代用蛋壳培养体系对鸡胚骨组织中碱性磷酸酶活性的影响   总被引：2，自引：0，他引：2  

李赞东  樱井富士 《畜牧兽医学报》1997,28(1):44-48

以双黄鸡蛋的蛋壳为代用蛋壳，将正常孵化７２ｈ的鸡胚转移至其中进行孵化，取孵化至１０、１２、１４、１６、１８和２０ｄ的鸡胚，测定其骨中碱性磷酸酶（ＡＬＰ）的活性。同时分析正常孵化胚骨中ＡＬＰ的活性做为对照。结果表明：（１）对照组和实验组的ＡＬＰ活性均随着胚胎日龄的增加而升高，尤其是１６ｄ后上升得很快；（２）１４日龄以前的鸡胚，实验组与对照组相比，ＡＬＰ活性无明显差异；（３）孵化后期同日龄鸡胚相比，实  相似文献   

大果沙棘物候及生长节律观测     

房用  王太明  刘得玺  吴兴梅 《江苏林业科技》2003,30(3):23-25

针对山东滨海盐碱地造林树种缺乏的状况,引进了大果沙棘新品种15个,并对其物候期和生长节律进行观测,为大果沙棘新品种选择及其在盐碱地开发利用提供理论依据。  相似文献   

苹果属植物对苹果褪绿叶斑病毒病的抗性     

曾明 《果树学报》1994,(1)

毒原P204双芽嫁接接种苹果褪绿叶斑病毒(Apple Chlortic Leaf Spot Virus),对所用试材都能进行有效浸染.试验表明,苹果属植物受CLSV浸染后,过氧化物酶活性及同工酶都产生显著变化,并且相对酶活性的大小及同工酶的变化与植株对CLSV抗性的强弱显著相关;受侵染后新酶带的出现不是新酶成分的产生,只是由于病毒的侵染诱发酶蛋白的提前合成.试验初步发现所用试材中河南海棠、丽江山定子、小金海棠抗性相对较强;垂丝海棠、锡金海棠抗性较弱;扁果海棠、森林苹果R12740—7A、湖北海棠、三叶海棠则甚敏感.  相似文献   

Plasma cortisol in the horse, diurnal rhythm and effects of exogenous ACTH.     

M Larsson  L E Edqvist  L Ekman  S Persson 《Acta veterinaria Scandinavica》1979,20(1):16-24

Peripheral blood plasma Cortisol concentration and its diurnal variation was measured in 4 horses. Mean concentration of Cortisol during 24 hrs. was 42 ng/ml (s ± 20 ng/ml). Peak values occurred at 6 a.m. and the lowest values were observed at about 6 p.m. (mean 65 ng/ml and 20 ng/ml, respectively).Long-acting ACTH at a dose of 150 i.u. was given by intramuscular injection to the 4 horses. Peak Cortisol concentrations markedly exceeding the prestimulation level were obtained between 2 and 4 hrs. after injection. During the immediate 24 hrs. after these peaks, the mean Cortisol level was markedly lower and the cyclic variation out of phase with the basal diurnal pattern. After a gradual adjustment during the second postinjection day, no differences could be seen between the 2 patterns on day 3 after injection.  相似文献   

大猿叶虫卵孵化的时辰节律研究   总被引：1，自引：0，他引：1  

徐强  张庆 《江西植保》2007,30(3):99-100

在20℃,22℃,25℃,28℃和30℃,LD14:10下观察卵孵化的时辰节律。结果表明,在这五种温度下,卵孵化呈现两个孵化高峰:第一个高峰出现在6:00-6:59,也就是黎明这段时间;第二个高峰出现在19:00-19:59,也就是黄昏这段时间,但此时的孵化率明显低于前者。  相似文献   

A simple method for monitoring the respiratory rhythm in intact insects and assessing the neurotoxicity of insecticides     

Georgia Zafeiridou 《Pesticide biochemistry and physiology》2006,86(3):211-217

A simple, non-invasive method, for the monitoring of the respiratory rhythm of insects was developed. The insect was restrained and a force displacement transducer attached to one abdominal segment of the dorsal region was used to monitor the respiratory rhythm. Although the method was applied to four species of insects, the main study focused on the yellow mealworm beetle, Tenebrio molitor, due to the simplicity of the recorded respiratory rhythm. In this case, the recorded rhythmic activity represents the synchronised contraction of the dorsal abdominal muscles, driven by the respiratory central pattern generator (CPG). This non-invasive method allows prolonged and stable recording of the respiratory rhythm. The records showed a decrease in the amplitude and the duration in the rhythmic contractions to about 25 and 31% of the initial values, respectively, in the first 7 h of continuous recording. The respiratory rhythm was strongly influenced by the presence of the CO₂, creating the CO₂-reflex. In the beginning of the application of CO₂, there was total inhibition of the respiratory rhythm and then there was a gradual increase in the tension developed by the dorsal abdominal muscles. Using this recording method, it was possible to quantify the function of the respiratory CPG, measuring the amplitude of the respiratory contractions, under normal conditions and in the presence of insecticides. The insecticides imidacloprid and deltamethrin of certain quantities were diluted in the appropriate solvent to make 50, 100 and 500 ng per insect, and were applied to the cuticle, topical application, of the immobilized insect, while recordings of the respiratory rhythm were made continuously.  相似文献   

解读朱文颖小说的几个关键词     

刘蓓 《四川畜牧兽医学院学报》2007,5(5):118-122

朱文颖的小说强调凸现小说与不可言说之物（即我们生命本身经常会面临的障碍）进行的对质、理解与包容；反复出现的意象．既勾勒出一个阴柔古雅的背景．又是推动故事发展的有效手段，更是主人公若即若离、稍纵即逝的心理意绪的揭示与固有心理结构的象征；任何具形或抽象的现实事物，都被作家丰富的想象力转化成了种种经验性的阐述，成为极具个人色彩的感觉化抒写；一种贯穿于小说始终的缓慢、凝滞、缠裹的叙述节奏愈加凸显了这种感觉化书写。  相似文献   

黄河源区草地早熟禾生长节律的研究   总被引：2，自引：0，他引：2  

杨时海  马玉寿  施建军  王彦龙  严青 《青海畜牧兽医杂志》2006,36(4):8-10

通过对黄河源区草地早熟禾生长节律的研究,论述了草地早熟禾生育期、分蘖、株高及地上生物量的发育规律和动态。结果表明:草地早熟禾在海拔3 780m的果洛州玛沁县一般于4月下旬返青,9月上旬成熟,生育期为134d左右;分蘖数与单株分蘖数在成熟期达到最大分别为240株/0.25m2,7.5个/株,分蘖速率在成熟期最大为0.143个/株.d-1;株高到成熟期达到93.6cm,日均增高在拔节—抽穗期最大为2.33cm;地上生物量在成熟期达到最高为530g/m2,日均增重在拔节—抽穗期达到最大值为7.474g/m2。  相似文献   

圈养条件下梅花鹿初夏昼间行为节律     

吕慎金  杨燕  刘宇庆  杨林  彭威  魏万红 《家畜生态》2008,29(1):77-82

2006年4月3日至5月27日,以江苏省扬州市瘦西湖景区平山堂养殖场圈养梅花鹿（sikadeer）为研究对象,采用扫描取样法（Scansampling）和全事件记录方法（All-occurrence recording）,研究了34只梅花鹿行为节律。结果表明：初夏昼间梅花鹿取食、卧息、观望、反刍、移动、修饰行为频次依次减少,取食和卧息行为频次占昼间行为的68%。昼活动节律中,取食频次有两个高峰期,分别在7：30和17：30左右。而反刍和卧息行为频次高峰期在11：00～14：00之间。对公鹿、母鹿和幼鹿群体取食和卧息行为频次分析表明,在11：30～12：30、13：30～14：30和15：30～16：30三个时间段内,公鹿取食频次和母鹿、幼鹿取食频次差异显著,在8：30～9：30和11：30～12：30及15：30～17：30连续时间段内,公鹿的卧息行为频次与母鹿和幼鹿卧息行为频次差异显著。本结论可为梅花鹿饲养管理和遗传选育提供基础理论依据。  相似文献   

Evaluation of client-specific outcome measures and activity monitoring to measure pain relief in cats with osteoarthritis   总被引：1，自引：1，他引：0  

Lascelles BD  Hansen BD  Roe S  DePuy V  Thomson A  Pierce CC  Smith ES  Rowinski E 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(3):410-416

BACKGROUND: There are no validated systems for measuring pain from osteoarthritis in cats. HYPOTHESIS: Owner subjective assessments and an activity monitor (AM) can be used to detect pain in cats with osteoarthritis and to assess efficacy of treatments. ANIMALS: Thirteen cats older than 10 years old, with owner-assessed decreases in activity, painful arthritic joints, and clinically normal blood work were included and evaluated for 3 weeks. METHODS: A collar-mounted AM measured activity and a client-specific outcome measure (CSOM) questionnaire characterized the severity of impairment. Overall global quality of life was also evaluated for each treatment. In weeks 2 and 3, meloxicam (0.1 mg/kg, day 1; 0.05 mg/kg, days 2-5) or a placebo was administered in a blinded, randomized, cross-over manner to test the assessment systems. RESULTS: The cats had a median of 4 arthritic appendicular joints. Activity counts for the week when cats (complete data on activity; n=9) were administered meloxicam were significantly higher than at baseline (P = .02) but not after placebo (P = .06). Baseline activity counts were not significantly different from placebo (P = .6). The CSOM data (n=13) showed that owners considered their cats to be more active on meloxicam compared with baseline (P = .001) and placebo (P < .004), and more active on placebo than at baseline (P < .01). Global quality of life improved significantly with meloxicam (P < .042). CONCLUSIONS AND CLINICAL IMPORTANCE: Both an AM and a CSOM system can detect behavior associated with pain relief in cats that are arthritic. Objective activity data might allow subjective assessment systems to be validated for use in clinical studies.  相似文献