海南辣椒、樱桃番茄病毒病调查及病原dot-ELISA鉴定     

严婉荣  赵志祥  肖彤斌  张善学  文香玲  王会芳 《广东农业科学》2021,48(1):119-125

[目的]调查和鉴定海南省辣椒和樱桃番茄病毒病发生情况和病毒种类,明确病毒病发生为害程度及毒源种类.[方法]2018—2019年,通过5点取样法对海南省10个市县的辣椒和樱桃番茄进行病毒症状类型、发病率和病情指数统计.采集辣椒和樱桃番茄主产区病毒病样本593份,通过斑点酶联免疫吸附法(dot-ELISA)对黄瓜花叶病毒(...  相似文献   

基于ANN的轴流脱粒与分离装置性能指标预测     

朱秀兰  任玉霞  衣淑娟 《黑龙江八一农垦大学学报》2007,19(3):59-63

影响水稻轴流脱粒与分离装置性能指标的因素很多,并且它们之间存在着复杂的非线性关系,用传统方法很难对其进行准确预测。神经网络算法简单、学习收敛速度快、具有线性、非线性逼近精度高等特性。本文以正交旋转组合实验获得的数据作为样本,对人工神经网络(ANN)模型进行训练学习,利用训练后所得到的模型,对性能指标进行了预测。结果表明,网络预测值与实测值之间具有很高的相关性和精确度,为机械性能指标研究提供了一定的理论辅助手段。  相似文献   

花[鱼骨]人工繁殖技术的研究     

徐彦山  管福军  陶忠学  庞才  杨淑杰  毛瑞新  常淑芳  王秀兰  张怡丹 《鲑鳟渔业》2012,(1):10-13

本文主要研究了花[鱼骨]的繁殖生物学和人工繁殖技术。经过实验摸清了花的性成熟年龄和成熟最小型,性腺的发育状况,花的繁殖力、产卵时间和水温、雌雄鉴别等;比较了不同催产药物对花的催产效果,2008年催产率达100%,受精率95.3%,孵化率95%,获鱼苗14.12万尾。  相似文献   

海水鱼类室内育苗与室外育苗对比试验     

杜涛  罗杰  林向阳 《水产科学》2004,23(7):24-26

为建立一套海水鱼类人工育苗技术,分别采用室内水泥池、室外对虾高位池以及室内水泥池与室外对虾高位池相结合的育苗方法进行美国红鱼、花尾胡椒鲷等鱼类人工育苗试验。结果表明:室内水泥池育苗成活率为11%～98 8%,室外对虾高位池育苗成活率最高为6%,室内外相结合育苗的成活率为37 5%～73%。采用前期室内人工培育,幼苗体长约10mm移到室外对虾高位池培育,既可提高育苗成活率,又可降低育苗成本,是目前值得推广的育苗方法。  相似文献   

Aquatic birnavirus induces apoptosis through activated caspase-8 and -3 in a zebrafish cell line     

Hong JR  Huang LJ  Wu JL 《Journal of fish diseases》2005,28(3):133-140

In this study, the possible influence of temperature on infectious pancreatic necrosis virus (IPNV)-induced apoptosis in a zebrafish liver epithelium (ZLE) cell line was investigated. At a lower temperature (18 degrees C), there was expression of viral proteins VP2 and VP3 at 4 h post-infection (p.i.). At this time no expression was found in the high temperature group at 28 degrees C. The cell survival ratio was 52 and 18% at 24 and 48 h p.i., respectively, during IPNV infection at 18 degrees C. In addition, we assayed for apoptosis in IPNV-infected cells with terminal deoxynucleotidyl transferase (TdT)-mediated end labelling (TUNEL) of DNA at different dosages of virus. We found a ratio of apoptotic cells of 8 and 25% at 12 and 18 h p.i., respectively, in the multiplicity of infection (MOI) 1 group. The MOI 10 group had 20 and 45% apoptotic cells at 12 and 18 h, respectively. Furthermore, at 18 degrees C IPNV activated the caspase-8 and 3 from 1.5 to 2 times at 12 and 18 h p.i., respectively. Taken together, these findings suggest that successful virus replication occurs at the low temperature (18 degrees C) compared with the non-permissive temperature of 28 degrees C. Thus, IPNV replication is capable of activating caspase-8 and -3 and inducing host apoptosis.  相似文献   

银鲳繁殖生物学及人工繁育技术的研究进展   总被引：22，自引：8，他引：22  

施兆鸿  王建钢  高露姣  凌建忠 《海洋渔业》2005,27(3):246-250

银鲳是我国主要的经济鱼种之一,开展银鲳繁殖生物学的研究和人工育苗对资源的合理开发利用及海水养殖品种的结构调整、提高养殖效益等具有重要意义。本文根据近年来国内外对银鲳生物学以及育苗等方面的研究内容,归纳了银鲳繁殖生物学及人工繁育技术研究的现状,并提出了今后研究的重点。  相似文献   

An artificial oyster‐shell reef for the culture and stock enhancement of sea cucumber,Apostichopus japonicus,in shallow seawater          下载免费PDF全文

Libin Zhang  Tao Zhang  Qinzeng Xu  Tianlong Qiu  Hongsheng Yang  Shilin Liu 《Aquaculture Research》2015,46(9):2260-2269

Because of its high nutritional value and health benefits, aquaculture production of Apostichopus japonicus in China is the largest of any single species. Therefore, the development of new farming methods is of considerable significance. In this study, discarded oyster shells have been used to create an artificial reef for the culture of this species. The results have shown that from 6th March 2009 to 26th November 2009, the wet weight of sea cucumber increased from 49.57 ± 1.16 to 79.87 ± 1.46 g ind^?1. Between 16th July and 18th October, the specific growth rate and daily weight gain of A. japonicus differed significantly from other periods. Population density was higher within the reef compared with outside the reef area, and the difference was significant (P < 0.01). The maximum distance between A. japonicus individuals within the reef area on 3rd March and 16th July was 65.0 ± 3.3 and 62.9 ± 4.4 cm, respectively, but the difference was not significant (P > 0.05). The diatom species attached to the oyster shells were similar to those found in the stomach content of A. japonicus. In conclusion, the oyster‐shell reef provides a suitable habitat and shelter for the culture of the sea cucumber, A. japonicus.  相似文献   

香鱼嗜水气单胞菌人工染病后血液生理生化指标变化     

范慧慧  张呈念  安钦  董徐辉  李明云 《水产科学》2012,31(5):280-282

从患病香鱼的体腔液中分离获得嗜水气单胞菌病原,经培养稀释的菌液,腹腔注射人工感染健康香鱼,出现典型的出血症后,进行血液生理生化指标的测定。结果发现,病鱼红细胞数量为(1.21±0.08)×1012个/L,血栓细胞数量(189.00±5.19)×1012个/L,较健康香鱼红细胞[(2.41±0.16)×1012个/L]、血栓细胞[(380.0±20.00)×1012个/L]呈极显著降低(P0.01),血红蛋白含量呈显著减少(P0.05),而白细胞数量却显著增加(P0.05)。染病香鱼总蛋白为(47.56±2.63)g/L,健康鱼为(49.23±3.06)g/L,经检验无显著差异;而碱性磷酸酶、Cl-、Ca2+等3项血液生化指标值均呈极显著减少(P0.01),乳酸脱氢酶呈极显著增加(P0.01)。  相似文献   

Production of salmonid amoebic gill disease by exposure to Paramoeba sp. harvested from the gills of infected fish     

D Zilberg  A Gross  & B L Munday 《Journal of fish diseases》2001,24(2):79-82

Atlantic salmon, S almo salar L., were exposed to different concentrations of Paramoeba sp. The lowest concentration which induced amoebic gill disease (AGD) was 230 Paramoeba sp. L^–1 and the severity of infection increased with increasing concentration. The concentration of Paramoeba sp. positively correlated with the number of gill lesions ( R ² > 0.7). This study provides evidence that Paramoeba sp. is the causative agent of AGD and describes an experimental model that enables the severity of the induced disease to be controlled.  相似文献   

红螯螯虾的室内人工育苗   总被引：7，自引：1，他引：7  

顾志敏 《水产学报》2003,27(1):32-37

于1996-1998年,在浙北地区,对澳大利亚引进的红螯螯是进行亲虾培育、人工越冬,怀卵孵化,室内人工育苗技术的研究。研究表明,在浙北地区,2.8-4.9g的幼虾经5个月左右的饲养可以达到性成熟并部分怀卵,利用电厂余热水水泥池人工越冬的成活率可达70%以上。越冬后亲是在水温20℃以上即开始交配怀卵,怀卵盛期4-6月。红螯螯虾一年可产卵4次,但只有第一、二次怀卵可用于育苗生产,个体一次怀卵量较少,一般为400-500粒,但群体怀卵比率较高。试验还表明,红螯螯虾出膜幼体即呈幼虾状,需依附母体7-10d后才营独立生活并开口摄食外源性饵料,所研制的幼虾Ⅰ号饲料为红螯螯虾室内人工育苗较好的开口饲料。室内人工育苗成活率可达60%以上。  相似文献