彭泽鲫的分子遗传分析及其与方正银鲫A系的比较   总被引：16，自引：2，他引：16  

李名友 《水产学报》2002,26(5):472-476

Genetic homogeneity between Pengze crucian carp and strain A of silver crucian carp was studied by using transferrin,isozyme and RAPD markers.The studied individuals of Pengze crucian carp showed transferring patterns were the same of silver crucian carp A strain while distinct from those of other crucian carp populations.As far as isozyme is concerned,the MDH,LDH and EST are all of the same with only slight differences in SOD between them.The RPAD patterns clearly indicated high homogeneity among 16 individuals (6 sampled from individuals of two years old and the others aged one) from crucian carp of Pengze and 5 individuals from strain A of silver crucain carp.Nearly indentical banding patterns were observed among all individuals.Average genetic distance within all the individuals is only 0.011,suggesting crucian carp of Pengze might possess indentical genetic background with strain A of silver crucian carp.  相似文献   

Virus infections of the European Eel in North Rhine Westphalian rivers     

Linna Danne  Lisa Horn  Anita Feldhaus  Daniel Fey  Sebastian Emde  Heike Schütze  Mikolaj Adamek  John Hellmann 《Journal of fish diseases》2022,45(1):69-76

Viral infections have been suggested to play a role in the decline of the panmictic population of the European eel (Anguilla anguilla). However, despite the importance of knowledge about pathogenic eel viruses, little is known about their spread in the wild European eel population and only a few eel pathogenic viruses have been described so far. In this study, we aimed to investigate the health status of the A. anguilla stock in North Rhine Westphalia (NRW) State of Germany. For this purpose, we examined tissue samples of 16 elvers, 100 yellow eels and 6 silver eels, sampled from the rivers Rhine, Lippe and Ems. Virus detection was performed via a combination of cell culture and PCR. Next to the detection of frequently encountered pathogenic eel viruses (anguillid herpesvirus 1 and eel virus European X (EVEX)), we isolated the eel picornavirus 1 (EPV-1) from tissue of yellow eels and elvers and demonstrate the distribution of EPV-1 in wild eel population in NRW.  相似文献   

Assessment of the effect of silver nanoparticles on the relevant soil protozoan genus Acanthamoeba     

《植物养料与土壤学杂志》2017,180(5):602-613

Silver nanoparticles (AgNP) are used in a broad range of consumer products and industrial applications. During the regular product life cycle and disposal, AgNP are continuously released into the environment. Hence, the aim of this study was to investigate the potential ecotoxicological effects of AgNP exposure on amoebae. The Acanthamoeba castellanii ATCC 30234 strain and environmental isolate Acanthamoeba strain C5/2, which are both affiliated with genotype T4, were chosen as representatives of ecologically important soil protozoan organisms. The amoebae were exposed to citrate‐stabilized AgNP (30 and 70 nm in size) for 24 h and 96 h at concentrations ranging from 600 µg L⁻¹ to 20 mg L⁻¹. A newly adopted cell culture based microscopic assay was applied to assess the adherence ability of the amoeba trophozoites. The general metabolic activity of Acanthamoeba was determined to be a second independent endpoint by means of intracellular reduction of the redox dye AlamarBlue®. The fate of AgNP within the amoebae and test solutions was visualized by light‐ and transmission electron microscopy (TEM). Both Acanthamoeba strains showed a significant dose‐dependent decrease of adherence ability (p  < 0.04) and metabolic activity (p < 0.01) after 96 h of AgNP exposure. The environmental Acanthamoeba strain C5/2 lost both its adherence ability and metabolic activity at lower AgNP concentrations than the type strain, indicating a higher sensitivity to ionic silver. This was confirmed by the application of AgNO₃, provoking a higher effect level in strain C5/2. AgNP was visualized intracellularly by transmission electron microscopy within the cytoplasm of Acanthamoeba . This is the first report to show the ecotoxicological effects of short‐term AgNP exposure on the soil protist Acanthamoeba , causing both changes in the adherence ability and metabolic activity of this amoeba. This combined approach may be a powerful tool in the future for predicting potential harmful ecotoxicological effects of AgNP exposure using soil protozoans.  相似文献   

Age and growth of yellow eel, Anguilla anguilla (L.), determined by two different methods     

W. R. Poole  J. D. Reynolds 《Ecology of Freshwater Fish》1996,5(2):86-95

Abstract– Ageing and calculation of growth rate of eels has always been controversial, and remarkably little validation has been reported. This article describes the growth of yellow eels from three lakes in the Burrishoole system, Ireland, as determined by back-calculation from otoliths prepared by burning and cracking. Eels captured by fyke net ranged in length from 25.0 cm to 98.5 cm and the ages ranged from 6 to 49 years. Back-calculated growth was slow, with mean annual increments of 2.08 cm, 1.40 cm and 1.97 cm for the three lakes. Yellow eels captured in the Burrishoole system in 1987 and 1988 were marked by Pan Jet inoculated alcian blue dye and released back into the three lakes. Recaptures made over the following three years yielded considerable growth data. Mean annual growth rates recorded were 2.30 cm, 1.07 cm and 0.97 cm. These data compare favorably with the calculated growth rates, thereby validating the otolith analysis for the eels in the Burrishoole system.  相似文献   

海鳗盐渍过程的动力学和热力学     

章银良  夏文水 《农业工程学报》2007,23(2):223-228

该文通过对盐渍过程海鳗肌肉中的食盐、水分、质量和密度的变化来研究氯化钠扩散动力学和热力学，以期了解海鳗盐渍过程的基本机制。结果表明，鱼体体积变化与水分失去，水分失去与食盐获得之间具有良好的线性关系，相关系数分别为0.998和0.994。水分失去和食盐获得随着时间变化的拟合相关系数都为0.992，其平均相对误差分别为4.62%和4.87%，动力学模型能很好地与实验数据相吻合。动力学预示的鱼体体积、水分和食盐含量变化，可作为确定腌制时间的依据。热力学研究表明，盐的扩散系数是温度和初始盐渍浓度的函数,活化能的大小表示了温度对扩散过程的影响，活化能随着初始盐渍浓度增加而减小。当鱼体盐浓度较小时，温度是加速腌制的重要因素。  相似文献   

Soda glass beads as growth medium in plant cultivation experiments. Sodaglasperlen als Kultursubstrat für Experimente mit Pflanzen     

Arne Sandnes  Toril D. Eldhuset 《植物养料与土壤学杂志》2003,166(5):660-661

  相似文献   

太湖猪银染核仁组成区的研究   总被引：2，自引：1，他引：2  

孙有平  夏祖灼 《南京农业大学学报》1993,16(1):72-77

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两种聚丙烯酰胺凝胶银染方法的比较   总被引：20，自引：0，他引：20  

关海涛  徐世昌  郭玉华 《沈阳农业大学学报》2006,37(1):86-87

比较了两种聚丙烯酰胺凝胶的DNA染色方法。对小麦SSR扩增产物的染色结果显示,改进的Bassam银染法颜色背景浅,灵敏度较高,能够看到副带染色。改进的Sanguinetti方法对条带的鉴别力稍差,但因省时省力,成本较低可用于大规模引物筛选时的染色。  相似文献   

STS和GA_3对马铃薯开花结实的影响     

隋启君  姜兴亚  李凤英  奏波  王启明 《内蒙古农业大学学报(自然科学版)》1995,(4)

通过研究早期喷撒硫代硫酸银（ＳＴＳ）和赤霉素（ＧＡ_３）对马铃薯开花，杂交结实和天然结实的影响，揭示出ＳＴＳ处理能有效抑制马铃薯花和否的脱落，ＧＡ_３能促进马铃薯开花，增加花量。对于易落花落蕾的材料，ＳＴＳ和ＧＡ_３处理均能促进花、蕾发育，增加可授粉花、蕾量，保证杂交结实。对于开花性强的材料，ＳＴＳ和ＧＡ_３既有积极的方面又有消极的影响，需慎重使用。  相似文献   

草鱼、鲢、鳙秋繁及其对次年春繁的影响   总被引：1，自引：0，他引：1  

徐庆登  张成安  孙桂尧  刘才兆  钱 《上海水产大学学报》1993,(Z1)

1991年秋催产草鱼、鲂、鲢、鳙亲鱼256组,共获鱼苗5040万尾。平均催产率78.2%,受精率71.9%,孵化率75.4%。秋繁鱼苗当年可育成10cm以上鲢、鳙鱼种和8cm以上草鱼、鲂鱼种。鱼苗到鱼种成活率为37.2%。秋繁亲鱼次年春繁催产率、受精率和孵化率基本不受影响,产卵量比往年未秋繁者低6.8%～9.6%,卵径、卵粒重和鱼苗体长等无显著差异。草鱼、鲢、鳙卵母细胞发育不同步,春繁后经60天左右强化培育,卵巢中未成熟的卵母细胞可发育成熟。  相似文献