马氏珠母贝精子低温保存主要影响因素的研究   总被引：3，自引：0，他引：3  

余祥勇王梅芳  陈钢荣刘雅靖 张春芳 《华南农业大学学报》2005,26(3):96-99

采用二甲基亚砜(DMSO)和甘油作为抗冻剂,以海水或体液等为基础液,配制体积分数为6％、8％、10％和12％的抗冻保护液．将马氏珠母贝精液和抗冻保护液以1：2、1：5、1：10和1：20(体积比)混合后,置4℃中平衡30和60min,再于-18℃中冷冻4、24和72h,解冻后观察精子的活动状态和受精率．结果表明,抗冻剂种类、体积分数以及精液与保护液的比例对精子的活动状态有很大的影响;4℃的平衡时间对精子的存活率影响不显著,但对受精率却有明显的影响．在-18℃时,精液和抗冻保护液体积比1：20,DMSO体积分数为10％和12％的两组保存效果较好．精子存活率随着保存时间的增长而降低,但基础液的类型对精子受精率没有明显影响．  相似文献   

狐精液精子密度简易检测法的初步研究   总被引：1，自引：0，他引：1  

刘志平  白玉妍  张娟茹 《经济动物学报》2005,9(1):4-7

为寻求一种在生产中既简便、快捷又相对准确的检测狐精液精子密度的方法,对来自不同个体蓝狐和银黑狐的多份精液,根据不同的稀释倍数,分别测得了其相应的精子密度、吸光度和视野精子数,从而得出了精子密度与吸光度和视野精子数之间的相关关系。结果表明,精子密度与吸光度之间的回归关系为指数回归,回归关系显著,相关系数高;精子密度与视野精子数之间的回归关系为直线回归,回归关系显著,相关性良好  相似文献   

白点鲑(Salvelinus leucomaenis)精子活力的观察     

徐革锋  刘洋  谷伟  牟振波 《水产学杂志》2012,25(2):28-30,34

在水和BSS激活剂的激活下,白点鲑(Salvelinus leucomaenis)精子的A、B级快速运动时间分别为34.83±2.15 s和36.70±3.26 s;精子寿命分别为120.26±5.75s和117.45±4.33s,激活比例高于98%,BSS的激活效果更好。在4℃和15℃条件下,白点鲑精子活力、激活比例和精子寿命与保存时间呈负相关性,保存1～2h以内,经保存的精子活力和精液质量较好,不影响人工授精效果。  相似文献   

分光光度法测定两种蟹类精子密度的研究     

管卫兵  杨牧川  王成辉 《鲑鳟渔业》2012,(1):35-37,57

蟹类精子计数对其冷冻保存及种群生殖能力的探究有重要意义。为了提高计数蟹类精子数量的速度,建立了分光光度法测定蟹类精子密度的方法。比较了两种蟹类在不同波长（380nm、520nm、760nm）下吸光度（A）与精子密度（C）的关系。结果表明,在520nm波段下三疣梭子蟹（Portunus trituberculatus）精子密度和吸光度之间也存在线性回归关系,其回归方程是：A=0.058×C（R2=0.9928）;中华绒螯蟹（Eriocheir sinensis）精子吸光度与精子密度呈线性回归关系,其回归方程是：A=0.061×C（R2=0.9892）。统计检验表明,使用该方法测定蟹类精子数目,是一种快速而准确的方法,可以加速蟹类精子计数速度。  相似文献   

史氏鲟精子超低温冷冻保存研究     

舒德斌  郭柏福 《水产科学》2012,31(4):232-234

比较了史氏鲟精子在3种不同配比浓度稀释液的保存效果。试验结果表明,配方Ⅲ作为稀释液,8%甲醇作为抗冻剂,二步法超低温(-196℃)冷冻保存,5h后取出,38℃水浴解冻取得最好的冻后激活率,解冻后激活率为(52.3±3.5)%。解冻精子分别采用井水和激活液D(10mmol/L Tris+10mmol/L NaCl+25mmol/L Glu,pH 8.0)激活,进行人工授精。结果显示配方Ⅲ冻精采用激活液D激活授精获得最高受精率为68.56%,最高孵化率为52.91%。本次试验表明,1～2mmol/L范围内,低浓度K+比高浓度K+对史氏鲟精子保存有利;52～82mosmol/kg范围内,高渗稀释液有利于史氏鲟精子的保存;且激活授精方法是影响冻精受精率和孵化率的关键因素之一。  相似文献   

Studies on boar semen. III. Sperm concentration and seminal plasma total solids followed in Danish AI boars through a 10-year-period     

E Blom  P T Jensen 《Acta veterinaria Scandinavica》1984,25(1):107-112

Semen samples from 1531 young boars eligible for AI service were examined for normality. Sperm concentration (s.c.) was determined by the hemocytometer technique in 1348 of the samples. Seminal plasma total solids (t.s.) were determined on all samples in order to control whether the semen samples originated from complete ejaculates.The hemocytometer counts showed an arithmetic mean of 370 × 10⁶/ml with a standard deviation of 188×10⁶/ml. No correlation was found either between s.c. and age or between s.c. and season of the year.The seminal plasma t.s. showed an arithmetic mean of 4.6% with a standard deviation of 1.35%. No correlation was found between t.s. and s.c. or between t.s. and the age of the boars. Neither was there any association between t.s. and the season of the year.Values of t.s. below 1.6% combined with aspermia were regarded as the result of incomplete ejaculations and the following retest from such cases gave ejaculates showing normal values concerning s.c. and t.s. A drop in t.s. combined with an admixture of pathological cells to the ejaculate may indicate an inflammatory condition in the vesicular glands.  相似文献   

家兔显微受精影响因素及显微受精胚胎超微结构   总被引：2，自引：0，他引：2  

李子义  邹啸环 《中国兽医学报》1997,17(6):593-599

以家兔为实验动物，利用胞质内精子注射技术和透明带下精子注射技术，对卵龄、精子获能、注射针管口径、操作室温和操作方法等影响显微受精的因素以及显微受精胚胎的超微结构进行了研究。结果显示：（１）胞质内单精子注射，ｈＣＧ后１５－１７ｈ卵的存活率为６９．４％，显著（Ｐ〈０．０５）高于ｈＣＧ后１９－２１ｈ卵的存活率（４７．３％）；而受精率和卵裂率（分别为５２．９％和４１．２％）分别低于ｈＣＧ后１９－２１ｈ卵的  相似文献   

Sperm Motion Characteristics of Garole Rams Raised for a Prolonged Period in a Semi-arid Tropical Environment     

Joshi A  Naqvi SM  Bag S  Dang AK  Sharma RC  Rawat PS  Mittal JP 《Tropical animal health and production》2003,35(3):249-257

The Garole is a prolific but less well known and rare breed of small sheep found in the hot and humid Sunderban region of West Bengal. An ability to breed throughout the year and to graze in knee-deep water, resistance to foot rot and a strong mothering instinct are some of the special features of this breed. Garole rams could provide germplasm to incorporate prolificacy traits by artificial insemination of the nonprolific sheep breeds found in abundance in the semi-arid and arid tropical climates of India. The aim of the present study was to evaluate the semen production by Garole rams maintained in a semi-arid climate for three years and to objectively assess their semen quality by a computer-assisted sperm analysis technique. The donor rams were randomly selected each year from the original flock procured from their natural habitat or from the offspring born at the Institute farm. Semen was collected weekly for three weeks each autumn for three consecutive years from 8 rams each year. The overall means (SD) of the traits that did not differ significantly with age or year were volume, concentration, curvilinear velocity, average path velocity, amplitude of lateral head displacement, beat frequency, motility and the percentages of rapid motile sperms and of slow motile sperms. The age of the rams had a significant effect (p<0.05) on the straight-line velocity but this was not significantly affected by the length of exposure to the semi-arid climate. However, the age and year had significant effects (p<0.05) on linearity, straightness and the percentage of medium motile sperms. It was concluded that Garole rams are capable of producing good-quality semen even after a prolonged period of exposure to a semi-arid tropical climate.  相似文献   

影响牦牛种间杂交受胎率的免疫学因素   总被引：1，自引：0，他引：1  

何世明  余忠华  冯吉安  王蓉芳  吴贤智 《中国兽医杂志》2000,26(1):4-6

普通牛（冻精）与牦牛种间杂交表现的低受胎率不仅与参配母牦牛的质量,饲养管理,冻精品质,输配技术以及某些生殖器官疾患等因素有关,而且涉及免疫学因素,即存在免疫不孕。研究结果表明：黑白花公牛精液经人工授精多次注入母牦牛生殖道内,能使母耗牛机体产生精子免疫应答;精子抗原存在于精子的头部和尾部;精子免疫以体液免疫反应为主,精子抗体的产生致精子再次进入生殖道时知活甚至凝集,使精子难以上行和受精,因而严重影响  相似文献   

被子植物受精工程概述     

何龙飞  王爱勤  林鉴钊 《广西农业生物科学》1996,(2)

本文简要概述了被子植物受精工程的意义和最新进展，包括试管受精、精子和卵细胞的分离和保存、体外受精等方面的研究成果，并指出了这一领域存在的问题和希望所在。  相似文献