静电场对草莓体内超氧化物歧化酶和脂质过氧化影响的研究     

李玉花  刘福全 《沈阳农业大学学报》1993,24(2):137-141

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Pb,Zn对超富集植物(小鳞苔草)抗氧化酶活性的影响   总被引：5，自引：0，他引：5  

胡宗达  杨远祥  朱雪梅  郝玉娥 《水土保持学报》2007,21(6):86-91

通过人工气候箱盆栽实验,研究Pb,Zn及其复合胁迫对小鳞苔草(Carex gentilisFranch.)根和叶中SOD,POD及CAT活性的影响。结果表明:叶片SOD活性随Pb和Zn浓度增加均呈缓慢上升趋势,根部SOD则表现为有升有降的过程,Pb Zn复合处理可提高根部和叶片SOD活性,最高分别为201.8 U/gFW和233.1U/gFW。根部POD活性随Pb,Zn浓度增加均出现不同程度提高,但叶片POD活性呈有升有降的过程;根部和叶片POD在Pb Zn复合处理中活性增幅明显,最高分别为对照的7.47倍和1.49倍。根部和叶片中CAT在Pb,Zn及其复合处理中活性均受到不同程度的抑制。方差分析显示,Pb对根部3种酶活性影响显著;Zn对叶片POD活性影响显著;Pb Zn互作对植株体内POD和根部SOD影响极显著。经综合分析,小鳞苔草在Pb,Zn及其复合胁迫条件下,SOD和POD起主要作用,并具有耐Pb高浓度胁迫的抗氧化酶活性调控能力,可望在中国西部铅锌复合污染区植物修复中提供一种新资源。  相似文献   

Effects of Ammonium Nitrate and Monosodium Glutamate in Waste Water on the Growth,Antioxidant Activity,and Nitrogen Assimilation of Lettuce (Lactuca sativa L.)     

Maryam Haghighi  Ping Fang  Mohammad Pessarakli 《Journal of plant nutrition》2015,38(14):2217-2229

It has been hypothesized that the uptake of organic as opposed to inorganic nitrogen compounds found in wastewater can be properly substituted for plant nutrients. The objective of this study was to compare effects of applying monosodium glutamate wastewater (MGW) and ammonium nitrate (NH₄NO₃) (AN) on nitrogen metabolism and growth of lettuce. The results showed that while NH₄NO₃ (AN), NO₃^-, nitrite content and NR activity increased the protein content of lettuce. Applying MGW with a high concentration of 17 amino acids and macro- and microelements improved the fresh weights of shoot and root as well as the protein content of lettuce. Antioxidant activities were not affected by AN and MGW, and their interaction effects only increased POD after 8 weeks. In conclusion, substituting a portion of the chemical fertilizers with MGW improved lettuce growth, but did not increase NO₃^- accumulation in leaves.  相似文献   

水杨酸对暖季型草坪草马尼拉过氧化物酶和超氧化物歧化酶活性及其同工酶的影响   总被引：1，自引：0，他引：1  

肖崇德 《湖北农学院学报》2003,23(4):258-260

在冷害胁迫时，用10～1000μmol／L的水杨酸喷施暧季型草坪草马尼拉(Zoysia motrella)，均能提高抗氧化酶系过氧化物酶和超氧化物歧化酶活性，增强消除氧自由基的能力；同时在凝胶电泳图谱上观测到各有一条新的同工酶谱带产生。  相似文献   

家蚕超氧化物歧化酶(SOD)不同品种活性差异的研究   总被引：1，自引：0，他引：1  

刘春  罗英  代方银  陈萍  唐云明  鲁成 《蚕学通讯》2002,22(4):1-6

对西南农业大学家蚕基因库和育种室的114个家蚕品种及遗传系统进行了SOD活性调查，结果表明：SOD活性最高值达237.375U/ml，而最低值为38.372U/ml，平均值为140.08U/ml；品种间SOD活性差异较大，其品种及遗传系统的活性值分布图似正态分布，而一代杂交种的活性值居双亲活性值中间，并推测SOD活性受多基因支配，但也有主基因作用。中国二化性品种SOD活性值比日本二化性品种高20％左右。  相似文献   

超氧化物歧化酶在苜蓿抗寒锻炼过程中的作用   总被引：9，自引：0，他引：9  

魏臻武  王德贤  贺连昌 《草业科学》2006,23(7):15-18

对苜蓿Medicago sativa抗寒锻炼过程中膜透性和超氧化物歧化酶(SOD)活性进行测定,通过苜蓿组织冰冻半致死温度(LT50)说明在抗寒锻炼过程中苜蓿抗寒性的变化。结果表明,在不同低温处理下,苜蓿叶片和根茎组织电解质透出率呈现出S型曲线变化。抗寒锻炼过程中,苜蓿组织LT50从9月初的-6.9℃下降到11月初的-15.6℃,下降了8.7℃。随着秋季气温的下降,苜蓿叶片SOD活性显著增加,11月上旬达到最高峰,此后显著下降。这说明SOD在抗寒锻炼过程中起着重要的保护作用,当苜蓿获得抗寒能力后,SOD活性逐渐下降。在抗寒锻炼过程中,苜蓿根茎SOD活性的变化与叶片中SOD活性的变化规律不一致,苜蓿根茎中SOD活性水平显著高于叶片中的SOD活性水平。聚丙烯酰胺凝胶电泳分析结果显示,不同苜蓿品种具有6条共同的SOD同工酶酶带。适应高寒地区气候条件的当地材料同德杂种苜蓿表现出与其他苜蓿品种较大的特异性。抑制试验表明苜蓿SOD为CuZn-SOD。  相似文献   

Antioxidant role of propolis extract against oxidative damage of testicular tissue induced by insecticide chlorpyrifos in rats     

Azza A. AttiaReda H. ElMazoudy  Nahla S. El-Shenawy 《Pesticide biochemistry and physiology》2012,103(2):87-93

Pesticides induce oxidative stress leading to generate free radicals and alternate the antioxidant or oxygen free radical scavenging enzyme system. This study was conducted to investigate the oral toxicity of chlorpyrifos toward male rat and the oxidative stress of the sub-lethal dose (9 mg/kg; 1/25 LD₅₀) on the lipid peroxidation level (LPO), reduced glutathione content (GSH) and antioxidant enzymes; catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities of testicular tissue. Also, the protective effects of propolis extract (50 mg/kg b.w.) alone or in combination with chlorpyrifos were investigated. The oral administration of chlorpyrifos significantly caused elevation in LPO level by 1.79-fold as compared to control. The activities of antioxidant enzymes including CAT, SOD, GPx and GST were decreased significantly (23.66%, 27.75%, 29.13% and 11.52%) as well as the level of GSH decreased by 21.97% in testicular tissue as compared to control animals. Co-administration of propolis extract with chlorpyrifos or alone in male rats decreased LPO level, normalized CAT, SOD GPx and GST activities, while GSH content was increased in testicular tissue. We conclude that propolis extract significantly reduces chlorpyrifos-induced oxidative stress in rat testis and the protective effect of the pre-treatment with propolis extract as attenuating agent could be due to its antioxidant properties.  相似文献   

6个栽培类型药用菊花超氧化物歧化酶同工酶分析   总被引：2，自引：0，他引：2  

张景景  高致明  张红瑞  夏至  王旭  马彦秋 《河南农业科学》2012,41(4):104-106

为了揭示药用菊花6个栽培类型的遗传多样性,采用聚丙烯酰胺凝胶电泳(PAGE),对不同栽培类型药菊进行SOD同工酶分析。结果表明,6个栽培类型药菊共得到9条SOD同工酶酶谱,其中4条是各栽培类型在4个时期共有的特征酶谱;各栽培类型药菊的SOD同工酶均在8月份表达量最大,酶带总数最多,其中异种大白菊、大洋菊、小洋菊的酶带总数最多,均有8条,贡菊的酶带总数最少,仅有4条;异种大白菊、大洋菊、小洋菊间的亲缘关系最近,贡菊仅与黄金菊亲缘关系较近。6个栽培类型药菊在不同的生长发育时期SOD同工酶遗传变异丰富。8月份是采集样品分析SOD同工酶的最佳时期。利用SOD同工酶谱特征可以找出各栽培类型药菊间的差异。  相似文献   

超氧化物歧化酶与苏云金芽胞杆菌高温诱导产黑色素相关     

刘风侠  杨文君  阮丽芳  孙明 《华中农业大学学报》2012,31(1):39-43

为探讨苏云金芽胞杆菌高温诱导产黑色素机制,利用含mini-Tn10转座子载体pIC333构建苏云金芽胞杆菌油松亚种4CA1,随机插入突变体库,以寻找相关调控基因。通过高温诱导筛选得到1株在42℃产黑色素减弱的菌株;克隆得到mini-Tn10中断基因的部分序列,通过测序及同源性比对分析,确定中断基因为超氧化物歧化酶基因sodA2。  相似文献   

Effects of α-lipoic acid, vitamins E and C upon the heat stress in Japanese quails     

Halıcı M  Imik H  Koç M  Gümüş R 《Journal of animal physiology and animal nutrition》2012,96(3):408-415

Antioxidant vitamins are commonly used as colorant, preventive, sweetener, nutritive and antioxidant materials in food industry. Thus, many studies have been conducted in recent years. We have performed the present study to understand the effects of α-lipoic acid (ALA), vitamin C and E, added into rations of Japanese quails, on chronic heat stress. This study was carried out in five groups as: control, stress, ALA, vitamin E and vitamin C groups. Heat stress is applied to all groups except the control group. Superficial pectoral muscles tissues were used for biochemical determinations. During the research, it is found that heat stress exerted undesired effects such as increasing lipid peroxidation (LPO) level indicating oxidative stress in Japanese quails. In addition, superoxide dismutase (SOD) and catalase (CAT) enzyme activities and glutathione (GSH) amounts were studied to understand the behaviour of defence mechanism. While stress increased LPO ratio, it was determined that all antioxidant added into the ration decreases LPO significantly (p < 0.05). In addition, it was determined that all of antioxidants added into ration effectively restored SOD activity and LPO ratio which heat stress affected negatively. Interestingly, vitamin C did not adjust GSH ratio in contrast to vitamin E and ALA, where vitamin E and C did not perform any positive effect on heat stress decreased CAT activity. Finally, it can be thought that antioxidant vitamins relatively ameliorated these undesired affects caused by stress factors given.  相似文献