under the control of a constitutive promoter, presented the best protective efficacy against the infection of Vibrio anguillarum (relative per cent survival, RPS = 85%) and Edwardsiella tarda (RPS = 70%).  相似文献   

Effect of poly-β-hydroxybutyrate on Chinese mitten crab, Eriocheir sinensis, larvae challenged with pathogenic Vibrio anguillarum     

Sui L  Cai J  Sun H  Wille M  Bossier P 《Journal of fish diseases》2012,35(5):359-364

This study investigated the protective effect of poly-β-hydroxybutyrate (PHB) on Chinese mitten crab, Eriocheir sinensis, zoea larvae challenged with pathogenic Vibrio anguillarum. PHB was delivered to the crab larvae through rotifer and Artemia bioencapsulation. Zoea 3 larvae were challenged with V. anguillarum at a final concentration of 10(5) CFU mL(-1). PHB-enriched rotifers and Artemia nauplii were added to the culture water 24 h prior to, upon and 24 h after challenge. The results confirmed that PHB could enhance the survival and growth of unexposed E. sinensis larvae. Moreover, PHB protected larvae from the pathogen as the larvae fed PHB-enriched live food showed the highest survival and development rate in all challenged groups (P < 0.05). Furthermore, larval performance was the best when PHB was delivered to the larvae 24 h before challenge (P < 0.05). In conclusion, our results indicate that PHB can be used as part of an effective strategy to protect E. sinensis larvae from V. anguillarum resulting in higher survival and better growth, especially when applied before the challenge.  相似文献   

南蛇藤果实提取液对弧菌的体外抑菌作用     

黄晓玲  刘倩倩  杨振美  张现峰  王丙乾  韩晓弟 《北方园艺》2010,(11):173-175

用平板牛津杯法和试管二倍稀释法比较南蛇藤乙酸乙酯相、水相、乙醇相3种提取液对弧菌的抑菌活性和最小抑菌浓度(MIC)。探讨南蛇藤果实提取液对鳗弧菌、副溶血弧菌的体外抑菌作用。结果表明:3种提取液的MIC值分别是145.8、208.32、91.7 mg/mL(鳗弧菌);125.01、87.52、08.33 mg/mL(副溶血弧菌)。南蛇藤果实的3种提取液对鳗弧菌、副溶血弧菌有不同程度的抑制作用,其中乙酸乙酯相提取液抑菌效果最佳,水相和乙醇相次之。  相似文献   

大菱鲆(Scophthalmus maximus L.)鳗利斯顿氏菌(Listonella anguillarum)感染症及其病原特性   总被引：1，自引：0，他引：1  

房海  陈翠珍  张晓君  葛慕湘  何振平  巩元芳 《中国兽医学报》2006,26(2):144-146

对一养殖场所发生的大菱鲆（Scophthalmus maximus L．）病害进行了发病情况、临床表现、病理变化及病原等方面的检验，结果表明为细菌性败血感染症。通过对15尾病（死）大菱鲆病变组织中细菌检查、细菌分离与鉴定，以及人工感染试验的致病作用等，表明所检出的细菌为利斯顿氏菌属（Listonella MaeDonell and Colwell 1986）的鳗利斯顿氏菌[L．anguillarum（Bergeman 1909）MacDonell and Colwell 1986]，系该病例的病原菌。  相似文献