γ-氨基丁酸对低氧胁迫下甜瓜幼苗抗氧化酶活性及表达的影响     

李敬蕊  杨丽文  王春燕  高洪波  吴晓蕾 《东北农业大学学报》2014,(11)

采用营养液水培方法,研究低氧胁迫下外源GABA对甜瓜幼苗抗氧化酶（SOD、POD、CAT和APX）活性和同工酶表达的影响,采用实时荧光定量PCR方法检测幼苗根系抗氧化酶的基因表达特性。结果表明,低氧胁迫处理下,幼苗根系和叶片抗氧化酶活性和同工酶表达均高于对照,且根系CmSOD、CmPOD1、CmPOD2、CmCAT、CmAPX基因均上调表达;低氧胁迫下外源GABA处理显著提高幼苗根系和叶片SOD、CAT和APX活性以及同工酶表达,且根系CmSOD、CmCAT、CmAPX基因相对表达量在0.5~1 d迅速提高并达到最大值,且增加幅度高于低氧胁迫处理。由此可知,GABA通过促进根系抗氧化酶基因快速大量表达,提高抗氧化酶活性和同工酶的表达量,从而缓解低氧胁迫对甜瓜幼苗的伤害。  相似文献   

11种杀虫剂对松墨天牛肌肉LIM蛋白基因表达的影响     

罗淋淋  林同 《湖南农业科学》2014,(7):33-35

松墨天牛（MonochamusalternatusHope）是松树的重要蛀干害虫,也是松材线虫（Bursaphelenchusxylophilus）的主要传播媒介。试验从松墨天牛文库中分离得到肌肉LIM蛋白基因的部分cDNA序列,长度为871 bp,命名为MaLIM（GenBank：KJ872589）;与赤拟谷盗（Tribolium castaneum）和家蚕（Bombyxmori）氨基酸相似性分别为77%和75%。用RT-qPCR测定了11种杀虫剂对MaLIM相对表达量的影响,结果表明,杀虫剂处理后MaLIM基因的表达量均有所下降,仅为对照的0.03~0.53。  相似文献   

高等植物ACC氧化酶基因启动子研究进展   总被引：2，自引：0，他引：2  

吴健  侯和胜 《天津农业科学》2014,(4):14-16

启动子是控制植物基因表达的重要DNA序列结构,本文从高等植物ACO基因启动子克隆、顺式作用元件分析及GUS基因表达等方面着眼,综述该领域的研究进展。  相似文献   

山葡萄转录因子CBF1基因的克隆及其遗传转化体系     

刘洋 《天津农业科学》2014,(2):7-10

根据山葡萄的CBF1基因序列设计一对特异引物,克隆出山葡萄(Vitis amurensis)栽培品种‘双优’的抗寒转录因子CBF1基因,并将其构建到pBI121表达载体中,获得山葡萄转录因子CBF1基因的植物表达载体,命名为pBI121-ViCBF1,利用农杆菌介导叶盘转化‘巨峰’葡萄以研究高效的‘巨峰’葡萄转化体系。结果表明,以农杆菌LBA4404为介导菌株,以‘巨峰’葡萄叶片为外植体、3 d为预培养时间和4~5 d共培养时间、400 mg·L-1羧苄青霉素,50 mg·L-1卡那霉素筛选浓度转化效果较好,并已筛选到6株转基因植株,PCR检测均为阳性,为提高‘巨峰’葡萄的抗寒性建立了初步的技术基础。  相似文献   

水稻新型育种技术研究现状与展望   总被引：1，自引：1，他引：0  

马小倩  杨涛  张全  张洪亮 《中国农业科技导报》2022,24(1):24-30

水稻是我国主要粮食作物之一,提高水稻产量、改善品质一直是水稻育种研究的重要目标.育种技术的改进有利于育种效率的提高,随着科学技术的迅猛发展,水稻育种技术也在逐步完善.对分子标记育种、转基因育种、基因编辑育种和分子设计育种等目前水稻育种中广泛使用的育种技术进行了总结,并进一步展望了不同育种方法的发展前景,以期为水稻种业发...  相似文献   

猪氟烷基因对繁殖性能的影响初探   总被引：2，自引：0，他引：2  

刘海峰  帅素容  李芳琼  程红  左芳梅  白廷坤 《畜禽业》2003,(9):10-11

本文采用PCR—RFLP技术检测了德阳市种畜场“九五”期间长白猪选育群第三世代123个个体的氟烷基因型,并统计分析了其中80头母猪(NN型52头,Nn型28头)头胎繁殖性状受氟烷敏感基因(Haln)影响的情况。研究结果表明,该猪群中Haln基因的频率为13.82%;NN型个体35日龄断奶成活率显著高于Nn型个体,在其他繁殖性状上二者间无显著差异,研究结果为全面了解Haln基因的遗传效应及其合理利用提供了理论依据和参考。  相似文献   

中国对虾卵黄蛋白原合成部位的研究   总被引：2，自引：0，他引：2  

张成锋  刘红  高祥刚  蔡生力 《海洋水产研究》2006,27(6):7-13

十足目甲壳类卵巢中卵黄的来源,在过去的几十年研究中一直存在争议,内源性合成和外源性合成都有报道。本文以雌性中国对虾为实验材料,根据组织学观察确定其发育阶段,试验虾可以分为:卵巢未发育期;卵黄发生前期;初级卵黄发生期和次级卵黄发生期4个时期。从处于不同卵巢发育期对虾的卵巢和肝胰腺中提取总RNA,用RT-PCR的方法初步探讨不同发育期的中国对虾卵巢和肝胰腺中卵黄蛋白原mRNA的表达,确定是否存在卵黄蛋白原的合成功能。结果发现,从卵巢未发育期到次级卵黄发生期的卵巢和肝胰腺中都有卵黄蛋白原mRNA的表达,说明二者为卵黄蛋白的合成部位。  相似文献   

显微注射生长激素基因导入中国对虾（Penaeus chinensis）受精卵的研究   总被引：4，自引：0，他引：4  

刘萍  孔杰  王清印  李健  杨丛海  梁利群  孙孝文 《中国水产科学》1996,3(4):35-38

本实验采用显微微量注射方法，交羊生长激素基因导入中轻地虾受精卵，受精卵发育到蚤状幼体第三期后采样检测。ＰＣＲ检测结果表明：７个样品共９３尾幼体，有３个样品呈出阳性信号，基因转移比率至少在３％以上。同时斑点杂交结果也证明有两个明显阳性斑点。  相似文献   

Expression of recombinant triangular bream (Megalobrama terminalis) pre-IGF-I in Escherichia coli     

Tong Fudan  Wang Qun  Zhang Haihua 《Fish physiology and biochemistry》2006,32(2):141-145

The insulin-like growth factor I (IGF-I) cDNA (GenBank Accession No. AY247412) of triangular bream (Megalobrama terminalis) was expressed for the first time in Escherichia coli. To construct the expression plasmid, the IGF-I cDNA was subcloned into prokaryotic-expressing vector pGEX-4T-1. The E.coli JM109 was transformed with the recombinant plasmid pGEX-4T-1-IGF-I, and the transgene expression was observed after being induced with isopropyl-β-D-thiogalactoside (IPTG). The results of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting indicated that the recombinant fusion protein had immune activity and the molecular weight was about 47 kDa. The results of SDS-PAGE and thin layer scanning showed that the yield of fusion protein had been enlarged with prolonging time. When the time of induced expression was 1, 2, 3, 4, 5, and 6 h, the expression amount was approximately 1.4, 4.3, 8.1, 11.3, 16.3 and 18.8% of total bacterial protein respectively.  相似文献   

Activin and its receptors in the goldfish     

W. Ge  W. Ge  W. Ge  R.E. Peter  Y. Nagahama 《Fish physiology and biochemistry》1997,17(1-6):143-153

Activin (_AA, _AB and _BB) is a dimeric protein that belongs to the transforming growth factor- (TGF-) superfamily of growth factors and is involved in the regulation of many physiological and developmental processes. Recently, we have demonstrated that porcine activin stimulated goldfish gonadotropin-II (GTH-II) and growth hormone (GH) secretion from dispersed pituitary cells in static culture and pituitary fragments in perifusion. The action of activin in the goldfish is unique in that it has an acute stimulatory effect on the secretion of GTH-II and GH, whereas in mammals activin usually exhibits long-term stimulatory actions on FSH secretion. The action mechanism of activin is different from that of gonadotropin-releasing hormone (GnRH). Using domain-specific antibodies against mammalian activin subunits, we subsequently demonstrated the existence of immunoreactive activin subunits (_A and _B) in the goldfish ovary, testis, pituitary and brain, suggesting endocrine, paracrine and autocrine roles for activin in the regulation of goldfish reproduction. Both activin _A and _B subunits have been cloned from goldfish genome by polymerase chain reaction (PCR). Using the PCR fragments as probes, we have cloned a full length cDNA coding for activin _B subunit from the goldfish ovary. Both activin _A and _B subunits show high homology to those of other vertebrates with the _B subunit much more conserved (93 and 98% identity with human and zebrafish _B subunit, respectively). The identity of the cloned _B subunit was further confirmed by expression in the Chinese hamster ovary (CHO) cells and detection of the specific activity of activin in the culture medium. The messenger RNA of activin _B subunit is expressed in a variety of goldfish tissues including ovary, testis, brain, pituitary, kidney and liver, suggesting a wide range of physiological roles for activin in the goldfish. We have also cloned a full length cDNA coding for the activin Type IIB receptor from the goldfish ovary, suggesting that activin may have paracrine or autocrine actions on the ovarian functions. The identity of the cloned receptor was confirmed by specific binding of¹²⁵ I-activin on COS-1 cells transfected with the cloned Type IIB receptor.  相似文献