Pasteurella multocida isolation in a horse with retropharyngeal infection     

Hlne Amory DVM  PhD  Dip. ECEIM  Daniel Jean DMV  MSc  PhD  Dip. ACVIM  R. Leveille DVM  Dip. ACVR  Robert Higgins DVM  MSc  DSc  Andr Vrins DVM 《Journal of Equine Veterinary Science》2006,26(8):364-369

Retropharyngeal infections in horses normally induce local painful swelling of the retropharyngeal area, which may lead to dyspnea, dysphagia, and systemic manifestations. Differential diagnosis of local painful swelling of the retropharyngeal area includes retropharyngeal lymph node infection, neoplasm, cellulitis, hematoma, guttural pouch empyema, parotiditis, and jugular thrombosis. Apart from Streptococcus equi ssp. equi, other bacteria are rarely reported as a cause of retropharyngeal abscesses. The reason for this might be a lack of specific sampling to identify the causative agent. This work deals with a case of retropharyngeal infection in an 11-year-old Standardbred stallion with acute depression, fever, tachycardia, asymmetric painful swelling in the throat area, ptyalism, and respiratory distress. Endoscopy, radiography, ultrasonography, blood analysis, and cytological examination of a puncture sample taken from the throat mass were consistent with a pyogenic to pyogranulomatous retropharyngeal inflammation. The clinical evolution was initially satisfactory in response to treatment with nonsteroidal anti-inflammatory drugs and antibiotics, but clinical signs relapsed twice, each time a few weeks after cessation of antibiotic therapy. The bacteriologic finding in this case was unusual and consisted of the isolation of a Pasteurella multocida strain that was obtained after the second relapse (ie, 79 days after initial admission), using a brain heart infusion (BHI) medium, and after two successive negative bacteriological cultures performed on day one of clinical signs and at the first relapse of clinical signs, respectively.  相似文献   

水貂隐孢子虫病流行病学调查及虫种的初步鉴定     

王荣军  张龙现  宁长申  艾伟昌  王海燕  朱惠丽  王永立  卢庆斌  李家诚 《中国兽医寄生虫病》2007,15(1):13-16

为初步了解水貂隐孢子虫病的流行情况,作者于2005年11月份用饱和蔗糖溶液漂浮法检查了河北省肃宁县某水貂养殖场的469份粪便样品。结果,8份粪便样品为隐孢子虫阳性,总感染率为1.71%(8/469)。其中,白貂感染率为2.15%(5/233)、灰貂感染率为2.08%(1/48)、黑貂感染率为1.06%(2/188)。所查的8份隐孢子虫阳性样品均来自5~6月龄的水貂,表明幼龄水貂容易感染隐孢子虫病而老龄水貂不易感染。另外,8份阳性样品多数来自雄性水貂,显示水貂的隐孢子虫感染可能存在性别的差异性。根据卵囊形态和大小将水貂隐孢子虫初步鉴定为小球隐孢子虫(Cryptosporidium parvum)。同时,利用所收集的隐孢子虫卵囊进行了小白鼠感染试验,结果表明水貂源隐孢子虫不感染免疫抑制状态下的昆明系小白鼠。  相似文献   

坏死梭杆菌FN(A)p2001株小鼠感染模型的建立   总被引：1，自引：1，他引：1  

苗利光  杨福合  刘艳环  王志刚  肖佳美 《动物医学进展》2007,28(1):35-37

将鹿源坏死梭杆菌FN(A)p2001分离株以3×108、3×107、3×106、3×105、3×104、3×103个/只等不同菌量,分别接种于6组小鼠,每组10只,逐日观察小鼠感染情况.结果表明,3×106、3×107、3×108个/只菌量感染组小鼠,在接种后3 d～8 d先后死亡,5 d后死亡小鼠的病理变化明显,并从死亡小鼠内脏及脓汁中均检到长丝状及小杆状等多形态典型的坏死梭杆菌;3×104个/只和3×105个/只菌量感染仅表现体重减轻,3×103个/只菌量感染无明显临床表现.由此表明,坏死梭杆菌FN(A)P2001分离株具有很强的感染毒力;对小鼠的最小致死量为106个/只菌量;腹腔接种是适宜的感染途径.从而建立了坏死梭杆菌分离株小鼠感染模型.  相似文献   

感染鸡日龄对球虫单卵囊分离的影响     

李赟  古少鹏  王敏霞  王海珍 《中国畜牧兽医》2007,34(5):96-98

选用5、8、11日龄的雏鸡进行鸡球虫单卵囊分离试验,研究不同感染日龄对鸡球虫单卵囊分离的影响。结果表明:在其他条件一致的情况下,从感染成功率、潜伏期、高峰期的出现时间及持续时间和从每只感染雏鸡收集到的球虫卵囊数量等指标进行综合判定,发现感染11日龄的雏鸡单卵囊分离成功率最高。  相似文献   

A dynamic model forecasting infection of pear leaves by conidia of <Emphasis Type="Italic">Venturia nashicola</Emphasis> and its evaluation in unsprayed orchards     

B. -H. Li  J. -R. Yang  X. -L. Dong  B. -D. Li  X. -M. Xu 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(3):227-238

A dynamic model, called VenInf, was developed to forecast infection of pear leaves by conidia of Venturia nashicola. By simulating conidial infection processes following a rain event, the model estimates % conidia that successfully infected leaves at the end of an infection period. The model is mainly derived from logistic models developed from recent laboratory and glasshouse experimental results on infection of pear seedlings to estimate the rates of infection and mortality. It simulates the conidial infection process at 5 min intervals using temperature, relative humidity (RH), surface wetness and rainfall as input. The model was evaluated against pear scab in four unsprayed orchards in China over a 4-year period. In all orchards, all significant disease increases were associated with infection periods predicted by the model. In one orchard, in 2004 the incidence of leaf infection remained very low (<3%) during the entire season despite the model forecasting several severe infection periods. Results of orchard evaluation suggest that the model is able to identify all important potential infection periods. Thus, further field studies should be carried out to determine whether and how the model can be used in practice to assist farmers in making decisions on fungicide applications.  相似文献   

Localized hemibiotrophy in Colletotrichum: cytological and molecular taxonomic similarities among C. destructivum, C. linicola and C. truncatum   总被引：1，自引：1，他引：1  

A. O. Latunde-Dada  J. A. Lucas 《Plant pathology》2007,56(3):437-447

The infection process of hemibiotrophic isolates of Colletotrichum linicola (from flax, Linum usitatissimum ) and C . truncatum (from broad bean, Vicia faba and lentil, Lens culinaris ) was studied by light microscopy. Host surfaces were penetrated directly leading to a symptomless, biotrophic phase characterized by the elaboration of large multilobed, multiseptate, vesicular primary hyphae that were restricted to the initially infected epidermal cells. Biotrophy lasted for the first 48 h of the host-pathogen interaction and was rapidly succeeded by a necrotrophic phase during which narrow, secondary hyphae invaded the surrounding leaf tissues and water-soaked spreading lesions with sporulating, monosetate acervuli were produced on infected host surfaces. Molecular taxonomic analysis of the nucleotide sequences of the amplified D2 and ITS-2 regions of rDNA revealed very close similarities (97–99%) between these isolates and those of C . destructivum obtained from cowpea ( Vigna unguiculata ) and lucerne ( Medicago sativa ), and also of C . truncatum obtained from pea ( Pisum sativum ). This association was consistent with results from a comparative assessment of some in-planta and in-vitro morphological and growth characteristics of these hemibiotrophic fungi. It was concluded that localized hemibiotrophy is an infection strategy utilized predominantly by a closely-related group of pathogens comprising C . destructivum , C . linicola and C. truncatum , and the formation of multilobed primary hyphae restricted to the first penetrated cell might therefore be a key taxonomic character which correlates consistently with ITS sequence data.  相似文献   

Nucleotide sequence homology to bovine viral diarrhea virus 2 (BVDV 2) in the 5′ untranslated region of BVDVs from cattle with mucosal disease or persistent infection in Japan     

M. Nagai  M. Sato  H. Nagano  H. Pang  X. Kong  T. Murakami  T. Ozawa  H. Akashi 《Veterinary microbiology》1998,60(2-4):271-276

Cytopathogenic and non-cytopathogenic bovine viral diarrhea viruses (BVDVs) were isolated from cattle with mucosal disease or persistent infection in Japan. These isolates were compared for antigenic properties by cross-neutralization tests with Japanese reference strains of BVDV belonging to classical type 1. Significantly low cross-reactivity to reference strains was noted, indicating the viruses to possibly represent a new serotype in Japan. Thus, to determine the genotype of the isolates, nucleotide sequences of the 5′ untranslated region were determined and compared with those of previously reported BVDV 1 and 2. The isolates were clearly shown to belong to BVDV 2, not to BVDV 1.  相似文献   

Neonatal Equine Herpesvirus Type 1 Infection on a Thoroughbred Breeding Farm   总被引：2，自引：0，他引：2  

Michael J. Murray  Fabio del  Piero  Stuart C. Jeffrey  Michael S. Davis  Martin O. Furr  Edward J. Dubovi  John A. Mayo 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(1):36-41

Of 17 foals born on a Thoroughbred breeding farm between March and April 1995, infection with equine herpesvirus type 1 (EHV-1) was associated with neonatal morbidity in 5 foals, 3 of which died or were euthanized. Morbidity and mortality were associated with pulmonary inflammation, and EHV-1 was identified in the lungs of the 3 foals that died. All neonatal EHV-1 infections occurred in foals of mares housed in the same pasture and barn. No other clinical manifestations of EHV-1 infection (eg, abortion, neurologic disease, or respiratory disease) occurred during this outbreak. Three foals were treated with acyclovir (1 died, 2 survived), which may have influenced the clinical outcome in the surviving foals.  相似文献   

Streptococcus uberis: A Permanent Barrier to the Control of Bovine Mastitis?     

J. A. LEIGH 《Veterinary journal (London, England : 1997)》1999,157(3):225-238

The prevalence of bovine mastitis has been reduced over the past 25 years due to the implementation of a five-point control plan aimed at reducing exposure, duration and transmission of intramammary infections by bacteria. This has markedly reduced the incidence of bovine mastitis caused by bacteria which show a contagious route of transmission, but has had little effect on the incidence of mastitis due to bacteria which infect the gland from an environmental reservoir. Streptococcus uberis is one such bacterium which is responsible for a significant proportion of clinical mastitis worldwide.The inadequacies of the current methods of mastitis control have led to the search for additional measures, particularly vaccines to prevent intramammary infection by this bacterium. Such an approach requires detailed knowledge of the pathogenesis of intramammary infection. Our understanding of this area has grown in recent years but a lack of information still hampers disease control. Both live vaccines and, recently, crude sub-unit vaccines have shown promise against bovine mastitis due to S. uberis. Vaccines against mastitis must, however, be able to control infection without the participation of a marked inflammatory response. This review provides an overview of the recent advances which have been made in our understanding of host-pathogen interactions which promote infection and disease and highlights areas for strategic research aimed at controlling this bacterial infection.  相似文献   

Experimental infections of a ciliate Tetrahymena pyriformis on ornamental fishes     

Aranya Ponpornpisit  Makoto Endo  Hisashi Murata 《Fisheries Science》2000,66(6):1026-1031

SUMMARY: Experimental infection of Tetrahymena pyriformis was conducted on ornamental fishes with skin wounded by acetic acid treatment. Among fishes used in this experimental infection, guppy Poecilia reticulata , pristella Pristella maxillaris , neontetra Paracheirodon innesi and cherry barbs Puntius titteya proved to be sensitive to challenge, while medaka Oryzias latipes , dwarf gourami Colisa lalia , goldfish Carassius auratus , platy Xiphophorus maculatus and angelfish Pterophyllum scalare were resistant. In catfish Corydoras aeneus infection was unsuccessful. Exposure to Tetrahymena at ≥100 cells/mL at 25 to 30°C and at pH of 6.0 to 8.0 resulted in the successful infection of some sensitive fish species. Histological and bacteriological observations suggest that deeply destroyed skin tissues from the acid-treated method are the primary factors in successful infection.  相似文献