鸡球虫早熟株的选育及其致病性研究     

段晓琴  李亚林  张浩 《塔里木大学学报》2006,18(4):13-16

本试验通过对昌吉地区球虫流行病学的调查,选出昌吉地区鸡球虫病病原的优势种柔嫩艾美尔球虫,应用单卵囊分离技术成功分离出纯种球虫虫株,并对其进行早熟系的初步选育。连续传6代后,选择30只15日龄健康无球虫感染鸡随机分成3组(n=10)(Ⅰ、Ⅱ、Ⅲ组),Ⅰ、Ⅱ组分别口服接种等量(1.5×104个/只)经6代选育的早熟株和亲本株孢子化卵囊,Ⅲ组为不接种空白对照组,根据临床表现、成活率、每克粪便中卵囊数(O.P.G)、病变计分等指标判断早熟株与亲本株致病性差异,以及与不感染对照组的差别来衡量选育结果。结果表明早熟株与亲本株、早熟株与对照组的成活率、每克粪便中卵囊数、病变计分的差异不显著,说明了早熟株的致病性没有明显的降低,可能是选育代数过少所致。  相似文献   

‘早熟红李’在黔北地区的生物学特性及栽培技术     

黎明  金方伦  敖学熙  万敏  李晓松  张发维 《中国园艺文摘》2013,29(2):154-156

历经7年（2005--2012）对黔北地区‘早熟红李’进行引种观察和栽培试验，结果表明：（1）‘早熟红李’具有结果早，果实成熟早，果大，紫红色，甜酸适口等优点；（2）该品种定植后第2年部分植株开花结果，第3年大部分植株开花结果，第4年植株普遍开花结果，第5年以后进入盘果期；（5）该品种植株抗病、抗虫性较强，同时抗低温、抗旱、抗裂果较强。在海拔800～1200m，平地、山地和坡地栽植生长结果均良好。提出高标准建园、配方施肥、合理修剪、合理管理花果、有效防治病虫害和适时采收等配套栽培措施。  相似文献   

黑龙江省饲料油菜品种筛选与种植技术研究   总被引：1，自引：0，他引：1  

毕影东  刘明  周广生  邸树峰  李炜  刘淼  王玲  刘建新  樊超  杨光  傅廷栋  来永才 《中国油料作物学报》2019,41(6):835

为筛选适宜在黑龙江省规模化种植的饲料油菜品种及栽培模式，采用多年多点的田间定位方法，研究了不同品种、不同种植方式和播期的产量差异和施肥效应以及不同收获期产量及营养成分积累规律。结果表明：金油158、15-P15和12-P38的产量表现好；采用机播平作，播种量15 kg/hm2，底肥施二铵150 kg/hm2，4月22日早播，能够显著提高饲用油菜的生物量。盛花期收获，饲用油菜生物量相对较高，与玉米秸秆混合青贮，制成配合饲料较好。本文结果为饲料油菜在我国北方早熟区开展多功能开发利用提供了参考。  相似文献   

Protection of the Reproductive Tract of Young Chicks by Newcastle Disease Virus-induced Haemagglutinationinhibition Antibodies     

Raghul J  Raj GD  Manohar BM  Balachandran C 《Veterinary research communications》2006,30(1):95-102

The present study was conducted to assess the haemagglutination-inhibition (HI) titres required to protect the chicken reproductive tract against direct damage caused by Newcastle disease virus (NDV). Precociously induced oviduct and uterus by oestrogen treatment of young chicks were used to assess the damage or protection against the damage by analysis of ciliostasis or histopathological lesions. Unvaccinated day-old female white leghorn chickens were used as the maternally derived antibody (MDA) group. Chickens were vaccinated with either a live lentogenic vaccine on day 14 of age or, along with it, an inactivated vaccine at day 36 of age, to generate birds with a range of primary or secondary response induced HI antibodies. Birds with different HI antibody levels were challenged with virulent NDV. It was found that a HI antibody titre of 128 and above was protective against direct damage of the reproductive tract, while the 32–64 titre range was protective when derived through secondary vaccination only.  相似文献   

Effect of combined treatment with GnRHa and GH on linear growth in mid- and late pubertal girls at great bone ages with CPP or EFP and relation to CNP signaling pathway     

LI Yin-ya  MA Hua-mei  SU Zhe  CHEN Qiu-li  LI Yan-hong  CHEN Hong-shan  ZHANG Jun  DU Min-lian 《园艺学报》2014,30(10):1855-1860

AIM:To investigate the effect of combined treatment with gonadotropin-releasing hormone analogue (GnRHa) and growth hormone (GH) on the linear growth in mid- and late pubertal girls at great bone ages with central precocious puberty (CPP) or early and fast puberty (EFP), and to determine the relation between C-type natriuretic peptide (CNP) signaling pathway and the accelerative effect of GH on long bone growth in these girls. METHODS:Twenty-two girls were diagnosed as CPP or EFP, whose bone ages were older than 11.5 years with impaired predicted adult height (PAH), and divided into GnRHa treatment group (treated with GnRHa alone, slow-release of triptorelin 60~80 μg/kg every 4 weeks, im) and combined treatment group (treated with GnRHa and GH, 1 U/kg GH every week for 6~7 times, sc). The height, weight and pubertal stage were determined every 3 months. At the beginning and after 6 months of the treatment, the bone age was evaluated and the serum concentrations of amino-terminal pro-C-type natriuretic peptide (NTproCNP), insulin-like growth factor 1 (IGF-1) and procollagen type 1 amino-terminal propeptide (P1NP) were measured. Height velocity (HV), height SD score for bone age (HtSDSBA), PAH and the serum indexes mentioned above were compared at the beginning and the end of the treatment. RESULTS:After 6 months of the treatment, HV, ΔHtSDSBA and ΔPAH of the girls treated with GnRHa+GH were statistically higher than those of the girls given GnRHa alone (P<0.01). Serum concentrations of NTproCNP, P1NP and IGF-1 were not significantly different between the beginning and the end of the 6-month combined treatment. The girls treated with GnRHa alone showed a significant decrease in both serum NTproCNP and P1NP levels (P<0.05) and no significant change of serum IGF-1 level after 6 months of the treatment. CONCLUSION: In the CPP or EFP girls who are in mid- and late puberty and at great bone ages, the combined treatment with GnRHa and GH may accelerate linear growth and improve predicted adult height. This effect of GH is not attributed to the change of serum IGF-1 level, and may be related in part to the acceleration of CNP-mediated long bone growth.  相似文献   

高寒山区钾肥施用量对早熟马铃薯生长性状的影响及效益分析   总被引：3，自引：0，他引：3  

丁海兵  邓宽平  邓禄军  卢扬  雷尊国 《天津农业科学》2011,17(1):68-70

为探索在高寒山区钾肥对早熟马铃薯产量、品质的影响,分析获得最佳经济效益的钾肥施用量,2008年在威宁县以极早熟品种"费乌瑞它"为供试材料,设置不同钾肥施用量的小区试验。结果表明,施用钾肥能够增加产量,每1kg钾肥可增产55.5～89.8kg;在一定范围内,随着钾肥施用量增加马铃薯块茎数、平均单株薯块重、粗淀粉、维生素C含量均呈增加趋势;在适量施用有机肥和氮磷肥基础上,能够获得较好效益的钾肥用量为K_2O 10kg·667m~(-2)。  相似文献   

堆型艾美耳球虫早熟减毒株细胞培养卵囊最佳收获时间的测定     

秦建华  赵月兰  李全福  庞向红  崔平 《河北北方学院学报(自然科学版)》1997,(4)

堆型艾美耳球虫早熟减毒株在鸡胚小肠上皮细胞上培育,卵囊最佳收获时间为120小时。  相似文献   

堆型艾美耳球虫早熟株对常用抗球虫药的敏感性测定     

刘瑞丽  王丽  张李琦 《中国畜牧兽医》2014,41(2):232-235

The study was aimed to explore the sensitivity of Eimeria acervulina （E.acervulina） precocious strain to some common anticoccidials (diclazuril, toltrazuril, sulfamonomethoxine, dinitolmide, decoquinate, nicarbazin, maduramicin, salinomycin and oxytetracycline). The sensitivity of E.acervulina precocious strain to 9 kinds anticoccidials was assessed by using 4 indices: anticoccidial activity percentage (POAA), reduction of lesion scores (RLS), relative oocyst production (ROP) and anticoccidial index (ACI). The results of these sensitivity trials indicated that the four indices POAA, RLS, ROP and ACI of groups toltrazuril, sulfamonomethoxine, dinitolmide, decoquinate, nicarbazin, salinomycin and oxytetracycline were all negative; the ROP index of diclazuril group was positive, the other three indices were negative. The ROP and ACI indices of maduramicin group were positive, the other two indices were negative. The results showed that the Eimeria acervulina precocious strain was sensitive to diclazuril, toltrazuril, sulfamonomethoxine, dinitolmide, decoquinate, nicarbazin, salinomycin and oxytetracycline, it was partly sensitive to maduramicin.  相似文献   

核桃早实基因的SCAR标记     

李伟波  马明  孙翠  侯立群  杨克强 《林业科学》2010,46(3)

根据与核桃早实性状连锁的RAPD标记OPB-08958序列,设计SEA-F/SEA-R和SEB-F/SEB-R2对SCAR引物。在亲本、杂交后代和栽培品种(系)中的检测结果表明:SEA-F/SEA-R引物对在早实亲本‘绿园’中能稳定扩增出SCAR-SEA958特异标记;在‘绿园’ב绿丰’杂交F1后代群体中,SCAR-SEA958标记与核桃早实性状共分离,与核桃早实性状间的遗传距离为1.99cM;用晚实优系‘T-12’和栽培品种‘元丰’及‘T-12’ב元丰’杂交F1后代,检测SCAR-SEA958标记的分离情况,SCAR-SEA958与核桃早实性状共分离;在9个核桃栽培品种(系)中,SCAR-SEA958在8个供试品种的7个早实品种上出现,在1个早实品种‘鲁光’和1个晚实优系‘T-12’上没有扩增出条带。SCAR-SEA958标记在不同遗传背景下有较高的稳定性。  相似文献   

玉米种植技术     

李洪波 《北京农业》2011,(9):23

为收获优良的玉米果实,介绍选用种子、玉米播种、科学施肥、促早熟和病害防治等栽培技术内容。  相似文献